SRR1545610_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1545610_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2243194
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	9825	0.43799154241675037	Illumina PCR Primer Index 2 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6070	0.27059630152363107	No Hit
ATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	4348	0.193830760959596	Illumina PCR Primer Index 2 (95% over 23bp)
ATGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	4288	0.19115600344865402	Illumina PCR Primer Index 2 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3842	0.17127363928398526	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	3681	0.16409637329629093	Illumina PCR Primer Index 2 (96% over 25bp)
ATGCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	3491	0.15562630784497464	Illumina PCR Primer Index 2 (95% over 21bp)
ATTGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	3139	0.13993439711411496	Illumina PCR Primer Index 2 (95% over 21bp)
ATGTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	2873	0.12807630548227214	Illumina PCR Primer Index 2 (95% over 21bp)
ATATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	2787	0.1242424863832553	Illumina PCR Primer Index 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2730	0.12170146674786042	Illumina PCR Primer Index 2 (95% over 24bp)
ATGGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	2570	0.11456878005201511	Illumina PCR Primer Index 2 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGTAC	55	1.8189894E-12	45.000004	42
CAATTCG	20	7.034725E-4	45.0	3
CGCGACA	25	3.8919978E-5	45.0	22
CGCCTAT	25	3.8919978E-5	45.0	21
CCGTATG	25	3.8919978E-5	45.0	13
GCCGATT	25	3.8919978E-5	45.0	31
ACGATAG	35	1.2126293E-7	45.0	15
GTTCGAC	20	7.034725E-4	45.0	45
ACTACCG	25	3.8919978E-5	45.0	3
AATTCGC	40	6.8193913E-9	45.0	15
ACGTATA	40	6.8193913E-9	45.0	12
GTAAGGT	20	7.034725E-4	45.0	44
CGATCAT	25	3.8919978E-5	45.0	28
TACAACG	20	7.034725E-4	45.0	3
ACTATCG	30	2.166429E-6	44.999996	14
CCGATTT	30	2.166429E-6	44.999996	38
ACAATCG	60	0.0	44.999996	25
CATACGT	30	2.166429E-6	44.999996	17
ACTCGAT	65	0.0	44.999996	16
ATACGTA	130	0.0	44.999996	1