##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545609_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1876428
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51423076185177	33.0	31.0	34.0	31.0	34.0
2	32.930668269712456	34.0	31.0	34.0	31.0	34.0
3	33.01499711153319	34.0	33.0	34.0	31.0	34.0
4	36.3997179747904	37.0	37.0	37.0	35.0	37.0
5	36.37854956331924	37.0	37.0	37.0	35.0	37.0
6	36.38566787534614	37.0	37.0	37.0	35.0	37.0
7	36.46669469865084	37.0	37.0	37.0	35.0	37.0
8	36.50560479805247	37.0	37.0	37.0	35.0	37.0
9	38.304460389633924	39.0	39.0	39.0	37.0	39.0
10	38.054854223023746	39.0	39.0	39.0	35.0	39.0
11	37.89256289076906	39.0	38.0	39.0	35.0	39.0
12	37.614242059913835	39.0	37.0	39.0	35.0	39.0
13	37.573357997215986	39.0	37.0	39.0	35.0	39.0
14	38.95667566248212	41.0	38.0	41.0	35.0	41.0
15	38.96780585239615	41.0	38.0	41.0	35.0	41.0
16	39.01731054961874	41.0	38.0	41.0	35.0	41.0
17	38.96667284862515	41.0	38.0	41.0	35.0	41.0
18	38.95665168074661	41.0	38.0	41.0	35.0	41.0
19	38.91474173269638	41.0	38.0	41.0	35.0	41.0
20	38.834790356997445	40.0	38.0	41.0	35.0	41.0
21	38.79339255223222	40.0	38.0	41.0	35.0	41.0
22	38.86240186140902	41.0	38.0	41.0	35.0	41.0
23	38.845957851833376	41.0	38.0	41.0	35.0	41.0
24	38.82735815069909	41.0	38.0	41.0	35.0	41.0
25	38.73533596812668	40.0	38.0	41.0	35.0	41.0
26	38.62984404410934	40.0	38.0	41.0	35.0	41.0
27	38.584546809150154	40.0	38.0	41.0	35.0	41.0
28	38.52574625831633	40.0	37.0	41.0	35.0	41.0
29	38.49184247943433	40.0	37.0	41.0	35.0	41.0
30	38.41147701910225	40.0	37.0	41.0	35.0	41.0
31	38.328335006725545	40.0	37.0	41.0	35.0	41.0
32	38.27390232931933	40.0	37.0	41.0	34.0	41.0
33	38.21248990102471	40.0	36.0	41.0	34.0	41.0
34	38.10671712423818	40.0	36.0	41.0	34.0	41.0
35	38.05804166213678	40.0	36.0	41.0	34.0	41.0
36	37.988553784104695	40.0	36.0	41.0	34.0	41.0
37	37.87908195784757	40.0	35.0	41.0	34.0	41.0
38	37.774810970631435	40.0	35.0	41.0	34.0	41.0
39	37.66217675285169	40.0	35.0	41.0	33.0	41.0
40	37.54426761911461	40.0	35.0	41.0	33.0	41.0
41	37.40938741054813	39.0	35.0	41.0	33.0	41.0
42	37.37586307601464	39.0	35.0	41.0	33.0	41.0
43	37.3123141415498	39.0	35.0	41.0	33.0	41.0
44	37.17161543102107	39.0	35.0	41.0	33.0	41.0
45	37.07925537244168	39.0	35.0	41.0	33.0	41.0
46	37.04954360092687	39.0	35.0	41.0	33.0	41.0
47	37.00246265777317	39.0	35.0	41.0	33.0	41.0
48	36.92448631122537	39.0	35.0	41.0	33.0	41.0
49	36.849719253816296	38.0	35.0	41.0	33.0	41.0
50	36.74070574517114	38.0	35.0	41.0	33.0	41.0
51	36.63785767426195	38.0	35.0	40.0	32.0	41.0
52	36.32347897174845	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	7.0
11	4.0
12	6.0
13	13.0
14	23.0
15	40.0
16	71.0
17	138.0
18	239.0
19	458.0
20	758.0
21	1263.0
22	1956.0
23	2863.0
24	3915.0
25	5254.0
26	6657.0
27	8367.0
28	11010.0
29	14129.0
30	17939.0
31	23445.0
32	30456.0
33	42754.0
34	90554.0
35	183320.0
36	114254.0
37	170918.0
38	288336.0
39	856041.0
40	1239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.635059805119088	13.915375383441306	46.2261275146182	12.223437296821407
2	25.469562381290412	13.354149479756217	48.67125197449622	12.50503616445715
3	25.769174196931615	13.841138588850733	45.4765650480594	14.913122166158255
4	22.7110232846664	14.492429232563147	47.18651608268476	15.610031400085694
5	23.131236583551303	17.32024889843895	44.10566246080319	15.442852057206565
6	25.324819284299743	19.148936170212767	43.33611521465252	12.19012933083497
7	85.26274389425014	3.1266320903333353	6.729221691426476	4.8814023239900495
8	86.80215814302494	2.829844790207778	6.891764565440293	3.4762325013269897
9	78.46594700143038	4.678356963336723	9.305073256208072	7.5506227790248275
10	42.04072844788076	23.581240527214472	14.688813000019184	19.68921802488558
11	30.791962174940895	23.10453691801657	26.692364428584526	19.41113647845801
12	28.209608895198752	22.853261622614884	27.075912318511552	21.861217163674812
13	22.38130106777345	24.458758875906774	32.894680744478336	20.265259311841437
14	17.84182499941378	31.031140017096316	29.605505780131185	21.52152920335872
15	17.178703366182983	24.093383812221944	35.92533260002516	22.80258022156992
16	20.780973210802653	22.01091648600426	34.50524080860017	22.702869494592917
17	20.917722395956574	22.949348442892557	26.73702374937914	29.39590541177173
18	22.34207760702782	22.962405165559243	29.667272072256434	25.028245155156498
19	24.736680544097613	25.307978776697	27.032372145374083	22.922968533831302
20	26.827834587844563	21.141605220131016	26.09122225846129	25.939337933563134
21	25.29833278974733	25.091397058666786	27.576757541456427	22.033512610129456
22	28.312623772401608	21.001498592005664	24.381537687563817	26.304339948028915
23	25.730110614422724	25.702664850449896	22.28276278119917	26.284461753928205
24	25.97296565602304	19.88251081309808	29.23245656108308	24.912066969795806
25	28.45923211548751	22.98729287774431	23.633893759845833	24.91958124692234
26	22.59713668736557	26.001210811179536	23.872805138273357	27.528847363181537
27	21.795880257595815	23.74458279241197	23.791640286757605	30.66789666323461
28	23.467353929913646	22.789150449684186	27.530712609276776	26.212783011125396
29	22.13977834481259	21.294928449159787	28.443084413577285	28.122208792450337
30	24.181316842426142	24.50480380808643	25.34693577371474	25.966943575772696
31	22.88102714306118	25.754731862879897	24.944682130089724	26.4195588639692
32	25.048389812985093	26.404103967751492	24.6694250991778	23.87808112008561
33	26.546928525901343	26.59323992180888	23.17664200278401	23.683189549505762
34	20.83970181642994	23.92012909634689	26.488679554984255	28.75148953223891
35	22.39355839925646	24.88179669030733	26.746030223381872	25.978614687054343
36	23.796703097587542	26.76798683456013	23.272249188351484	26.163060879500836
37	24.921393200272007	25.726966342433606	24.832554193392976	24.519086263901414
38	22.81030766967877	24.399177586350234	29.212738245219107	23.577776498751884
39	22.35188347221423	27.0883295282313	24.74558043260919	25.81420656694528
40	25.62134012069741	24.09141198063555	26.88848173231267	23.39876616635437
41	21.19916138535558	23.71345982899424	27.684462180270174	27.402916605380007
42	20.900455546389203	24.660205454192756	24.638408721251228	29.800930278166817
43	22.18896754898136	21.31736469504825	27.458181182544706	29.03548657342568
44	26.557960124236047	21.526751892425395	25.21620866881117	26.699079314527392
45	21.756763382341344	23.22130132357863	26.650423037814402	28.37151225626563
46	21.456139004534148	24.20993504680169	25.30328901508611	29.03063693357805
47	22.806204128269243	23.523950825717797	28.959970752941228	24.709874293071728
48	21.333885446177526	22.033406024638303	31.324196825031393	25.308511704152785
49	21.072964163826164	21.93156358783817	26.72966935048933	30.265802897846335
50	20.07505750287248	22.469500561705537	31.594710801586846	25.860731133835134
51	22.195202800214023	23.43841596906463	27.41810503786983	26.948276192851523
52	21.012849946813837	21.984749747925314	27.962223970224276	29.04017633503657
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	274.0
1	221.0
2	168.0
3	140.5
4	113.0
5	173.0
6	233.0
7	390.0
8	547.0
9	827.0
10	1107.0
11	1127.0
12	1147.0
13	1239.0
14	1444.0
15	1557.0
16	1737.0
17	1917.0
18	2029.0
19	2141.0
20	2349.0
21	2557.0
22	3090.5
23	3624.0
24	4232.5
25	4841.0
26	8269.5
27	11698.0
28	12999.5
29	14301.0
30	16710.5
31	19120.0
32	25612.5
33	32105.0
34	34993.0
35	37881.0
36	40705.0
37	43529.0
38	47499.5
39	58865.5
40	66261.0
41	77297.0
42	88333.0
43	101063.5
44	113794.0
45	128889.5
46	143985.0
47	165015.0
48	186045.0
49	188882.0
50	191719.0
51	173733.5
52	155748.0
53	133687.0
54	111626.0
55	108762.0
56	105898.0
57	100868.5
58	95839.0
59	96746.5
60	97654.0
61	87388.5
62	77123.0
63	71244.0
64	54172.5
65	42980.0
66	39455.0
67	35930.0
68	29706.0
69	23482.0
70	19948.5
71	16415.0
72	13644.0
73	10873.0
74	8765.0
75	6657.0
76	5475.5
77	4294.0
78	3314.5
79	2335.0
80	1742.5
81	1150.0
82	857.0
83	564.0
84	488.5
85	413.0
86	232.0
87	51.0
88	48.0
89	44.0
90	43.0
91	29.5
92	16.0
93	66.5
94	117.0
95	63.0
96	9.0
97	6.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1876428.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.201365745767788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.42173057403974	10.92324116242632
2	12.407757094288534	4.02045221538427
3	5.639263865227262	2.7409132925231705
4	3.120774662848788	2.022432468917535
5	1.9245487606483302	1.5590159183413852
6	1.229986906707956	1.1956480642848697
7	0.9324502663328014	1.0574877463217411
8	0.6440240134034125	0.8347254872164753
9	0.49189587422209274	0.7172446470395683
>10	3.330940060688925	11.388043109767748
>50	1.107928198119288	13.342525868596569
>100	1.7342040965264	45.8759817726202
>500	0.008565597741163791	0.8672865117109867
>1k	0.0039533528036140575	1.4354608558037587
>5k	0.0019766764018070287	2.019540879045394
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTT	7918	0.4219719594889865	Illumina PCR Primer Index 2 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	6775	0.3610583512929886	Illumina PCR Primer Index 2 (96% over 25bp)
GTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	6203	0.3305749008222005	Illumina PCR Primer Index 2 (95% over 24bp)
TGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	6180	0.32934916767389955	Illumina PCR Primer Index 2 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	5490	0.2925771732248719	Illumina PCR Primer Index 2 (95% over 24bp)
TTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	5271	0.28090606194322404	Illumina PCR Primer Index 2 (95% over 24bp)
GGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	4718	0.2514351736384236	Illumina PCR Primer Index 2 (95% over 24bp)
ATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	4428	0.2359802774207164	Illumina PCR Primer Index 2 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2967	0.15811957613081876	Illumina PCR Primer Index 2 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2887	0.1538561564845547	Illumina PCR Primer Index 2 (95% over 24bp)
TCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2086	0.11116866727633568	Illumina PCR Primer Index 2 (95% over 24bp)
CTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1972	0.10509329428040938	Illumina PCR Primer Index 2 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4429160085012588	0.0
2	0.0	0.0	0.0	1.0338792642190375	0.0
3	5.32927455783009E-5	0.0	0.0	3.6426124530224446	0.0
4	5.32927455783009E-5	0.0	0.0	4.2112460483429155	0.0
5	5.32927455783009E-5	0.0	0.0	5.135608720398545	0.0
6	5.32927455783009E-5	0.0	0.0	7.879492311988523	0.0
7	5.32927455783009E-5	0.0	0.0	8.220459298198492	0.0
8	5.32927455783009E-5	0.0	0.0	8.89237423444971	0.0
9	5.32927455783009E-5	0.0	0.0	9.337048903555052	0.0
10	5.32927455783009E-5	0.0	0.0	10.734864327328307	0.0
11	5.32927455783009E-5	0.0	0.0	11.927875729844152	0.0
12	5.32927455783009E-5	0.0	0.0	12.434369983820323	0.0
13	5.32927455783009E-5	0.0	0.0	12.689961991613854	0.0
14	5.32927455783009E-5	0.0	0.0	12.826924347750086	0.0
15	5.32927455783009E-5	0.0	0.0	13.057948399832021	0.0
16	5.32927455783009E-5	0.0	0.0	13.589756707957886	0.0
17	5.32927455783009E-5	0.0	0.0	14.298283760421397	0.0
18	5.32927455783009E-5	0.0	0.0	15.060316729445521	5.32927455783009E-5
19	4.263419646264072E-4	0.0	0.0	15.566757690676114	5.32927455783009E-5
20	4.263419646264072E-4	0.0	0.0	16.11577955562377	5.32927455783009E-5
21	4.263419646264072E-4	0.0	0.0	16.91501086106155	5.32927455783009E-5
22	4.263419646264072E-4	0.0	0.0	17.58053066784337	5.32927455783009E-5
23	4.263419646264072E-4	0.0	0.0	18.300622246097372	5.32927455783009E-5
24	4.263419646264072E-4	0.0	0.0	18.8492177690804	5.32927455783009E-5
25	4.263419646264072E-4	0.0	0.0	19.3517150671382	5.32927455783009E-5
26	4.263419646264072E-4	0.0	0.0	19.760203961995877	5.32927455783009E-5
27	4.263419646264072E-4	0.0	0.0	20.169279077054917	5.32927455783009E-5
28	4.263419646264072E-4	0.0	0.0	20.59844555719697	5.32927455783009E-5
29	4.263419646264072E-4	0.0	0.0	21.031555700511824	5.32927455783009E-5
30	4.263419646264072E-4	0.0	0.0	21.518065174896133	5.32927455783009E-5
31	4.263419646264072E-4	0.0	0.0	21.954799224910307	5.32927455783009E-5
32	4.263419646264072E-4	0.0	0.0	22.40080621265511	5.32927455783009E-5
33	4.263419646264072E-4	0.0	0.0	22.83237086634819	5.32927455783009E-5
34	4.263419646264072E-4	0.0	0.0	23.235157437428988	5.32927455783009E-5
35	4.796347102047081E-4	0.0	0.0	23.740905592967064	5.32927455783009E-5
36	4.796347102047081E-4	0.0	0.0	24.18733892267649	5.32927455783009E-5
37	4.796347102047081E-4	0.0	0.0	24.647255317017226	5.32927455783009E-5
38	4.796347102047081E-4	0.0	0.0	25.08878571413345	5.32927455783009E-5
39	4.796347102047081E-4	0.0	0.0	25.516406704653736	5.32927455783009E-5
40	4.796347102047081E-4	0.0	0.0	25.966836990281536	5.32927455783009E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGTA	20	6.312482E-4	46.0	38
GGTACGA	20	6.312482E-4	46.0	8
GTCGATC	20	6.312482E-4	46.0	37
GTCGACA	20	6.312482E-4	46.0	38
ACGTTAT	20	6.312482E-4	46.0	42
GACGTAT	20	6.312482E-4	46.0	9
CGGTTAC	20	6.312482E-4	46.0	45
ATTACCG	20	6.312482E-4	46.0	16
CAATTCG	25	3.4179888E-5	46.0	1
TAACGTG	30	1.8620376E-6	46.0	17
CGCGAAA	30	1.8620376E-6	46.0	20
GTTACGT	20	6.312482E-4	46.0	37
GTTACGC	30	1.8620376E-6	46.0	40
CCGTATG	20	6.312482E-4	46.0	11
ACGATAG	30	1.8620376E-6	46.0	13
ACAATCG	50	1.6370905E-11	46.0	23
AAACCGG	20	6.312482E-4	46.0	30
ACTACGT	20	6.312482E-4	46.0	14
CGTACGA	30	1.8620376E-6	46.0	34
CGTAATA	30	1.8620376E-6	46.0	15
>>END_MODULE