SRR1545594_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1545594_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1374834
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	6212	0.4518363671541437	TruSeq Adapter, Index 10 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3766	0.2739239791858508	No Hit
ATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	2912	0.21180738911024893	TruSeq Adapter, Index 10 (95% over 22bp)
ATGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT	2536	0.1844586328240355	TruSeq Adapter, Index 10 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	2252	0.16380159350147	TruSeq Adapter, Index 10 (95% over 24bp)
ATGCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	2063	0.15005447930441057	No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	2005	0.14583578817515425	RNA PCR Primer, Index 10 (95% over 23bp)
ATTGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	1968	0.1431445541789045	No Hit
ATGTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	1743	0.12677894203954804	No Hit
ATATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	1612	0.11725051897174496	No Hit
ATGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	1528	0.11114069043971855	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	55	1.8189894E-12	45.000004	40
TAACGCG	55	1.8189894E-12	45.000004	3
TCGATGT	75	0.0	45.000004	2
AGCTAGT	55	1.8189894E-12	45.000004	16
ATCGCAC	85	0.0	45.000004	1
CGCTTGT	55	1.8189894E-12	45.000004	28
CTTAACG	25	3.8912065E-5	45.0	4
AACCGTA	45	3.8562575E-10	45.0	26
AGCCGTC	20	7.03377E-4	45.0	37
AACCGGT	25	3.8912065E-5	45.0	33
CGTATGA	40	6.8175723E-9	45.0	11
CTACTCG	25	3.8912065E-5	45.0	26
GTCGAAG	20	7.03377E-4	45.0	35
GTCGAAA	20	7.03377E-4	45.0	12
GGTCGAA	20	7.03377E-4	45.0	11
TCCGATT	25	3.8912065E-5	45.0	30
CTATCGA	25	3.8912065E-5	45.0	38
TCCGACA	40	6.8175723E-9	45.0	22
TAGGTCG	35	1.2121563E-7	45.0	2
GGCGTAA	25	3.8912065E-5	45.0	9