##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545597_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1661717
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.558580071095136	33.0	31.0	34.0	31.0	34.0
2	32.96180456720368	34.0	31.0	34.0	31.0	34.0
3	33.03306700238368	34.0	33.0	34.0	31.0	34.0
4	36.416174956385476	37.0	37.0	37.0	35.0	37.0
5	36.39305609799984	37.0	37.0	37.0	35.0	37.0
6	36.39358747608648	37.0	37.0	37.0	35.0	37.0
7	36.46626050043419	37.0	37.0	37.0	35.0	37.0
8	36.51104249399868	37.0	37.0	37.0	35.0	37.0
9	38.323579767192605	39.0	39.0	39.0	37.0	39.0
10	38.08446263713978	39.0	39.0	39.0	35.0	39.0
11	37.93781131203448	39.0	38.0	39.0	35.0	39.0
12	37.526340526094394	39.0	37.0	39.0	35.0	39.0
13	37.404390759678094	39.0	37.0	39.0	35.0	39.0
14	38.502282879696125	41.0	38.0	41.0	34.0	41.0
15	38.63750145181159	41.0	38.0	41.0	35.0	41.0
16	38.76337306532942	41.0	38.0	41.0	35.0	41.0
17	38.76980917930069	40.0	38.0	41.0	35.0	41.0
18	38.77232464974481	40.0	38.0	41.0	35.0	41.0
19	38.744593092566305	40.0	38.0	41.0	35.0	41.0
20	38.61168417967681	40.0	38.0	41.0	35.0	41.0
21	38.569287068736735	40.0	37.0	41.0	35.0	41.0
22	38.71790804330701	40.0	38.0	41.0	35.0	41.0
23	38.74320838024766	40.0	38.0	41.0	35.0	41.0
24	38.69724688379549	40.0	37.0	41.0	35.0	41.0
25	38.62728190179194	40.0	37.0	41.0	35.0	41.0
26	38.536575120793735	40.0	37.0	41.0	35.0	41.0
27	38.43108904825551	40.0	37.0	41.0	35.0	41.0
28	38.37942020211624	40.0	36.0	41.0	35.0	41.0
29	38.272558444067194	40.0	36.0	41.0	34.0	41.0
30	38.22332081816579	40.0	36.0	41.0	34.0	41.0
31	38.06801458972858	40.0	36.0	41.0	34.0	41.0
32	38.033665178848146	40.0	35.0	41.0	34.0	41.0
33	38.01919400234817	40.0	35.0	41.0	34.0	41.0
34	37.85512996496997	40.0	35.0	41.0	34.0	41.0
35	37.84698176645	40.0	35.0	41.0	34.0	41.0
36	37.737318689042716	40.0	35.0	41.0	33.0	41.0
37	37.632776820601826	40.0	35.0	41.0	33.0	41.0
38	37.543315739081926	40.0	35.0	41.0	33.0	41.0
39	37.430192385346004	40.0	35.0	41.0	33.0	41.0
40	37.30901049938106	39.0	35.0	41.0	33.0	41.0
41	37.14239608790185	39.0	35.0	41.0	33.0	41.0
42	37.09561014300269	39.0	35.0	41.0	33.0	41.0
43	37.03281545533927	39.0	35.0	41.0	33.0	41.0
44	36.9117075891984	39.0	35.0	41.0	32.0	41.0
45	36.81310656387339	39.0	35.0	41.0	32.0	41.0
46	36.793002659297585	39.0	35.0	41.0	32.0	41.0
47	36.74368138497711	38.0	35.0	41.0	32.0	41.0
48	36.61878165776724	38.0	35.0	40.0	32.0	41.0
49	36.53990420751548	38.0	35.0	40.0	32.0	41.0
50	36.42709679205304	38.0	35.0	40.0	32.0	41.0
51	36.26639373611752	37.0	35.0	40.0	32.0	41.0
52	35.86736790921679	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	6.0
11	4.0
12	15.0
13	14.0
14	18.0
15	50.0
16	81.0
17	179.0
18	297.0
19	516.0
20	812.0
21	1371.0
22	2047.0
23	2823.0
24	4028.0
25	5223.0
26	6500.0
27	8299.0
28	10779.0
29	13634.0
30	17187.0
31	22685.0
32	30253.0
33	42560.0
34	104097.0
35	168070.0
36	98185.0
37	146965.0
38	256684.0
39	717547.0
40	782.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.24169217742853	13.78568071458618	45.47669669384137	12.495930414143924
2	26.43452525309665	12.435089729478605	49.10709826041378	12.023286757010972
3	26.085127611982063	13.101629218452961	45.43691856074169	15.376324608823284
4	23.627669452740747	14.039755265186551	47.15664580671678	15.175929475355913
5	24.306003970591863	16.282676292052138	43.75877480942904	15.652544927926956
6	25.174563418440083	18.65022744546755	44.35634948670562	11.818859649386749
7	85.32162817134325	2.8771445438663745	6.937523055971624	4.8637042288187455
8	86.26276315401479	3.061893210456413	7.25394275920629	3.421400876322503
9	79.48056137116008	4.610472180280999	9.17111638142957	6.7378500671293615
10	44.344734993985135	20.613317430103923	15.724759390437724	19.31718818547322
11	36.098445162443426	21.621912756504265	24.129981218221875	18.149660862830434
12	31.346372456922566	22.733654406857482	25.458065362513594	20.461907773706354
13	24.71931141102847	23.509418270379374	32.88923444846505	18.882035870127105
14	19.154946359699036	30.290356300140157	31.95405715895065	18.600640181210156
15	19.030857841618037	22.233689611407957	37.64076554551708	21.094687001456926
16	23.771556769293447	22.117665041640663	33.19831234801112	20.912465841054765
17	24.09688292290444	21.47898829945171	26.893267626196277	27.530861151447567
18	24.3622710726315	22.626115036435205	29.069029202926856	23.94258468800644
19	26.135497199583323	25.22848355044812	26.88724975432038	21.748769495648176
20	26.375610287431616	23.985251399606554	25.24659734479457	24.392540968167264
21	24.78093441903766	24.024307388081123	29.913396805833965	21.281361387047255
22	27.722710906851166	20.12496712737488	27.07476664197333	25.07755532380062
23	26.693714994791534	24.40048455904345	24.331038317595596	24.574762128569425
24	25.7428912383998	21.44877858263471	28.512075160812582	24.29625501815291
25	30.584991307184072	22.23194442856395	23.77480642010643	23.408257844145545
26	23.84148444049137	25.852897936291196	23.56213482801223	26.743482795205196
27	24.796761422071267	23.330446760790196	26.34949272349022	25.523299093648316
28	26.078628310356095	23.422038770741345	26.817984049028805	23.68134886987375
29	24.333325108908436	25.422319203570765	26.083201892981776	24.161153794539022
30	29.496538820990576	23.055851267093015	26.115277150080306	21.3323327618361
31	27.18778227580268	24.45229843589492	26.607779784403725	21.75213950389868
32	27.98888137992209	25.296545681364517	24.58685805103998	22.127714887673413
33	28.902093437089466	24.908934553837987	23.748929571040076	22.440042438032467
34	23.01456866602436	23.425408778991848	28.489086890246657	25.07093566473714
35	24.763001160847484	24.020997558549382	28.734014275595666	22.48198700500747
36	27.098477057164366	26.629985731625784	22.809660128650062	23.461877082559788
37	26.34209074108287	25.83977897560174	24.089059689465774	23.729070593849613
38	24.752409706345908	23.438708275837584	28.987607396445963	22.821274621370545
39	23.138416469230318	25.30840088896003	25.501273682582532	26.05190895922711
40	26.83483409028132	21.869668541634947	28.545353992286294	22.750143375797443
41	23.71312323337849	22.28442027132177	27.12044228951139	26.88201420578835
42	22.717586688948842	24.520841996561387	25.883228010545718	26.878343303944053
43	23.092680642973505	21.72469800814459	27.6984588831913	27.48416246569061
44	27.392630634458214	22.283818484134184	25.465166451327153	24.858384430080452
45	22.666013526972403	22.904983219164272	26.75028299042496	27.67872026343836
46	22.9863448469264	23.960277231321577	25.78784474131275	27.265533180439267
47	23.658300420589065	23.665943117871453	29.801283852786003	22.874472608753475
48	22.634961308092773	23.703554817095814	30.31575171945644	23.345732155354973
49	23.359693618107055	22.223459229218932	26.79680114002565	27.620046012648363
50	21.55108240452496	23.973095298417242	29.364807605627192	25.11101469143061
51	23.393935309080906	24.435207679767373	26.68300318285243	25.487853828299283
52	22.878564761629086	22.527241401514218	27.685339922501846	26.908853914354854
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	209.0
1	167.0
2	125.0
3	113.0
4	101.0
5	149.0
6	197.0
7	256.5
8	316.0
9	402.0
10	488.0
11	762.5
12	1037.0
13	1039.5
14	1032.0
15	1022.0
16	1033.5
17	1045.0
18	1410.5
19	1776.0
20	1734.5
21	1693.0
22	2856.0
23	4019.0
24	4837.5
25	5656.0
26	7330.5
27	9005.0
28	10316.5
29	11628.0
30	12669.5
31	13711.0
32	18988.5
33	24266.0
34	25972.5
35	27679.0
36	32319.5
37	36960.0
38	40526.0
39	54148.5
40	64205.0
41	71315.0
42	78425.0
43	89715.0
44	101005.0
45	112363.0
46	123721.0
47	142095.5
48	160470.0
49	167608.5
50	174747.0
51	158454.0
52	142161.0
53	127222.0
54	112283.0
55	107460.5
56	102638.0
57	98102.0
58	93566.0
59	87746.0
60	81926.0
61	74842.0
62	67758.0
63	58825.5
64	45710.0
65	41527.0
66	35091.0
67	28655.0
68	23848.0
69	19041.0
70	16023.5
71	13006.0
72	10724.5
73	8443.0
74	6759.0
75	5075.0
76	4467.5
77	3860.0
78	2687.5
79	1515.0
80	1140.0
81	765.0
82	626.5
83	488.0
84	402.5
85	317.0
86	202.5
87	88.0
88	66.0
89	26.0
90	8.0
91	8.0
92	8.0
93	6.5
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	2.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1661717.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.262229841208892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.52594455806391	11.829106050472369
2	12.55072424272817	4.333069731032121
3	5.330535349072497	2.7605077911713436
4	2.7536183574139304	1.9013437192260543
5	1.6789666401795431	1.449135401925077
6	1.0851220797270709	1.1238976047611577
7	0.7824309148481711	0.9454551600883531
8	0.5693413321097069	0.7862480746382244
9	0.43332941740697806	0.673220880021276
>10	3.4660102187390254	12.827668338422221
>50	1.2437368276905996	15.842362614841884
>100	1.568371680728433	41.586978255378796
>500	0.005934190645976938	0.7469491470108364
>1k	0.004537910493982365	1.8565265585191892
>5k	0.0013962801519945738	1.3375306724910887
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCTT	6275	0.37762146021253923	TruSeq Adapter, Index 27 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	5414	0.3258075833610657	Illumina Single End Adapter 1 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	5381	0.3238216856420197	No Hit
TGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	5127	0.3085362910772412	TruSeq Adapter, Index 27 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	4469	0.2689386941338387	No Hit
TTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	4260	0.25636134191321386	TruSeq Adapter, Index 27 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	3976	0.23927058578566626	No Hit
ATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	3771	0.22693394844007733	TruSeq Adapter, Index 27 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	2667	0.16049664293017404	TruSeq Adapter, Index 27 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	1870	0.11253420407927463	TruSeq Adapter, Index 27 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	1746	0.10507204295316229	TruSeq Adapter, Index 27 (95% over 22bp)
CTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	1679	0.10104006879631128	TruSeq Adapter, Index 27 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.39988758615335823	0.0
2	0.0	0.0	0.0	0.9444448122032814	0.0
3	0.0	0.0	0.0	3.4151422895715697	0.0
4	0.0	0.0	0.0	3.9255180033663977	0.0
5	0.0	0.0	0.0	4.773375971961531	0.0
6	0.0	0.0	0.0	7.344752445813577	0.0
7	0.0	0.0	0.0	7.67832308389455	0.0
8	0.0	0.0	0.0	8.298705495580776	0.0
9	0.0	0.0	0.0	8.689325559045253	0.0
10	0.0	0.0	0.0	9.976006744830798	0.0
11	6.017871875897039E-5	0.0	0.0	11.148528901130577	0.0
12	6.017871875897039E-5	0.0	0.0	11.625806319607971	0.0
13	6.017871875897039E-5	0.0	0.0	11.866942445675166	0.0
14	6.017871875897039E-5	0.0	0.0	12.03616500282539	0.0
15	6.017871875897039E-5	0.0	0.0	12.241675327387275	0.0
16	6.017871875897039E-5	0.0	0.0	12.739232974086441	0.0
17	6.017871875897039E-5	0.0	0.0	13.360397709116535	0.0
18	6.017871875897039E-5	0.0	0.0	14.035061325123351	0.0
19	6.017871875897039E-5	0.0	0.0	14.457696466967601	0.0
20	6.017871875897039E-5	0.0	0.0	14.963679134293024	0.0
21	6.017871875897039E-5	0.0	0.0	15.680708568306155	0.0
22	6.017871875897039E-5	0.0	0.0	16.304942417992955	0.0
23	6.017871875897039E-5	0.0	0.0	16.93754110958725	0.0
24	1.2035743751794078E-4	0.0	0.0	17.427094986691475	0.0
25	1.2035743751794078E-4	0.0	0.0	17.855687821692865	0.0
26	1.2035743751794078E-4	0.0	0.0	18.263518998722407	0.0
27	1.2035743751794078E-4	0.0	0.0	18.73664408560543	0.0
28	1.2035743751794078E-4	0.0	0.0	19.135628990977406	0.0
29	1.2035743751794078E-4	0.0	0.0	19.61104086917327	0.0
30	1.2035743751794078E-4	0.0	0.0	20.086091675056583	0.0
31	1.2035743751794078E-4	0.0	0.0	20.497894647524216	0.0
32	1.2035743751794078E-4	0.0	0.0	20.964039003031203	0.0
33	1.2035743751794078E-4	0.0	0.0	21.409842951597653	0.0
34	1.2035743751794078E-4	0.0	0.0	21.89536485454503	0.0
35	1.2035743751794078E-4	0.0	0.0	22.431978489718766	0.0
36	1.2035743751794078E-4	0.0	0.0	22.862737758595475	0.0
37	1.2035743751794078E-4	0.0	0.0	23.31961459141358	0.0
38	1.2035743751794078E-4	0.0	0.0	23.830652271114758	0.0
39	1.2035743751794078E-4	0.0	0.0	24.70366494415114	0.0
40	1.2035743751794078E-4	0.0	0.0	25.147362637561027	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAA	20	6.3122704E-4	46.0	11
CGTATCC	25	3.4178178E-5	46.0	39
CGTATAG	20	6.3122704E-4	46.0	1
CAAGTCG	25	3.4178178E-5	46.0	25
CCGATTA	20	6.3122704E-4	46.0	22
CAACGAA	45	3.110472E-10	46.0	39
ACGCATC	40	5.6134013E-9	46.0	36
CGGCATA	30	1.8619085E-6	46.0	29
ATTACGT	20	6.3122704E-4	46.0	16
CGCGATC	25	3.4178178E-5	46.0	36
CGATTAC	20	6.3122704E-4	46.0	27
TAACGAC	20	6.3122704E-4	46.0	11
CCGTCTA	25	3.4178178E-5	46.0	45
CATTACG	20	6.3122704E-4	46.0	19
CCGTAAG	25	3.4178178E-5	46.0	1
TACGAAC	20	6.3122704E-4	46.0	44
TCGCTAC	20	6.3122704E-4	46.0	37
AACGCAT	20	6.3122704E-4	46.0	35
ACTACGT	30	1.8619085E-6	46.0	46
ACTACGG	20	6.3122704E-4	46.0	18
>>END_MODULE