SRR1545600_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1545600_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1643402
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	4928	0.2998657662580428	Illumina PCR Primer Index 8 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4078	0.24814378952928134	No Hit
ATCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG	2823	0.17177781212387475	Illumina PCR Primer Index 8 (95% over 22bp)
ATGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT	1939	0.11798695632596284	Illumina PCR Primer Index 8 (95% over 21bp)
ATGTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTC	1866	0.11354495126572806	No Hit
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT	1859	0.1131190055750206	Illumina PCR Primer Index 8 (95% over 24bp)
ATGCCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTC	1830	0.11135437342780403	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTA	20	7.034174E-4	45.0	30
CGAACTA	20	7.034174E-4	45.0	21
CGAACGA	40	6.8175723E-9	45.0	3
ACCGGCA	50	2.1827873E-11	45.0	33
CGAGTTA	25	3.89154E-5	45.0	42
ACGTTCC	25	3.89154E-5	45.0	37
CGTTGTC	40	6.8175723E-9	45.0	21
CGCATCG	35	1.2123564E-7	45.0	18
CGCATCC	25	3.89154E-5	45.0	17
ATTACGA	50	2.1827873E-11	45.0	1
GTGATCG	25	3.89154E-5	45.0	12
ATCGTAC	100	0.0	45.0	1
GTTCGAC	25	3.89154E-5	45.0	25
CGGATGT	20	7.034174E-4	45.0	35
CGCTAAC	20	7.034174E-4	45.0	16
TCTAACG	20	7.034174E-4	45.0	2
GTCACGA	20	7.034174E-4	45.0	27
GTCGTAG	40	6.8175723E-9	45.0	3
AACGATC	20	7.034174E-4	45.0	28
GGACGTA	55	1.8189894E-12	45.0	11