##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545598_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2011354
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87008353576745	31.0	31.0	34.0	31.0	34.0
2	32.55101638001068	34.0	31.0	34.0	31.0	34.0
3	32.52572645093802	34.0	31.0	34.0	31.0	34.0
4	36.00863448204543	37.0	35.0	37.0	35.0	37.0
5	35.781973237928284	37.0	35.0	37.0	35.0	37.0
6	35.7876246548345	37.0	35.0	37.0	35.0	37.0
7	35.76808508099519	37.0	35.0	37.0	35.0	37.0
8	35.73016982589837	37.0	35.0	37.0	33.0	37.0
9	37.89159342413121	39.0	38.0	39.0	35.0	39.0
10	37.831766064054364	39.0	38.0	39.0	35.0	39.0
11	37.67863836997366	39.0	38.0	39.0	35.0	39.0
12	37.21368540793913	39.0	37.0	39.0	33.0	39.0
13	37.003742255217134	39.0	37.0	39.0	33.0	39.0
14	37.684469765143284	40.0	36.0	41.0	33.0	41.0
15	37.55189837293684	40.0	36.0	41.0	32.0	41.0
16	37.41928273193083	40.0	36.0	41.0	32.0	41.0
17	37.56998221098822	40.0	36.0	41.0	32.0	41.0
18	37.655827865209204	40.0	35.0	41.0	33.0	41.0
19	37.67245397876256	40.0	35.0	41.0	33.0	41.0
20	37.64006286312603	40.0	35.0	41.0	33.0	41.0
21	37.62563526858027	40.0	35.0	41.0	33.0	41.0
22	37.524375122429966	40.0	35.0	41.0	32.0	41.0
23	37.456715227652616	39.0	35.0	41.0	32.0	41.0
24	37.45317681522	39.0	35.0	41.0	32.0	41.0
25	37.41736263233623	39.0	35.0	41.0	32.0	41.0
26	37.315578958253994	39.0	35.0	41.0	32.0	41.0
27	37.22088851589526	39.0	35.0	41.0	32.0	41.0
28	37.147485226369895	39.0	35.0	41.0	32.0	41.0
29	37.06385002341706	39.0	35.0	41.0	31.0	41.0
30	36.96315119069045	39.0	35.0	41.0	31.0	41.0
31	36.84443563887809	39.0	35.0	41.0	31.0	41.0
32	36.74768389850817	39.0	35.0	41.0	31.0	41.0
33	36.569578502839384	39.0	35.0	41.0	30.0	41.0
34	36.536997465388986	39.0	35.0	41.0	30.0	41.0
35	36.47746393722836	39.0	35.0	41.0	30.0	41.0
36	36.33348033215436	38.0	35.0	41.0	30.0	41.0
37	36.24705695765141	38.0	35.0	41.0	30.0	41.0
38	36.14374048526515	38.0	35.0	41.0	30.0	41.0
39	36.0619264435798	38.0	35.0	40.0	30.0	41.0
40	35.96459399986278	38.0	35.0	40.0	29.0	41.0
41	35.886254234709554	38.0	35.0	40.0	29.0	41.0
42	35.7473373657745	38.0	35.0	40.0	28.0	41.0
43	35.61369853342574	38.0	34.0	40.0	28.0	41.0
44	35.54872140856359	38.0	34.0	40.0	28.0	41.0
45	35.51865658655811	37.0	34.0	40.0	28.0	41.0
46	35.44819907385771	37.0	34.0	40.0	28.0	41.0
47	35.35391333400287	37.0	34.0	40.0	28.0	41.0
48	35.29803754088042	37.0	34.0	40.0	28.0	41.0
49	35.25348745173649	37.0	34.0	40.0	28.0	41.0
50	35.12859794944102	36.0	34.0	40.0	28.0	41.0
51	34.96821693247434	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	24.0
11	22.0
12	28.0
13	34.0
14	47.0
15	66.0
16	140.0
17	257.0
18	592.0
19	1125.0
20	1883.0
21	3069.0
22	4824.0
23	7173.0
24	10367.0
25	14717.0
26	19811.0
27	24079.0
28	28543.0
29	33732.0
30	40976.0
31	50357.0
32	63890.0
33	82372.0
34	134617.0
35	220225.0
36	149395.0
37	209834.0
38	314714.0
39	594187.0
40	243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.0983377366689306	91.13905359275394	3.3287029533339236	3.4339057172432104
2	3.4687081438672656	2.629522202456654	90.90001063959899	3.0017590140770842
3	28.57144987903671	16.16721869944326	41.65383120027603	13.607500221243999
4	28.051451907521002	15.14646352655972	43.263940609161786	13.538143956757487
5	25.229273414824043	13.644539946722457	46.069911114602405	15.056275523851099
6	23.427253481982785	14.002010585903824	46.99799239716131	15.572743534952076
7	23.71049551695027	16.868437878165654	43.80148894724648	15.619577657637592
8	25.97767474049819	19.06551507094226	43.24484899227088	11.71196119628867
9	81.61795487020186	4.415433583546209	9.300451337755563	4.6661602084963665
10	82.10523856069096	4.572790269639258	9.190425951871227	4.131545217798557
11	76.16928695793978	6.84245538080318	10.425166330740387	6.563091330516658
12	43.03334967390126	21.834246979895138	17.3511475354413	17.781255810762303
13	35.36801577444846	23.21103097714276	23.761356777573713	17.65959647083507
14	30.363476543661633	22.937931363648566	26.75555869329815	19.943033399391656
15	23.35038983689594	25.112188108110256	32.03588229620445	19.501539758789352
16	19.22252373276907	29.948383029541294	32.065663229844176	18.76343000784546
17	19.031508128355327	22.644447471703142	38.259997991402805	20.064046408538726
18	23.653170948525222	21.43799649390411	34.35397249812813	20.554860059442547
19	23.567358108020766	21.14908663517213	28.475096875040396	26.80845838176671
20	24.145774438512564	22.522141800995747	30.710605890360426	22.621477870131265
21	26.51189198917744	24.467050554004917	27.363059908897192	21.657997547920456
22	26.36602010387033	24.37134388078876	26.54505372997493	22.717582285365978
23	25.27217983507627	23.541256288052725	29.7748183561919	21.411745520679105
24	27.888974292938983	20.74612425261789	27.14479897621204	24.22010247823108
25	26.77037458348953	24.196387110374403	25.319908877303547	23.71332942883252
26	25.922637188679865	22.130316194961207	29.437831430966405	22.509215185392524
27	28.614455734793577	22.61874339375366	26.07820403568939	22.688596835763374
28	23.731874150447908	26.66253677870728	24.624954135373482	24.98063493547133
29	24.038085786987274	24.941457346643105	27.723613048722402	23.29684381764722
30	23.880132487866383	24.551421579692086	29.003646299955154	22.564799632486373
31	23.498499021057455	25.445247330902465	28.137165312520818	22.91908833551926
32	26.703852230885268	24.38775073905439	27.436343875816988	21.472053154243362
33	26.40340785361503	24.85300946526569	27.132071231618106	21.61151144950118
34	27.27520864054761	24.781863361695653	25.92646545560851	22.016462542148226
35	27.55218623872277	25.09414056401807	24.767246342513552	22.586426854745607
36	23.411045494726437	24.25749022797578	28.321767326885272	24.009696950412508
37	24.322769636772044	24.790066790828465	28.744716245872183	22.142447326527304
38	26.751879579626458	27.332384055715703	23.491041358209444	22.424695006448392
39	25.116811859076027	26.543413044148366	25.563923605690498	22.77585149108511
40	24.875283018305083	24.73458177923926	27.62735948023073	22.76277572222493
41	22.88388816687664	26.381184018327954	25.342779043370783	25.392148771424623
42	26.037137172273006	22.9095425270738	27.8090281472083	23.244292153444892
43	23.04736013650506	23.44301400946825	27.64520815331364	25.864417700713048
44	22.652750336340592	24.784597838073257	26.854745609176707	25.707906216409445
45	22.828502590792073	23.224753076783102	27.539160187614907	26.407584144809913
46	24.932160126959253	23.81674235365828	27.28231827912938	23.96877924025308
47	22.193010280636823	25.02209954090627	26.703255617857423	26.081634560599475
48	22.68695614993681	25.458621406276567	26.244460199447733	25.60996224433889
49	23.16076632954716	24.86310216898666	29.325717899484626	22.65041360198155
50	22.867729897372616	25.173440378968596	28.671581432209347	23.28724829144944
51	22.941312170806334	24.56056964611898	27.088269891824112	25.40984829125057
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	597.0
1	1186.0
2	1775.0
3	4928.5
4	8082.0
5	5757.5
6	3433.0
7	3323.5
8	3214.0
9	3425.5
10	3637.0
11	3857.0
12	4077.0
13	4186.0
14	4295.0
15	4184.0
16	4073.0
17	4719.5
18	5366.0
19	5240.0
20	5114.0
21	6298.5
22	7483.0
23	8126.0
24	8769.0
25	10502.5
26	17170.5
27	22105.0
28	22266.5
29	22428.0
30	27039.5
31	31651.0
32	36598.5
33	41546.0
34	49130.5
35	56715.0
36	62283.0
37	67851.0
38	75583.5
39	83316.0
40	98318.5
41	113321.0
42	125473.5
43	137626.0
44	152793.0
45	167960.0
46	178249.0
47	188538.0
48	182441.0
49	176344.0
50	159773.5
51	143203.0
52	132827.0
53	122451.0
54	120666.0
55	118881.0
56	115429.5
57	111978.0
58	102342.0
59	92706.0
60	83531.0
61	74356.0
62	65780.5
63	57205.0
64	49410.0
65	41615.0
66	33002.0
67	24389.0
68	20991.5
69	17594.0
70	14690.5
71	11787.0
72	8888.0
73	5989.0
74	4798.5
75	2817.0
76	2026.0
77	1606.5
78	1187.0
79	840.5
80	494.0
81	322.5
82	151.0
83	95.5
84	40.0
85	38.5
86	37.0
87	58.5
88	80.0
89	47.5
90	15.0
91	10.0
92	5.0
93	3.0
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2011354.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.549754127151765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.56601693092533	12.00949738193546
2	11.053748039239085	3.658736244657855
3	4.535839571818514	2.252010890214052
4	2.335461515822603	1.5460525544115695
5	1.4535402694427972	1.202786703659611
6	0.9572431765931326	0.9505283527506035
7	0.6167389888412619	0.7144815038165756
8	0.46363974851926276	0.613849907325462
9	0.373129364699921	0.555767931906363
>10	2.8990671073857524	10.428446657893279
>50	1.0110365819438474	12.39665558556167
>100	1.7191514245559185	50.12503070609268
>500	0.009051341301519154	0.9889629171492227
>1k	0.006034227534346103	2.1630255785109145
>5k	3.017113767173051E-4	0.3941670841147109
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	7894	0.39247193681470294	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4930	0.24510851893798902	No Hit
ATGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT	3555	0.17674660949788054	No Hit
ATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG	3486	0.1733160845877951	TruSeq Adapter, Index 27 (95% over 21bp)
ATGCCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	2868	0.14259051365398634	No Hit
ATTGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	2821	0.14025377929494262	No Hit
ATGTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	2713	0.1348842620443741	No Hit
ATATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	2549	0.12673055066388114	No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	2413	0.11996893634835043	TruSeq Adapter, Index 27 (95% over 23bp)
ATGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	2312	0.11494744336402243	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	2238	0.1112683296923366	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13145373713428865	0.0
2	0.0	0.0	0.0	0.7065389782206414	0.0
3	0.0	0.0	0.0	0.9654690323036124	0.0
4	0.0	0.0	0.0	1.3106096689096003	0.0
5	0.0	0.0	0.0	2.6337979291561804	0.0
6	0.0	0.0	0.0	2.922906658897439	0.0
7	0.0	0.0	0.0	3.4032795818140418	0.0
8	0.0	0.0	0.0	4.811932658298837	0.0
9	0.0	0.0	0.0	4.987734630502636	0.0
10	0.0	0.0	0.0	5.3298424842170995	0.0
11	4.971775232007891E-5	0.0	0.0	5.5873804412351085	0.0
12	4.971775232007891E-5	0.0	0.0	6.289792846013183	0.0
13	4.971775232007891E-5	0.0	0.0	6.96232488164689	0.0
14	9.943550464015783E-5	0.0	0.0	7.243478771016937	0.0
15	1.4915325696023674E-4	0.0	0.0	7.39631114164886	0.0
16	1.4915325696023674E-4	0.0	0.0	7.510661971985041	0.0
17	1.4915325696023674E-4	0.0	0.0	7.64882760568254	0.0
18	1.4915325696023674E-4	0.0	0.0	7.964087873144161	0.0
19	1.4915325696023674E-4	0.0	0.0	8.33562863623211	0.0
20	1.4915325696023674E-4	0.0	0.0	8.74445771356012	0.0
21	1.4915325696023674E-4	0.0	0.0	9.011442043518944	0.0
22	1.4915325696023674E-4	0.0	0.0	9.327696666026965	0.0
23	1.4915325696023674E-4	0.0	0.0	9.769289742133905	0.0
24	1.4915325696023674E-4	0.0	0.0	10.161214783673088	0.0
25	1.4915325696023674E-4	0.0	0.0	10.574717329719185	0.0
26	2.983065139204735E-4	0.0	0.0	10.88684537878464	0.0
27	3.480242662405524E-4	0.0	0.0	11.171578946321732	0.0
28	3.480242662405524E-4	0.0	0.0	11.448457108992251	0.0
29	3.480242662405524E-4	0.0	0.0	11.776544556552452	0.0
30	3.480242662405524E-4	0.0	0.0	12.045020419080878	0.0
31	3.480242662405524E-4	0.0	0.0	12.376389238294204	0.0
32	3.480242662405524E-4	0.0	0.0	12.740024878763261	0.0
33	3.480242662405524E-4	0.0	0.0	13.05369417815064	0.0
34	3.977420185606313E-4	0.0	0.0	13.38894098204493	0.0
35	4.474597708807102E-4	0.0	0.0	13.714095082218247	0.0
36	4.474597708807102E-4	0.0	0.0	14.069925035573052	0.0
37	4.474597708807102E-4	0.0	0.0	14.461253464084393	0.0
38	4.474597708807102E-4	0.0	0.0	14.783772523384744	0.0
39	4.474597708807102E-4	0.0	0.0	15.128614853476812	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	40	6.8193913E-9	45.000004	29
CGAACGC	20	7.0345483E-4	45.000004	40
CGAACAG	20	7.0345483E-4	45.000004	43
AACGACT	30	2.1663182E-6	45.000004	1
TCGTATC	20	7.0345483E-4	45.000004	25
ACCGTAA	30	2.1663182E-6	45.000004	28
ACGACAC	20	7.0345483E-4	45.000004	30
CGTATCC	25	3.8918537E-5	45.0	41
CAACGAA	35	1.2125383E-7	45.0	41
CACGATC	35	1.2125383E-7	45.0	29
CGATTAC	35	1.2125383E-7	45.0	29
CCCGTAG	25	3.8918537E-5	45.0	32
CTACCGT	25	3.8918537E-5	45.0	17
CCGCGTA	25	3.8918537E-5	45.0	19
ATCGATC	70	0.0	45.0	1
CGGACTA	35	1.2125383E-7	45.0	40
TCGTAGA	65	0.0	44.999996	2
GCGATTA	130	0.0	43.26923	25
ATGCGAT	195	0.0	42.69231	1
GCCGTAA	65	0.0	41.53846	31
>>END_MODULE