##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545608_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3456687 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.904634408611482 31.0 31.0 34.0 31.0 34.0 2 32.581058105636984 34.0 31.0 34.0 31.0 34.0 3 32.53277806176839 34.0 31.0 34.0 31.0 34.0 4 35.99819017458046 37.0 35.0 37.0 35.0 37.0 5 35.76539848704844 37.0 35.0 37.0 35.0 37.0 6 35.75907480197079 37.0 35.0 37.0 35.0 37.0 7 35.73713732252877 37.0 35.0 37.0 33.0 37.0 8 35.70298699303697 37.0 35.0 37.0 33.0 37.0 9 37.92297133064116 39.0 38.0 39.0 35.0 39.0 10 37.90742957056858 39.0 38.0 39.0 35.0 39.0 11 37.710429668639364 39.0 38.0 39.0 35.0 39.0 12 37.16264388415844 39.0 37.0 39.0 33.0 39.0 13 36.86272983350821 39.0 37.0 39.0 32.0 39.0 14 37.514797261076865 39.0 35.0 41.0 33.0 41.0 15 37.430484160122106 40.0 35.0 41.0 32.0 41.0 16 37.46260827202463 40.0 35.0 41.0 32.0 41.0 17 37.50409626327174 40.0 35.0 41.0 32.0 41.0 18 37.546432176242746 40.0 35.0 41.0 33.0 41.0 19 37.46227934435487 39.0 35.0 41.0 32.0 41.0 20 37.415056382021284 39.0 35.0 41.0 32.0 41.0 21 37.413924373251035 39.0 35.0 41.0 32.0 41.0 22 37.37398005662647 39.0 35.0 41.0 32.0 41.0 23 37.32723327278403 39.0 35.0 41.0 32.0 41.0 24 37.275403008719046 39.0 35.0 41.0 32.0 41.0 25 37.22128095485648 39.0 35.0 41.0 32.0 41.0 26 37.152135556386796 39.0 35.0 41.0 32.0 41.0 27 37.07763069088986 39.0 35.0 41.0 31.0 41.0 28 36.97900967024206 39.0 35.0 41.0 31.0 41.0 29 36.946351810273825 39.0 35.0 41.0 31.0 41.0 30 36.871205000626325 39.0 35.0 41.0 31.0 41.0 31 36.79579117229879 39.0 35.0 41.0 31.0 41.0 32 36.66370024245759 39.0 35.0 41.0 31.0 41.0 33 36.56634054515205 39.0 35.0 41.0 30.0 41.0 34 36.51008610267577 38.0 35.0 41.0 30.0 41.0 35 36.404137834869054 38.0 35.0 41.0 30.0 41.0 36 36.253349522244854 38.0 35.0 41.0 30.0 41.0 37 36.141051532869476 38.0 35.0 41.0 30.0 41.0 38 36.09049300674316 38.0 35.0 40.0 30.0 41.0 39 36.059835038578846 38.0 35.0 40.0 30.0 41.0 40 36.00400614808341 38.0 35.0 40.0 30.0 41.0 41 35.94926471502916 38.0 35.0 40.0 30.0 41.0 42 35.842548081443304 38.0 35.0 40.0 29.0 41.0 43 35.72279815904651 38.0 35.0 40.0 29.0 41.0 44 35.63426801443116 37.0 34.0 40.0 29.0 41.0 45 35.60939246162583 37.0 34.0 40.0 29.0 41.0 46 35.51750534543625 37.0 34.0 40.0 29.0 41.0 47 35.444694876915385 37.0 34.0 40.0 28.0 41.0 48 35.367235158983156 37.0 34.0 40.0 28.0 41.0 49 35.333556957861674 37.0 34.0 40.0 28.0 41.0 50 35.229862871587734 36.0 34.0 40.0 28.0 41.0 51 35.03972676727745 36.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 8.0 10 22.0 11 31.0 12 35.0 13 29.0 14 60.0 15 96.0 16 185.0 17 437.0 18 924.0 19 1765.0 20 3127.0 21 5128.0 22 8077.0 23 12066.0 24 17283.0 25 25015.0 26 32887.0 27 41726.0 28 49960.0 29 60125.0 30 72974.0 31 89380.0 32 111003.0 33 142933.0 34 231763.0 35 414354.0 36 255775.0 37 347775.0 38 515793.0 39 1015433.0 40 512.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 1.8658617340823742 91.73905534403318 3.2106464947506095 3.1844364271338423 2 2.9998666353071597 2.5729549710459754 91.71481247795938 2.7123659156874775 3 25.298414348768056 15.874824651465405 44.77249458802605 14.05426641174049 4 25.65855687830573 15.44423894902836 45.66114895563295 13.236055217032957 5 23.20930416899187 13.820950522856135 49.066635191441975 13.90311011671002 6 21.814182192370904 14.185143173217593 49.354396275971766 14.646278358439742 7 21.519506973006234 16.773604321131767 47.06865273020091 14.638235975661088 8 24.75228448511537 19.226733574662674 44.14073359838481 11.880248341837142 9 84.27693916168863 3.294426136934007 8.61709492354963 3.811539777827729 10 86.01094053352242 3.2212346677613564 7.522462982618906 3.245361816097321 11 79.93506499142097 5.785279372995009 8.648628007106227 5.6310276284777885 12 45.56860369480951 24.81511921675292 13.18916060378044 16.42711648465713 13 34.70745254053954 23.099430176929527 23.950389491440795 18.24272779109014 14 29.598659062854114 22.159917863549694 28.00910235725711 20.232320716339082 15 22.775709805371445 24.698938607979258 32.199964879666574 20.325386706982727 16 19.086744041332064 28.931372727701408 31.344058631863398 20.63782459910313 17 19.38448578074902 23.170017997001175 36.67905135755711 20.766444864692694 18 24.60998059702831 20.856704700194147 33.47795157617684 21.055363126600703 19 23.332167477124774 22.11065103667182 29.021950786981872 25.53523069922154 20 24.83276617177083 22.05282109719509 30.79711874404596 22.31729398698812 21 25.115233169795243 25.77398532178354 26.90301436028197 22.207767148139244 22 26.76756674816088 23.509302404296367 26.91614832352481 22.80698252401794 23 25.78466028309766 26.35835989778652 27.647600144300018 20.2093796748158 24 29.40026100135766 21.070117138173053 26.908887035476454 22.620734824992834 25 27.816258747176125 23.217664775549537 24.64142689228154 24.324649584992798 26 28.21745214420629 20.658480215304422 28.60921454560393 22.51485309488536 27 27.99975814992795 22.278731050858813 24.461312233361017 25.260198565852214 28 23.134695157530896 24.789024866873973 25.87992491075993 26.196355064835203 29 20.858266889654747 23.55446125148155 26.81807754072035 28.769194318143356 30 21.496363425441757 24.61148492762 28.4608933351501 25.431258311788135 31 22.595595146450922 22.491998841665445 28.215861025311227 26.696544986572402 32 23.61674632386444 25.036313672600386 26.81613926861182 24.530800734923353 33 26.05011677366218 23.78314843085301 26.24351004299782 23.92322475248699 34 27.181604814089326 23.462986379732964 25.98198795551926 23.373420850658448 35 27.445238750283146 25.48023584432146 24.63893896091836 22.43558644447704 36 23.053027364062757 23.254896957693884 29.53148491604823 24.16059076219513 37 22.192289900705504 24.713085101428046 28.71107508432207 24.383549913544385 38 25.057981819007622 27.508131340789603 25.12634207262619 22.307544767576584 39 24.14985794201211 27.065771358529133 26.96613838626407 21.818232313194684 40 23.04243919105201 26.733256438896554 27.26364869020539 22.96065567984605 41 23.418261474064618 26.76588884096246 24.700558656308772 25.11529102866415 42 25.832017767301462 23.432321179210035 25.169475859399476 25.56618519408902 43 21.815744381831507 24.032578014729133 27.4584305723949 26.693247031044464 44 21.086982998460666 23.696851927871975 25.849491145712644 29.366673927954718 45 21.05628886850328 21.87308252092249 27.398459854768454 29.672168755805778 46 22.810367267849244 23.316343076477565 26.81663106899757 27.056658586675624 47 21.234812408528743 24.302084626117434 27.02463370273328 27.43846926262054 48 22.170997836946185 24.345015906849536 26.2689968747532 27.214989381451083 49 21.356373892111147 23.814392220065052 29.17666540245038 25.652568485373422 50 21.791646162929997 22.417158394728826 30.825324942640165 24.965870499701015 51 22.051519272644587 22.21803709737098 29.010928672454288 26.719514957530144 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1047.0 1 1893.0 2 2739.0 3 8870.0 4 15001.0 5 10103.5 6 5206.0 7 5092.5 8 4979.0 9 5217.0 10 5455.0 11 5652.0 12 5849.0 13 6042.0 14 6235.0 15 6150.5 16 6066.0 17 5973.5 18 5881.0 19 5918.5 20 5956.0 21 7323.5 22 8691.0 23 10336.0 24 11981.0 25 14500.0 26 26261.5 27 35504.0 28 37065.0 29 38626.0 30 46362.5 31 54099.0 32 65238.0 33 76377.0 34 85870.5 35 95364.0 36 103917.0 37 112470.0 38 131857.0 39 151244.0 40 162836.5 41 174429.0 42 198866.5 43 223304.0 44 257293.0 45 291282.0 46 315972.5 47 340663.0 48 320012.5 49 299362.0 50 268524.5 51 237687.0 52 223554.0 53 209421.0 54 212002.5 55 214584.0 56 215580.5 57 216577.0 58 204467.5 59 192358.0 60 162530.5 61 132703.0 62 114204.0 63 95705.0 64 77495.0 65 59285.0 66 48616.5 67 37948.0 68 32791.0 69 27634.0 70 22627.0 71 17620.0 72 13616.0 73 9612.0 74 7567.0 75 4278.0 76 3034.0 77 2081.5 78 1129.0 79 894.0 80 659.0 81 437.5 82 216.0 83 138.0 84 60.0 85 71.5 86 83.0 87 50.0 88 17.0 89 9.0 90 1.0 91 1.5 92 2.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3456687.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.979239532380332 #Duplication Level Percentage of deduplicated Percentage of total 1 69.99212640780948 10.48428826843221 2 11.429838090790573 3.4242056515655337 3 4.993705554048013 2.244057349447897 4 2.733312384727024 1.6377176371059117 5 1.8234005000072864 1.3656576426535603 6 1.2240785137298558 1.100145915815977 7 0.8915443938255992 0.934825992020513 8 0.6684811029818025 0.8010670851547379 9 0.5249693946080298 0.7077278078102132 >10 3.149325498233577 9.329655606002675 >50 0.7691105597883005 8.448882817181111 >100 1.7702029372968302 54.293329630477 >500 0.023108148027793162 2.185351668683255 >1k 0.005825583536418444 1.8521822554914806 >5k 7.767444715224592E-4 0.7835036942320265 >10k+ 1.941861178806148E-4 0.40740097792596397 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC 14006 0.4051856589850339 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8724 0.25238038619059233 No Hit ATCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG 6814 0.19712516638040992 No Hit ATGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT 6305 0.18240008424251314 No Hit CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT 5093 0.14733760968233456 Illumina Single End Adapter 1 (95% over 21bp) ATATCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC 4662 0.13486902343197404 No Hit CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC 3803 0.11001863923461974 No Hit ATGTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC 3792 0.10970041545560821 No Hit ATTGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC 3716 0.10750177843698316 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.1638563167564781 0.0 2 0.0 0.0 0.0 0.7474497980291533 0.0 3 0.0 0.0 0.0 1.03249151572011 0.0 4 0.0 0.0 0.0 1.416934770200484 0.0 5 0.0 0.0 0.0 2.6033019477898924 0.0 6 0.0 0.0 0.0 2.903849842349047 0.0 7 2.8929434455592884E-5 0.0 0.0 3.399266407401075 0.0 8 2.8929434455592884E-5 0.0 0.0 4.995679388964057 0.0 9 2.8929434455592884E-5 0.0 0.0 5.18563005559948 0.0 10 2.8929434455592884E-5 0.0 0.0 5.551240248249263 0.0 11 5.785886891118577E-5 0.0 0.0 5.836166248202398 0.0 12 5.785886891118577E-5 0.0 0.0 6.6863155385489055 0.0 13 5.785886891118577E-5 0.0 0.0 7.458471073603135 0.0 14 8.678830336677865E-5 0.0 0.0 7.787022660715303 0.0 15 8.678830336677865E-5 0.0 0.0 7.945064161146207 0.0 16 8.678830336677865E-5 0.0 0.0 8.041138812973232 0.0 17 1.446471722779644E-4 0.0 0.0 8.179971168925622 0.0 18 2.3143547564474307E-4 0.0 0.0 8.490036847420667 0.0 19 2.3143547564474307E-4 0.0 0.0 8.927941696774976 0.0 20 2.60364910100336E-4 0.0 0.0 9.402066198067688 0.0 21 2.60364910100336E-4 0.0 0.0 9.754629215777998 0.0 22 2.60364910100336E-4 0.0 0.0 10.11315169698616 0.0 23 3.182237790115217E-4 0.0 0.0 10.684826251263132 0.0 24 4.0501208237830037E-4 0.0 0.0 11.195430769404346 0.0 25 4.0501208237830037E-4 0.0 0.0 11.702968767493267 0.0 26 4.91800385745079E-4 0.0 0.0 12.10442252943353 0.0 27 5.20729820200672E-4 0.0 0.0 12.475124302547497 0.0 28 5.20729820200672E-4 0.0 0.0 12.809114623337317 0.0 29 5.496592546562648E-4 0.0 0.0 13.130752075614598 0.0 30 5.496592546562648E-4 0.0 0.0 13.46283305373035 0.0 31 5.496592546562648E-4 0.0 0.0 13.80908945472934 0.0 32 5.785886891118576E-4 0.0 0.0 14.18962723555821 0.0 33 6.364475580230434E-4 0.0 0.0 14.547223974863792 0.0 34 6.364475580230434E-4 0.0 0.0 14.903866042832343 0.0 35 6.653769924786364E-4 0.0 0.0 15.271964166845306 0.0 36 7.232358613898221E-4 0.0 0.0 15.639310125562425 0.0 37 7.232358613898221E-4 0.0 0.0 16.05355069753206 0.0 38 0.0015911188950576086 0.0 0.0 16.439874365252045 0.0 39 0.0015911188950576086 0.0 0.0 16.821569323459137 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACGTA 25 3.892437E-5 45.0 17 CGAACCG 25 3.892437E-5 45.0 17 ACACGAT 20 7.035255E-4 45.0 28 CGACGAA 45 3.8562575E-10 45.0 19 GACGTAT 20 7.035255E-4 45.0 26 GGCGTAA 20 7.035255E-4 45.0 9 CCGTAGT 20 7.035255E-4 45.0 17 CCGTAAG 25 3.892437E-5 45.0 30 CGTAACG 50 2.1827873E-11 45.0 3 CGTCGCA 25 3.892437E-5 45.0 22 AATAGCG 20 7.035255E-4 45.0 32 CGATTAC 30 2.166773E-6 44.999996 37 TCAATCG 60 0.0 44.999996 36 ATTTCGC 210 0.0 42.857143 1 ATTCGAT 280 0.0 42.589283 1 ATCGCTA 185 0.0 42.567566 1 TCCTACG 80 0.0 42.1875 44 TTACACG 295 0.0 41.949154 36 ATAGCGC 70 0.0 41.785713 1 TCGAGTA 65 0.0 41.538464 2 >>END_MODULE