FastQCFastQC Report
Thu 26 May 2016
SRR1545595_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545595_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1641974
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCTT66400.4043912997404344TruSeq Adapter, Index 16 (96% over 26bp)
TGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC59390.3616987845118132TruSeq Adapter, Index 16 (95% over 24bp)
GTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC55910.3405047826579471TruSeq Adapter, Index 16 (100% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT55520.3381295927950138TruSeq Adapter, Index 16 (100% over 24bp)
TTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC52260.31827544163305876TruSeq Adapter, Index 16 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC44070.26839645451146TruSeq Adapter, Index 16 (100% over 23bp)
ATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC42000.25578967754666027TruSeq Adapter, Index 16 (95% over 24bp)
GGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC39050.2378234978142163TruSeq Adapter, Index 16 (100% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC32300.19671444249421732TruSeq Adapter, Index 16 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC24790.15097681205670735TruSeq Adapter, Index 16 (95% over 24bp)
TCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC20180.1229008498307525TruSeq Adapter, Index 16 (95% over 24bp)
CTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC19190.11687152171715265TruSeq Adapter, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT18420.11218204429546388TruSeq Adapter, Index 16 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA351.01992555E-746.00000441
TGACGTA351.01992555E-746.00000430
CGCTAAT351.01992555E-746.00000441
ACGGTAC351.01992555E-746.00000441
CGTATAG253.4177996E-546.01
GTCGACC206.3122483E-446.024
CTATCGC206.3122483E-446.033
CGCATTA301.8618939E-646.017
GACCGTA206.3122483E-446.033
GCACGAC206.3122483E-446.043
CGTCTAT253.4177996E-546.024
CGATTAG253.4177996E-546.042
TACGGGT501.6370905E-1146.04
AAGCGAC253.4177996E-546.028
GCGCATC253.4177996E-546.014
CCTCGAA253.4177996E-546.022
ATCCGTT206.3122483E-446.041
TTATACG405.6134013E-946.01
GTTACGC253.4177996E-546.022
CCCGTTA253.4177996E-546.030
Produced by FastQC (version 0.11.5)