##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545610_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2243194
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.879719721076288	31.0	31.0	34.0	31.0	34.0
2	32.54348130389079	34.0	31.0	34.0	31.0	34.0
3	32.50745633235467	34.0	31.0	34.0	31.0	34.0
4	35.99271262316144	37.0	35.0	37.0	35.0	37.0
5	35.756647886896985	37.0	35.0	37.0	35.0	37.0
6	35.754624878632875	37.0	35.0	37.0	35.0	37.0
7	35.74400207917817	37.0	35.0	37.0	33.0	37.0
8	35.71279434591926	37.0	35.0	37.0	33.0	37.0
9	37.88252063798316	39.0	38.0	39.0	35.0	39.0
10	37.82857523691665	39.0	38.0	39.0	35.0	39.0
11	37.63404814741837	39.0	37.0	39.0	35.0	39.0
12	37.160526463605024	39.0	37.0	39.0	33.0	39.0
13	36.91217077078487	39.0	37.0	39.0	33.0	39.0
14	37.75050530627311	40.0	36.0	41.0	33.0	41.0
15	37.68532770683231	40.0	36.0	41.0	33.0	41.0
16	37.72332932416902	40.0	36.0	41.0	33.0	41.0
17	37.7872118951816	40.0	36.0	41.0	33.0	41.0
18	37.82591831112244	40.0	36.0	41.0	33.0	41.0
19	37.79072652655098	40.0	36.0	41.0	33.0	41.0
20	37.73750910532036	40.0	36.0	41.0	33.0	41.0
21	37.69994481083669	40.0	36.0	41.0	33.0	41.0
22	37.61514875663897	40.0	35.0	41.0	32.0	41.0
23	37.56137543163899	40.0	35.0	41.0	32.0	41.0
24	37.498260070239134	40.0	35.0	41.0	32.0	41.0
25	37.429818820842065	39.0	35.0	41.0	32.0	41.0
26	37.36042714094278	39.0	35.0	41.0	32.0	41.0
27	37.253281258776546	39.0	35.0	41.0	32.0	41.0
28	37.17257490881306	39.0	35.0	41.0	31.0	41.0
29	37.13295060525304	39.0	35.0	41.0	31.0	41.0
30	37.028469673153545	39.0	35.0	41.0	31.0	41.0
31	36.96428842088557	39.0	35.0	41.0	31.0	41.0
32	36.85024121854819	39.0	35.0	41.0	31.0	41.0
33	36.74387993191851	39.0	35.0	41.0	31.0	41.0
34	36.67016539808862	39.0	35.0	41.0	30.0	41.0
35	36.57474476126452	39.0	35.0	41.0	30.0	41.0
36	36.47833401836845	39.0	35.0	41.0	30.0	41.0
37	36.351853205741456	38.0	35.0	41.0	30.0	41.0
38	36.280721150288386	38.0	35.0	41.0	30.0	41.0
39	36.2042462667072	38.0	35.0	41.0	30.0	41.0
40	36.11341283901437	38.0	35.0	40.0	30.0	41.0
41	36.020860879620756	38.0	35.0	40.0	30.0	41.0
42	35.89080302461579	38.0	35.0	40.0	29.0	41.0
43	35.76937928685615	38.0	35.0	40.0	29.0	41.0
44	35.72167454085558	38.0	35.0	40.0	29.0	41.0
45	35.69800115371207	38.0	35.0	40.0	29.0	41.0
46	35.60865489119532	37.0	34.0	40.0	29.0	41.0
47	35.51307376892056	37.0	34.0	40.0	29.0	41.0
48	35.45128553303905	37.0	34.0	40.0	28.0	41.0
49	35.395778519379064	37.0	34.0	40.0	28.0	41.0
50	35.3080616299794	37.0	34.0	40.0	28.0	41.0
51	35.122671957931416	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	28.0
11	22.0
12	25.0
13	42.0
14	38.0
15	65.0
16	108.0
17	301.0
18	588.0
19	1135.0
20	2025.0
21	3191.0
22	5233.0
23	7676.0
24	11105.0
25	15882.0
26	20788.0
27	25796.0
28	30967.0
29	37093.0
30	45028.0
31	54994.0
32	68631.0
33	88304.0
34	136540.0
35	244535.0
36	170072.0
37	237197.0
38	353340.0
39	682091.0
40	344.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.1512628867587913	91.07094615980607	3.209441537379291	3.5683494160558564
2	3.1491257555075487	2.97201222899134	90.8567872417633	3.0220747737378044
3	27.40837395249809	16.509851577705717	42.8972260089854	13.184548460810792
4	27.14767425376494	15.909234778623697	42.982417035708906	13.960673931902456
5	24.634026303565363	14.417210459728405	46.583353914106404	14.36540932259983
6	22.013254315052556	15.084785355167677	47.06926819526087	15.832692134518906
7	22.487087608115928	17.887307116548993	44.55352501834438	15.072080256990702
8	25.95495530034406	19.828869014449932	42.383895463343784	11.83228022186222
9	81.41993068811703	4.61244101045206	9.344443681643229	4.623184619787677
10	82.78713298983503	4.340373592297412	9.045628688379159	3.8268647294883986
11	75.28755872207219	7.085923018695664	10.628238128311684	6.998280130920465
12	41.101661291890046	24.355539467384453	16.49366929476452	18.04912994596098
13	30.800144793539925	24.46943955805873	25.889512899909683	18.840902748491658
14	28.105772394184363	22.883620409113078	27.895491874532475	21.115115322170084
15	21.967649699490995	25.912649552379328	31.583358372035587	20.536342376094087
16	17.98743220604192	30.34405405863247	30.21521990518876	21.45329383013685
17	17.127319349106678	24.444742630374368	37.10820374876181	21.319734271757145
18	20.836048955195135	20.99131862870532	36.12487372915584	22.047758686943705
19	20.99225479383415	22.290760406812783	28.4614705638478	28.255514235505263
20	22.51004594341818	22.433191244270446	31.607520348217765	23.449242464093608
21	25.5565947483811	24.547587056670086	27.45215081709384	22.443667377854972
22	26.661537076151237	21.895208350236313	27.327284220624698	24.11597035298775
23	25.37408712755116	24.500689641644904	27.91176331605737	22.21345991474656
24	28.278294253640123	21.239446967137038	25.097338883752364	25.384919895470475
25	25.540769099774696	25.170983873886964	24.419956544106306	24.86829048223203
26	25.48080995223775	20.54222684261816	30.292119183628348	23.68484402151575
27	26.329688827627034	23.295755962257388	26.209369318926495	24.165185891189083
28	22.779438604061887	26.023117037581233	25.569567322309172	25.627877036047707
29	22.077805129649956	24.97755432655401	25.959324070945268	26.98531647285077
30	22.507192868739843	24.132197215220796	29.421530193108573	23.939079722930785
31	21.551992382290607	23.574287377730148	28.780123341984687	26.09359689799456
32	22.92819970096211	26.055347865588086	26.700856011562085	24.31559642188772
33	23.666165298231004	25.894327463429377	25.522536169408443	24.916971068931176
34	25.00113677194215	26.136259280294084	25.9417152506649	22.920888697098867
35	26.079420683186566	26.287383079662302	24.1482011809946	23.484995056156535
36	22.12416759317295	24.483704931450422	26.560252925070234	26.831874550306395
37	22.900783436474956	25.189083066377677	27.75029712098017	24.1598363761672
38	24.59452905098712	28.050003700081223	23.60000962912704	23.755457619804616
39	24.098227794831832	26.79745933699894	26.191493022895035	22.912819845274193
40	23.31875887685149	25.667864660836287	27.474083828683565	23.539292633628655
41	22.312336784067718	26.773743153735253	25.275433154689253	25.63848690750778
42	25.06252245681827	24.382688256120513	26.783283122190948	23.77150616487027
43	21.264545108448043	24.29540200268011	28.54010843466949	25.899944454202355
44	21.173157560157527	25.01130085048373	25.838202134991445	27.9773394543673
45	22.09122349649651	22.869845407931727	27.25622482941734	27.78270626615442
46	23.752604545126278	23.666566511857646	27.108801111272584	25.47202783174349
47	21.464795287433898	25.19068792088424	27.144999496254	26.19951729542786
48	21.425298034855654	25.473811003417445	25.921476252165437	27.179414709561456
49	22.2532246430759	24.6898841562522	29.02481907494403	24.03207212572787
50	21.940857545089727	23.737001792979118	29.657087171238867	24.66505349069229
51	20.797755343496817	23.955083688704587	27.45023390754433	27.796927060254262
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1033.0
1	1735.5
2	2438.0
3	6226.5
4	10015.0
5	6923.5
6	3832.0
7	3995.5
8	4159.0
9	4312.5
10	4466.0
11	4499.5
12	4533.0
13	4748.5
14	4964.0
15	5047.0
16	5130.0
17	5350.0
18	5570.0
19	6256.0
20	6942.0
21	7305.5
22	7669.0
23	8619.0
24	9569.0
25	12918.0
26	20872.0
27	25477.0
28	28252.0
29	31027.0
30	35389.5
31	39752.0
32	46137.5
33	52523.0
34	60153.0
35	67783.0
36	70654.0
37	73525.0
38	85026.0
39	96527.0
40	108527.5
41	120528.0
42	137506.5
43	154485.0
44	173320.0
45	192155.0
46	201163.0
47	210171.0
48	201693.0
49	193215.0
50	167477.5
51	141740.0
52	133382.5
53	125025.0
54	123712.5
55	122400.0
56	123659.5
57	124919.0
58	117758.0
59	110597.0
60	99310.5
61	88024.0
62	74075.0
63	60126.0
64	51545.0
65	42964.0
66	37145.0
67	31326.0
68	26305.0
69	21284.0
70	16756.0
71	12228.0
72	10957.5
73	9687.0
74	6634.0
75	3192.0
76	2803.0
77	2087.5
78	1372.0
79	1072.0
80	772.0
81	501.0
82	230.0
83	210.0
84	190.0
85	123.0
86	56.0
87	48.0
88	40.0
89	55.0
90	70.0
91	37.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2243194.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.725664321623082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.5457322541706	11.251041690738083
2	11.096693186634251	3.490057442661043
3	4.772397608717608	2.2514736841202936
4	2.684472664288994	1.6886046399672752
5	1.6588377062831394	1.3043162466529918
6	1.1014197431237165	1.039233429454313
7	0.7851396569376544	0.8642789883417099
8	0.5549189398248	0.698117517871661
9	0.39630119375288503	0.5608889588894742
>10	2.6854557818828733	9.004776895448604
>50	0.8832671120985056	10.215302776528844
>100	1.816305039112271	53.41166565818496
>500	0.012516432531298473	1.256217740343795
>1k	0.005973751889937908	2.2529787862215973
>5k	5.689287514226579E-4	0.7110455445753373
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	9825	0.43799154241675037	Illumina PCR Primer Index 2 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6070	0.27059630152363107	No Hit
ATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	4348	0.193830760959596	Illumina PCR Primer Index 2 (95% over 23bp)
ATGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	4288	0.19115600344865402	Illumina PCR Primer Index 2 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3842	0.17127363928398526	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	3681	0.16409637329629093	Illumina PCR Primer Index 2 (96% over 25bp)
ATGCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	3491	0.15562630784497464	Illumina PCR Primer Index 2 (95% over 21bp)
ATTGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	3139	0.13993439711411496	Illumina PCR Primer Index 2 (95% over 21bp)
ATGTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	2873	0.12807630548227214	Illumina PCR Primer Index 2 (95% over 21bp)
ATATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	2787	0.1242424863832553	Illumina PCR Primer Index 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2730	0.12170146674786042	Illumina PCR Primer Index 2 (95% over 24bp)
ATGGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	2570	0.11456878005201511	Illumina PCR Primer Index 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17987744261084865	0.0
2	0.0	0.0	0.0	0.8161576751720984	0.0
3	0.0	0.0	0.0	1.099815709207496	0.0
4	0.0	0.0	0.0	1.4704925209322064	0.0
5	0.0	0.0	0.0	2.8598953099910216	0.0
6	0.0	0.0	0.0	3.1758733306169686	0.0
7	0.0	0.0	0.0	3.7085512889210652	0.0
8	4.457929184903312E-5	0.0	0.0	5.220324234105476	0.0
9	4.457929184903312E-5	0.0	0.0	5.408315107832849	0.0
10	8.915858369806624E-5	0.0	0.0	5.799632131683662	0.0
11	8.915858369806624E-5	0.0	0.0	6.08373595863755	0.0
12	8.915858369806624E-5	0.0	0.0	6.871407466318116	0.0
13	1.7831716739613249E-4	0.0	0.0	7.562475648561827	0.0
14	1.7831716739613249E-4	0.0	0.0	7.852909734958279	0.0
15	1.7831716739613249E-4	0.0	0.0	8.024049636366716	0.0
16	2.674757510941987E-4	0.0	0.0	8.126894062662435	0.0
17	3.1205504294323186E-4	0.0	0.0	8.287869885529295	0.0
18	3.5663433479226497E-4	0.0	0.0	8.622214574397043	0.0
19	4.012136266412981E-4	0.0	0.0	9.050532410482553	0.0
20	4.457929184903312E-4	0.0	0.0	9.502833905582843	0.0
21	4.903722103393643E-4	0.0	0.0	9.824428916981768	4.457929184903312E-5
22	4.903722103393643E-4	0.0	0.0	10.162429107781136	4.457929184903312E-5
23	4.903722103393643E-4	0.0	0.0	10.651642256532426	4.457929184903312E-5
24	4.903722103393643E-4	0.0	0.0	11.07608169422707	4.457929184903312E-5
25	4.903722103393643E-4	0.0	0.0	11.547552284822444	4.457929184903312E-5
26	4.903722103393643E-4	0.0	0.0	11.907663804378934	4.457929184903312E-5
27	6.241100858864637E-4	0.0	0.0	12.245173622967965	4.457929184903312E-5
28	7.132686695845299E-4	0.0	0.0	12.52753885753974	4.457929184903312E-5
29	7.132686695845299E-4	0.0	0.0	12.809592037068573	4.457929184903312E-5
30	7.57847961433563E-4	0.0	0.0	13.105643114238001	4.457929184903312E-5
31	8.024272532825962E-4	0.0	0.0	13.408247347309239	4.457929184903312E-5
32	8.024272532825962E-4	0.0	0.0	13.74428604926725	4.457929184903312E-5
33	8.024272532825962E-4	0.0	0.0	14.063652096073723	4.457929184903312E-5
34	8.024272532825962E-4	0.0	0.0	14.38662906551997	4.457929184903312E-5
35	8.470065451316293E-4	0.0	0.0	14.710497620803194	4.457929184903312E-5
36	8.470065451316293E-4	0.0	0.0	15.018986320398502	4.457929184903312E-5
37	8.915858369806624E-4	0.0	0.0	15.405889994356261	4.457929184903312E-5
38	9.361651288296955E-4	0.0	0.0	15.741393744812084	4.457929184903312E-5
39	9.361651288296955E-4	0.0	0.0	16.09200987520473	4.457929184903312E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGTAC	55	1.8189894E-12	45.000004	42
CAATTCG	20	7.034725E-4	45.0	3
CGCGACA	25	3.8919978E-5	45.0	22
CGCCTAT	25	3.8919978E-5	45.0	21
CCGTATG	25	3.8919978E-5	45.0	13
GCCGATT	25	3.8919978E-5	45.0	31
ACGATAG	35	1.2126293E-7	45.0	15
GTTCGAC	20	7.034725E-4	45.0	45
ACTACCG	25	3.8919978E-5	45.0	3
AATTCGC	40	6.8193913E-9	45.0	15
ACGTATA	40	6.8193913E-9	45.0	12
GTAAGGT	20	7.034725E-4	45.0	44
CGATCAT	25	3.8919978E-5	45.0	28
TACAACG	20	7.034725E-4	45.0	3
ACTATCG	30	2.166429E-6	44.999996	14
CCGATTT	30	2.166429E-6	44.999996	38
ACAATCG	60	0.0	44.999996	25
CATACGT	30	2.166429E-6	44.999996	17
ACTCGAT	65	0.0	44.999996	16
ATACGTA	130	0.0	44.999996	1
>>END_MODULE