FastQCFastQC Report
Thu 26 May 2016
SRR1545596_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545596_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1937673
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC79430.409924688014954TruSeq Adapter, Index 16 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53340.27527864608734287No Hit
ATGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT33650.1736619130266046TruSeq Adapter, Index 16 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG32790.16922359964761857TruSeq Adapter, Index 16 (95% over 23bp)
ATTGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC29910.15436041065752582No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT29560.1525541203288687No Hit
ATATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC26760.13810379769961184No Hit
ATGCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC26660.13758771474856696TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT26410.13629750737095475TruSeq Adapter, Index 16 (96% over 25bp)
ATGTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC25460.13139471933602834TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC22750.11740887136271187TruSeq Adapter, Index 16 (100% over 23bp)
ATGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC21870.11286734139351687TruSeq Adapter, Index 16 (95% over 21bp)
ATAGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC21530.11111265935996424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTTA850.045.0000042
AAATCGT253.8918006E-545.038
CGAACCG253.8918006E-545.031
TCCGCGA207.0344866E-445.014
GATCGGA207.0344866E-445.035
GTTTCGA207.0344866E-445.045
CCGATCA207.0344866E-445.040
CTATCGG207.0344866E-445.013
GACCGAA207.0344866E-445.036
CGTCTAG453.8562575E-1045.03
TACGGGT453.8562575E-1045.06
TAACGGA207.0344866E-445.020
CGCGAAA207.0344866E-445.016
ATCGTAC800.045.01
CTAACGG253.8918006E-545.04
GCCGAAT351.212502E-745.033
CTAAACG207.0344866E-445.025
CGGATAG351.212502E-745.042
TATTCGA502.1827873E-1145.013
CGATGTT207.0344866E-445.042
Produced by FastQC (version 0.11.5)