##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545607_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2904594
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5361396463671	33.0	31.0	34.0	31.0	34.0
2	32.95070980660292	34.0	31.0	34.0	31.0	34.0
3	33.02747406350078	34.0	33.0	34.0	31.0	34.0
4	36.40531688766141	37.0	37.0	37.0	35.0	37.0
5	36.37747995072633	37.0	37.0	37.0	35.0	37.0
6	36.38915387141886	37.0	37.0	37.0	35.0	37.0
7	36.494774484833336	37.0	37.0	37.0	35.0	37.0
8	36.54403025001084	37.0	37.0	37.0	35.0	37.0
9	38.38487375516165	39.0	39.0	39.0	37.0	39.0
10	38.09160970517738	39.0	39.0	39.0	35.0	39.0
11	37.87960451615613	39.0	38.0	39.0	35.0	39.0
12	37.47321071378651	39.0	37.0	39.0	35.0	39.0
13	37.406487791409056	39.0	37.0	39.0	35.0	39.0
14	38.71215598462298	41.0	38.0	41.0	35.0	41.0
15	38.708729343928965	41.0	38.0	41.0	35.0	41.0
16	38.7514162048121	41.0	38.0	41.0	35.0	41.0
17	38.656300674035684	40.0	38.0	41.0	35.0	41.0
18	38.62587680068195	40.0	37.0	41.0	34.0	41.0
19	38.62314354433012	40.0	37.0	41.0	34.0	41.0
20	38.57662241263323	40.0	37.0	41.0	35.0	41.0
21	38.54801152932217	40.0	37.0	41.0	35.0	41.0
22	38.640071899893755	40.0	37.0	41.0	35.0	41.0
23	38.63803684783484	40.0	37.0	41.0	35.0	41.0
24	38.616842835866215	40.0	37.0	41.0	35.0	41.0
25	38.54217870036226	40.0	37.0	41.0	35.0	41.0
26	38.43771935079395	40.0	36.0	41.0	35.0	41.0
27	38.387757807115214	40.0	36.0	41.0	35.0	41.0
28	38.32376400970325	40.0	36.0	41.0	35.0	41.0
29	38.29324304877033	40.0	36.0	41.0	35.0	41.0
30	38.22274679352777	40.0	36.0	41.0	34.0	41.0
31	38.171788208610224	40.0	36.0	41.0	34.0	41.0
32	38.11155500562213	40.0	35.0	41.0	34.0	41.0
33	38.03221104223172	40.0	35.0	41.0	34.0	41.0
34	37.88585392657287	40.0	35.0	41.0	34.0	41.0
35	37.82223952814059	40.0	35.0	41.0	34.0	41.0
36	37.77191786528513	40.0	35.0	41.0	34.0	41.0
37	37.701044965320456	40.0	35.0	41.0	34.0	41.0
38	37.6509157562124	40.0	35.0	41.0	34.0	41.0
39	37.57468685812888	40.0	35.0	41.0	33.0	41.0
40	37.45991350254115	40.0	35.0	41.0	33.0	41.0
41	37.33050815363524	39.0	35.0	41.0	33.0	41.0
42	37.25576104612211	39.0	35.0	41.0	33.0	41.0
43	37.1981843245562	39.0	35.0	41.0	33.0	41.0
44	37.06791586018562	39.0	35.0	41.0	33.0	41.0
45	37.00552194213718	39.0	35.0	41.0	33.0	41.0
46	36.98528778893023	39.0	35.0	41.0	33.0	41.0
47	36.948101524688134	39.0	35.0	41.0	33.0	41.0
48	36.86274260705627	38.0	35.0	41.0	33.0	41.0
49	36.77269077881453	38.0	35.0	41.0	33.0	41.0
50	36.67892311283436	38.0	35.0	40.0	33.0	41.0
51	36.57484040798818	38.0	35.0	40.0	32.0	41.0
52	36.270768995598004	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	5.0
11	3.0
12	10.0
13	25.0
14	35.0
15	70.0
16	123.0
17	276.0
18	461.0
19	785.0
20	1271.0
21	1975.0
22	3130.0
23	4550.0
24	6348.0
25	8543.0
26	10956.0
27	14363.0
28	17886.0
29	23000.0
30	29355.0
31	37864.0
32	49683.0
33	69221.0
34	167507.0
35	333330.0
36	170695.0
37	239103.0
38	417681.0
39	1294294.0
40	2042.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.823193878387134	13.586580430862282	48.59030212139803	12.99992356935255
2	23.809523809523807	12.816076876837176	51.62339383748641	11.751005476152605
3	24.080232900019762	13.71475669232946	48.066373475948794	14.138636931701987
4	22.099164289398104	13.871611660700257	49.31959509659525	14.709628953306384
5	21.456045147790018	16.300832405492816	47.32885215627382	14.914270290443346
6	23.942726591048526	18.71187505035127	44.990246485395204	12.355151873205
7	87.29550498279622	2.010642451234148	6.678936884122187	4.014915681847446
8	89.58773584191113	1.8241103575921453	5.716599290640964	2.871554509855766
9	82.75270141024873	3.5625977331083103	7.837343188066903	5.8473576685760555
10	46.72029894711619	23.921725377109503	11.446969869110795	17.911005806663514
11	34.582251426533276	21.72041944588469	25.034445433681952	18.662883693900074
12	30.073187509166516	21.953773918144844	26.95323339509756	21.019805177591085
13	23.087529616875887	23.405611937503142	33.292467036701176	20.214391408919802
14	18.714147312843036	29.196300756663412	31.3586683715521	20.730883558941457
15	19.40143786016221	22.75243975577998	36.02634309648784	21.81977928756997
16	24.64946908242598	21.234602839501836	32.287197453413455	21.82873062465873
17	23.176939703104807	22.21601366662604	28.097386416139397	26.509660214129756
18	24.697014453655143	21.81585447053874	29.711519062560892	23.775612013245226
19	24.57393356868464	26.486317881259826	26.53007614833605	22.409672401719483
20	26.779887309551697	22.90106637967303	25.97591952610244	24.343126784672833
21	25.57142237434905	27.113427900766858	27.209207207616625	20.105942517267472
22	29.066196514900188	20.84325726762501	26.592081371785525	23.498464845689277
23	28.052182163841145	23.066666115815153	23.452847454756157	25.42830426558755
24	28.578865066856157	19.863430138601128	28.161044194128337	23.39666060041438
25	29.71193220119576	22.022148362215166	22.562843550596057	25.703075885993016
26	22.861783781141185	24.658282706636452	24.684723579267878	27.795209932954485
27	20.80259754031028	21.614552670700277	25.785187189672637	31.797662599316805
28	21.908225383650866	23.637279427004255	27.11635429943049	27.338140889914392
29	22.072620132107964	21.56773029208213	28.093082888692877	28.266566687117027
30	22.91917562316799	25.245765845415917	25.607262150923678	26.227796380492418
31	25.27282642599964	23.393665345311597	25.304500388006034	26.029007840682727
32	27.02284725507248	22.818197655162823	25.160280576218224	24.99867451354647
33	27.686244618008576	25.499570680101936	24.011961740608154	22.802222961281334
34	21.62209245078658	22.241318408011583	30.58558270105908	25.551006440142753
35	21.46702774983354	23.60415947977583	27.988765383389207	26.94004738700142
36	25.320268512570088	25.57293721601022	25.25079236547345	23.856001905946236
37	24.05764798798042	26.439151220445957	27.357041982459513	22.146158809114112
38	22.144161972378928	25.5936285759731	29.032732285476044	23.229477166171932
39	23.06866295255034	26.047426938153833	24.858758229205183	26.02515188009064
40	26.95939604640098	22.08763083584143	25.392120206817197	25.56085291094039
41	22.06797232246572	22.43821339574481	27.731517726745974	27.76229655504349
42	20.848869067415272	23.249514389962936	25.4015191107604	30.500097431861388
43	20.876755925268732	20.424437976529592	28.00467122083155	30.69413487737013
44	24.568115199576944	21.60315693002189	25.316584693075868	28.5121431773253
45	21.041391671262836	22.46685767442885	26.78949278281233	29.70225787149598
46	21.834858847742574	23.276540542327087	25.72617722132594	29.162423388604395
47	21.610042573936322	22.66994974168507	29.475479189174113	26.244528495204495
48	21.039050552331926	20.843945832016452	32.68377611466525	25.433227500986366
49	21.810862378700776	20.51315949836707	28.556590008792966	29.119388114139188
50	20.80748634748953	21.20509785532849	31.06578750765167	26.921628289530307
51	21.214083620636824	21.63861799618122	29.370335406600717	27.77696297658124
52	20.604738562429034	19.560427378146482	30.386140025077513	29.448694034346968
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	323.0
1	250.5
2	178.0
3	165.0
4	152.0
5	234.5
6	317.0
7	425.5
8	534.0
9	795.0
10	1056.0
11	1237.5
12	1419.0
13	1663.5
14	1695.0
15	1482.0
16	1756.5
17	2031.0
18	2259.5
19	2488.0
20	2724.5
21	2961.0
22	3684.0
23	4407.0
24	4949.5
25	5492.0
26	9264.0
27	13036.0
28	16471.5
29	19907.0
30	24005.5
31	28104.0
32	32779.5
33	37455.0
34	47191.0
35	56927.0
36	58111.0
37	59295.0
38	67418.5
39	90351.5
40	105161.0
41	119349.0
42	133537.0
43	149311.5
44	165086.0
45	190343.5
46	215601.0
47	257543.5
48	299486.0
49	305823.0
50	312160.0
51	275727.5
52	239295.0
53	218797.0
54	198299.0
55	191181.5
56	184064.0
57	173534.0
58	163004.0
59	161237.5
60	159471.0
61	144938.0
62	130405.0
63	111036.0
64	80897.5
65	70128.0
66	55527.5
67	40927.0
68	34486.5
69	28046.0
70	25573.0
71	23100.0
72	18518.5
73	13937.0
74	10623.0
75	7309.0
76	5703.5
77	4098.0
78	3224.5
79	2351.0
80	1729.0
81	1107.0
82	1026.5
83	946.0
84	569.0
85	192.0
86	137.0
87	82.0
88	65.5
89	39.0
90	29.0
91	25.5
92	22.0
93	15.5
94	9.0
95	8.5
96	8.0
97	5.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2904594.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.597552899690362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.61988661580384	10.235096527616838
2	12.800383166177554	3.9930930714152075
3	6.006195840980503	2.8104587206678087
4	3.369464281879357	2.1022158952092185
5	2.1131466678388557	1.6479958468210487
6	1.429019261350351	1.3373522114153127
7	1.0417536201450925	1.1374165039059911
8	0.7577651866709194	0.9455426067714725
9	0.5790422233305469	0.8128477538597273
>10	3.6060701765855026	11.409503417202602
>50	0.9453670146538633	10.926047800225236
>100	1.7124040049323714	47.910109851063005
>500	0.013961615886722976	1.4259247668833928
>1k	0.004210646061075184	1.5116143697415314
>5k	8.864518023316176E-4	1.0241964230280938
>10k+	4.432259011658088E-4	0.7705842341736516
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTT	12287	0.4230195338832209	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	10006	0.344488765039107	Illumina Single End Adapter 1 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	8269	0.2846869476422522	No Hit
GTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	7437	0.25604266895820893	No Hit
ATCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	7170	0.24685033433244027	No Hit
TGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	6754	0.23252819499041863	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	4931	0.16976555071035745	No Hit
AGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	4236	0.14583793810770113	No Hit
GGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	3735	0.12858940010204525	No Hit
TCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	3630	0.12497443704696767	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	3614	0.124423585533813	No Hit
CTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	3456	0.11898392684141054	No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	3208	0.11044572838751303	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4428219572167404E-5	0.0	0.0	0.4448125968724028	0.0
2	3.4428219572167404E-5	0.0	0.0	1.0476162933614819	0.0
3	3.4428219572167404E-5	0.0	0.0	3.212669309376801	0.0
4	3.4428219572167404E-5	0.0	0.0	3.7265104864913994	0.0
5	3.4428219572167404E-5	0.0	0.0	4.56363264538865	0.0
6	3.4428219572167404E-5	0.0	0.0	7.39855552961963	0.0
7	3.4428219572167404E-5	0.0	0.0	7.736916071574892	0.0
8	3.4428219572167404E-5	0.0	0.0	8.386783144219123	0.0
9	3.4428219572167404E-5	0.0	0.0	8.821921411391747	0.0
10	3.4428219572167404E-5	0.0	0.0	10.310459912813977	0.0
11	3.4428219572167404E-5	0.0	0.0	11.62430962812703	0.0
12	3.4428219572167404E-5	0.0	0.0	12.187383159229826	0.0
13	3.4428219572167404E-5	0.0	0.0	12.438812446765365	0.0
14	3.4428219572167404E-5	0.0	0.0	12.574012065025267	0.0
15	3.4428219572167404E-5	0.0	0.0	12.779720676968967	0.0
16	3.4428219572167404E-5	0.0	0.0	13.260682904392146	0.0
17	3.4428219572167404E-5	0.0	0.0	13.994003981279311	0.0
18	3.4428219572167404E-5	0.0	0.0	14.763646829815114	0.0
19	3.4428219572167404E-5	0.0	0.0	15.323690677595561	0.0
20	3.4428219572167404E-5	0.0	0.0	15.911345957472886	0.0
21	3.4428219572167404E-5	0.0	0.0	16.818632827858213	0.0
22	3.4428219572167404E-5	0.0	0.0	17.61936435866768	0.0
23	3.4428219572167404E-5	0.0	0.0	18.422368151968914	0.0
24	6.885643914433481E-5	0.0	0.0	19.046310775275305	0.0
25	6.885643914433481E-5	0.0	0.0	19.607146472105914	0.0
26	6.885643914433481E-5	0.0	0.0	20.103325972579988	0.0
27	6.885643914433481E-5	0.0	0.0	20.572548177129057	0.0
28	6.885643914433481E-5	0.0	0.0	21.053062837697798	0.0
29	6.885643914433481E-5	0.0	0.0	21.545420805799363	0.0
30	6.885643914433481E-5	0.0	0.0	22.082948597979616	0.0
31	6.885643914433481E-5	0.0	0.0	22.56339440210921	0.0
32	6.885643914433481E-5	0.0	0.0	23.048453587661477	0.0
33	6.885643914433481E-5	0.0	0.0	23.546147929796728	1.032846587165022E-4
34	6.885643914433481E-5	0.0	0.0	24.037679620628563	1.032846587165022E-4
35	6.885643914433481E-5	0.0	0.0	24.595554490575964	1.032846587165022E-4
36	7.918490501598502E-4	0.0	0.0	25.107054548759656	1.032846587165022E-4
37	7.918490501598502E-4	0.0	0.0	25.607158866264957	1.032846587165022E-4
38	7.918490501598502E-4	0.0	0.0	26.190854900891484	1.032846587165022E-4
39	7.918490501598502E-4	0.0	0.0	26.791317478449656	1.032846587165022E-4
40	0.0017558391981805374	0.0	0.0	27.41577652504963	1.032846587165022E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGAA	40	5.6170393E-9	46.000004	17
GGCGTAA	20	6.3130533E-4	46.000004	7
TACGCCG	20	6.3130533E-4	46.000004	16
TCGCTAA	20	6.3130533E-4	46.000004	26
ACGCTTA	20	6.3130533E-4	46.000004	26
CCGCGTA	35	1.02028935E-7	46.000004	39
CGGTACC	20	6.3130533E-4	46.000004	34
TTCGATC	20	6.3130533E-4	46.000004	28
GTGCGTT	20	6.3130533E-4	46.000004	37
ATACCGG	40	5.6170393E-9	46.000004	2
TACGTCG	20	6.3130533E-4	46.000004	39
GACGTAT	25	3.4184537E-5	46.0	24
CCCGTAT	45	3.110472E-10	46.0	19
TTCGATA	25	3.4184537E-5	46.0	15
TACCGGT	25	3.4184537E-5	46.0	40
TAGCGAT	30	1.8623923E-6	45.999996	19
CCGTCGA	30	1.8623923E-6	45.999996	35
TTATACG	60	0.0	45.999996	1
TCAATCG	30	1.8623923E-6	45.999996	34
GTTCGAC	30	1.8623923E-6	45.999996	46
>>END_MODULE