##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545613_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1145506
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56975781881544	33.0	31.0	34.0	31.0	34.0
2	32.95746159339192	34.0	31.0	34.0	31.0	34.0
3	33.02517140896687	34.0	33.0	34.0	31.0	34.0
4	36.40062557507338	37.0	37.0	37.0	35.0	37.0
5	36.37218312256767	37.0	37.0	37.0	35.0	37.0
6	36.37725162504605	37.0	37.0	37.0	35.0	37.0
7	36.47922839339122	37.0	37.0	37.0	35.0	37.0
8	36.53281082770409	37.0	37.0	37.0	35.0	37.0
9	38.35937131712972	39.0	39.0	39.0	37.0	39.0
10	38.10571834630286	39.0	39.0	39.0	35.0	39.0
11	37.90326545648823	39.0	38.0	39.0	35.0	39.0
12	37.31328338742879	39.0	35.0	39.0	35.0	39.0
13	37.1692823957273	39.0	35.0	39.0	35.0	39.0
14	38.4470888847374	40.0	36.0	41.0	35.0	41.0
15	38.53071830265402	40.0	36.0	41.0	35.0	41.0
16	38.62359428933589	40.0	36.0	41.0	35.0	41.0
17	38.56352040059153	40.0	36.0	41.0	35.0	41.0
18	38.58490920169776	40.0	36.0	41.0	35.0	41.0
19	38.572796650563156	40.0	36.0	41.0	35.0	41.0
20	38.431392764420266	40.0	36.0	41.0	35.0	41.0
21	38.3982816327457	40.0	35.0	41.0	35.0	41.0
22	38.484816316981316	40.0	35.0	41.0	35.0	41.0
23	38.508526363022106	40.0	35.0	41.0	35.0	41.0
24	38.473796732622965	40.0	35.0	41.0	35.0	41.0
25	38.427041848755046	40.0	35.0	41.0	35.0	41.0
26	38.29345372263436	40.0	35.0	41.0	35.0	41.0
27	38.181860243420815	40.0	35.0	41.0	35.0	41.0
28	38.16599476563196	40.0	35.0	41.0	35.0	41.0
29	38.11047519611421	40.0	36.0	41.0	35.0	41.0
30	38.09952719584184	40.0	36.0	41.0	35.0	41.0
31	38.03809320946377	40.0	35.0	41.0	34.0	41.0
32	37.922421183302404	40.0	35.0	41.0	34.0	41.0
33	37.861022115990664	40.0	35.0	41.0	34.0	41.0
34	37.754519836648605	40.0	35.0	41.0	34.0	41.0
35	37.69328227001866	40.0	35.0	41.0	34.0	41.0
36	37.59890389050778	40.0	35.0	41.0	34.0	41.0
37	37.514146586748566	40.0	35.0	41.0	33.0	41.0
38	37.44840707949151	40.0	35.0	41.0	33.0	41.0
39	37.42561016703535	39.0	35.0	41.0	33.0	41.0
40	37.28907137980945	39.0	35.0	41.0	33.0	41.0
41	37.195329400282496	39.0	35.0	41.0	33.0	41.0
42	37.13394691952727	39.0	35.0	41.0	33.0	41.0
43	37.04165757315981	39.0	35.0	41.0	33.0	41.0
44	36.92406849025671	39.0	35.0	41.0	33.0	41.0
45	36.813430920484045	38.0	35.0	41.0	32.0	41.0
46	36.81353480470639	38.0	35.0	41.0	33.0	41.0
47	36.777723556227556	38.0	35.0	41.0	33.0	41.0
48	36.691880269505354	38.0	35.0	40.0	33.0	41.0
49	36.62748078141887	38.0	35.0	40.0	33.0	41.0
50	36.446842705319746	37.0	35.0	40.0	32.0	41.0
51	36.236327876065246	37.0	35.0	40.0	32.0	41.0
52	35.86403039355534	36.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	5.0
13	7.0
14	10.0
15	14.0
16	27.0
17	62.0
18	112.0
19	256.0
20	410.0
21	721.0
22	1142.0
23	1782.0
24	2537.0
25	3515.0
26	4492.0
27	5689.0
28	7213.0
29	9233.0
30	11604.0
31	14771.0
32	19097.0
33	27060.0
34	67231.0
35	176867.0
36	63124.0
37	84714.0
38	152697.0
39	490559.0
40	553.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.184313307830777	13.408572281594337	46.118745776975416	12.288368633599475
2	24.733261982041125	12.911150181666443	50.319684052287805	12.035903784004622
3	24.838019181043137	13.859290130300497	45.11115611790772	16.191534570748647
4	22.10446737075144	13.77190516679965	49.232828112641926	14.890799349806985
5	22.462213205343314	16.598167098208126	45.048738286835686	15.89088140961287
6	22.38547855707434	19.06450075337886	45.69814562298233	12.85187506656447
7	84.92151066864774	2.688593512386666	6.893460182661635	5.496435636303956
8	87.44703214125461	2.1252616747533404	7.014105556845621	3.4136006271464314
9	80.06409394625607	3.5540625714749643	8.97612059648749	7.405722885781479
10	46.563876575068136	19.091650327453543	14.720481603762877	19.62399149371544
11	38.437773350816144	20.26650231426112	24.312923721045546	16.982800613877185
12	32.032394417838056	23.98372422318172	24.712921625901565	19.27095973307866
13	19.662227871351178	32.50921426862888	30.86417705363394	16.964380806385996
14	14.401932421130923	40.08621517477866	29.233107465172598	16.278744938917825
15	14.156102194139534	24.43007718859613	42.647092202048704	18.766728415215635
16	18.70885006276702	21.82223401710685	39.27356120352054	20.19535471660559
17	18.234387248953738	22.06317557481148	27.599942732731215	32.10249444350357
18	22.042660623340254	22.754660385890602	31.52196496570075	23.680714025068397
19	25.983102663800977	24.506811836865104	28.657292061324863	20.852793438009055
20	27.92984061192172	21.109099384900645	25.766342559532646	25.194717443644993
21	22.803023292763196	27.155946804294345	29.47518389253308	20.565846010409373
22	25.889781459023347	21.473392544430148	25.37001115664169	27.266814839904814
23	22.92777165724143	27.440799087913987	21.90150029768504	27.729928957159544
24	22.90987563574525	21.012024380492115	31.729820708053907	24.348279275708727
25	26.984406890928554	21.183826186855413	28.342845868987155	23.48892105322888
26	20.941662461829097	29.290811222289538	25.225882710348092	24.541643605533274
27	22.476268129542753	28.414953740966876	23.87818134518719	25.230596784303184
28	22.17709902872617	25.650149366306245	31.47639558413487	20.696356020832717
29	18.973100097249603	26.61889156407736	29.170689634100565	25.237318704572477
30	23.34976857388787	27.348088966797206	29.86784879345896	19.43429366585596
31	27.568428275364777	27.413387620841796	22.390629119358607	22.627554984434823
32	27.11072661339181	27.52154942881137	22.731002718449314	22.6367212393475
33	28.281999395900154	24.573681848894722	24.85844683484853	22.28587192035659
34	21.20215869668077	26.783098473513018	27.28418707540598	24.73055575440024
35	22.85129890196996	24.157010089864215	28.649347973733878	24.342343034431945
36	30.138296962215826	24.158843340846754	24.078616785944376	21.624242910993047
37	23.54173614105906	26.34137228438786	29.742052856990707	20.37483871756237
38	20.96872473823795	26.708808159887422	29.690547234148053	22.631919867726577
39	21.17745345724946	28.53909102178426	25.46280857542431	24.82064694554197
40	26.620113731399048	22.407652164196435	26.621335898720737	24.350898205683777
41	22.688139564524324	22.375788516166654	27.48776523213322	27.4483066871758
42	23.43793921638123	24.29101200692096	25.34155211757948	26.92949665911833
43	22.082904847290195	22.72655053749173	28.053977892739102	27.13656672247897
44	25.950889825107858	25.566605500102142	25.2194226830763	23.263081991713705
45	19.841013490981275	28.184313307830777	25.112483042428412	26.862190158759535
46	22.372122014201583	25.945128179162747	25.41173944091083	26.27101036572484
47	23.376132468970045	24.641686730580197	29.461128968333643	22.52105183211611
48	23.460636609498337	20.43498680932269	33.669662140573685	22.434714440605287
49	21.36863534542813	21.162787449389178	28.03774052689379	29.430836678288898
50	19.98007867265645	24.764689141741727	31.048375128545814	24.20685705705601
51	23.558933781228557	24.955783732254567	26.147571466234137	25.33771102028274
52	21.49303451924302	21.496701021208096	30.325637753097755	26.684626706451127
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	546.0
1	420.0
2	294.0
3	240.5
4	187.0
5	208.0
6	229.0
7	278.5
8	328.0
9	487.0
10	646.0
11	709.5
12	773.0
13	728.5
14	756.0
15	828.0
16	870.5
17	913.0
18	891.0
19	869.0
20	1279.5
21	1690.0
22	2393.0
23	3096.0
24	3030.5
25	2965.0
26	4144.5
27	5324.0
28	6009.5
29	6695.0
30	8197.0
31	9699.0
32	10933.5
33	12168.0
34	17337.5
35	22507.0
36	25071.5
37	27636.0
38	30992.5
39	44957.0
40	55565.0
41	71109.5
42	86654.0
43	91433.5
44	96213.0
45	100824.0
46	105435.0
47	117341.5
48	129248.0
49	130261.5
50	131275.0
51	112322.0
52	93369.0
53	79648.5
54	65928.0
55	60215.5
56	54503.0
57	50430.5
58	46358.0
59	42738.0
60	39118.0
61	37280.5
62	35443.0
63	30191.0
64	22176.0
65	19413.0
66	14509.0
67	9605.0
68	8729.0
69	7853.0
70	6275.5
71	4698.0
72	4054.0
73	3410.0
74	2691.5
75	1973.0
76	1584.5
77	1196.0
78	809.5
79	423.0
80	377.0
81	331.0
82	206.5
83	82.0
84	49.5
85	17.0
86	19.0
87	21.0
88	14.0
89	4.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1145506.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.793918944262243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.77999740916755	9.731439498250051
2	12.263145383399205	3.628399576074232
3	5.864111961003664	2.6025959109350074
4	3.3787987905118326	1.999427017432134
5	2.220364125769941	1.6423943451694103
6	1.5602696300252457	1.384950146267251
7	1.1435571107685174	1.1842383843390916
8	0.8539562214252626	1.0106687297371035
9	0.6232034247595222	0.8297658856511104
>10	3.9508868463316227	11.3755607930491
>50	0.6358901743235337	6.825582593494943
>100	1.700430549723094	52.06888034188658
>500	0.014760681855235193	1.4344178922024207
>1k	0.010037263661559931	3.769386781153529
>5k	5.904272742094077E-4	0.5122921043580073
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCTT	5865	0.512000810122339	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	4969	0.4337821015341692	Illumina Single End Adapter 1 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	4498	0.3926649009258791	No Hit
TTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	4494	0.392315710262539	No Hit
TGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	4388	0.38306215768402785	No Hit
GCCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	3674	0.32073162427783003	No Hit
GGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	3295	0.28764580892636094	No Hit
ATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	3044	0.2657340948017732	No Hit
AGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	2551	0.2226963455451128	No Hit
ACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	1790	0.15626282184466952	No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	1576	0.1375811213559772	Illumina Single End Adapter 1 (95% over 21bp)
CTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	1498	0.13077190342084632	No Hit
TGGTGGGTTGGTGAGACAATCAGATCGATAAATTGATTAAATGCTCCTAACC	1437	0.12544674580491066	No Hit
TCCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	1422	0.1241372808173855	No Hit
TTGCTTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAG	1243	0.10851099863291855	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5343490125761018	0.0
2	0.0	0.0	0.0	1.2686096799143785	0.0
3	0.0	0.0	0.0	4.40015154874789	0.0
4	0.0	0.0	0.0	5.054796744844636	0.0
5	0.0	0.0	0.0	6.078187281428469	0.0
6	0.0	0.0	0.0	9.400649145443149	0.0
7	0.0	0.0	0.0	9.831637721670598	0.0
8	0.0	0.0	0.0	10.685059702873664	0.0
9	0.0	0.0	0.0	11.20936948387874	0.0
10	0.0	0.0	0.0	12.950084940628857	0.0
11	0.0	0.0	0.0	14.394861310198287	0.0
12	0.0	0.0	0.0	15.009611473008436	0.0
13	0.0	0.0	0.0	15.296384305276446	0.0
14	0.0	0.0	0.0	15.439552477245863	0.0
15	0.0	0.0	0.0	15.666351813085221	0.0
16	0.0	0.0	0.0	16.29262526778559	0.0
17	0.0	0.0	0.0	17.183497947631878	0.0
18	0.0	0.0	0.0	18.21841177610593	0.0
19	8.729766583501091E-5	0.0	0.0	18.755379718657082	0.0
20	8.729766583501091E-5	0.0	0.0	19.536082744219584	0.0
21	8.729766583501091E-5	0.0	0.0	20.42206675477911	0.0
22	8.729766583501091E-5	0.0	0.0	21.11983699779835	0.0
23	8.729766583501091E-5	0.0	0.0	21.907349241295986	0.0
24	8.729766583501091E-5	0.0	0.0	22.440214193552894	0.0
25	8.729766583501091E-5	0.0	0.0	22.905947240782677	0.0
26	8.729766583501091E-5	0.0	0.0	23.358760233468878	0.0
27	8.729766583501091E-5	0.0	0.0	23.77499550417021	0.0
28	8.729766583501091E-5	0.0	0.0	24.260981609873713	0.0
29	8.729766583501091E-5	0.0	0.0	24.690573423447805	0.0
30	8.729766583501091E-5	0.0	0.0	25.15770323333095	0.0
31	8.729766583501091E-5	0.0	0.0	25.620380862256503	0.0
32	8.729766583501091E-5	0.0	0.0	26.06123407472331	0.0
33	8.729766583501091E-5	0.0	0.0	26.504182431170154	0.0
34	1.7459533167002181E-4	0.0	0.0	26.958130293512212	0.0
35	1.7459533167002181E-4	0.0	0.0	27.491606329429963	0.0
36	1.7459533167002181E-4	0.0	0.0	27.93438009054514	0.0
37	1.7459533167002181E-4	0.0	0.0	28.406573165046712	0.0
38	1.7459533167002181E-4	0.0	0.0	28.835030108964947	0.0
39	1.7459533167002181E-4	0.0	0.0	29.266891661850746	0.0
40	1.7459533167002181E-4	0.0	0.0	29.70652270699586	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGA	55	1.8189894E-12	46.000004	46
CAGCGTT	35	1.01956175E-7	46.000004	32
GTACCCA	35	1.01956175E-7	46.000004	31
CGAACGC	35	1.01956175E-7	46.000004	31
TCACCCG	35	1.01956175E-7	46.000004	42
GGTCGTA	55	1.8189894E-12	46.000004	40
CTACTAT	35	1.01956175E-7	46.000004	25
GATCGCA	35	1.01956175E-7	46.000004	38
TTAGCTA	75	0.0	46.000004	33
TTAGCGT	35	1.01956175E-7	46.000004	26
TTAGCAA	35	1.01956175E-7	46.000004	42
TAGACGG	75	0.0	46.000004	2
TCCGAAC	35	1.01956175E-7	46.000004	29
CGTGAAA	35	1.01956175E-7	46.000004	26
GCAAATC	35	1.01956175E-7	46.000004	26
TAAACCG	35	1.01956175E-7	46.000004	25
CGCGATA	35	1.01956175E-7	46.000004	32
CATTAGG	35	1.01956175E-7	46.000004	16
AGTATAC	35	1.01956175E-7	46.000004	28
CATTAAG	35	1.01956175E-7	46.000004	35
>>END_MODULE