##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545603_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1765171
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57543376817317	33.0	31.0	34.0	31.0	34.0
2	32.989915991141935	34.0	31.0	34.0	31.0	34.0
3	33.06743369339288	34.0	33.0	34.0	31.0	34.0
4	36.443137237128866	37.0	37.0	37.0	35.0	37.0
5	36.41928742314484	37.0	37.0	37.0	35.0	37.0
6	36.427990829217116	37.0	37.0	37.0	35.0	37.0
7	36.505360103921944	37.0	37.0	37.0	35.0	37.0
8	36.54397959177893	37.0	37.0	37.0	35.0	37.0
9	38.35167187768211	39.0	39.0	39.0	37.0	39.0
10	38.12182049217895	39.0	39.0	39.0	37.0	39.0
11	37.94739376524994	39.0	38.0	39.0	35.0	39.0
12	37.580400992311795	39.0	37.0	39.0	35.0	39.0
13	37.50446160740234	39.0	37.0	39.0	35.0	39.0
14	38.630196167963334	41.0	38.0	41.0	35.0	41.0
15	38.76805476636541	41.0	38.0	41.0	35.0	41.0
16	38.859790354588874	41.0	38.0	41.0	35.0	41.0
17	38.82035451522827	41.0	38.0	41.0	35.0	41.0
18	38.800185930994786	40.0	38.0	41.0	35.0	41.0
19	38.790252615752244	40.0	38.0	41.0	35.0	41.0
20	38.673514350734294	40.0	38.0	41.0	35.0	41.0
21	38.63960262206891	40.0	38.0	41.0	35.0	41.0
22	38.790612920787844	40.0	38.0	41.0	35.0	41.0
23	38.802803807676426	40.0	38.0	41.0	35.0	41.0
24	38.741777425529875	40.0	38.0	41.0	35.0	41.0
25	38.66637793165648	40.0	38.0	41.0	35.0	41.0
26	38.56082441871071	40.0	37.0	41.0	35.0	41.0
27	38.49014967954946	40.0	37.0	41.0	35.0	41.0
28	38.42811319696505	40.0	37.0	41.0	35.0	41.0
29	38.36732758469293	40.0	37.0	41.0	35.0	41.0
30	38.300101803168076	40.0	36.0	41.0	34.0	41.0
31	38.21363142721017	40.0	36.0	41.0	34.0	41.0
32	38.17060556739262	40.0	36.0	41.0	34.0	41.0
33	38.09101271208285	40.0	36.0	41.0	34.0	41.0
34	37.93549406828007	40.0	35.0	41.0	34.0	41.0
35	37.87487217952255	40.0	35.0	41.0	34.0	41.0
36	37.78490922409217	40.0	35.0	41.0	34.0	41.0
37	37.67777116211404	40.0	35.0	41.0	33.0	41.0
38	37.58823649380145	40.0	35.0	41.0	33.0	41.0
39	37.46861918760279	40.0	35.0	41.0	33.0	41.0
40	37.36600363364229	40.0	35.0	41.0	33.0	41.0
41	37.198675369128544	39.0	35.0	41.0	33.0	41.0
42	37.12769074497598	39.0	35.0	41.0	33.0	41.0
43	37.073102266012754	39.0	35.0	41.0	33.0	41.0
44	36.95387698982138	39.0	35.0	41.0	33.0	41.0
45	36.87600011556954	39.0	35.0	41.0	32.0	41.0
46	36.841893504935214	39.0	35.0	41.0	32.0	41.0
47	36.79388059287174	38.0	35.0	41.0	32.0	41.0
48	36.687435948131935	38.0	35.0	41.0	32.0	41.0
49	36.585917738281445	38.0	35.0	40.0	32.0	41.0
50	36.48744852481714	38.0	35.0	40.0	32.0	41.0
51	36.36977493965174	38.0	35.0	40.0	32.0	41.0
52	36.03464876773978	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	3.0
11	9.0
12	17.0
13	21.0
14	41.0
15	71.0
16	106.0
17	175.0
18	311.0
19	576.0
20	964.0
21	1505.0
22	2086.0
23	2974.0
24	4129.0
25	5358.0
26	6859.0
27	8882.0
28	10907.0
29	13935.0
30	17971.0
31	23169.0
32	30219.0
33	44621.0
34	109672.0
35	159313.0
36	109858.0
37	158739.0
38	278037.0
39	773567.0
40	1071.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.942375554549674	13.513874859716141	47.114188936935854	12.429560648798333
2	25.062840937223648	12.73814265020216	50.22312285891849	11.975893553655709
3	25.615478613686722	13.853955225867637	45.673648615346615	14.85691754509903
4	23.416428210071434	14.537968276161347	46.98009427981765	15.065509233949573
5	22.708111565395082	17.027358822459693	44.69969198451595	15.564837627629277
6	25.811097055186156	18.582675559478375	43.831164232813705	11.775063152521767
7	88.18822652309606	2.3835651050238194	5.74924469074101	3.678963681139108
8	89.25628168602361	2.4337018906383574	5.839490904847179	2.470525518490843
9	82.20263079327725	4.722545294478552	7.443358178895982	5.6314657333482145
10	43.87178352692175	24.894302025129576	13.269705881186583	17.96420856676209
11	35.25618764414326	22.265208299932414	24.231023509903572	18.247580546020753
12	29.057128176250345	22.750373759822704	26.6250125341964	21.567485529730547
13	25.887350290708376	22.519064725173934	32.41125080799537	19.18233417612231
14	19.92316891677917	28.508229514307683	32.53163574520542	19.03696582370773
15	20.898598492723934	21.77947632269055	36.24696984031575	21.07495534426976
16	26.64166814433276	22.259826385092435	29.917441426354728	21.181064044220076
17	26.475508605115312	21.03393948801561	27.298205103075002	25.19234680379408
18	26.710556654284485	20.745355549122436	29.00523518684592	23.538852609747156
19	27.050070503084406	23.786477344121334	27.057945094271318	22.105507058522942
20	26.661496251637946	24.7031590707076	25.238461316212423	23.396883361442036
21	27.60072536881696	23.780812170605568	28.191602966511457	20.42685949406601
22	29.056505007163615	19.328665608034576	26.949457021444385	24.66537236335743
23	28.497918898508985	22.410859911022783	25.24656251433997	23.84465867612826
24	27.607296970095245	22.21966030486565	26.883684356926324	23.28935836811278
25	30.362270850812756	22.365481871161492	23.92714360251783	23.345103675507925
26	24.30110170629361	25.20486683726392	24.06865963694169	26.425371819500775
27	23.101784473005733	21.889720599307374	28.75789371114753	26.25060121653936
28	23.28760216432289	23.009045582552627	29.001722779266142	24.70162947385834
29	23.32402923002927	24.90693536206974	26.78924591441849	24.979789493482503
30	27.854241883647536	23.076857709536355	27.313727678508204	21.755172728307908
31	26.377614406762856	23.66643231732223	26.11673316636179	23.839220109553125
32	29.949846218864916	23.77004834092561	23.73894653832405	22.541158901885428
33	30.798885773672918	21.503808979413325	24.018522851327152	23.6787823955866
34	23.34521697897824	23.017599994561433	28.33334560787595	25.303837418584372
35	25.913183481940276	23.77021829613108	28.53083355663559	21.78576466529305
36	28.170471869297646	24.220259680223617	24.655741568380627	22.953526882098107
37	26.12398458846197	25.453964516752205	25.710143663135188	22.711907231650645
38	26.699849476339686	22.952393847394955	26.878642352497295	23.469114323768064
39	26.009264824767687	23.668755038463697	23.708467904809222	26.613512231959398
40	28.548452246269623	21.366995039007552	27.442157162110643	22.642395552612182
41	24.793461936548926	22.013334685421412	27.46226852809161	25.730934849938052
42	22.92049892050119	24.013820757309066	27.512235358500675	25.55344496368907
43	23.219676733868845	21.097955948743778	28.656713712155934	27.025653605231447
44	26.426958068085188	22.276028781347527	26.11763959412431	25.179373556442975
45	23.091643812412507	22.387009530521407	27.24194993006343	27.279396727002652
46	23.67226744604347	22.583024534166945	26.24980809224715	27.49489992754243
47	24.14791541442727	23.129260564557203	29.517026962260314	23.205797058755216
48	24.09324649000012	22.99726202163983	29.910020049049074	22.999471439310977
49	24.797257602804486	21.95521000514964	26.62665543451598	26.620876957529894
50	23.37156003582656	22.287755690525167	28.281735877147312	26.058948396500963
51	25.032872169325238	22.8053825946608	26.544000552920938	25.617744683093026
52	23.823980792795712	21.51128700845414	27.359502280515596	27.305229918234552
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	130.0
1	101.5
2	73.0
3	71.0
4	69.0
5	110.5
6	152.0
7	199.5
8	247.0
9	317.5
10	388.0
11	515.5
12	643.0
13	702.5
14	830.5
15	899.0
16	1033.0
17	1167.0
18	1114.5
19	1062.0
20	1449.5
21	1837.0
22	2601.5
23	3366.0
24	5266.0
25	7166.0
26	8320.5
27	9475.0
28	10655.5
29	11836.0
30	15265.0
31	18694.0
32	20118.0
33	21542.0
34	26204.0
35	30866.0
36	35155.5
37	39445.0
38	42159.5
39	52421.0
40	59968.0
41	70339.0
42	80710.0
43	89716.0
44	98722.0
45	108802.0
46	118882.0
47	139898.5
48	160915.0
49	166879.0
50	172843.0
51	161375.5
52	149908.0
53	136903.0
54	123898.0
55	119924.0
56	115950.0
57	112225.0
58	108500.0
59	100826.5
60	93153.0
61	87553.0
62	81953.0
63	70430.5
64	53414.5
65	47921.0
66	40726.0
67	33531.0
68	28500.0
69	23469.0
70	19573.0
71	15677.0
72	13263.5
73	10850.0
74	8454.5
75	6059.0
76	5114.0
77	4169.0
78	3272.5
79	2376.0
80	1762.5
81	1149.0
82	829.5
83	510.0
84	318.5
85	127.0
86	142.5
87	158.0
88	103.0
89	35.5
90	23.0
91	22.5
92	22.0
93	20.0
94	18.0
95	22.0
96	26.0
97	17.5
98	9.0
99	17.5
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1765171.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.24054966403589
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.60377714300856	12.878517034455694
2	11.566416702826189	4.219555966056706
3	4.652559188513946	2.545957108288654
4	2.3086876328716217	1.6844692570456103
5	1.2928667971752081	1.17913005114287
6	0.9099309080459304	0.995858395143184
7	0.6364360073945373	0.8126259820602537
8	0.4845463191980679	0.7070712959886515
9	0.39462331954364566	0.6478331632850314
>10	4.074638468264564	17.85846230324621
>50	1.874446065205008	24.99992631285097
>100	1.1929959740335272	28.929592792979307
>500	0.003416546658121271	0.40496466995477065
>1k	0.004348332110336163	1.8470989276070775
>5k	3.105951507382974E-4	0.28893673989498186
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCTT	5100	0.2889238493041184	Illumina Single End Adapter 1 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	3932	0.22275462263995952	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT	3321	0.188140412458623	Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	3282	0.18593099478747385	No Hit
GCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	3280	0.1858176913171585	No Hit
TTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	3163	0.17918943830371106	No Hit
ATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	2864	0.16225056949156766	No Hit
GGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	2767	0.15675535118127365	No Hit
ACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	1832	0.10378597880885194	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3045597282076354	0.0
2	0.0	0.0	0.0	0.6543841928062494	0.0
3	0.0	0.0	0.0	2.3225511862590085	0.0
4	0.0	0.0	0.0	2.669373108894266	0.0
5	5.665173515767028E-5	0.0	0.0	3.3051755325687995	0.0
6	5.665173515767028E-5	0.0	0.0	5.2268590408521325	0.0
7	5.665173515767028E-5	0.0	0.0	5.485020997965636	0.0
8	5.665173515767028E-5	0.0	0.0	5.956816648358714	0.0
9	5.665173515767028E-5	0.0	0.0	6.288285950766243	0.0
10	5.665173515767028E-5	0.0	0.0	7.386706443738312	0.0
11	5.665173515767028E-5	0.0	0.0	8.350862324386702	0.0
12	5.665173515767028E-5	0.0	0.0	8.736377382134648	0.0
13	1.1330347031534056E-4	0.0	0.0	8.939813763085843	0.0
14	1.1330347031534056E-4	0.0	0.0	9.06048195897168	0.0
15	1.1330347031534056E-4	0.0	0.0	9.239501442069919	0.0
16	1.1330347031534056E-4	0.0	0.0	9.640595726986223	0.0
17	1.1330347031534056E-4	0.0	0.0	10.151990940254514	0.0
18	1.1330347031534056E-4	0.0	0.0	10.69851022932056	0.0
19	1.1330347031534056E-4	0.0	0.0	11.08266564542472	0.0
20	1.1330347031534056E-4	0.0	0.0	11.529421228878109	0.0
21	1.6995520547301082E-4	0.0	0.0	12.179896451958479	0.0
22	1.6995520547301082E-4	0.0	0.0	12.763409324082483	0.0
23	1.6995520547301082E-4	0.0	0.0	13.324318153878576	0.0
24	1.6995520547301082E-4	0.0	0.0	13.747789874182162	0.0
25	1.6995520547301082E-4	0.0	0.0	14.146958000103107	0.0
26	1.6995520547301082E-4	0.0	0.0	14.520292934792153	0.0
27	1.6995520547301082E-4	0.0	0.0	14.932207701123573	0.0
28	1.6995520547301082E-4	0.0	0.0	15.287414080562167	0.0
29	1.6995520547301082E-4	0.0	0.0	15.71966681981519	0.0
30	1.6995520547301082E-4	0.0	0.0	16.172427487195293	0.0
31	1.6995520547301082E-4	0.0	0.0	16.562418032020695	0.0
32	1.6995520547301082E-4	0.0	0.0	16.973086460178646	0.0
33	1.6995520547301082E-4	0.0	0.0	17.384774619569434	0.0
34	1.6995520547301082E-4	0.0	0.0	17.84642960936929	0.0
35	1.6995520547301082E-4	0.0	0.0	18.333181317843994	0.0
36	1.6995520547301082E-4	0.0	0.0	18.736031806550187	0.0
37	1.6995520547301082E-4	0.0	0.0	19.16290263096323	0.0
38	1.6995520547301082E-4	0.0	0.0	19.666196646103977	0.0
39	1.6995520547301082E-4	0.0	0.0	20.57772306479089	0.0
40	1.6995520547301082E-4	0.0	0.0	21.026234852034165	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATCCG	35	1.0199801E-7	46.000004	41
GACGTAT	20	6.312377E-4	46.000004	12
ATTACGA	20	6.312377E-4	46.000004	41
CGATTAC	20	6.312377E-4	46.000004	37
ACTCGAT	30	1.8619739E-6	46.0	12
GTATACG	30	1.8619739E-6	46.0	1
TAATGCG	25	3.417906E-5	46.0	1
GCGTAAT	25	3.417906E-5	46.0	46
TTGTACG	140	0.0	42.714287	1
CTATGCG	60	1.8189894E-12	42.166668	1
TCGTAAG	60	1.8189894E-12	42.166668	1
CCGATAG	50	8.731149E-10	41.399998	11
CCGATCA	45	1.589251E-8	40.888885	38
CGGATAA	125	0.0	40.48	11
ACGTAGG	75	0.0	39.86667	2
TACGGGA	660	0.0	39.727272	4
CGATTAG	35	5.3747463E-6	39.428574	1
TACGCGA	35	5.3747463E-6	39.428574	29
TCGTTAG	105	0.0	39.42857	1
TTGGGAC	2750	0.0	38.472725	5
>>END_MODULE