FastQCFastQC Report
Thu 26 May 2016
SRR1545603_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545603_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1765171
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCTT51000.2889238493041184Illumina Single End Adapter 1 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC39320.22275462263995952No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT33210.188140412458623Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC32820.18593099478747385No Hit
GCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC32800.1858176913171585No Hit
TTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC31630.17918943830371106No Hit
ATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC28640.16225056949156766No Hit
GGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC27670.15675535118127365No Hit
ACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC18320.10378597880885194No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCCG351.0199801E-746.00000441
GACGTAT206.312377E-446.00000412
ATTACGA206.312377E-446.00000441
CGATTAC206.312377E-446.00000437
ACTCGAT301.8619739E-646.012
GTATACG301.8619739E-646.01
TAATGCG253.417906E-546.01
GCGTAAT253.417906E-546.046
TTGTACG1400.042.7142871
CTATGCG601.8189894E-1242.1666681
TCGTAAG601.8189894E-1242.1666681
CCGATAG508.731149E-1041.39999811
CCGATCA451.589251E-840.88888538
CGGATAA1250.040.4811
ACGTAGG750.039.866672
TACGGGA6600.039.7272724
CGATTAG355.3747463E-639.4285741
TACGCGA355.3747463E-639.42857429
TCGTTAG1050.039.428571
TTGGGAC27500.038.4727255