FastQCFastQC Report
Thu 26 May 2016
SRR1545606_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545606_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3756289
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86940.23145183983447493No Hit
ACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC65800.17517289005185702No Hit
GGGACTGTCTAGGGCACTCAGTGCCTCAAGTGGCAGGCAGCATAGAGAACA58440.15557908350502317No Hit
ATGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA47390.12616175166500768No Hit
ATCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG45370.12078410367253424No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT38750.10316032658828968Illumina Single End Adapter 1 (95% over 21bp)
ATGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT38700.10302721648946607No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAC207.0353324E-445.035
TACGACA207.0353324E-445.034
CCGCGTA207.0353324E-445.027
CAATCGA502.1827873E-1145.018
GTACGTA253.8925013E-545.028
TACGAAT3300.040.90909212
ATAGTTT19600.039.8341831
ATGCGAA2050.039.5121961
CTAAACG403.4610093E-739.37522
TCGACGA403.4610093E-739.3752
ATTGTTT62850.039.3078731
ATCGGGT1800.038.751
CGCTAGA356.252072E-638.5714325
TTGTCGA1350.038.3333362
ATGCGTT10100.038.3168331
ATGCGAT4700.038.297871
ATGCGTA5150.038.009711
CGGGTAT2150.037.674428
TCCGATT301.1404152E-437.4999962
CCGTAAG301.1404152E-437.4999963
Produced by FastQC (version 0.11.5)