FastQCFastQC Report
Thu 26 May 2016
SRR1545609_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545609_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1876428
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTT79180.4219719594889865Illumina PCR Primer Index 2 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT67750.3610583512929886Illumina PCR Primer Index 2 (96% over 25bp)
GTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC62030.3305749008222005Illumina PCR Primer Index 2 (95% over 24bp)
TGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC61800.32934916767389955Illumina PCR Primer Index 2 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC54900.2925771732248719Illumina PCR Primer Index 2 (95% over 24bp)
TTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC52710.28090606194322404Illumina PCR Primer Index 2 (95% over 24bp)
GGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC47180.2514351736384236Illumina PCR Primer Index 2 (95% over 24bp)
ATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC44280.2359802774207164Illumina PCR Primer Index 2 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC29670.15811957613081876Illumina PCR Primer Index 2 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC28870.1538561564845547Illumina PCR Primer Index 2 (95% over 24bp)
TCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC20860.11116866727633568Illumina PCR Primer Index 2 (95% over 24bp)
CTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC19720.10509329428040938Illumina PCR Primer Index 2 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA206.312482E-446.038
GGTACGA206.312482E-446.08
GTCGATC206.312482E-446.037
GTCGACA206.312482E-446.038
ACGTTAT206.312482E-446.042
GACGTAT206.312482E-446.09
CGGTTAC206.312482E-446.045
ATTACCG206.312482E-446.016
CAATTCG253.4179888E-546.01
TAACGTG301.8620376E-646.017
CGCGAAA301.8620376E-646.020
GTTACGT206.312482E-446.037
GTTACGC301.8620376E-646.040
CCGTATG206.312482E-446.011
ACGATAG301.8620376E-646.013
ACAATCG501.6370905E-1146.023
AAACCGG206.312482E-446.030
ACTACGT206.312482E-446.014
CGTACGA301.8620376E-646.034
CGTAATA301.8620376E-646.015
Produced by FastQC (version 0.11.5)