SRR1545598_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1545598_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2011354
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	7894	0.39247193681470294	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4930	0.24510851893798902	No Hit
ATGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT	3555	0.17674660949788054	No Hit
ATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG	3486	0.1733160845877951	TruSeq Adapter, Index 27 (95% over 21bp)
ATGCCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	2868	0.14259051365398634	No Hit
ATTGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	2821	0.14025377929494262	No Hit
ATGTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	2713	0.1348842620443741	No Hit
ATATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	2549	0.12673055066388114	No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	2413	0.11996893634835043	TruSeq Adapter, Index 27 (95% over 23bp)
ATGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	2312	0.11494744336402243	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	2238	0.1112683296923366	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	40	6.8193913E-9	45.000004	29
CGAACGC	20	7.0345483E-4	45.000004	40
CGAACAG	20	7.0345483E-4	45.000004	43
AACGACT	30	2.1663182E-6	45.000004	1
TCGTATC	20	7.0345483E-4	45.000004	25
ACCGTAA	30	2.1663182E-6	45.000004	28
ACGACAC	20	7.0345483E-4	45.000004	30
CGTATCC	25	3.8918537E-5	45.0	41
CAACGAA	35	1.2125383E-7	45.0	41
CACGATC	35	1.2125383E-7	45.0	29
CGATTAC	35	1.2125383E-7	45.0	29
CCCGTAG	25	3.8918537E-5	45.0	32
CTACCGT	25	3.8918537E-5	45.0	17
CCGCGTA	25	3.8918537E-5	45.0	19
ATCGATC	70	0.0	45.0	1
CGGACTA	35	1.2125383E-7	45.0	40
TCGTAGA	65	0.0	44.999996	2
GCGATTA	130	0.0	43.26923	25
ATGCGAT	195	0.0	42.69231	1
GCCGTAA	65	0.0	41.53846	31