FastQCFastQC Report
Thu 26 May 2016
SRR1545598_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545598_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2011354
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC78940.39247193681470294TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49300.24510851893798902No Hit
ATGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT35550.17674660949788054No Hit
ATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG34860.1733160845877951TruSeq Adapter, Index 27 (95% over 21bp)
ATGCCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC28680.14259051365398634No Hit
ATTGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC28210.14025377929494262No Hit
ATGTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC27130.1348842620443741No Hit
ATATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC25490.12673055066388114No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT24130.11996893634835043TruSeq Adapter, Index 27 (95% over 23bp)
ATGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC23120.11494744336402243No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC22380.1112683296923366No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA406.8193913E-945.00000429
CGAACGC207.0345483E-445.00000440
CGAACAG207.0345483E-445.00000443
AACGACT302.1663182E-645.0000041
TCGTATC207.0345483E-445.00000425
ACCGTAA302.1663182E-645.00000428
ACGACAC207.0345483E-445.00000430
CGTATCC253.8918537E-545.041
CAACGAA351.2125383E-745.041
CACGATC351.2125383E-745.029
CGATTAC351.2125383E-745.029
CCCGTAG253.8918537E-545.032
CTACCGT253.8918537E-545.017
CCGCGTA253.8918537E-545.019
ATCGATC700.045.01
CGGACTA351.2125383E-745.040
TCGTAGA650.044.9999962
GCGATTA1300.043.2692325
ATGCGAT1950.042.692311
GCCGTAA650.041.5384631