FastQCFastQC Report
Thu 26 May 2016
SRR1545593_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545593_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1119031
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTT48390.43242769860709845TruSeq Adapter, Index 10 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT36240.3238516180516894Illumina PCR Primer Index 2 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC34460.3079449988427488TruSeq Adapter, Index 10 (95% over 23bp)
GTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC34060.3043704776722003RNA PCR Primer, Index 10 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC31700.2832808027659645Illumina PCR Primer Index 2 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC30710.27443386286885707TruSeq Adapter, Index 10 (95% over 23bp)
ATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC24020.2146499962914343TruSeq Adapter, Index 10 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC23870.21330955085247863RNA PCR Primer, Index 10 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC16150.14432129226089357TruSeq Adapter, Index 10 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC14550.13002320757869978TruSeq Adapter, Index 10 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC13070.11679747924767053TruSeq Adapter, Index 10 (95% over 23bp)
TTTCCTGGGGTTTTATCCATTCACTCATAGCAGGTGATTAGACGATCTTGAT13000.11617193804282454No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT12250.10946971084804621RNA PCR Primer, Index 10 (95% over 24bp)
CTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC11580.10348238788737757TruSeq Adapter, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTG351.01954356E-746.0000041
GGTCGAA351.01954356E-746.0000049
ACGTTAT351.01954356E-746.00000438
GACGTTA351.01954356E-746.00000420
TAAGTCG351.01954356E-746.00000429
TACGGCA351.01954356E-746.00000427
TAACGCG351.01954356E-746.0000041
TTATCCG351.01954356E-746.00000426
TGCGAAT351.01954356E-746.0000044
ACTACGC351.01954356E-746.00000445
CGGGATA551.8189894E-1246.0000046
GACGGTA351.01954356E-746.0000049
GAACGTT351.01954356E-746.00000423
TTGCGAA351.01954356E-746.0000043
CGAATCT351.01954356E-746.0000046
CGCACGG351.01954356E-746.0000041
TGTCGTA351.01954356E-746.00000421
AAAGCGT750.046.00000427
TACGTTT351.01954356E-746.00000443
TAGTATG351.01954356E-746.0000041
Produced by FastQC (version 0.11.5)