FastQCFastQC Report
Thu 26 May 2016
SRR1545605_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545605_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3163817
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCTT82540.26088740277961714Illumina Single End Adapter 1 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC65440.20683876469467102No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT59740.18882255199968898Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC53280.16840417761204265No Hit
GCCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC51190.16179823295721593No Hit
GGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC45070.14245450985312993No Hit
ATCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC44640.14109539205333305No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC43490.1374605421236437No Hit
TTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC42930.13569052824483843No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG36550.11552501298273572No Hit
TTGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAG33250.10509457405406192No Hit
AGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC31940.10095400587328535No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTA206.3131395E-446.00000428
TCGTATA206.3131395E-446.00000438
ACGTACT206.3131395E-446.00000437
TCGTTAC253.4185236E-546.026
ACCGTAA253.4185236E-546.019
TACGTAG453.110472E-1046.01
CGATGTA351.0203257E-745.99999634
TCTTACG554.7293724E-1141.818181
TACTATT13000.041.39999846
CGATAGT508.731149E-1041.39999824
TTTGTCG3350.040.507461
AACGTAT402.9127295E-740.25000440
CGCTTAT402.9127295E-740.25000422
ACTAATC18050.039.6288110
ATTATAC14050.039.61565842
TACGGGT1050.039.4285744
TCGATAG700.039.428571
CGTAGAA355.3761014E-639.4285720
GCGTAAT355.3761014E-639.4285724
TACCGTA355.3761014E-639.4285742
Produced by FastQC (version 0.11.5)