FastQCFastQC Report
Thu 26 May 2016
SRR1545611_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545611_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1142506
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCTT46760.40927574997417954Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCT29600.25907960220777837Illumina Single End Adapter 2 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC28830.2523400314746706No Hit
TGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC28440.24892648266179782No Hit
GCCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC25760.22546927543487735No Hit
TTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC22830.1998238958920128No Hit
GGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC18950.1658634615485608No Hit
ATCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC16900.1479204485578194No Hit
ACCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC13760.12043700426956183No Hit
AGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC13190.11544797138920934No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC301.8613937E-646.042
AACGTAC253.417144E-546.028
CGTGGCA253.417144E-546.031
AACCGGT206.3114386E-446.018
ACTATCG206.3114386E-446.023
GGTACTC206.3114386E-446.010
AGCCGAC301.8613937E-646.029
GGTACGC253.417144E-546.09
TACCCGC405.6115823E-946.024
GCCCACG301.8613937E-646.039
CGAACAA206.3114386E-446.039
CTCCGTA301.8613937E-646.010
ATCTACG301.8613937E-646.046
TACCACG405.6115823E-946.045
ACCGCGA206.3114386E-446.012
GACCGTA206.3114386E-446.045
GGCGTAA301.8613937E-646.031
ACCGAAC206.3114386E-446.012
ATTCGCG301.8613937E-646.01
AAGCGTG253.417144E-546.032
Produced by FastQC (version 0.11.5)