FastQCFastQC Report
Thu 26 May 2016
SRR1545607_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545607_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2904594
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTT122870.4230195338832209Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT100060.344488765039107Illumina Single End Adapter 1 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC82690.2846869476422522No Hit
GTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC74370.25604266895820893No Hit
ATCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC71700.24685033433244027No Hit
TGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC67540.23252819499041863No Hit
GCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC49310.16976555071035745No Hit
AGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC42360.14583793810770113No Hit
GGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC37350.12858940010204525No Hit
TCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC36300.12497443704696767No Hit
ACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC36140.124423585533813No Hit
CTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC34560.11898392684141054No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT32080.11044572838751303Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA405.6170393E-946.00000417
GGCGTAA206.3130533E-446.0000047
TACGCCG206.3130533E-446.00000416
TCGCTAA206.3130533E-446.00000426
ACGCTTA206.3130533E-446.00000426
CCGCGTA351.02028935E-746.00000439
CGGTACC206.3130533E-446.00000434
TTCGATC206.3130533E-446.00000428
GTGCGTT206.3130533E-446.00000437
ATACCGG405.6170393E-946.0000042
TACGTCG206.3130533E-446.00000439
GACGTAT253.4184537E-546.024
CCCGTAT453.110472E-1046.019
TTCGATA253.4184537E-546.015
TACCGGT253.4184537E-546.040
TAGCGAT301.8623923E-645.99999619
CCGTCGA301.8623923E-645.99999635
TTATACG600.045.9999961
TCAATCG301.8623923E-645.99999634
GTTCGAC301.8623923E-645.99999646