##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545595_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1641974
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51738334468146	33.0	31.0	34.0	31.0	34.0
2	32.92893858246233	34.0	31.0	34.0	31.0	34.0
3	33.002657167531275	34.0	33.0	34.0	31.0	34.0
4	36.39474559280476	37.0	37.0	37.0	35.0	37.0
5	36.37226472526361	37.0	37.0	37.0	35.0	37.0
6	36.371323175641024	37.0	37.0	37.0	35.0	37.0
7	36.473358896060475	37.0	37.0	37.0	35.0	37.0
8	36.5251508245563	37.0	37.0	37.0	35.0	37.0
9	38.32350146835456	39.0	39.0	39.0	37.0	39.0
10	38.05915745316308	39.0	39.0	39.0	35.0	39.0
11	37.86349540248506	39.0	38.0	39.0	35.0	39.0
12	37.55659833834153	39.0	37.0	39.0	35.0	39.0
13	37.50554941795668	39.0	37.0	39.0	35.0	39.0
14	38.85162615242385	41.0	38.0	41.0	35.0	41.0
15	38.87027687405525	41.0	38.0	41.0	35.0	41.0
16	38.92747936325423	41.0	38.0	41.0	35.0	41.0
17	38.863019146466385	41.0	38.0	41.0	35.0	41.0
18	38.8725065074112	41.0	38.0	41.0	35.0	41.0
19	38.83793166030644	40.0	38.0	41.0	35.0	41.0
20	38.78113173533807	40.0	38.0	41.0	35.0	41.0
21	38.740672507603655	40.0	38.0	41.0	35.0	41.0
22	38.82679262887232	41.0	38.0	41.0	35.0	41.0
23	38.829421172320636	41.0	38.0	41.0	35.0	41.0
24	38.76382086439858	41.0	38.0	41.0	35.0	41.0
25	38.70269992094881	40.0	38.0	41.0	35.0	41.0
26	38.60799074772195	40.0	38.0	41.0	35.0	41.0
27	38.52311364248155	40.0	37.0	41.0	35.0	41.0
28	38.47478279193215	40.0	37.0	41.0	35.0	41.0
29	38.41233113313609	40.0	37.0	41.0	35.0	41.0
30	38.36153922047487	40.0	37.0	41.0	34.0	41.0
31	38.327584358826634	40.0	37.0	41.0	34.0	41.0
32	38.24453797684981	40.0	36.0	41.0	34.0	41.0
33	38.214239689544414	40.0	36.0	41.0	34.0	41.0
34	38.13609594305391	40.0	36.0	41.0	34.0	41.0
35	38.064841465212	40.0	36.0	41.0	34.0	41.0
36	37.97798868922407	40.0	36.0	41.0	34.0	41.0
37	37.88145731905621	40.0	35.0	41.0	34.0	41.0
38	37.76485194040831	40.0	35.0	41.0	33.0	41.0
39	37.686281877788566	40.0	35.0	41.0	33.0	41.0
40	37.541473860122025	40.0	35.0	41.0	33.0	41.0
41	37.36499603526	40.0	35.0	41.0	33.0	41.0
42	37.29323545927036	39.0	35.0	41.0	33.0	41.0
43	37.2515685388441	39.0	35.0	41.0	33.0	41.0
44	37.05707033119891	39.0	35.0	41.0	32.0	41.0
45	36.937321175609355	39.0	35.0	41.0	32.0	41.0
46	36.93683213010681	39.0	35.0	41.0	32.0	41.0
47	36.87428302762407	39.0	35.0	41.0	32.0	41.0
48	36.81049699934347	39.0	35.0	41.0	32.0	41.0
49	36.745265150361696	39.0	35.0	41.0	32.0	41.0
50	36.65118022575266	38.0	35.0	41.0	32.0	41.0
51	36.51764705165855	38.0	35.0	41.0	32.0	41.0
52	36.175741516004514	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	5.0
11	3.0
12	6.0
13	8.0
14	22.0
15	30.0
16	61.0
17	100.0
18	210.0
19	391.0
20	670.0
21	1144.0
22	1747.0
23	2553.0
24	3479.0
25	4787.0
26	6358.0
27	8253.0
28	10516.0
29	13298.0
30	17289.0
31	21895.0
32	28796.0
33	39496.0
34	85782.0
35	162628.0
36	94031.0
37	137254.0
38	241892.0
39	758030.0
40	1235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.219538190007878	13.708682354288193	47.48863258492521	12.583146870778709
2	25.593279796147804	12.675231154695506	50.47321090346132	11.258278145695364
3	24.588330874910323	13.610325133041085	46.305848935488626	15.49549505655997
4	22.25772149863518	14.023608169191473	49.353156627327834	14.365513704845508
5	22.906148331215963	16.634368144684387	45.205953322037985	15.253530202061667
6	24.254038127278505	18.608516334607003	44.718795790919955	12.418649747194536
7	86.00075275247964	2.1456490784872355	7.078004889236979	4.775593279796148
8	87.64548037910467	2.2504010416730105	6.904737833851206	3.1993807453711205
9	79.43542345981118	4.250859027000428	9.067317752899863	7.246399760288531
10	42.92108157620035	24.58144891453823	14.417341565700797	18.080127943560615
11	31.225463984204378	22.714184268447614	27.057980211623327	19.002371535724684
12	28.40538278925245	23.043361222528493	27.339409759228833	21.21184622899023
13	22.31271627930771	25.61538733256434	32.95642927354513	19.115467114582813
14	17.670620850269252	31.95556080668756	29.34973391783305	21.024084425210145
15	17.686942667788895	23.60762107073559	36.68590367447962	22.019532586995897
16	21.36215311570098	22.45815098168424	33.702117085897825	22.47757881671695
17	20.445938851650514	23.04488378013294	27.649646096710423	28.85953127150613
18	21.519037451262932	24.07248835852456	29.8209350452565	24.58753914495601
19	23.672969243118345	26.63093325472876	27.599097184242872	22.09700031791003
20	26.162594535601656	22.340183218491887	25.9797049161558	25.517517329750657
21	24.233087734641355	26.509554962502452	28.00909149596766	21.24826580688854
22	28.582486689801424	21.417026091765155	24.71927082889254	25.281216389540884
23	25.937012400927177	25.569406092910118	23.023628875974893	25.46995263018781
24	25.446566145383542	20.33150342210047	29.380367776834472	24.841562655681514
25	28.604046105480357	23.88423933631105	22.914613751496674	24.59710080671192
26	22.41600658719322	27.001767384867236	24.395087863754235	26.187138164185303
27	21.301433518435736	23.85634608099763	24.823596475949074	30.018623924617565
28	21.187363502710763	23.9660311308218	30.317776042738803	24.528829323728633
29	20.948078349596276	24.0816846064554	27.088796777537283	27.88144026641104
30	21.291080126725515	24.77682350634054	26.03719669129962	27.894899675634328
31	23.316020838332395	24.608002319159745	25.597725664352783	26.478251178155077
32	24.472921008493433	24.87621606675867	23.33155092589773	27.319311998850164
33	22.999998781953916	23.83850170587354	25.221897545271727	27.939601966900817
34	20.475659176089266	22.007595735377052	25.500951903014297	32.01579318551938
35	19.899340671654972	22.65699700482468	29.131703668876607	28.31195865464374
36	22.33031704521509	25.38560293890159	26.630141524774448	25.653938491108875
37	23.691848957413455	26.032629018486286	24.323405851736997	25.952116172363265
38	20.777369190985972	23.925774707760294	29.666730411078372	25.63012569017536
39	21.996328809104163	25.985125221227616	23.742945990618608	28.27559997904961
40	24.792840812339296	21.616785649468262	28.489671578234493	25.100701959957956
41	21.059894980066677	22.695791772585924	28.612816037281952	27.631497210065447
42	20.01006106065017	24.61713766478641	25.407040549972166	29.965760724591256
43	21.23285752393156	21.69260901816959	27.757138663584197	29.317394794314648
44	25.01294173963778	22.8186317201125	24.629927148663743	27.53849939158598
45	19.81419925041444	22.961934841842808	26.946589897282173	30.27727601046058
46	19.802566910316486	24.215852382559042	25.734146825711	30.24743388141347
47	20.765554143975486	23.593187224645458	30.084398413129566	25.5568602182495
48	19.738193174800575	21.0982025293945	32.478041674228706	26.685562621576224
49	19.73423452502902	21.121345404982055	27.39586619520163	31.748553874787298
50	19.001945219595438	21.62281497758186	31.738870408423033	27.63636939439967
51	21.35557566684978	21.616481137947375	28.87402601990044	28.15391717530241
52	20.400201221213006	19.589408845694265	28.907522287198212	31.102867645894516
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	260.0
1	197.0
2	134.0
3	121.0
4	108.0
5	159.5
6	211.0
7	262.5
8	314.0
9	425.0
10	536.0
11	598.0
12	660.0
13	753.0
14	1243.0
15	1640.0
16	1447.0
17	1254.0
18	1834.0
19	2414.0
20	2241.5
21	2069.0
22	2517.0
23	2965.0
24	4204.0
25	5443.0
26	7415.0
27	9387.0
28	10356.0
29	11325.0
30	14639.5
31	17954.0
32	21038.0
33	24122.0
34	30207.0
35	36292.0
36	40776.5
37	45261.0
38	45852.0
39	55404.0
40	64365.0
41	71800.5
42	79236.0
43	84457.0
44	89678.0
45	96961.5
46	104245.0
47	117823.5
48	131402.0
49	154575.5
50	177749.0
51	180979.5
52	184210.0
53	161592.0
54	138974.0
55	117864.5
56	96755.0
57	90126.5
58	83498.0
59	81968.0
60	80438.0
61	70145.0
62	59852.0
63	52224.0
64	39255.5
65	33915.0
66	28447.5
67	22980.0
68	19507.5
69	16035.0
70	13256.5
71	10478.0
72	8256.0
73	6034.0
74	4779.0
75	3524.0
76	2800.0
77	2076.0
78	1617.5
79	1159.0
80	835.0
81	511.0
82	405.0
83	299.0
84	239.5
85	180.0
86	111.5
87	43.0
88	57.0
89	37.5
90	4.0
91	3.0
92	2.0
93	3.0
94	4.0
95	12.0
96	20.0
97	11.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1641974.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.568061051560903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.78251046510232	11.908072820789835
2	12.26731446665314	4.310258589777173
3	5.65144804219631	2.9785495270508737
4	3.0243693131506504	2.1252921894359176
5	1.9183160989304227	1.685054717110091
6	1.252741162065969	1.3204939930186979
7	0.8950953560264238	1.10075629031486
8	0.6692001535369271	0.9405239322440534
9	0.4868708333385044	0.7698038871883662
>10	3.366085039695884	12.539712419988394
>50	1.1971454058681938	15.474493869977628
>100	1.4767903142440992	40.63119932927706
>500	0.005883626749972194	0.6544759821984933
>1k	0.004499243985272855	1.801207403999272
>5k	0.0017304784558741747	1.7601050476293185
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCTT	6640	0.4043912997404344	TruSeq Adapter, Index 16 (96% over 26bp)
TGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	5939	0.3616987845118132	TruSeq Adapter, Index 16 (95% over 24bp)
GTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	5591	0.3405047826579471	TruSeq Adapter, Index 16 (100% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	5552	0.3381295927950138	TruSeq Adapter, Index 16 (100% over 24bp)
TTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	5226	0.31827544163305876	TruSeq Adapter, Index 16 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	4407	0.26839645451146	TruSeq Adapter, Index 16 (100% over 23bp)
ATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	4200	0.25578967754666027	TruSeq Adapter, Index 16 (95% over 24bp)
GGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	3905	0.2378234978142163	TruSeq Adapter, Index 16 (100% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	3230	0.19671444249421732	TruSeq Adapter, Index 16 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	2479	0.15097681205670735	TruSeq Adapter, Index 16 (95% over 24bp)
TCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	2018	0.1229008498307525	TruSeq Adapter, Index 16 (95% over 24bp)
CTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	1919	0.11687152171715265	TruSeq Adapter, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	1842	0.11218204429546388	TruSeq Adapter, Index 16 (100% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.45938608041296636	0.0
2	0.0	0.0	0.0	1.07248957657064	0.0
3	0.0	0.0	0.0	3.9667497780111014	0.0
4	0.0	0.0	0.0	4.5734585322301085	0.0
5	0.0	0.0	0.0	5.549844272808217	0.0
6	0.0	0.0	0.0	8.556469225456675	0.0
7	0.0	0.0	0.0	8.92084771135231	0.0
8	0.0	0.0	0.0	9.644610694201004	0.0
9	6.090230417777626E-5	0.0	0.0	10.14017274329557	0.0
10	6.090230417777626E-5	0.0	0.0	11.670647647283086	0.0
11	6.090230417777626E-5	0.0	0.0	13.000936677438254	0.0
12	6.090230417777626E-5	0.0	0.0	13.547047639000374	0.0
13	1.2180460835555253E-4	0.0	0.0	13.813373415169789	0.0
14	1.2180460835555253E-4	0.0	0.0	13.960330675150765	0.0
15	1.2180460835555253E-4	0.0	0.0	14.185973712129424	0.0
16	1.2180460835555253E-4	0.0	0.0	14.737078662634122	0.0
17	1.8270691253332878E-4	0.0	0.0	15.483314595724414	0.0
18	1.8270691253332878E-4	0.0	0.0	16.308236305812393	0.0
19	1.8270691253332878E-4	0.0	0.0	16.83339687473736	0.0
20	1.8270691253332878E-4	0.0	0.0	17.411298839080278	0.0
21	1.8270691253332878E-4	0.0	0.0	18.28183637499741	0.0
22	1.8270691253332878E-4	0.0	0.0	19.03069110716735	0.0
23	1.8270691253332878E-4	0.0	0.0	19.855856426472037	0.0
24	1.8270691253332878E-4	0.0	0.0	20.478947900514868	0.0
25	1.8270691253332878E-4	0.0	0.0	21.018237804009075	0.0
26	1.8270691253332878E-4	0.0	0.0	21.498026156321597	0.0
27	1.8270691253332878E-4	0.0	0.0	21.966730289273766	0.0
28	1.8270691253332878E-4	0.0	0.0	22.443838940202465	0.0
29	1.8270691253332878E-4	0.0	0.0	22.94792731188192	0.0
30	1.8270691253332878E-4	0.0	0.0	23.48928789371817	0.0
31	1.8270691253332878E-4	0.0	0.0	23.983205580599936	0.0
32	1.8270691253332878E-4	0.0	0.0	24.457269116319747	0.0
33	1.8270691253332878E-4	0.0	0.0	24.934804083377692	0.0
34	1.8270691253332878E-4	0.0	0.0	25.405517992367724	0.0
35	1.8270691253332878E-4	0.0	0.0	25.963565805548686	0.0
36	1.8270691253332878E-4	0.0	0.0	26.45127145740432	0.0
37	2.4360921671110505E-4	0.0	0.0	26.93623650557195	0.0
38	2.4360921671110505E-4	0.0	0.0	27.42217599060643	0.0
39	3.6541382506665755E-4	0.0	0.0	27.96000423880037	0.0
40	3.6541382506665755E-4	0.0	0.0	28.478282847353245	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGAA	35	1.01992555E-7	46.000004	41
TGACGTA	35	1.01992555E-7	46.000004	30
CGCTAAT	35	1.01992555E-7	46.000004	41
ACGGTAC	35	1.01992555E-7	46.000004	41
CGTATAG	25	3.4177996E-5	46.0	1
GTCGACC	20	6.3122483E-4	46.0	24
CTATCGC	20	6.3122483E-4	46.0	33
CGCATTA	30	1.8618939E-6	46.0	17
GACCGTA	20	6.3122483E-4	46.0	33
GCACGAC	20	6.3122483E-4	46.0	43
CGTCTAT	25	3.4177996E-5	46.0	24
CGATTAG	25	3.4177996E-5	46.0	42
TACGGGT	50	1.6370905E-11	46.0	4
AAGCGAC	25	3.4177996E-5	46.0	28
GCGCATC	25	3.4177996E-5	46.0	14
CCTCGAA	25	3.4177996E-5	46.0	22
ATCCGTT	20	6.3122483E-4	46.0	41
TTATACG	40	5.6134013E-9	46.0	1
GTTACGC	25	3.4177996E-5	46.0	22
CCCGTTA	25	3.4177996E-5	46.0	30
>>END_MODULE