##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545614_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1384658
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92389095357843	31.0	31.0	34.0	31.0	34.0
2	32.61083242215767	34.0	31.0	34.0	31.0	34.0
3	32.55129931001012	34.0	31.0	34.0	31.0	34.0
4	36.00121690699075	37.0	35.0	37.0	35.0	37.0
5	35.76284324360239	37.0	35.0	37.0	35.0	37.0
6	35.76642896657514	37.0	35.0	37.0	35.0	37.0
7	35.74178028076247	37.0	35.0	37.0	33.0	37.0
8	35.70171840266694	37.0	35.0	37.0	33.0	37.0
9	37.92833609454465	39.0	38.0	39.0	35.0	39.0
10	37.90402106512944	39.0	38.0	39.0	35.0	39.0
11	37.72999181025206	39.0	38.0	39.0	35.0	39.0
12	37.21223146798704	39.0	37.0	39.0	33.0	39.0
13	36.93464595589669	39.0	37.0	39.0	33.0	39.0
14	37.30058469311555	39.0	35.0	41.0	33.0	41.0
15	37.05274732099912	39.0	35.0	41.0	32.0	41.0
16	37.134357364778886	39.0	35.0	41.0	32.0	41.0
17	37.32837133790438	39.0	35.0	41.0	32.0	41.0
18	37.436055690285976	39.0	35.0	41.0	33.0	41.0
19	37.38907658064302	39.0	35.0	41.0	33.0	41.0
20	37.358463967275675	39.0	35.0	41.0	32.0	41.0
21	37.34069062541075	39.0	35.0	41.0	32.0	41.0
22	37.2052896816398	39.0	35.0	41.0	32.0	41.0
23	37.15261602504012	39.0	35.0	41.0	32.0	41.0
24	37.10217541082347	39.0	35.0	41.0	32.0	41.0
25	37.06457117930926	39.0	35.0	41.0	32.0	41.0
26	36.99170119986307	39.0	35.0	41.0	32.0	41.0
27	36.920612165603345	39.0	35.0	41.0	32.0	41.0
28	36.8046976220843	38.0	35.0	41.0	32.0	41.0
29	36.71699726575082	38.0	35.0	41.0	31.0	41.0
30	36.67463445847278	38.0	35.0	41.0	31.0	41.0
31	36.61550144512219	38.0	35.0	41.0	31.0	41.0
32	36.54631829664798	38.0	35.0	41.0	31.0	41.0
33	36.40427094632754	38.0	35.0	41.0	31.0	41.0
34	36.26582665177972	38.0	35.0	41.0	30.0	41.0
35	36.194497124921824	38.0	35.0	41.0	30.0	41.0
36	36.09512096127708	38.0	35.0	40.0	30.0	41.0
37	35.95887071031258	38.0	35.0	40.0	30.0	41.0
38	35.85286186191825	38.0	35.0	40.0	30.0	41.0
39	35.774276391715496	38.0	35.0	40.0	30.0	41.0
40	35.73848271558753	38.0	35.0	40.0	29.0	41.0
41	35.741036414768125	37.0	35.0	40.0	29.0	41.0
42	35.58360331576461	37.0	34.0	40.0	29.0	41.0
43	35.51160936491177	37.0	34.0	40.0	29.0	41.0
44	35.41564415184111	37.0	34.0	40.0	28.0	41.0
45	35.35456192070533	37.0	34.0	40.0	28.0	41.0
46	35.30747087006322	37.0	34.0	40.0	28.0	41.0
47	35.182043508216466	36.0	34.0	40.0	28.0	41.0
48	35.15972680618608	36.0	34.0	40.0	28.0	41.0
49	35.102350183222136	36.0	34.0	40.0	28.0	41.0
50	34.986276755704296	36.0	34.0	40.0	28.0	41.0
51	34.82934991889694	36.0	34.0	39.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	5.0
10	2.0
11	16.0
12	11.0
13	13.0
14	11.0
15	27.0
16	61.0
17	146.0
18	319.0
19	621.0
20	1194.0
21	2064.0
22	3305.0
23	4914.0
24	7517.0
25	10506.0
26	14480.0
27	17678.0
28	20170.0
29	23835.0
30	28996.0
31	35784.0
32	44558.0
33	57580.0
34	96843.0
35	215442.0
36	95735.0
37	126601.0
38	190835.0
39	385214.0
40	170.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.600684067834801	91.96783610104444	3.035262136932008	3.396217694188745
2	3.14806977607467	2.255358362859276	91.66805088332282	2.928520977743241
3	28.142761606115013	15.231703424239054	43.47412862959662	13.151406340049313
4	26.59595365787075	14.933579266504799	45.24135201616573	13.229115059458726
5	24.267436435567483	14.090338552913426	46.71788990494404	14.924335106575054
6	21.441323417045943	13.975147653788877	49.64879414266917	14.934734786496016
7	21.849221973945912	16.67335905328247	45.962685370683594	15.514733602088024
8	23.574268880835554	19.204381154046704	45.14392723690615	12.077422728211587
9	82.84868899034996	3.5712067528588283	9.008000531539196	4.572103725252012
10	84.50259919778024	3.5089531133319563	8.612234934547015	3.376212754340783
11	78.56221536292716	5.422999758785202	9.922666824587733	6.0921180536999024
12	47.00756432274251	19.579708491194214	16.104409897606484	17.30831728845679
13	38.99569424363272	21.66195551536914	22.850552266335804	16.491797974662337
14	32.44692913340334	24.134407196578504	25.234101128220832	18.184562541797327
15	19.537459791515303	34.46858357803876	28.69127250194633	17.3026841284996
16	14.511308929714053	39.29432394136314	30.09522929127626	16.099137837646552
17	14.616822348912152	24.23269861583149	43.6711447880993	17.47933424715706
18	19.061746655130726	20.384311505079232	41.00276024837902	19.55118159141102
19	18.719929397728535	20.718545662539054	29.209956538004327	31.35156840172808
20	22.470458409224516	22.10307527201663	33.3088026068531	22.117663711905756
21	27.5624739105252	22.87214604617169	28.945270240015947	20.620109803287164
22	28.21628156555626	21.752519394680853	27.352891472118024	22.67830756764486
23	23.51374852129551	25.979844842553177	29.45817667611786	21.048229960033453
24	25.843926803586154	21.713592814976696	25.94409594282487	26.498384438612277
25	23.520392761245017	26.561649158131466	23.685848779987552	26.23210930063597
26	23.651544280248263	21.41496311724628	32.8477501303571	22.085742472148358
27	24.877045450934453	21.710342915001394	31.353157241716005	22.059454392348147
28	20.77711608209392	29.724596254093065	26.677201157253272	22.821086506559745
29	20.68886324276464	30.416391628835427	26.29891280012826	22.595832328271676
30	19.583536151165127	27.54333561067065	32.67868311164201	20.19444512652222
31	18.906906976307507	26.147323021280343	30.379631649114803	24.566138353297347
32	22.618364968100426	27.48072087114652	30.71718792654937	19.18372623420368
33	26.961097975095655	26.473974078797795	25.206874188427758	21.358053757678793
34	27.096582694065972	26.2500920804993	25.716602944553816	20.93672228088091
35	27.73926846918156	25.075578229425606	24.574949193230385	22.61020410816245
36	22.43023186953024	25.693781424727263	27.351302632130096	24.524684073612402
37	22.372889189966042	25.55497458578219	29.242383317757888	22.829752906493876
38	29.489375715880744	25.59065126551105	24.664574212549237	20.25539880605897
39	22.86514070622493	27.775306248907672	29.220067337927485	20.139485706939908
40	21.958996373111628	27.311076092435822	28.133228566187462	22.596698968265088
41	21.273123038324265	29.00687389954776	25.913041343060883	23.80696171906709
42	25.504926126162562	23.57831320080482	26.25709742044606	24.659663252586558
43	21.605768355796158	23.640061300335535	27.203829393250896	27.550340950617407
44	22.5943157082832	24.544761233459813	26.400887439353255	26.460035618903728
45	21.424135057176574	23.63738916035584	27.805999748674402	27.132476033793186
46	22.64920290786606	26.97951407495569	27.770973048940604	22.600309968237646
47	19.219186253934183	30.48861162828655	25.274472107914015	25.017730009865254
48	21.881937633697273	27.288904552604325	26.488490298687474	24.340667515010928
49	22.5847104483562	26.16631688113599	29.025073339409442	22.223899331098366
50	23.31637126279558	22.315474290402395	31.794926978358557	22.57322746844347
51	21.429407117136506	22.999181025206227	28.905765900316176	26.665645957341088
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1030.0
1	1148.0
2	1266.0
3	3596.0
4	5926.0
5	4037.5
6	2149.0
7	2274.5
8	2400.0
9	2746.5
10	3093.0
11	3246.5
12	3400.0
13	3258.5
14	3117.0
15	3397.0
16	3677.0
17	3492.5
18	3308.0
19	3514.5
20	3721.0
21	4184.5
22	4648.0
23	5597.5
24	6547.0
25	7169.0
26	9609.0
27	11427.0
28	12024.5
29	12622.0
30	15398.5
31	18175.0
32	25740.5
33	33306.0
34	34726.5
35	36147.0
36	45116.0
37	54085.0
38	65913.5
39	77742.0
40	94261.5
41	110781.0
42	121335.5
43	131890.0
44	134089.0
45	136288.0
46	142528.0
47	148768.0
48	140976.0
49	133184.0
50	110698.0
51	88212.0
52	78602.5
53	68993.0
54	63658.5
55	58324.0
56	58116.5
57	57909.0
58	52924.5
59	47940.0
60	44161.5
61	40383.0
62	33341.0
63	26299.0
64	20582.0
65	14865.0
66	12536.5
67	10208.0
68	7977.5
69	5747.0
70	5337.0
71	4927.0
72	3515.5
73	2104.0
74	1728.0
75	907.5
76	463.0
77	415.5
78	368.0
79	199.5
80	31.0
81	19.0
82	7.0
83	18.0
84	29.0
85	18.0
86	7.0
87	3.5
88	0.0
89	0.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1384658.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.879927563261477
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.49368022752115	10.489408554532325
2	11.755939505121077	3.498550565485712
3	5.13946115062245	2.294244289063756
4	2.911236015316604	1.7327592410987618
5	1.7912391931549163	1.3326754721310043
6	1.2180852520541903	1.0875012189866067
7	0.827224716163474	0.861633069853642
8	0.5973805121610067	0.7111182998927857
9	0.451195409371731	0.604237950749472
>10	2.7330670667311217	7.989988469304922
>50	0.4989542155253185	5.340934136219639
>100	1.5158472459871306	55.53873245635914
>500	0.05598022905861272	4.935525538493052
>1k	0.010222476610703192	3.0115549878032493
>5k	4.867846005096758E-4	0.5711357500259427
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	7885	0.5694546956721444	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3724	0.2689472779560007	No Hit
ATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG	3507	0.2532755380751059	No Hit
ATGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT	3147	0.22727633827269983	No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	2867	0.20705473842638397	Illumina Single End Adapter 1 (95% over 21bp)
ATTGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC	2481	0.17917781863824858	No Hit
ATGCCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC	2439	0.1761445786613012	No Hit
ATGTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC	2254	0.16278387876284253	No Hit
ATATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC	2119	0.15303417883694026	No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	2038	0.14718435888139886	No Hit
ATTGGTGGGTTGGTGAGACAATCAGATCGATAAATTGATTAAATGCTCCTA	1935	0.13974569893793268	No Hit
ATGGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC	1895	0.13685689895988756	No Hit
ATAGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC	1831	0.13223481899501538	No Hit
ATTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC	1760	0.1271071990339853	No Hit
ATGTTTCGGGGTAACAAAACTATTTGCCAGAGAACTACTAGCCATAGCTTA	1391	0.10045801923651905	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22655413827818854	0.0
2	0.0	0.0	0.0	1.0401124320951456	0.0
3	0.0	0.0	0.0	1.4169563892311314	0.0
4	0.0	0.0	0.0	1.87656446573811	0.0
5	0.0	0.0	0.0	3.5937393926875805	0.0
6	0.0	0.0	0.0	3.9679112098438747	0.0
7	0.0	0.0	0.0	4.551015485412282	0.0
8	0.0	0.0	0.0	6.402808491338655	0.0
9	0.0	0.0	0.0	6.633479169585558	0.0
10	0.0	0.0	0.0	7.083915306162243	0.0
11	0.0	0.0	0.0	7.412732963663229	0.0
12	0.0	0.0	0.0	8.34559869657345	0.0
13	0.0	0.0	0.0	9.189778270157685	0.0
14	7.2219999451128E-5	0.0	0.0	9.545606207453392	0.0
15	1.44439998902256E-4	0.0	0.0	9.717850906144333	0.0
16	1.44439998902256E-4	0.0	0.0	9.817081185390183	0.0
17	1.44439998902256E-4	0.0	0.0	9.969465384232063	0.0
18	1.44439998902256E-4	0.0	0.0	10.3623421812462	0.0
19	2.88879997804512E-4	0.0	0.0	10.869759897389825	0.0
20	3.6109999725564E-4	0.0	0.0	11.493524032649217	0.0
21	5.05539996157896E-4	0.0	0.0	11.823136110144166	0.0
22	5.05539996157896E-4	0.0	0.0	12.284621906636874	0.0
23	5.05539996157896E-4	0.0	0.0	12.842882502394094	0.0
24	5.77759995609024E-4	0.0	0.0	13.28963541899877	0.0
25	5.77759995609024E-4	0.0	0.0	13.773509415321328	0.0
26	7.2219999451128E-4	0.0	0.0	14.11799881270321	0.0
27	7.94419993962408E-4	0.0	0.0	14.441616630243713	0.0
28	7.94419993962408E-4	0.0	0.0	14.751007107892345	0.0
29	7.94419993962408E-4	0.0	0.0	15.039670445698505	0.0
30	7.94419993962408E-4	0.0	0.0	15.385315363071603	0.0
31	8.66639993413536E-4	0.0	0.0	15.69246702073725	0.0
32	8.66639993413536E-4	0.0	0.0	16.023017958225065	0.0
33	8.66639993413536E-4	0.0	0.0	16.355229955700253	0.0
34	8.66639993413536E-4	0.0	0.0	16.683975393201788	0.0
35	9.388599928646641E-4	0.0	0.0	17.021242790638556	0.0
36	0.001011079992315792	0.0	0.0	17.347243868160948	0.0
37	0.001011079992315792	0.0	0.0	17.75839232503622	0.0
38	0.0010832999917669201	0.0	0.0	18.11190922234949	0.0
39	0.0010832999917669201	0.0	0.0	18.48600881950633	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGCA	40	6.8175723E-9	45.000004	15
TCGCCAC	20	7.0337864E-4	45.000004	18
AACCGTA	35	1.2121745E-7	45.000004	20
CGGCGAA	40	6.8175723E-9	45.000004	39
CCTTAGT	20	7.0337864E-4	45.000004	21
CTGTCGT	20	7.0337864E-4	45.000004	39
TCGTTCG	35	1.2121745E-7	45.000004	3
CTCACCG	40	6.8175723E-9	45.000004	21
GTCGCAT	20	7.0337864E-4	45.000004	44
ACCGGTG	35	1.2121745E-7	45.000004	22
TCGCACA	75	0.0	45.000004	26
AAATCCG	20	7.0337864E-4	45.000004	21
GTCGACT	20	7.0337864E-4	45.000004	37
CTCCGAC	35	1.2121745E-7	45.000004	21
GGTCGAC	20	7.0337864E-4	45.000004	11
ACGCATA	30	2.1658252E-6	45.000004	26
CCCTATT	40	6.8175723E-9	45.000004	31
TCCGACA	20	7.0337864E-4	45.000004	20
CGGCCAG	40	6.8175723E-9	45.000004	24
TAGCGCA	40	6.8175723E-9	45.000004	3
>>END_MODULE