##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545606_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3756289
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.829760436430742	31.0	31.0	34.0	31.0	34.0
2	32.500234673104224	33.0	31.0	34.0	31.0	34.0
3	32.48428888192575	34.0	31.0	34.0	31.0	34.0
4	35.98449187482646	37.0	35.0	37.0	35.0	37.0
5	35.75914020460087	37.0	35.0	37.0	35.0	37.0
6	35.76270888635033	37.0	35.0	37.0	35.0	37.0
7	35.73706921911493	37.0	35.0	37.0	33.0	37.0
8	35.70352014980743	37.0	35.0	37.0	33.0	37.0
9	37.876075829096216	39.0	38.0	39.0	35.0	39.0
10	37.832178514485975	39.0	38.0	39.0	35.0	39.0
11	37.64831566474252	39.0	37.0	39.0	35.0	39.0
12	37.18158320619101	39.0	37.0	39.0	33.0	39.0
13	36.89880357980975	39.0	37.0	39.0	33.0	39.0
14	37.45798020333366	39.0	35.0	41.0	33.0	41.0
15	37.34581577722055	40.0	35.0	41.0	32.0	41.0
16	37.06768302438923	40.0	35.0	41.0	31.0	41.0
17	37.228949902416986	40.0	35.0	41.0	31.0	41.0
18	37.345144369882085	39.0	35.0	41.0	32.0	41.0
19	37.31684037090863	39.0	35.0	41.0	32.0	41.0
20	37.28619469907667	39.0	35.0	41.0	32.0	41.0
21	37.26332851386036	39.0	35.0	41.0	32.0	41.0
22	37.17613314630477	39.0	35.0	41.0	31.0	41.0
23	37.1358939634304	39.0	35.0	41.0	31.0	41.0
24	37.16087100859385	39.0	35.0	41.0	32.0	41.0
25	37.11940055730536	39.0	35.0	41.0	32.0	41.0
26	37.02987975632333	39.0	35.0	41.0	31.0	41.0
27	36.94512935506294	39.0	35.0	41.0	31.0	41.0
28	36.851657047687226	39.0	35.0	41.0	31.0	41.0
29	36.77359436401193	39.0	35.0	41.0	31.0	41.0
30	36.6523906973079	38.0	35.0	41.0	31.0	41.0
31	36.52829028863328	38.0	35.0	41.0	30.0	41.0
32	36.370549497123356	38.0	35.0	41.0	30.0	41.0
33	36.184739779074505	38.0	35.0	41.0	30.0	41.0
34	36.17790564038071	38.0	35.0	41.0	30.0	41.0
35	36.08964246361236	38.0	35.0	41.0	30.0	41.0
36	35.87278641233409	38.0	34.0	40.0	29.0	41.0
37	35.778225796790394	38.0	34.0	40.0	29.0	41.0
38	35.715195769015644	38.0	34.0	40.0	29.0	41.0
39	35.661554528951314	38.0	34.0	40.0	29.0	41.0
40	35.59043406937006	38.0	34.0	40.0	29.0	41.0
41	35.47843762820166	37.0	34.0	40.0	28.0	41.0
42	35.34798467317078	37.0	34.0	40.0	28.0	41.0
43	35.20028890215848	37.0	34.0	40.0	27.0	41.0
44	35.131421996550316	37.0	34.0	40.0	27.0	41.0
45	35.10560050091993	36.0	34.0	40.0	27.0	41.0
46	35.03267852926119	36.0	34.0	40.0	27.0	41.0
47	34.94034484567082	36.0	34.0	40.0	27.0	41.0
48	34.87131368220071	36.0	34.0	40.0	27.0	41.0
49	34.820263563320076	35.0	34.0	40.0	27.0	41.0
50	34.69189777463875	35.0	34.0	40.0	27.0	41.0
51	34.4610308205785	35.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	11.0
10	34.0
11	49.0
12	44.0
13	55.0
14	77.0
15	155.0
16	315.0
17	670.0
18	1399.0
19	2542.0
20	4429.0
21	7244.0
22	11271.0
23	16429.0
24	22131.0
25	30658.0
26	40377.0
27	49425.0
28	58978.0
29	69978.0
30	84395.0
31	102850.0
32	129031.0
33	168005.0
34	288698.0
35	416679.0
36	290969.0
37	404555.0
38	591261.0
39	963187.0
40	384.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.2960427166280337	90.9197881206691	3.4904928774117225	3.293676285291148
2	3.7453188505996207	2.8100074302057165	90.74799622712736	2.6966774920673036
3	28.370048204491187	16.003374607225375	42.16765536411069	13.458921824172739
4	28.092513648443983	15.286470236981234	42.84137881829646	13.779637296278322
5	25.03199301225225	13.788156342603031	46.367598446232435	14.812252198912276
6	24.52875697263975	14.144492077153808	46.88539673065624	14.4413542195502
7	23.933036036364612	16.851552156929355	43.98583282596201	15.229578980744026
8	27.3132338858911	19.630704666227757	41.855805024586765	11.200256423294373
9	82.68754081488406	4.009515774744702	8.950828863274364	4.352114547096883
10	83.74334349673308	3.9354000717197213	8.614592753645953	3.706663677901248
11	77.37996197843138	6.355181936214173	10.154623352995468	6.110232732358986
12	48.079341073064406	19.87866215831636	15.258543738248044	16.7834530303712
13	38.23726023210674	20.895996021605367	22.284201242236687	18.582542504051204
14	31.795503487617697	20.107185575976715	27.702288082732718	20.395022853672867
15	27.97630853217098	19.740733473915345	32.1761185041939	20.106839489719775
16	21.896478146383306	24.84856729607333	33.9036746107661	19.351279946777257
17	22.632417260759222	19.47182445227191	37.21465520890432	20.68110307806455
18	29.778086829847222	18.565956985737785	31.000569977443167	20.65538620697183
19	28.82685544163402	19.791741263784548	28.03282708013148	23.348576214449952
20	29.62256099038173	19.39499330323093	29.872195669715506	21.110250036671832
21	28.591809629131305	23.257129576558143	26.897770645442883	21.253290148867674
22	28.028327958791245	23.797982530098192	26.650611813947222	21.523077697163345
23	28.855154648643914	23.160704620970325	28.21529440359887	19.76884632678689
24	29.750639527469797	19.059103279859457	28.685599004762413	22.504658187908333
25	29.63187869729938	20.444300212257364	26.576389622843184	23.347431467600067
26	30.359272143330823	20.556725001723773	27.07166567854603	22.012337176399367
27	31.697507832863764	19.28129065681581	24.06907455736233	24.952126952958093
28	25.5373055694064	21.783281318343718	25.399589861163506	27.279823251086377
29	24.64786388906711	20.52014634656705	28.644893936542154	26.187095827823686
30	24.353876924805306	22.255209862712906	27.586295942617834	25.804617269863954
31	26.574925411756123	23.138049282150547	25.63362403691516	24.653401269178172
32	29.947003545254375	21.99143889088406	26.702418264409367	21.3591392994522
33	29.15885865011984	21.270727571813563	27.539041857535455	22.031371920531143
34	31.641947677614795	20.30131334410105	24.331541050222707	23.725197928061444
35	32.179712476862136	21.380064206987267	24.39732406106133	22.042899255089267
36	25.737636268135915	22.3551223028899	29.043398950400253	22.863842478573932
37	25.70273480022437	25.116730901163354	28.136972421451066	21.04356187716121
38	28.449674665607468	26.490666719200785	23.025917334901546	22.0337412802902
39	27.09956555525946	26.8888522688217	23.982659481206053	22.028922694712787
40	27.25993660232213	24.181259748650863	25.52263683651604	23.036166812510963
41	25.435982162181876	24.760661386810227	23.883652189701056	25.919704261306837
42	28.350001823608356	21.062436889174396	25.83989144605221	24.74766984116504
43	24.351374454947422	22.3459909501106	25.90580756698965	27.39682702795232
44	22.53032181496152	23.092898336629585	26.63796635455898	27.73881349384991
45	22.587825377653317	20.803324770804377	27.85943786540386	28.749411986138444
46	25.66320110087376	20.954537843067985	26.648375564286987	26.733885491771264
47	23.706748868364492	21.80428609193808	26.48102954804596	28.00793549165147
48	23.32690589036147	22.388506315674856	26.844366873794854	27.440220920168816
49	23.789064153477007	22.484132610669732	29.041562031036484	24.685241204816773
50	23.981142026079464	23.040346469614025	28.85952065988533	24.11899084442118
51	25.112657732139354	21.289043521411692	27.959723013857563	25.638575732591395
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	999.0
1	2484.5
2	3970.0
3	9702.0
4	15434.0
5	10483.5
6	5533.0
7	5631.0
8	5729.0
9	5808.0
10	5887.0
11	6389.5
12	6892.0
13	6719.0
14	6546.0
15	6315.0
16	6084.0
17	6742.5
18	7401.0
19	8320.5
20	9240.0
21	9277.5
22	9315.0
23	11893.5
24	14472.0
25	15641.0
26	19898.5
27	22987.0
28	26592.5
29	30198.0
30	38346.5
31	46495.0
32	52449.0
33	58403.0
34	70199.0
35	81995.0
36	86399.5
37	90804.0
38	98891.5
39	106979.0
40	124438.0
41	141897.0
42	160321.0
43	178745.0
44	208706.0
45	238667.0
46	269647.0
47	300627.0
48	301629.0
49	302631.0
50	292930.0
51	283229.0
52	284713.0
53	286197.0
54	297794.0
55	309391.0
56	303640.5
57	297890.0
58	273043.0
59	248196.0
60	224910.5
61	201625.0
62	173557.5
63	145490.0
64	122424.0
65	99358.0
66	83343.0
67	67328.0
68	55939.5
69	44551.0
70	35772.0
71	26993.0
72	20681.5
73	14370.0
74	11362.5
75	6347.5
76	4340.0
77	3374.5
78	2409.0
79	1785.0
80	1161.0
81	760.5
82	360.0
83	252.0
84	144.0
85	96.5
86	49.0
87	43.0
88	37.0
89	25.0
90	13.0
91	16.5
92	20.0
93	30.5
94	41.0
95	21.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3756289.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.442858366699426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.14432911885365	12.027018440285591
2	10.166802294473229	3.343425803405161
3	3.9943841432129936	1.9703727818712384
4	2.1067377263953957	1.3856316020360744
5	1.2951633633500648	1.064809387265159
6	0.9024235731457063	0.8903053800003401
7	0.6668390504564802	0.7675318042028165
8	0.5300833962630147	0.6972868965833408
9	0.42634673288199887	0.6309323049495366
>10	3.6424084369700434	13.359077590859497
>50	1.5232422038412847	18.42679957975289
>100	1.584304904503397	41.566552475404706
>500	0.009444550267913595	1.034856431460209
>1k	0.0070019941641428374	2.269963606798578
>5k	4.885112207541515E-4	0.565435915124882
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8694	0.23145183983447493	No Hit
ACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	6580	0.17517289005185702	No Hit
GGGACTGTCTAGGGCACTCAGTGCCTCAAGTGGCAGGCAGCATAGAGAACA	5844	0.15557908350502317	No Hit
ATGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA	4739	0.12616175166500768	No Hit
ATCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG	4537	0.12078410367253424	No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	3875	0.10316032658828968	Illumina Single End Adapter 1 (95% over 21bp)
ATGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT	3870	0.10302721648946607	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6622019764719914E-5	0.0	0.0	0.11535321164053139	0.0
2	2.6622019764719914E-5	0.0	0.0	0.3978128413442097	0.0
3	2.6622019764719914E-5	0.0	0.0	0.5876810863061921	0.0
4	2.6622019764719914E-5	0.0	0.0	0.8103210375985447	0.0
5	2.6622019764719914E-5	0.0	0.0	1.6454804196375732	0.0
6	5.324403952943983E-5	0.0	0.0	1.8537178582372122	0.0
7	7.986605929415974E-5	0.0	0.0	2.2310583663823524	0.0
8	7.986605929415974E-5	0.0	0.0	3.398567043164144	0.0
9	7.986605929415974E-5	0.0	0.0	3.5990042299727203	0.0
10	7.986605929415974E-5	0.0	0.0	3.90318742780441	0.0
11	7.986605929415974E-5	0.0	0.0	4.160409382771134	0.0
12	1.0648807905887966E-4	0.0	0.0	4.888574867375753	0.0
13	1.5973211858831947E-4	0.0	0.0	5.497979521809957	0.0
14	1.863541383530394E-4	0.0	0.0	5.741224916400203	0.0
15	2.129761581177593E-4	0.0	0.0	5.8785945383861575	0.0
16	2.129761581177593E-4	0.0	0.0	6.030632893262473	0.0
17	2.662201976471991E-4	0.0	0.0	6.155090835662538	0.0
18	2.662201976471991E-4	0.0	0.0	6.387927020524779	0.0
19	2.9284221741191906E-4	0.0	0.0	6.677095399209167	0.0
20	3.1946423717663894E-4	0.0	0.0	6.995707731753334	0.0
21	3.460862569413589E-4	0.0	0.0	7.250560326960998	0.0
22	3.460862569413589E-4	0.0	0.0	7.541831845206799	0.0
23	3.993302964707987E-4	0.0	0.0	7.991344648934095	0.0
24	4.5257433600023856E-4	0.0	0.0	8.380744932032652	0.0
25	4.7919635576495844E-4	0.0	0.0	8.745599712908138	0.0
26	5.590624150591182E-4	0.0	0.0	9.05814222494595	0.0
27	6.123064545885581E-4	0.0	0.0	9.33479825434092	0.0
28	6.123064545885581E-4	0.0	0.0	9.584566043773522	0.0
29	6.123064545885581E-4	0.0	0.0	9.870193693829203	0.0
30	6.389284743532779E-4	0.0	0.0	10.131116109543221	0.0
31	6.655504941179978E-4	0.0	0.0	10.481461889646937	0.0
32	6.921725138827178E-4	0.0	0.0	10.888698925987857	0.0
33	7.454165534121576E-4	0.0	0.0	11.2167620755485	0.0
34	7.720385731768774E-4	0.0	0.0	11.576851514886101	0.0
35	7.986605929415974E-4	0.0	0.0	11.926957696811932	0.0
36	7.986605929415974E-4	0.0	0.0	12.32932290353591	0.0
37	8.252826127063173E-4	0.0	0.0	12.791694142809565	0.0
38	8.785266522357572E-4	0.0	0.0	13.147204594747635	0.0
39	9.317706917651969E-4	0.0	0.0	13.519034344801478	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCAC	20	7.0353324E-4	45.0	35
TACGACA	20	7.0353324E-4	45.0	34
CCGCGTA	20	7.0353324E-4	45.0	27
CAATCGA	50	2.1827873E-11	45.0	18
GTACGTA	25	3.8925013E-5	45.0	28
TACGAAT	330	0.0	40.909092	12
ATAGTTT	1960	0.0	39.834183	1
ATGCGAA	205	0.0	39.512196	1
CTAAACG	40	3.4610093E-7	39.375	22
TCGACGA	40	3.4610093E-7	39.375	2
ATTGTTT	6285	0.0	39.307873	1
ATCGGGT	180	0.0	38.75	1
CGCTAGA	35	6.252072E-6	38.57143	25
TTGTCGA	135	0.0	38.333336	2
ATGCGTT	1010	0.0	38.316833	1
ATGCGAT	470	0.0	38.29787	1
ATGCGTA	515	0.0	38.00971	1
CGGGTAT	215	0.0	37.67442	8
TCCGATT	30	1.1404152E-4	37.499996	2
CCGTAAG	30	1.1404152E-4	37.499996	3
>>END_MODULE