##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545611_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1142506
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.574191295275476	33.0	31.0	34.0	31.0	34.0
2	32.988999620133285	34.0	31.0	34.0	31.0	34.0
3	33.059781742940515	34.0	33.0	34.0	31.0	34.0
4	36.43266556149377	37.0	37.0	37.0	35.0	37.0
5	36.4115593266031	37.0	37.0	37.0	35.0	37.0
6	36.42909971588771	37.0	37.0	37.0	35.0	37.0
7	36.50523585871759	37.0	37.0	37.0	35.0	37.0
8	36.538314021983254	37.0	37.0	37.0	35.0	37.0
9	38.358364857602496	39.0	39.0	39.0	37.0	39.0
10	38.12837919450751	39.0	39.0	39.0	37.0	39.0
11	37.923047231261805	39.0	38.0	39.0	35.0	39.0
12	37.51413734369885	39.0	37.0	39.0	35.0	39.0
13	37.41511204317526	39.0	37.0	39.0	35.0	39.0
14	38.73652479724395	41.0	38.0	41.0	35.0	41.0
15	38.77304889427276	41.0	38.0	41.0	35.0	41.0
16	38.84935396400544	41.0	38.0	41.0	35.0	41.0
17	38.79587240679699	40.0	38.0	41.0	35.0	41.0
18	38.78051318767692	40.0	37.0	41.0	35.0	41.0
19	38.76836708078557	40.0	38.0	41.0	35.0	41.0
20	38.67121398049551	40.0	37.0	41.0	35.0	41.0
21	38.62595732538823	40.0	37.0	41.0	35.0	41.0
22	38.71782380136297	40.0	37.0	41.0	35.0	41.0
23	38.715640005391656	40.0	37.0	41.0	35.0	41.0
24	38.678196875990146	40.0	37.0	41.0	35.0	41.0
25	38.60833816190024	40.0	37.0	41.0	35.0	41.0
26	38.48428892277152	40.0	36.0	41.0	35.0	41.0
27	38.3894666636324	40.0	36.0	41.0	35.0	41.0
28	38.362374464554236	40.0	36.0	41.0	35.0	41.0
29	38.314681060755916	40.0	36.0	41.0	35.0	41.0
30	38.23454931527712	40.0	36.0	41.0	35.0	41.0
31	38.18308525294397	40.0	36.0	41.0	34.0	41.0
32	38.12822514717647	40.0	35.0	41.0	34.0	41.0
33	38.0368024325474	40.0	35.0	41.0	34.0	41.0
34	37.93166600437985	40.0	35.0	41.0	34.0	41.0
35	37.80570867899162	40.0	35.0	41.0	34.0	41.0
36	37.74754880937168	40.0	35.0	41.0	34.0	41.0
37	37.66379520107553	40.0	35.0	41.0	34.0	41.0
38	37.54339058175624	40.0	35.0	41.0	33.0	41.0
39	37.50002363226101	40.0	35.0	41.0	33.0	41.0
40	37.37247856904034	39.0	35.0	41.0	33.0	41.0
41	37.259194262437134	39.0	35.0	41.0	33.0	41.0
42	37.13749774618251	39.0	35.0	41.0	33.0	41.0
43	37.03850045426457	39.0	35.0	41.0	33.0	41.0
44	36.9474225955925	39.0	35.0	41.0	33.0	41.0
45	36.90816765951339	39.0	35.0	41.0	33.0	41.0
46	36.901150628530615	39.0	35.0	41.0	33.0	41.0
47	36.879568247344	38.0	35.0	41.0	33.0	41.0
48	36.76742966776542	38.0	35.0	41.0	33.0	41.0
49	36.68647779530261	38.0	35.0	40.0	33.0	41.0
50	36.58853082609632	38.0	35.0	40.0	33.0	41.0
51	36.461161691929846	38.0	35.0	40.0	32.0	41.0
52	36.095341293612464	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	5.0
13	10.0
14	21.0
15	25.0
16	57.0
17	99.0
18	156.0
19	277.0
20	496.0
21	787.0
22	1200.0
23	1839.0
24	2523.0
25	3270.0
26	4163.0
27	5153.0
28	6651.0
29	8319.0
30	10915.0
31	13853.0
32	18456.0
33	26214.0
34	63097.0
35	140958.0
36	67915.0
37	98368.0
38	170507.0
39	496520.0
40	646.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.78252893201436	12.877656660008787	47.690865518430535	12.648948889546313
2	25.23400314746706	12.92562139717428	49.48315369897401	12.35722175638465
3	25.059824631117912	14.069860464627757	46.1823395238187	14.687975380435638
4	23.742719950704856	13.932093135615917	47.171218356840136	15.15396855683909
5	21.73844163619272	16.724376064545833	45.61385235613643	15.923329943125026
6	24.590067798331035	19.21364089116381	44.03670527769657	12.159586032808582
7	86.88365750376803	2.4641446084309404	6.475151990449065	4.177045897351961
8	87.86483397023736	2.848300140218082	6.208370021689165	3.0784958678553984
9	80.30163517740826	4.484090236725234	8.659123015546527	6.555151570319981
10	45.95030573143599	21.73380271088292	13.705223429898835	18.61066812778226
11	35.524102280425666	21.891526171416167	23.193051064939702	19.39132048321847
12	30.36071582993875	22.341239345788992	26.840821842511108	20.45722298176115
13	22.612835293643972	26.95907067446473	30.771654590873045	19.656439441018254
14	17.8579368510975	33.6977661386461	28.073375544636093	20.370921465620313
15	17.516056808454397	22.00338554020723	39.84644281955631	20.634114831782068
16	21.943254564965088	20.460461476788744	34.81600971898616	22.780274239260013
17	21.015644556790072	20.51035180559227	27.217362534638767	31.25664110297889
18	22.541413349251556	22.082159743581215	30.82819696351704	24.54822994365019
19	26.187696169648124	24.7533929799931	27.21963823384735	21.839272616511423
20	29.41665076594784	20.33932425737808	25.029715380050522	25.214309596623565
21	25.25238379492099	25.56397953271143	26.085027124584027	23.098609547783553
22	28.92011070401381	21.50877106991123	23.493268306687227	26.07784991938773
23	27.53377225152428	24.353482607531163	21.841285735042092	26.271459405902466
24	27.179813497697168	19.25136498189069	28.93927909350148	24.62954242691067
25	28.263396428552674	21.899053484183014	25.609405989990425	24.228144097273887
26	23.707621666757113	27.232679740850372	24.050552032111867	25.009146560280648
27	22.50491463502161	24.293964320537484	27.05491262190308	26.146208422537825
28	24.671730389162068	23.794798451824324	28.330617082098474	23.202854076915134
29	24.669979851309314	22.41686258102802	27.120820372059317	25.79233719560335
30	23.522327235042965	25.66726126602399	28.346809557236462	22.463601941696588
31	27.726856576683183	24.83251729093764	22.9051751150541	24.535451017325073
32	29.41498775498772	23.704033064158963	24.89982547137608	21.981153709477237
33	29.037046632577862	26.210278107948664	22.1931438434459	22.559531416027575
34	25.545948992828045	23.535281215153354	24.884683318949747	26.03408647306885
35	25.2973726177368	22.176863841415276	29.42312775600303	23.102635784844892
36	30.302685500119914	23.243028920635865	23.21003128211143	23.244254297132795
37	28.838448113182775	26.769049790548145	23.822019315434666	20.57048278083441
38	24.54928026636184	24.537289082070465	26.840471734940564	24.072958916627133
39	23.319177317230718	26.427519855475595	25.14209991019741	25.111202917096275
40	26.766336456876378	22.62850260742613	27.412810085898894	23.1923508497986
41	24.267618725853517	21.957784029142953	27.994951448832655	25.779645796170875
42	24.735274913217086	23.654230262248074	24.850722884606295	26.759771939928545
43	24.625866297419883	21.282426525549976	27.16169543092115	26.93001174610899
44	26.455878568690228	23.059485026774475	25.371945530264174	25.11269087427112
45	21.52636397533142	25.64905567235533	25.84091462101731	26.983665731295943
46	22.907800921833232	24.372213362555645	25.97736904663958	26.742616668971543
47	24.973960749440266	23.454231312570787	27.966155101154826	23.605652836834118
48	24.498952303095127	21.72706314014981	29.54015121145972	24.23383334529534
49	22.72863337260373	21.986317796142867	27.012199498295853	28.27284933295755
50	21.83542143323536	23.497294543748566	29.20816170768469	25.459122315331385
51	24.062105581940052	23.654667896711263	26.348308017638423	25.934918503710264
52	23.229199671599098	21.33319212327988	28.148473618519287	27.289134586601733
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	303.0
1	228.0
2	153.0
3	129.0
4	105.0
5	121.0
6	137.0
7	204.5
8	272.0
9	372.5
10	473.0
11	529.5
12	586.0
13	521.5
14	676.0
15	895.0
16	842.5
17	790.0
18	885.0
19	980.0
20	974.0
21	968.0
22	1723.0
23	2478.0
24	2511.5
25	2545.0
26	3801.5
27	5058.0
28	5756.0
29	6454.0
30	7816.5
31	9179.0
32	12732.5
33	16286.0
34	20743.5
35	25201.0
36	27242.5
37	29284.0
38	29601.5
39	34916.5
40	39914.0
41	48747.5
42	57581.0
43	59802.5
44	62024.0
45	68817.5
46	75611.0
47	82038.0
48	88465.0
49	100533.5
50	112602.0
51	112926.0
52	113250.0
53	101636.5
54	90023.0
55	79125.5
56	68228.0
57	66552.0
58	64876.0
59	62832.0
60	60788.0
61	56626.0
62	52464.0
63	46678.5
64	34071.5
65	27250.0
66	23408.0
67	19566.0
68	17033.0
69	14500.0
70	11689.5
71	8879.0
72	7455.5
73	6032.0
74	4343.0
75	2654.0
76	2150.0
77	1646.0
78	1621.0
79	1596.0
80	1169.5
81	743.0
82	524.0
83	305.0
84	169.5
85	34.0
86	28.5
87	23.0
88	16.5
89	8.5
90	7.0
91	4.5
92	2.0
93	4.5
94	7.0
95	5.0
96	3.0
97	2.5
98	2.0
99	3.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1142506.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.241631490172086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.82217906769193	11.177844707680876
2	12.278170562358158	3.9883504329460036
3	5.246033942877926	2.5561245025547326
4	2.9081740561928116	1.8893396531985063
5	1.7434254402058618	1.415803676520733
6	1.0960170698634768	1.068066321339647
7	0.8055309112750545	0.915819535041036
8	0.5454009834762891	0.7086561430399458
9	0.4552482297322392	0.665457658547781
>10	3.0476215131797395	10.661796985459798
>50	1.1637955749208748	13.984981095547088
>100	1.8776117759500963	48.12125173171912
>500	0.004316348910230107	0.5046651176718567
>1k	0.00647452336534516	2.3418424387328876
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCTT	4676	0.40927574997417954	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCT	2960	0.25907960220777837	Illumina Single End Adapter 2 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	2883	0.2523400314746706	No Hit
TGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	2844	0.24892648266179782	No Hit
GCCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	2576	0.22546927543487735	No Hit
TTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	2283	0.1998238958920128	No Hit
GGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	1895	0.1658634615485608	No Hit
ATCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	1690	0.1479204485578194	No Hit
ACCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	1376	0.12043700426956183	No Hit
AGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	1319	0.11544797138920934	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.42511811754161466	0.0
2	0.0	0.0	0.0	0.8838465618561303	0.0
3	0.0	0.0	0.0	2.813989598304079	0.0
4	0.0	0.0	0.0	3.2314928761862083	0.0
5	0.0	0.0	0.0	3.94781296553366	0.0
6	0.0	0.0	0.0	6.1471887237353675	0.0
7	0.0	0.0	0.0	6.43348918955349	0.0
8	0.0	0.0	0.0	6.9732675364505745	0.0
9	0.0	0.0	0.0	7.331515108016938	0.0
10	0.0	0.0	0.0	8.533609451503974	0.0
11	0.0	0.0	0.0	9.547433448927183	0.0
12	0.0	0.0	0.0	9.98655586929084	0.0
13	0.0	0.0	0.0	10.216664070035518	0.0
14	0.0	0.0	0.0	10.349354839274367	0.0
15	0.0	0.0	0.0	10.540250992117327	0.0
16	0.0	0.0	0.0	11.000029759143496	0.0
17	0.0	0.0	0.0	11.627947686926808	0.0
18	0.0	0.0	0.0	12.361773154801813	0.0
19	8.752689263776296E-5	0.0	0.0	12.775162668729967	0.0
20	8.752689263776296E-5	0.0	0.0	13.250696276430933	0.0
21	8.752689263776296E-5	0.0	0.0	13.966228623744646	0.0
22	8.752689263776296E-5	0.0	0.0	14.546094287469826	0.0
23	8.752689263776296E-5	0.0	0.0	15.16333393435133	0.0
24	0.0017505378527552591	0.0	0.0	15.638955068944933	0.0
25	0.0017505378527552591	0.0	0.0	16.057596196431355	0.0
26	0.0017505378527552591	0.0	0.0	16.44814119138105	0.0
27	0.0017505378527552591	0.0	0.0	16.797373493005725	0.0
28	0.0017505378527552591	0.0	0.0	17.165949237903344	0.0
29	0.0017505378527552591	0.0	0.0	17.53505014415679	0.0
30	0.0017505378527552591	0.0	0.0	17.965332348364036	0.0
31	0.0017505378527552591	0.0	0.0	18.34038508331685	0.0
32	0.0017505378527552591	0.0	0.0	18.75114879046587	0.0
33	0.0017505378527552591	0.0	0.0	19.134429053326635	0.0
34	0.0017505378527552591	0.0	0.0	19.506068239466575	0.0
35	0.001838064745393022	0.0	0.0	19.943527648870116	0.0
36	0.001925591638030785	0.0	0.0	20.339936945626544	0.0
37	0.002013118530668548	0.0	0.0	20.74002237187376	0.0
38	0.002100645423306311	0.0	0.0	21.15463726229884	0.0
39	0.002100645423306311	0.0	0.0	21.545357311033815	0.0
40	0.002100645423306311	0.0	0.0	21.98780575331771	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	30	1.8613937E-6	46.0	42
AACGTAC	25	3.417144E-5	46.0	28
CGTGGCA	25	3.417144E-5	46.0	31
AACCGGT	20	6.3114386E-4	46.0	18
ACTATCG	20	6.3114386E-4	46.0	23
GGTACTC	20	6.3114386E-4	46.0	10
AGCCGAC	30	1.8613937E-6	46.0	29
GGTACGC	25	3.417144E-5	46.0	9
TACCCGC	40	5.6115823E-9	46.0	24
GCCCACG	30	1.8613937E-6	46.0	39
CGAACAA	20	6.3114386E-4	46.0	39
CTCCGTA	30	1.8613937E-6	46.0	10
ATCTACG	30	1.8613937E-6	46.0	46
TACCACG	40	5.6115823E-9	46.0	45
ACCGCGA	20	6.3114386E-4	46.0	12
GACCGTA	20	6.3114386E-4	46.0	45
GGCGTAA	30	1.8613937E-6	46.0	31
ACCGAAC	20	6.3114386E-4	46.0	12
ATTCGCG	30	1.8613937E-6	46.0	1
AAGCGTG	25	3.417144E-5	46.0	32
>>END_MODULE