FastQCFastQC Report
Thu 26 May 2016
SRR1545613_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545613_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1145506
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCTT58650.512000810122339Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT49690.4337821015341692Illumina Single End Adapter 1 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC44980.3926649009258791No Hit
TTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC44940.392315710262539No Hit
TGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC43880.38306215768402785No Hit
GCCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC36740.32073162427783003No Hit
GGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC32950.28764580892636094No Hit
ATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC30440.2657340948017732No Hit
AGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC25510.2226963455451128No Hit
ACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC17900.15626282184466952No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT15760.1375811213559772Illumina Single End Adapter 1 (95% over 21bp)
CTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC14980.13077190342084632No Hit
TGGTGGGTTGGTGAGACAATCAGATCGATAAATTGATTAAATGCTCCTAACC14370.12544674580491066No Hit
TCCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC14220.1241372808173855No Hit
TTGCTTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAG12430.10851099863291855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGA551.8189894E-1246.00000446
CAGCGTT351.01956175E-746.00000432
GTACCCA351.01956175E-746.00000431
CGAACGC351.01956175E-746.00000431
TCACCCG351.01956175E-746.00000442
GGTCGTA551.8189894E-1246.00000440
CTACTAT351.01956175E-746.00000425
GATCGCA351.01956175E-746.00000438
TTAGCTA750.046.00000433
TTAGCGT351.01956175E-746.00000426
TTAGCAA351.01956175E-746.00000442
TAGACGG750.046.0000042
TCCGAAC351.01956175E-746.00000429
CGTGAAA351.01956175E-746.00000426
GCAAATC351.01956175E-746.00000426
TAAACCG351.01956175E-746.00000425
CGCGATA351.01956175E-746.00000432
CATTAGG351.01956175E-746.00000416
AGTATAC351.01956175E-746.00000428
CATTAAG351.01956175E-746.00000435
Produced by FastQC (version 0.11.5)