FastQCFastQC Report
Thu 26 May 2016
SRR1545597_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545597_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1661717
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCTT62750.37762146021253923TruSeq Adapter, Index 27 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT54140.3258075833610657Illumina Single End Adapter 1 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC53810.3238216856420197No Hit
TGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC51270.3085362910772412TruSeq Adapter, Index 27 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC44690.2689386941338387No Hit
TTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC42600.25636134191321386TruSeq Adapter, Index 27 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC39760.23927058578566626No Hit
ATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC37710.22693394844007733TruSeq Adapter, Index 27 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC26670.16049664293017404TruSeq Adapter, Index 27 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC18700.11253420407927463TruSeq Adapter, Index 27 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC17460.10507204295316229TruSeq Adapter, Index 27 (95% over 22bp)
CTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC16790.10104006879631128TruSeq Adapter, Index 27 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAA206.3122704E-446.011
CGTATCC253.4178178E-546.039
CGTATAG206.3122704E-446.01
CAAGTCG253.4178178E-546.025
CCGATTA206.3122704E-446.022
CAACGAA453.110472E-1046.039
ACGCATC405.6134013E-946.036
CGGCATA301.8619085E-646.029
ATTACGT206.3122704E-446.016
CGCGATC253.4178178E-546.036
CGATTAC206.3122704E-446.027
TAACGAC206.3122704E-446.011
CCGTCTA253.4178178E-546.045
CATTACG206.3122704E-446.019
CCGTAAG253.4178178E-546.01
TACGAAC206.3122704E-446.044
TCGCTAC206.3122704E-446.037
AACGCAT206.3122704E-446.035
ACTACGT301.8619085E-646.046
ACTACGG206.3122704E-446.018
Produced by FastQC (version 0.11.5)