##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545612_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1389465
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.919820218573335	31.0	31.0	34.0	31.0	34.0
2	32.58364766294941	34.0	31.0	34.0	31.0	34.0
3	32.55180878971402	34.0	31.0	34.0	31.0	34.0
4	36.02692043340422	37.0	35.0	37.0	35.0	37.0
5	35.804077108815264	37.0	35.0	37.0	35.0	37.0
6	35.81122518379376	37.0	35.0	37.0	35.0	37.0
7	35.800624700874074	37.0	35.0	37.0	35.0	37.0
8	35.77183592245937	37.0	35.0	37.0	33.0	37.0
9	37.946976714059005	39.0	38.0	39.0	35.0	39.0
10	37.88334502848218	39.0	38.0	39.0	35.0	39.0
11	37.72540006405343	39.0	38.0	39.0	35.0	39.0
12	37.27120222531694	39.0	37.0	39.0	34.0	39.0
13	37.0035214992821	39.0	37.0	39.0	33.0	39.0
14	37.658251917104785	40.0	35.0	41.0	33.0	41.0
15	37.51220577704368	40.0	35.0	41.0	33.0	41.0
16	37.560165243457014	40.0	35.0	41.0	33.0	41.0
17	37.65074830960118	40.0	35.0	41.0	33.0	41.0
18	37.72881144901095	40.0	35.0	41.0	33.0	41.0
19	37.6850629558859	40.0	35.0	41.0	33.0	41.0
20	37.627072290413935	40.0	35.0	41.0	33.0	41.0
21	37.61406512578582	39.0	35.0	41.0	33.0	41.0
22	37.52593480224403	39.0	35.0	41.0	33.0	41.0
23	37.454797350059195	39.0	35.0	41.0	32.0	41.0
24	37.41533683827948	39.0	35.0	41.0	32.0	41.0
25	37.3409341005351	39.0	35.0	41.0	32.0	41.0
26	37.256849218943984	39.0	35.0	41.0	32.0	41.0
27	37.167093809487824	39.0	35.0	41.0	32.0	41.0
28	37.06253845904719	39.0	35.0	41.0	32.0	41.0
29	36.977035765564445	39.0	35.0	41.0	31.0	41.0
30	36.94502920188706	39.0	35.0	41.0	31.0	41.0
31	36.86626075503881	39.0	35.0	41.0	31.0	41.0
32	36.7238390315697	39.0	35.0	41.0	31.0	41.0
33	36.62578762329386	39.0	35.0	41.0	31.0	41.0
34	36.52543676882829	39.0	35.0	41.0	31.0	41.0
35	36.43062977476943	38.0	35.0	41.0	30.0	41.0
36	36.341814295430254	38.0	35.0	41.0	30.0	41.0
37	36.18257674716528	38.0	35.0	41.0	30.0	41.0
38	36.10925284192117	38.0	35.0	40.0	30.0	41.0
39	36.048363218936785	38.0	35.0	40.0	30.0	41.0
40	35.94544303023106	38.0	35.0	40.0	30.0	41.0
41	35.907712680780016	38.0	35.0	40.0	30.0	41.0
42	35.76568823252115	38.0	35.0	40.0	29.0	41.0
43	35.65915370304398	38.0	35.0	40.0	29.0	41.0
44	35.5615369944547	37.0	34.0	40.0	29.0	41.0
45	35.518264943701354	37.0	34.0	40.0	29.0	41.0
46	35.47743987793863	37.0	34.0	40.0	29.0	41.0
47	35.4075619033225	37.0	34.0	40.0	29.0	41.0
48	35.361053355068314	37.0	34.0	40.0	29.0	41.0
49	35.32398369156474	37.0	34.0	40.0	29.0	41.0
50	35.22176161328281	36.0	34.0	40.0	28.0	41.0
51	35.02604815522521	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	16.0
11	14.0
12	26.0
13	14.0
14	38.0
15	37.0
16	87.0
17	164.0
18	379.0
19	730.0
20	1247.0
21	2031.0
22	3179.0
23	4676.0
24	7009.0
25	9989.0
26	13327.0
27	16449.0
28	18974.0
29	22297.0
30	27279.0
31	33603.0
32	42040.0
33	54924.0
34	90438.0
35	177082.0
36	102182.0
37	140876.0
38	212699.0
39	407477.0
40	174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.013868647285106	91.59791718395209	3.112492937929347	3.2757212308334505
2	3.694515514964393	2.456916870881958	91.1691190494183	2.6794485647353476
3	27.233935363611174	15.364906636727085	44.12288182861749	13.27827617104425
4	27.158582619929255	15.376277919918818	43.59627626460544	13.868863195546488
5	23.69336399261586	14.344297985195741	47.63178633502823	14.330551687160165
6	23.262550693972138	14.158111215467825	47.563774546318186	15.015563544241846
7	22.02106566196342	17.074269592972836	45.37199569618522	15.532669048878525
8	25.109520570867204	19.199907878212116	43.5879277275786	12.102643823342078
9	82.4107120366472	3.860910494326953	9.49207068907817	4.236306779947677
10	83.65097357616061	4.425660236134052	8.504640275213841	3.4187259124914986
11	76.70333545645266	6.2806907694688245	10.710957094996996	6.3050166790815165
12	44.662154138463364	22.18062347738158	16.143911505507518	17.013310878647538
13	35.19311389635579	22.735009518051914	23.5167492524101	18.555127333182195
14	29.774985336082594	22.3348555019378	28.15975933182916	19.730399830150454
15	21.822715937429155	28.125717452400746	30.36319734574099	19.688369264429113
16	17.71566754110395	33.00565325502981	29.10868571716452	20.169993486701713
17	17.310187734127883	22.50110654100679	40.7288416764726	19.459864048392728
18	21.657688390855473	19.796612365190917	36.709524889076015	21.836174354877596
19	20.872709999892045	19.97732940376332	29.03873073449133	30.111229861853307
20	22.57689110556941	21.872591249149853	32.51301760029939	23.03750004498134
21	26.714886665011356	24.281000241099992	27.684468482473466	21.319644611415185
22	28.901123813842016	20.707466542878013	26.543237864933626	23.84817177834634
23	25.00775478331588	24.930170965083683	27.058328205460374	23.003746046140062
24	28.48031436560115	21.73383280615201	24.57118387292951	25.214668955317332
25	26.859114839164715	24.280784330659642	23.886675806875306	24.973425023300337
26	26.805712990251646	20.106875667972925	30.446826656302967	22.64058468547247
27	27.18413202203726	22.20300619303113	27.663453199612803	22.94940858531881
28	24.03874872702803	27.229833065244534	24.72692727056817	24.004490937159268
29	21.95499706721652	25.898241409463356	27.72254069012174	24.42422083319839
30	22.77819160612178	24.729374255558795	29.641696624240264	22.85073751407916
31	23.163231891411442	23.179065323703725	29.210379534569057	24.44732325031577
32	23.26557344013703	25.618781329504518	29.028942794528827	22.086702435829615
33	27.21191249869554	25.85606690344845	24.071063322933647	22.86095727492236
34	27.99322041217303	24.414001072355187	26.231895009949874	21.36088350552191
35	27.85302256623952	26.275149068166527	23.1825918608961	22.689236504697853
36	24.947875621192328	24.474240085212653	25.747320011659163	24.830564281935853
37	24.84618180378779	23.5397796993807	29.07334837509401	22.5406901217375
38	28.780933668714216	25.362135786075935	24.118851500397636	21.73807904481221
39	27.096256472815078	27.382481746571525	25.3201052203546	20.201156560258802
40	24.616021274375388	25.58589097242464	26.220883577492053	23.577204175707916
41	22.75998315898565	27.136055963986138	25.374298740882285	24.729662136145926
42	25.573080286297245	23.92712302936742	27.234583094932223	23.265213589403118
43	23.157906100549493	23.214258725480672	28.474772664298847	25.15306250967099
44	23.925179835404276	24.24976519739612	25.729615355550518	26.09543961164909
45	23.716322469439678	22.726517040731505	27.132673367087335	26.424487122741485
46	23.670477485938832	24.560028500178127	27.384640850975018	24.384853162908026
47	20.819308150978973	27.71030576516861	26.15654226626795	25.313843817584463
48	22.616474686300123	25.745952578870284	26.41880148114562	25.21877125368397
49	23.845580853062152	25.2212182386746	27.970693756229913	22.962507152033336
50	24.327780836509017	23.390945435833217	28.30441932686322	23.97685440079455
51	22.22330177442397	23.566696534277582	27.820276149453203	26.389725541845245
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	665.0
1	915.5
2	1166.0
3	3420.5
4	5675.0
5	3989.5
6	2304.0
7	2533.5
8	2763.0
9	2803.5
10	2844.0
11	3064.5
12	3285.0
13	3142.5
14	3000.0
15	3541.5
16	4083.0
17	3382.5
18	2682.0
19	3286.5
20	3891.0
21	4255.5
22	4620.0
23	5621.5
24	6623.0
25	6549.0
26	9035.5
27	11596.0
28	13357.0
29	15118.0
30	18512.5
31	21907.0
32	28526.0
33	35145.0
34	39112.5
35	43080.0
36	43872.5
37	44665.0
38	51916.5
39	59168.0
40	68084.0
41	77000.0
42	84168.0
43	91336.0
44	97185.0
45	103034.0
46	112692.0
47	122350.0
48	126746.0
49	131142.0
50	120833.5
51	110525.0
52	98634.5
53	86744.0
54	80627.5
55	74511.0
56	75157.5
57	75804.0
58	71863.0
59	67922.0
60	62614.0
61	57306.0
62	48031.5
63	38757.0
64	32386.5
65	26016.0
66	21477.5
67	16939.0
68	14709.0
69	12479.0
70	10066.0
71	7653.0
72	5582.5
73	3512.0
74	3390.5
75	2271.0
76	1273.0
77	1014.5
78	756.0
79	473.0
80	190.0
81	127.0
82	64.0
83	71.0
84	78.0
85	51.5
86	25.0
87	17.0
88	9.0
89	8.5
90	8.0
91	7.0
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1389465.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.974444838743326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.27166241799816	11.70474129539334
2	11.08159980969237	3.5404480976991866
3	4.5385206255594746	2.1750104214749606
4	2.3914096204530764	1.5280576427507113
5	1.3617639219093887	1.0876711326966153
6	0.8598097168157636	0.8240989735853361
7	0.5757200641130034	0.643776588471166
8	0.4345249559864138	0.5553035952369871
9	0.289277025511783	0.4158935898438351
>10	2.3926224792506314	8.40871993746973
>50	0.9147874229841834	10.885298287289617
>100	1.8729425459577242	55.35755207847167
>500	0.009486684386182396	1.0563374435700215
>1k	0.005872709381922435	1.8170909160468054
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	4441	0.31961942186381087	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3307	0.2380052754117592	No Hit
ATCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG	2620	0.18856178457175962	No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCT	2022	0.14552363679545724	Illumina Single End Adapter 2 (95% over 21bp)
ATGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCT	1908	0.1373190400621822	No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	1669	0.12011817498101787	No Hit
ATGCCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTC	1578	0.11356889162375447	No Hit
ATTGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTC	1492	0.10737945900040663	No Hit
ATGTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTC	1456	0.10478853371621451	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15775856174858668	0.0
2	0.0	0.0	0.0	0.6478752613415955	0.0
3	0.0	0.0	0.0	0.9279830726214766	0.0
4	0.0	0.0	0.0	1.2117613613872966	0.0
5	0.0	0.0	0.0	2.238559445541989	0.0
6	0.0	0.0	0.0	2.4708790793578825	0.0
7	0.0	0.0	0.0	2.8605974241884464	0.0
8	0.0	0.0	0.0	4.081570964363982	0.0
9	0.0	0.0	0.0	4.232924183048872	0.0
10	0.0	0.0	0.0	4.535918501005783	0.0
11	0.0	0.0	0.0	4.775507119646771	0.0
12	7.197014678311437E-5	0.0	0.0	5.43180288816199	0.0
13	7.197014678311437E-5	0.0	0.0	6.019727017233252	0.0
14	7.197014678311437E-5	0.0	0.0	6.2760127099279215	0.0
15	7.197014678311437E-5	0.0	0.0	6.42225604819121	0.0
16	7.197014678311437E-5	0.0	0.0	6.516824821064223	0.0
17	7.197014678311437E-5	0.0	0.0	6.644140010723552	0.0
18	7.197014678311437E-5	0.0	0.0	6.932452418736708	0.0
19	7.197014678311437E-5	0.0	0.0	7.29878046586276	0.0
20	1.4394029356622873E-4	0.0	0.0	7.732688480818156	0.0
21	2.159104403493431E-4	0.0	0.0	7.986455218375418	0.0
22	2.159104403493431E-4	0.0	0.0	8.27282443242543	0.0
23	2.8788058713245747E-4	0.0	0.0	8.716664327636897	0.0
24	3.598507339155718E-4	0.0	0.0	9.081337061386936	0.0
25	3.598507339155718E-4	0.0	0.0	9.465441734768419	0.0
26	0.0012234924953129442	0.0	0.0	9.759367814230657	0.0
27	0.0012234924953129442	0.0	0.0	10.041634729914032	0.0
28	0.0012954626420960587	0.0	0.0	10.293602213801716	0.0
29	0.0012954626420960587	0.0	0.0	10.531895369800607	0.0
30	0.0012954626420960587	0.0	0.0	10.78271133133976	0.0
31	0.0012954626420960587	0.0	0.0	11.045906158125609	0.0
32	0.0012954626420960587	0.0	0.0	11.338752685386101	0.0
33	0.0012954626420960587	0.0	0.0	11.614254407271863	0.0
34	0.0012954626420960587	0.0	0.0	11.912210814953957	0.0
35	0.0012954626420960587	0.0	0.0	12.206136894416197	0.0
36	0.0012954626420960587	0.0	0.0	12.485237123641113	0.0
37	0.0012954626420960587	0.0	0.0	12.811261888568621	0.0
38	0.001367432788879173	0.0	0.0	13.116847131809726	0.0
39	0.001367432788879173	0.0	0.0	13.409189868042736	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACTTA	20	7.033795E-4	45.000004	11
ATCTCGA	35	1.2121745E-7	45.000004	1
ACACGTT	40	6.8175723E-9	45.000004	19
ACACGTA	20	7.033795E-4	45.000004	39
ACACGGT	20	7.033795E-4	45.000004	32
CGAACGA	20	7.033795E-4	45.000004	25
CGAAATA	35	1.2121745E-7	45.000004	45
CGACGAA	40	6.8175723E-9	45.000004	23
CCGATCA	20	7.033795E-4	45.000004	32
ACGTTAG	80	0.0	45.000004	3
CGCATTA	35	1.2121745E-7	45.000004	3
CCGGATG	20	7.033795E-4	45.000004	25
TCGATTA	20	7.033795E-4	45.000004	38
CACGAAC	35	1.2121745E-7	45.000004	45
ATCCGTT	80	0.0	45.000004	1
ACGATTT	40	6.8175723E-9	45.000004	14
GCCGATT	20	7.033795E-4	45.000004	18
TACGCTA	35	1.2121745E-7	45.000004	2
CCCGTAG	20	7.033795E-4	45.000004	17
TACGACA	35	1.2121745E-7	45.000004	2
>>END_MODULE