FastQCFastQC Report
Thu 26 May 2016
SRR1545601_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545601_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences746635
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCTT37780.50600360283137Illumina Single End Adapter 2 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC30510.40863340186302544No Hit
TTCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC29600.3964453849605229No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT28010.37514983894406234No Hit
TGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC26320.35251495041084335No Hit
GCCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC21600.28929798361984105No Hit
ATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC18260.24456394356010633No Hit
GGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC18130.24282279828832026No Hit
AGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC12410.16621240632973272No Hit
ACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC10780.1443811233065688No Hit
TCCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC10730.14371145204818955No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT9730.13031802688060432No Hit
CTCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC9000.12054082650826708No Hit
GTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT8030.10754920409570942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAT351.0189251E-746.00000426
TCACGAT351.0189251E-746.00000440
AACGTTG351.0189251E-746.00000439
CGGGTGA351.0189251E-746.00000434
GACAGTA351.0189251E-746.0000049
CTATGCA351.0189251E-746.00000425
GATAAAC351.0189251E-746.0000049
CTATCGA351.0189251E-746.00000437
GCGCGTA351.0189251E-746.00000421
TCCGACT351.0189251E-746.00000422
GCAACCG351.0189251E-746.00000432
CGGTTAT351.0189251E-746.00000446
CGTTGGC351.0189251E-746.0000043
GAGTACG351.0189251E-746.00000416
CCGTGAT351.0189251E-746.00000416
TATATCC351.0189251E-746.00000434
ATTAACG551.8189894E-1246.00000417
GTCTACT551.8189894E-1246.00000420
CGATTAA1100.046.00000424
TGAGTAC351.0189251E-746.00000415
Produced by FastQC (version 0.11.5)