##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545602_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	849551
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89871473284123	31.0	31.0	34.0	31.0	34.0
2	32.57786171754256	34.0	31.0	34.0	31.0	34.0
3	32.51823492644938	34.0	31.0	34.0	31.0	34.0
4	35.99682302769345	37.0	35.0	37.0	35.0	37.0
5	35.75893619099972	37.0	35.0	37.0	35.0	37.0
6	35.759912000574424	37.0	35.0	37.0	35.0	37.0
7	35.74056766456634	37.0	35.0	37.0	33.0	37.0
8	35.700013301143784	37.0	35.0	37.0	33.0	37.0
9	37.92660946782477	39.0	38.0	39.0	35.0	39.0
10	37.91646999414985	39.0	38.0	39.0	35.0	39.0
11	37.71118861610427	39.0	38.0	39.0	35.0	39.0
12	37.210972619654385	39.0	37.0	39.0	33.0	39.0
13	36.85994719563628	39.0	35.0	39.0	33.0	39.0
14	37.06415506544045	39.0	35.0	41.0	32.0	41.0
15	36.70468047239071	39.0	35.0	41.0	31.0	41.0
16	36.79935872007684	39.0	35.0	41.0	31.0	41.0
17	37.0381578033573	39.0	35.0	41.0	32.0	41.0
18	37.12641618925762	39.0	35.0	41.0	32.0	41.0
19	37.10893165919409	38.0	35.0	41.0	32.0	41.0
20	37.10848083281639	38.0	35.0	41.0	32.0	41.0
21	37.09615432151807	38.0	35.0	41.0	32.0	41.0
22	36.9805544340481	38.0	35.0	41.0	32.0	41.0
23	36.898701784825164	38.0	35.0	41.0	32.0	41.0
24	36.868672981374864	38.0	35.0	41.0	32.0	41.0
25	36.8288637174225	38.0	35.0	41.0	32.0	41.0
26	36.73532018678102	38.0	35.0	41.0	32.0	41.0
27	36.63736373684452	38.0	35.0	41.0	31.0	41.0
28	36.490380212606425	38.0	35.0	41.0	31.0	41.0
29	36.36323540317179	38.0	35.0	41.0	31.0	41.0
30	36.31394701436406	38.0	35.0	41.0	31.0	41.0
31	36.23572804928721	38.0	35.0	40.0	31.0	41.0
32	36.14547213763505	38.0	35.0	40.0	30.0	41.0
33	35.97579074122684	37.0	35.0	40.0	30.0	41.0
34	35.86373978725233	37.0	35.0	40.0	30.0	41.0
35	35.789378153871866	37.0	35.0	40.0	30.0	41.0
36	35.71010804530864	37.0	35.0	40.0	30.0	41.0
37	35.51371018337922	37.0	34.0	40.0	29.0	41.0
38	35.45093231601163	37.0	34.0	40.0	29.0	41.0
39	35.366674867076846	37.0	34.0	40.0	29.0	41.0
40	35.30404531334788	36.0	34.0	40.0	28.0	41.0
41	35.31883547897654	36.0	34.0	40.0	29.0	41.0
42	35.135040745052386	36.0	34.0	40.0	28.0	41.0
43	35.0851897060918	36.0	34.0	40.0	28.0	41.0
44	34.98918134402761	36.0	34.0	40.0	28.0	41.0
45	34.94046149083457	36.0	34.0	40.0	28.0	41.0
46	34.84207422509066	35.0	34.0	40.0	27.0	41.0
47	34.74141281688798	35.0	34.0	40.0	27.0	41.0
48	34.699764934653714	35.0	34.0	40.0	27.0	41.0
49	34.65360878864247	35.0	34.0	40.0	27.0	41.0
50	34.55937195059508	35.0	34.0	39.0	27.0	41.0
51	34.38933389519876	35.0	34.0	39.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	2.0
11	10.0
12	9.0
13	10.0
14	14.0
15	24.0
16	42.0
17	120.0
18	248.0
19	464.0
20	835.0
21	1434.0
22	2311.0
23	3506.0
24	4967.0
25	7213.0
26	9753.0
27	11705.0
28	13348.0
29	15717.0
30	18734.0
31	23219.0
32	29283.0
33	38836.0
34	65507.0
35	149347.0
36	59211.0
37	77569.0
38	109614.0
39	206377.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.8864082321131987	91.72468751140308	2.5994907898407513	3.789413466642968
2	3.0743298518864672	2.43834684439192	91.72598231301005	2.761340990711564
3	26.51541814440805	15.784220135106663	44.54400030133564	13.156361419149645
4	26.126271406896112	15.879446907837199	43.881532715516784	14.112748969749903
5	22.96401275497292	15.436860176728647	47.75604996050855	13.843077107789881
6	20.798869049650932	14.64361762860617	49.44235248972693	15.11516083201597
7	22.491881005378133	17.37776778557144	46.23960186027678	13.890749348773646
8	24.86478151399975	18.757790880123736	44.22806870923582	12.149358896640695
9	83.30482807977391	3.79706456704777	8.905174615767624	3.992932737410703
10	85.28940581554257	3.30810039656242	8.297559534389343	3.104934253505675
11	78.96912604422806	5.901352596842332	9.0955104519917	6.0340109069379
12	50.5915477705282	19.61071201140367	14.41161272248517	15.38612749558296
13	41.741225659201156	21.625658730317543	21.48711495837213	15.146000652109173
14	34.754711606483895	23.99149668471934	24.048350246188868	17.205441462607894
15	21.287833220136285	34.471032345321234	29.233559845141727	15.007574589400754
16	15.224630422423138	38.89254441463785	31.465797815551984	14.417027347387032
17	15.31550195338479	21.207084683556374	46.27185419121395	17.205559171844893
18	18.619247108178318	19.385534241028495	42.71114977205606	19.28406887873712
19	19.292779362274896	18.49588782780551	29.32195948212644	32.88937332779315
20	23.155290265092972	20.51518978848827	34.23596699903831	22.09355294738044
21	28.025274527367987	22.41784189530705	29.757365949778176	19.799517627546788
22	29.538779896674832	21.5152474660144	25.120681395231127	23.82529124207964
23	23.30725289005604	27.431666845192343	28.338969643964873	20.922110620786746
24	27.659669637255448	20.899981284231316	25.561973324732712	25.878375753780524
25	24.37146210174551	27.16705648042319	23.102791945392333	25.35868947243897
26	24.753899412748616	20.268000390794665	34.76448147315464	20.213618723302073
27	24.65172779503526	21.932526711168606	31.174820581695506	22.240924912100628
28	19.584345142316355	29.77772964777865	27.464625431551493	23.173299778353506
29	21.35951814546743	29.616585702329818	26.680917331625764	22.342978820576988
30	20.9470649790301	26.297185219015688	34.0316237636116	18.724126038342607
31	18.964723718764382	24.943058156602724	32.01703017240872	24.075187952224177
32	22.37534886075115	28.43560892753937	28.62229577741654	20.566746434292938
33	30.210428802979454	23.19401660406497	24.82558433807976	21.769970254875812
34	29.099842151913187	25.6783877601227	25.35245088287813	19.86931920508598
35	28.73023514774275	25.238626050702077	24.2287985065052	21.802340295049973
36	23.97489968230277	24.172651200457654	27.453678472510774	24.398770644728803
37	22.88667778626592	25.931227201192158	30.253039546772353	20.929055465769565
38	29.148102939081937	24.377347563595357	24.007152013239935	22.467397484082767
39	23.382939929445083	30.292942978114322	26.752719966193904	19.571397126246687
40	22.451624446325177	31.306772636369097	24.286476032633708	21.955126884672023
41	22.747780886609515	29.586569846895593	23.49911894636108	24.166530320133813
42	24.729886728401237	23.990908138534355	24.58675229621294	26.692452836851466
43	21.064656506790058	22.76343621513011	26.153579949879408	30.018327328200428
44	21.895801429225557	23.06806772047823	26.020803930546844	29.015326919749374
45	22.02092634815332	21.636487980121263	27.37704975922576	28.96553591249966
46	21.58328340499864	26.88408347468251	27.287708448345064	24.244924671973784
47	20.559919298547115	31.33773016569929	24.103438169103445	23.99891236665015
48	21.108444342952925	27.357039188936273	24.58722313316093	26.947293334949872
49	22.70670036289758	24.246925729002733	29.03557290851285	24.01080099958684
50	24.382291351549227	22.70316908578767	31.122793098942854	21.791746463720248
51	21.853661522380648	22.65019992913904	28.929046049030603	26.567092499449707
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	602.0
1	677.0
2	752.0
3	2379.5
4	4007.0
5	2787.5
6	1568.0
7	1560.5
8	1553.0
9	1716.0
10	1879.0
11	1959.5
12	2040.0
13	2111.5
14	2183.0
15	2259.0
16	2335.0
17	2067.0
18	1799.0
19	2170.0
20	2541.0
21	2804.5
22	3068.0
23	3349.5
24	3631.0
25	4419.0
26	7186.0
27	9165.0
28	8389.0
29	7613.0
30	9121.0
31	10629.0
32	12532.0
33	14435.0
34	15791.5
35	17148.0
36	21259.0
37	25370.0
38	35202.5
39	45035.0
40	54780.0
41	64525.0
42	69712.5
43	74900.0
44	75833.5
45	76767.0
46	80240.5
47	83714.0
48	85347.0
49	86980.0
50	80733.0
51	74486.0
52	63278.0
53	52070.0
54	45778.0
55	39486.0
56	37150.0
57	34814.0
58	34857.0
59	34900.0
60	29630.5
61	24361.0
62	20476.5
63	16592.0
64	12392.0
65	8192.0
66	6984.0
67	5776.0
68	5007.0
69	4238.0
70	3151.0
71	2064.0
72	2051.5
73	2039.0
74	1230.0
75	324.0
76	227.0
77	236.0
78	245.0
79	129.5
80	14.0
81	92.5
82	171.0
83	88.5
84	6.0
85	3.5
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	849551.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.553288995994293
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.6124620517204	12.019750690527964
2	11.670942468316563	3.86384967067334
3	4.831781190259585	2.399456112233286
4	2.5091031861304023	1.661356406431464
5	1.4584607481750373	1.2071161126927725
6	0.8992493001121127	0.8931320126520842
7	0.6489243274103857	0.7519282349708736
8	0.44257310758922713	0.5860832441423801
9	0.3291846342335846	0.4904179545158283
>10	2.3303712208347505	8.11139823179962
>50	0.6504473003281019	7.90970737463919
>100	1.59299032150453	55.91267196902437
>500	0.013536143161263893	1.4803798463396363
>1k	0.009974000224089185	2.712752139357178
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	4502	0.5299269849602908	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2488	0.2928605816484237	No Hit
ATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG	1935	0.22776737358910765	No Hit
ATGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT	1689	0.19881090128785675	No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT	1669	0.1964567165479177	No Hit
ATGTCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC	1450	0.17067839364558454	No Hit
ATTGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC	1366	0.16079081773784032	No Hit
ATGCCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC	1246	0.14666570929820574	No Hit
ATTAGTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT	1165	0.1371312611014524	No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	1162	0.13677813339046155	No Hit
ATGTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT	1148	0.13513020407250417	No Hit
ATATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC	1125	0.13242289162157422	No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC	1038	0.12218218800283914	No Hit
ATGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC	1020	0.12006342173689397	No Hit
ATTTCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC	976	0.11488421530902794	No Hit
ATTGTTCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT	918	0.10805707956320455	No Hit
ATAGTTTAGGGAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATA	881	0.10370183779431724	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22211733021325383	0.0
2	0.0	0.0	0.0	1.0050014654800006	0.0
3	0.0	0.0	0.0	1.337883187707389	0.0
4	0.0	0.0	0.0	1.7543384682026153	0.0
5	0.0	0.0	0.0	3.3204598664471	0.0
6	0.0	0.0	0.0	3.651458240882537	0.0
7	0.0	0.0	0.0	4.205515619427203	0.0
8	0.0	0.0	0.0	5.836965644204998	0.0
9	0.0	0.0	0.0	6.031421303723967	0.0
10	0.0	0.0	0.0	6.421156587420884	0.0
11	0.0	0.0	0.0	6.7342631578327845	0.0
12	0.0	0.0	0.0	7.5308015645911786	0.0
13	0.0	0.0	0.0	8.281433368920759	0.0
14	0.0	0.0	0.0	8.579355447760053	0.0
15	0.0	0.0	0.0	8.740734811682877	0.0
16	0.0	0.0	0.0	8.84384810329221	0.0
17	0.0	0.0	0.0	8.986158570821528	0.0
18	2.3541847399390973E-4	0.0	0.0	9.336696678598459	0.0
19	2.3541847399390973E-4	0.0	0.0	9.798352306100517	0.0
20	3.531277109908646E-4	0.0	0.0	10.330280348089756	0.0
21	3.531277109908646E-4	0.0	0.0	10.64397546468664	0.0
22	3.531277109908646E-4	0.0	0.0	10.994395863226575	0.0
23	3.531277109908646E-4	0.0	0.0	11.496661177492582	0.0
24	3.531277109908646E-4	0.0	0.0	11.902640335895079	0.0
25	3.531277109908646E-4	0.0	0.0	12.352407330460442	0.0
26	3.531277109908646E-4	0.0	0.0	12.68069839244495	0.0
27	3.531277109908646E-4	0.0	0.0	12.968968313850493	0.0
28	3.531277109908646E-4	0.0	0.0	13.266537264978794	0.0
29	3.531277109908646E-4	0.0	0.0	13.543389390395632	0.0
30	3.531277109908646E-4	0.0	0.0	13.834013496541115	0.0
31	3.531277109908646E-4	0.0	0.0	14.102272847657174	0.0
32	3.531277109908646E-4	0.0	0.0	14.433742059040599	0.0
33	3.531277109908646E-4	0.0	0.0	14.745082990897545	0.0
34	3.531277109908646E-4	0.0	0.0	15.057718724361457	0.0
35	3.531277109908646E-4	0.0	0.0	15.368824237744409	0.0
36	3.531277109908646E-4	0.0	0.0	15.677693275624419	0.0
37	3.531277109908646E-4	0.0	0.0	16.038118959309095	0.0
38	3.531277109908646E-4	0.0	0.0	16.348165089559075	0.0
39	3.531277109908646E-4	0.0	0.0	16.67421967604064	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAT	30	2.1648302E-6	45.000004	42
GTCATCG	30	2.1648302E-6	45.000004	43
GACAGTA	30	2.1648302E-6	45.000004	11
AAACTCG	20	7.0322456E-4	45.000004	43
ACTATGT	20	7.0322456E-4	45.000004	45
ACTATCC	30	2.1648302E-6	45.000004	43
CCTTAGT	20	7.0322456E-4	45.000004	25
GATACGA	20	7.0322456E-4	45.000004	13
GGTACGT	20	7.0322456E-4	45.000004	28
CCGGGTA	20	7.0322456E-4	45.000004	35
GTACCAC	20	7.0322456E-4	45.000004	11
TACCCGG	30	2.1648302E-6	45.000004	19
CGAACGG	20	7.0322456E-4	45.000004	13
CGAACAC	20	7.0322456E-4	45.000004	17
CGTATTA	30	2.1648302E-6	45.000004	26
ACGCCCG	30	2.1648302E-6	45.000004	28
CTCCGTC	20	7.0322456E-4	45.000004	22
CTACTAT	40	6.8121153E-9	45.000004	35
ATCTAAT	30	2.1648302E-6	45.000004	1
GATCGCG	20	7.0322456E-4	45.000004	11
>>END_MODULE