##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545605_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3163817
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52066096111121	33.0	31.0	34.0	31.0	34.0
2	32.93867186376456	34.0	31.0	34.0	31.0	34.0
3	33.01574458952588	34.0	33.0	34.0	31.0	34.0
4	36.410320508423844	37.0	37.0	37.0	35.0	37.0
5	36.37766248806426	37.0	37.0	37.0	35.0	37.0
6	36.385977444333854	37.0	37.0	37.0	35.0	37.0
7	36.461883541304694	37.0	37.0	37.0	35.0	37.0
8	36.50255435127885	37.0	37.0	37.0	35.0	37.0
9	38.32143420431713	39.0	39.0	39.0	37.0	39.0
10	38.074635795938896	39.0	39.0	39.0	35.0	39.0
11	37.861476185253444	39.0	38.0	39.0	35.0	39.0
12	37.34481261084317	39.0	37.0	39.0	35.0	39.0
13	37.208326840648496	39.0	36.0	39.0	35.0	39.0
14	38.09270131616336	40.0	37.0	41.0	33.0	41.0
15	38.29866582043146	40.0	36.0	41.0	33.0	41.0
16	38.45436856809354	40.0	36.0	41.0	34.0	41.0
17	38.42471641058886	40.0	36.0	41.0	34.0	41.0
18	38.40602506402867	40.0	36.0	41.0	34.0	41.0
19	38.35714170573077	40.0	36.0	41.0	34.0	41.0
20	38.22373734005475	40.0	36.0	41.0	33.0	41.0
21	38.224652374015314	40.0	36.0	41.0	34.0	41.0
22	38.40445828567202	40.0	36.0	41.0	34.0	41.0
23	38.43950108365939	40.0	36.0	41.0	35.0	41.0
24	38.3970134808682	40.0	36.0	41.0	35.0	41.0
25	38.31553120803131	40.0	35.0	41.0	35.0	41.0
26	38.184386454715934	40.0	35.0	41.0	34.0	41.0
27	38.079916126628056	40.0	35.0	41.0	34.0	41.0
28	37.96550717061069	40.0	35.0	41.0	34.0	41.0
29	37.89291826929307	40.0	35.0	41.0	34.0	41.0
30	37.797361225380605	40.0	35.0	41.0	34.0	41.0
31	37.647578225921414	40.0	35.0	41.0	34.0	41.0
32	37.63965204055734	40.0	35.0	41.0	34.0	41.0
33	37.57700935294298	40.0	35.0	41.0	33.0	41.0
34	37.34335993516692	40.0	35.0	41.0	33.0	41.0
35	37.27653242902481	39.0	35.0	41.0	33.0	41.0
36	37.208168171547214	39.0	35.0	41.0	33.0	41.0
37	37.11683798399212	39.0	35.0	41.0	33.0	41.0
38	37.05369495138309	39.0	35.0	41.0	33.0	41.0
39	36.90077080943683	39.0	35.0	41.0	32.0	41.0
40	36.771135625100946	39.0	35.0	41.0	32.0	41.0
41	36.58404958314593	38.0	35.0	41.0	32.0	41.0
42	36.517974016828404	38.0	35.0	41.0	32.0	41.0
43	36.448219350234226	38.0	35.0	40.0	32.0	41.0
44	36.32185963979585	38.0	35.0	40.0	31.0	41.0
45	36.25264577565643	37.0	35.0	40.0	31.0	41.0
46	36.22182920187862	37.0	35.0	40.0	31.0	41.0
47	36.16425286291843	37.0	35.0	40.0	31.0	41.0
48	36.031638682009735	37.0	35.0	40.0	31.0	41.0
49	35.91761091112413	37.0	35.0	40.0	31.0	41.0
50	35.803909328510464	36.0	35.0	40.0	31.0	41.0
51	35.677440888648114	36.0	35.0	40.0	31.0	41.0
52	35.315124420913094	36.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	7.0
11	21.0
12	28.0
13	55.0
14	92.0
15	113.0
16	214.0
17	405.0
18	702.0
19	1332.0
20	2319.0
21	3537.0
22	5157.0
23	7046.0
24	9870.0
25	12730.0
26	16044.0
27	19867.0
28	24695.0
29	30776.0
30	38430.0
31	49505.0
32	65277.0
33	95527.0
34	257965.0
35	334071.0
36	208216.0
37	308706.0
38	508620.0
39	1161129.0
40	1357.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.357555762548845	13.59117799796891	46.71110876514034	12.340157474341911
2	26.001440664867786	12.30428308590541	49.61930478279875	12.074971466428051
3	25.974953671467095	13.490761317737404	46.07617950090034	14.458105509895166
4	24.79574513949448	13.873463604247654	46.78358451199927	14.547206744258597
5	23.87476898948327	16.255459781649822	44.61026032795196	15.259510900914938
6	26.96255820105904	19.326907972237333	42.3268792095118	11.383654617191828
7	87.20434841838197	2.743047401287748	6.294769893454647	3.7578342868756316
8	88.5130524300236	2.4446736331462913	6.17987702828577	2.8623969085443313
9	81.6674921463536	4.254196750317734	8.230438106881657	5.847872996447013
10	50.50965337122849	18.636191663424277	13.293815666329625	17.56033929901761
11	40.06859435928184	19.52812062138866	21.809289222480313	18.593995796849185
12	33.42007454919169	19.706828808366602	26.010670022950126	20.862426619491583
13	29.542163785073537	18.505779569425158	32.235018649940876	19.71703799556043
14	21.750499475791425	24.30630469461413	34.265856716744366	19.677339112850078
15	22.26999854922077	18.900397842226653	37.523756905029586	21.30584670352299
16	30.43798045209315	18.812782155225793	29.667961200031478	21.081276192649575
17	29.976133259287753	19.760592979935314	26.67436201272071	23.588911748056223
18	30.770300557838837	18.887502026823928	28.57554656290171	21.766650852435525
19	29.034801949670285	23.565016560692353	26.052676245181054	21.3475052444563
20	28.03291087948513	24.192802554635744	25.206009070688978	22.568277495190145
21	28.37016173817892	23.462102896596104	28.04735545703181	20.120379908193172
22	29.791071986780526	18.805291203631562	28.66701835156711	22.736618458020804
23	29.70383558846798	20.31817895914966	25.80455822824139	24.173427224140966
24	30.644724394615746	20.61904971115586	25.453273688079936	23.28295220614846
25	33.99131492118539	19.079516925283606	21.626756541228524	25.302411612302482
26	25.52846767053847	21.293361784199274	24.610336185689626	28.56783435957263
27	25.21978989303111	18.818123804252902	27.87417856342513	28.087907739290863
28	24.671559701461874	21.25688685533961	26.635990640419465	27.435562802779046
29	26.973557573020184	22.720751547892938	23.913488043082136	26.392202836004735
30	31.528814719688274	21.165573103627676	25.608718835507872	21.696893341176178
31	28.854766252283238	20.300257568626755	28.127985910689524	22.71699026840048
32	33.13813662421057	19.29782917279982	23.130667797789823	24.433366405199795
33	34.275054467436014	20.047019154394835	23.312220649930133	22.36570572823902
34	25.78192733650524	21.575931856994256	29.366047404132413	23.27609340236809
35	25.795961017972914	24.301911267307812	28.489384815872725	21.412742898846552
36	29.403596984275637	25.243653473004287	22.606554045319307	22.746195497400766
37	28.01871916106399	26.8712760567378	23.020041930364492	22.089962851833718
38	27.766176109427317	22.6491924153641	26.36388261394385	23.22074886126473
39	25.764954167703124	23.58584583115901	24.13673104354645	26.512468957591416
40	29.918386556491733	19.54098482940069	26.222060251904583	24.318568362202996
41	25.074933221485313	21.58456067465343	25.465790214794342	27.87471588906691
42	22.510752044128974	22.568530354315687	26.67480451619041	28.245913085364926
43	22.548428053834975	19.55204741614322	28.315765418796346	29.58375911122546
44	27.66519049616334	19.29647005500002	25.593231214068325	27.445108234768316
45	23.951322089741602	19.88974077830671	26.37598824457925	29.782948887372434
46	23.59965826089183	20.93790506846635	26.553590172882945	28.908846497758876
47	24.064255296687513	21.124767962243077	29.86105074977472	24.94992599129469
48	24.05328753211706	22.068533040943898	29.744672337243273	24.13350708969577
49	25.52454835409254	19.470152666857786	27.709093161835845	27.296205817213824
50	22.508033808529383	21.005323632814413	28.91507315372539	27.57156940493082
51	24.495727787036987	21.81671695929316	27.40202736125383	26.285527892416027
52	23.206746787187754	21.170914752654785	27.630043077712774	27.992295382444688
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	161.0
1	136.0
2	111.0
3	112.0
4	113.0
5	185.0
6	257.0
7	472.0
8	687.0
9	813.5
10	940.0
11	1193.0
12	1446.0
13	1431.0
14	1352.0
15	1288.0
16	1713.0
17	2138.0
18	2139.5
19	2141.0
20	2225.5
21	2310.0
22	3030.0
23	3750.0
24	5283.0
25	6816.0
26	7961.0
27	9106.0
28	11645.0
29	14184.0
30	17215.5
31	20247.0
32	25346.5
33	30446.0
34	36374.5
35	42303.0
36	47193.0
37	52083.0
38	57137.0
39	68979.0
40	75767.0
41	85402.0
42	95037.0
43	108346.0
44	121655.0
45	149707.0
46	177759.0
47	216568.5
48	255378.0
49	277023.0
50	298668.0
51	285971.0
52	273274.0
53	261628.5
54	249983.0
55	251352.5
56	252722.0
57	247201.0
58	241680.0
59	234507.0
60	227334.0
61	202751.0
62	178168.0
63	159965.0
64	124170.0
65	106578.0
66	92732.0
67	78886.0
68	64946.5
69	51007.0
70	43270.0
71	35533.0
72	27645.5
73	19758.0
74	16627.5
75	13497.0
76	10222.0
77	6947.0
78	5329.0
79	3711.0
80	3049.0
81	2387.0
82	1681.0
83	975.0
84	848.5
85	722.0
86	466.5
87	211.0
88	167.0
89	87.0
90	51.0
91	37.5
92	24.0
93	22.0
94	20.0
95	29.0
96	38.0
97	29.0
98	20.0
99	14.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3163817.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.19060706065665
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.69462904568216	11.980929821625498
2	11.738849781281091	4.03595907868157
3	4.662402054869446	2.404485650521763
4	2.4632675072239527	1.6938025520788071
5	1.484236068383075	1.2757459518413676
6	1.01744165012103	1.0494263768626035
7	0.7461829489286173	0.8979136509275721
8	0.555672286344242	0.764187514323244
9	0.4381513746147825	0.6778879302680366
>10	4.073613388699022	15.815426135799818
>50	1.706894033859648	21.210206914646733
>100	1.405173630447368	34.563945633934914
>500	0.008682914912917878	1.029290422603938
>1k	0.0038796002802399024	1.6093262226174625
>5k	9.237143524380721E-4	0.9914661432666566
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCTT	8254	0.26088740277961714	Illumina Single End Adapter 1 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	6544	0.20683876469467102	No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	5974	0.18882255199968898	Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	5328	0.16840417761204265	No Hit
GCCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	5119	0.16179823295721593	No Hit
GGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	4507	0.14245450985312993	No Hit
ATCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	4464	0.14109539205333305	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	4349	0.1374605421236437	No Hit
TTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	4293	0.13569052824483843	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	3655	0.11552501298273572	No Hit
TTGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAG	3325	0.10509457405406192	No Hit
AGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	3194	0.10095400587328535	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2756796616239182	0.0
2	0.0	0.0	0.0	0.6165021554660083	0.0
3	0.0	0.0	0.0	2.1023971993323256	0.0
4	3.160739069295095E-5	0.0	0.0	2.4439150557696605	0.0
5	3.160739069295095E-5	0.0	0.0	3.0615234699099223	0.0
6	3.160739069295095E-5	0.0	0.0	5.04163167465122	0.0
7	3.160739069295095E-5	0.0	0.0	5.401418602909081	0.0
8	3.160739069295095E-5	0.0	0.0	5.9299573900766065	0.0
9	3.160739069295095E-5	0.0	0.0	6.330991963188769	0.0
10	3.160739069295095E-5	0.0	0.0	7.6086259097792315	0.0
11	3.160739069295095E-5	0.0	0.0	8.627079252687498	0.0
12	3.160739069295095E-5	0.0	0.0	9.043032514206732	0.0
13	6.32147813859019E-5	0.0	0.0	9.255054890975046	0.0
14	6.32147813859019E-5	0.0	0.0	9.433763077952992	0.0
15	6.32147813859019E-5	0.0	0.0	9.62397635514317	0.0
16	9.482217207885286E-5	0.0	0.0	9.986892415079634	0.0
17	9.482217207885286E-5	0.0	0.0	10.45588287818164	0.0
18	1.264295627718038E-4	0.0	0.0	10.953130348563144	0.0
19	1.264295627718038E-4	0.0	0.0	11.358684778544397	0.0
20	1.264295627718038E-4	0.0	0.0	11.838579791435471	0.0
21	1.264295627718038E-4	0.0	0.0	12.557711144481491	0.0
22	1.264295627718038E-4	0.0	0.0	13.188910736619722	0.0
23	1.264295627718038E-4	0.0	0.0	13.750984965312469	0.0
24	1.8964434415770571E-4	0.0	0.0	14.222756878795455	0.0
25	1.8964434415770571E-4	0.0	0.0	14.634885646040843	0.0
26	1.8964434415770571E-4	0.0	0.0	15.005103013227377	0.0
27	1.8964434415770571E-4	0.0	0.0	15.43249815017746	0.0
28	1.8964434415770571E-4	0.0	0.0	15.799428348731928	0.0
29	1.8964434415770571E-4	0.0	0.0	16.252709938659535	0.0
30	2.2125173485065667E-4	0.0	0.0	16.765034134401578	0.0
31	2.2125173485065667E-4	0.0	0.0	17.183357950222785	0.0
32	2.2125173485065667E-4	0.0	0.0	17.681079531464682	0.0
33	2.2125173485065667E-4	0.0	0.0	18.149058558064514	0.0
34	2.2125173485065667E-4	0.0	0.0	18.70149253259591	0.0
35	2.2125173485065667E-4	0.0	0.0	19.32226168580547	0.0
36	2.2125173485065667E-4	0.0	0.0	19.77706043048634	0.0
37	2.2125173485065667E-4	0.0	0.0	20.254426852121977	0.0
38	5.373256417801662E-4	0.0	0.0	20.936862024573482	0.0
39	5.373256417801662E-4	0.0	0.0	22.394563275941685	0.0
40	5.373256417801662E-4	0.0	0.0	22.984262364100072	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCGTA	20	6.3131395E-4	46.000004	28
TCGTATA	20	6.3131395E-4	46.000004	38
ACGTACT	20	6.3131395E-4	46.000004	37
TCGTTAC	25	3.4185236E-5	46.0	26
ACCGTAA	25	3.4185236E-5	46.0	19
TACGTAG	45	3.110472E-10	46.0	1
CGATGTA	35	1.0203257E-7	45.999996	34
TCTTACG	55	4.7293724E-11	41.81818	1
TACTATT	1300	0.0	41.399998	46
CGATAGT	50	8.731149E-10	41.399998	24
TTTGTCG	335	0.0	40.50746	1
AACGTAT	40	2.9127295E-7	40.250004	40
CGCTTAT	40	2.9127295E-7	40.250004	22
ACTAATC	1805	0.0	39.62881	10
ATTATAC	1405	0.0	39.615658	42
TACGGGT	105	0.0	39.428574	4
TCGATAG	70	0.0	39.42857	1
CGTAGAA	35	5.3761014E-6	39.42857	20
GCGTAAT	35	5.3761014E-6	39.42857	24
TACCGTA	35	5.3761014E-6	39.42857	42
>>END_MODULE