##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545596_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1937673
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.893918633329772	31.0	31.0	34.0	31.0	34.0
2	32.558300084689215	34.0	31.0	34.0	31.0	34.0
3	32.50506819262073	34.0	31.0	34.0	31.0	34.0
4	35.988705524616385	37.0	35.0	37.0	35.0	37.0
5	35.74701200873419	37.0	35.0	37.0	35.0	37.0
6	35.7465743703917	37.0	35.0	37.0	35.0	37.0
7	35.73233873827008	37.0	35.0	37.0	33.0	37.0
8	35.69076774048046	37.0	35.0	37.0	33.0	37.0
9	37.91272263173404	39.0	38.0	39.0	35.0	39.0
10	37.86562128904103	39.0	38.0	39.0	35.0	39.0
11	37.665001266983644	39.0	38.0	39.0	35.0	39.0
12	37.14398456292677	39.0	37.0	39.0	33.0	39.0
13	36.8692364501131	39.0	37.0	39.0	32.0	39.0
14	37.66406509251045	40.0	36.0	41.0	33.0	41.0
15	37.60605375623235	40.0	36.0	41.0	32.0	41.0
16	37.62937244829236	40.0	36.0	41.0	32.0	41.0
17	37.68711645360182	40.0	36.0	41.0	33.0	41.0
18	37.73291984767295	40.0	36.0	41.0	33.0	41.0
19	37.68844381895191	40.0	36.0	41.0	33.0	41.0
20	37.6690045224349	40.0	36.0	41.0	33.0	41.0
21	37.62592656242823	40.0	36.0	41.0	32.0	41.0
22	37.58415532445361	40.0	35.0	41.0	32.0	41.0
23	37.52595355356657	40.0	35.0	41.0	32.0	41.0
24	37.47563340150789	40.0	35.0	41.0	32.0	41.0
25	37.40418842601409	39.0	35.0	41.0	32.0	41.0
26	37.27607134950015	39.0	35.0	41.0	32.0	41.0
27	37.20340016091466	39.0	35.0	41.0	32.0	41.0
28	37.124700607378024	39.0	35.0	41.0	31.0	41.0
29	37.060283133428605	39.0	35.0	41.0	31.0	41.0
30	36.985909903270574	39.0	35.0	41.0	31.0	41.0
31	36.89180785406	39.0	35.0	41.0	31.0	41.0
32	36.780562045298666	39.0	35.0	41.0	31.0	41.0
33	36.71057758455632	39.0	35.0	41.0	31.0	41.0
34	36.61026447702992	39.0	35.0	41.0	30.0	41.0
35	36.549031234888446	39.0	35.0	41.0	30.0	41.0
36	36.442998896098565	39.0	35.0	41.0	30.0	41.0
37	36.306840731124396	38.0	35.0	41.0	30.0	41.0
38	36.224351580478235	38.0	35.0	41.0	30.0	41.0
39	36.1663490176103	38.0	35.0	41.0	30.0	41.0
40	36.066197444047575	38.0	35.0	41.0	30.0	41.0
41	36.011385305983	38.0	35.0	40.0	30.0	41.0
42	35.85734177025742	38.0	35.0	40.0	29.0	41.0
43	35.698352611611966	38.0	35.0	40.0	29.0	41.0
44	35.59959601026592	38.0	34.0	40.0	28.0	41.0
45	35.60701831526785	38.0	34.0	40.0	28.0	41.0
46	35.47633579040426	38.0	34.0	40.0	28.0	41.0
47	35.3861668093636	37.0	34.0	40.0	28.0	41.0
48	35.330471137286835	37.0	34.0	40.0	28.0	41.0
49	35.256327047959076	37.0	34.0	40.0	27.0	41.0
50	35.15969567620543	37.0	34.0	40.0	27.0	41.0
51	34.989752656924054	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	13.0
11	13.0
12	21.0
13	19.0
14	35.0
15	60.0
16	93.0
17	195.0
18	470.0
19	885.0
20	1679.0
21	2703.0
22	4533.0
23	6915.0
24	9857.0
25	14235.0
26	19216.0
27	23804.0
28	28116.0
29	33687.0
30	40724.0
31	49372.0
32	60860.0
33	77924.0
34	121769.0
35	207900.0
36	144457.0
37	197044.0
38	299749.0
39	591008.0
40	307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.8570212827448183	91.21229433449297	3.535890730788941	3.394793651973269
2	3.518550343633833	2.7026748063269705	91.05586959203127	2.72290525800793
3	26.18610054431269	15.989952897109058	43.930890299859676	13.893056258718575
4	27.544895346118775	15.28978315742646	44.540435873338794	12.624885623115976
5	23.684182005942176	13.692919290303369	47.51049325660212	15.112405447152334
6	21.99122349333453	14.252559642416445	49.040214731794265	14.716002132454753
7	22.313465687966957	16.798396839920873	45.771706577941686	15.116430894170483
8	25.060265586608267	19.139968405401735	43.53742865798306	12.262337350006941
9	82.62678996920533	3.4510983019322663	9.464496847507293	4.457614881355109
10	83.86275702866273	3.7858813122750847	8.723969421052985	3.6273922380092
11	76.76357156238437	6.412898357978874	10.215294324687395	6.608235754949364
12	41.93297837147961	25.388236301997292	16.121915307691236	16.55687001883187
13	30.92585797500404	24.430747602923713	25.956443631097713	18.686950790974535
14	28.111864076136683	22.86366172207591	28.191753716958434	20.832720484828968
15	21.984256373495427	27.263475312913993	31.277413681255815	19.474854632334765
16	18.067496424835355	31.318803533929618	29.61918755125349	20.99451248998154
17	18.060013222045207	24.121407482067408	37.341491572623454	20.47708772326394
18	21.71320960760665	21.525613454901833	34.98603737575948	21.775139561732036
19	20.746534632004472	22.640765495519627	29.14624913491595	27.46645073755995
20	21.90431512437857	23.494882779498916	31.56858768223534	23.03221441388717
21	24.551975488124157	25.74737842762943	27.90945634273688	21.79118974150953
22	26.26991241556238	23.25640084782107	27.43863386649863	23.03505287011792
23	24.95787472912096	25.21607102952872	28.37387939038217	21.452174850968145
24	28.776217659016766	21.692566289564855	25.10062327337998	24.4305927780384
25	25.924652921313346	25.09804285862475	24.493864547836502	24.483439672225398
26	25.396390412623802	21.131377688598644	30.32782105133322	23.144410847444334
27	26.444038803244922	24.29677246883246	25.923982013476994	23.335206714445626
28	22.867532344208747	27.642538240456467	25.101913480757588	24.388015934577194
29	21.375691357623296	24.90843398241086	26.458437517579075	27.25743714238677
30	20.826114623055595	24.861573650455984	30.12185234557121	24.190459380917215
31	21.051694480957313	24.03718274445688	28.52973644159773	26.381386332988072
32	21.503421888006905	25.406918504825114	28.383426924976508	24.706232682191473
33	24.581288999743506	24.835098595067382	26.318991904206747	24.264620500982364
34	24.88221696849778	24.64868943314997	25.88832068155979	24.580772916792462
35	23.85975342588765	25.045970088864323	25.040293176402827	26.053983308845197
36	22.219538590876788	22.936326201583032	26.896230684950456	27.947904522589724
37	20.85919554021757	24.158513846247534	29.164570079678047	25.817720533856846
38	22.99319854278818	27.38356781562214	26.08830282508968	23.534930816499998
39	23.591441899639413	27.068034699353294	26.01538030410704	23.32514309690025
40	22.363474125923208	25.130814126016105	28.182877090200463	24.322834657860227
41	22.146719286484355	26.551796923423094	25.088288890850002	26.21319489924254
42	24.353077118791457	23.128515492552147	27.923442190710198	24.5949651979462
43	21.27732594715414	24.2121864731562	28.608335875041867	25.902151704647792
44	20.25790729395517	25.08065086317454	26.545552319715455	28.115889523154834
45	21.4884038741315	23.056883178947118	27.48275895881297	27.971953988108417
46	22.703314749186266	24.495309580099427	26.98654520138331	25.814830469330996
47	19.91687967990471	25.16931391416405	27.55464931389352	27.359157092037716
48	20.349047543109698	25.613403293538177	26.419834512840918	27.617714650511203
49	20.733890599703873	25.128078886375565	29.85674053361945	24.281289980301114
50	20.838913480241505	23.184716925920938	30.433979314363157	25.542390279474404
51	20.082955173550957	23.26501943310352	28.218899680183394	28.433125713162127
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	693.0
1	1078.0
2	1463.0
3	5044.5
4	8626.0
5	5805.0
6	2984.0
7	3150.5
8	3317.0
9	3455.0
10	3593.0
11	3541.5
12	3490.0
13	4107.0
14	4724.0
15	4545.5
16	4367.0
17	4490.5
18	4614.0
19	5169.0
20	5724.0
21	5530.0
22	5336.0
23	6412.0
24	7488.0
25	9935.5
26	15977.5
27	19572.0
28	21458.0
29	23344.0
30	29023.0
31	34702.0
32	40426.0
33	46150.0
34	56746.0
35	67342.0
36	71641.5
37	75941.0
38	79642.5
39	83344.0
40	96077.0
41	108810.0
42	116858.5
43	124907.0
44	136408.0
45	147909.0
46	160299.0
47	172689.0
48	181105.0
49	189521.0
50	178073.0
51	166625.0
52	145676.0
53	124727.0
54	115587.5
55	106448.0
56	103756.0
57	101064.0
58	94152.5
59	87241.0
60	74891.0
61	62541.0
62	54557.0
63	46573.0
64	38160.5
65	29748.0
66	24940.0
67	20132.0
68	16637.5
69	13143.0
70	10307.5
71	7472.0
72	5829.5
73	4187.0
74	3435.5
75	1855.0
76	1026.0
77	799.5
78	573.0
79	454.0
80	335.0
81	186.5
82	38.0
83	42.0
84	46.0
85	24.0
86	2.0
87	2.5
88	3.0
89	8.0
90	13.0
91	16.0
92	19.0
93	9.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1937673.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.812748962812833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.53768513068044	12.027451414828787
2	11.129999221593637	3.742517657379122
3	4.775230790916007	2.4085426958149507
4	2.6738317254362918	1.798178462742603
5	1.6228908945575886	1.3642628602115747
6	1.1272691612407966	1.137149605287726
7	0.7681651435963908	0.9040477404888434
8	0.5514608369894489	0.7417258092101005
9	0.4295760348028815	0.6500118630223257
>10	2.741800060997949	9.790867254702961
>50	0.9675965988049704	12.067660504953343
>100	1.6597031358974799	49.67621341548612
>500	0.008628237616920175	0.9583057421961596
>1k	0.005546724182305826	2.045200707084154
>5k	6.163026869228696E-4	0.6878642665912532
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	7943	0.409924688014954	TruSeq Adapter, Index 16 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5334	0.27527864608734287	No Hit
ATGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT	3365	0.1736619130266046	TruSeq Adapter, Index 16 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG	3279	0.16922359964761857	TruSeq Adapter, Index 16 (95% over 23bp)
ATTGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC	2991	0.15436041065752582	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2956	0.1525541203288687	No Hit
ATATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC	2676	0.13810379769961184	No Hit
ATGCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC	2666	0.13758771474856696	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	2641	0.13629750737095475	TruSeq Adapter, Index 16 (96% over 25bp)
ATGTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC	2546	0.13139471933602834	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	2275	0.11740887136271187	TruSeq Adapter, Index 16 (100% over 23bp)
ATGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC	2187	0.11286734139351687	TruSeq Adapter, Index 16 (95% over 21bp)
ATAGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC	2153	0.11111265935996424	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0321659020897747E-4	0.0	0.0	0.169481641123141	0.0
2	1.0321659020897747E-4	0.0	0.0	0.8429182839416145	0.0
3	1.0321659020897747E-4	0.0	0.0	1.130634529149139	0.0
4	1.0321659020897747E-4	0.0	0.0	1.5265217608956723	0.0
5	1.0321659020897747E-4	0.0	0.0	3.047366609329851	0.0
6	1.0321659020897747E-4	0.0	0.0	3.3851429007887295	0.0
7	1.548248853134662E-4	0.0	0.0	3.93219082889631	0.0
8	2.0643318041795494E-4	0.0	0.0	5.5544975855059135	0.0
9	2.0643318041795494E-4	0.0	0.0	5.752054139165897	0.0
10	2.0643318041795494E-4	0.0	0.0	6.149231578290042	0.0
11	2.0643318041795494E-4	0.0	0.0	6.464145395017632	0.0
12	2.0643318041795494E-4	0.0	0.0	7.278472683471359	0.0
13	2.5804147552244366E-4	0.0	0.0	8.04212062613248	0.0
14	2.5804147552244366E-4	0.0	0.0	8.355589410597144	0.0
15	3.096497706269324E-4	0.0	0.0	8.524400143883927	0.0
16	3.096497706269324E-4	0.0	0.0	8.633396863144608	0.0
17	3.096497706269324E-4	0.0	0.0	8.781099803733653	0.0
18	3.096497706269324E-4	0.0	0.0	9.123159583686204	0.0
19	3.6125806573142116E-4	0.0	0.0	9.56528784784636	0.0
20	4.644746559403986E-4	0.0	0.0	10.052057287271898	0.0
21	4.644746559403986E-4	0.0	0.0	10.370944942722534	0.0
22	5.676912461493761E-4	0.0	0.0	10.721416874777116	0.0
23	5.676912461493761E-4	0.0	0.0	11.262942715308517	0.0
24	5.676912461493761E-4	0.0	0.0	11.736293998006888	0.0
25	6.709078363583536E-4	0.0	0.0	12.262492174892255	0.0
26	7.225161314628423E-4	0.0	0.0	12.659824438901714	0.0
27	8.773410167763084E-4	0.0	0.0	13.014063776498924	0.0
28	9.289493118807972E-4	0.0	0.0	13.327687385848902	0.0
29	9.80557606985286E-4	0.0	0.0	13.637698414541566	0.0
30	0.0010321659020897747	0.0	0.0	13.973100724425638	0.0
31	0.0010321659020897747	0.0	0.0	14.323882306250848	0.0
32	0.0010321659020897747	0.0	0.0	14.710841302944305	0.0
33	0.0010321659020897747	0.0	0.0	15.066319239624024	0.0
34	0.0010321659020897747	0.0	0.0	15.417152429744338	0.0
35	0.0010837741971942633	0.0	0.0	15.75900577651647	0.0
36	0.0010837741971942633	0.0	0.0	16.11107756571929	0.0
37	0.0011353824922987522	0.0	0.0	16.531427129345353	0.0
38	0.0011869907874032409	0.0	0.0	16.909561107575943	0.0
39	0.0014966405580301733	0.0	0.0	17.282224606525457	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCTTA	85	0.0	45.000004	2
AAATCGT	25	3.8918006E-5	45.0	38
CGAACCG	25	3.8918006E-5	45.0	31
TCCGCGA	20	7.0344866E-4	45.0	14
GATCGGA	20	7.0344866E-4	45.0	35
GTTTCGA	20	7.0344866E-4	45.0	45
CCGATCA	20	7.0344866E-4	45.0	40
CTATCGG	20	7.0344866E-4	45.0	13
GACCGAA	20	7.0344866E-4	45.0	36
CGTCTAG	45	3.8562575E-10	45.0	3
TACGGGT	45	3.8562575E-10	45.0	6
TAACGGA	20	7.0344866E-4	45.0	20
CGCGAAA	20	7.0344866E-4	45.0	16
ATCGTAC	80	0.0	45.0	1
CTAACGG	25	3.8918006E-5	45.0	4
GCCGAAT	35	1.212502E-7	45.0	33
CTAAACG	20	7.0344866E-4	45.0	25
CGGATAG	35	1.212502E-7	45.0	42
TATTCGA	50	2.1827873E-11	45.0	13
CGATGTT	20	7.0344866E-4	45.0	42
>>END_MODULE