##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545593_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1119031
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.581521870260964	33.0	31.0	34.0	31.0	34.0
2	32.99185009173115	34.0	31.0	34.0	31.0	34.0
3	33.06220560467047	34.0	33.0	34.0	31.0	34.0
4	36.43634179928885	37.0	37.0	37.0	35.0	37.0
5	36.42043964823137	37.0	37.0	37.0	35.0	37.0
6	36.42309194293992	37.0	37.0	37.0	35.0	37.0
7	36.490511880367926	37.0	37.0	37.0	35.0	37.0
8	36.521275996822254	37.0	37.0	37.0	35.0	37.0
9	38.32709013423221	39.0	39.0	39.0	37.0	39.0
10	38.09445850919233	39.0	39.0	39.0	35.0	39.0
11	37.91136170490362	39.0	38.0	39.0	35.0	39.0
12	37.50991438128166	39.0	37.0	39.0	35.0	39.0
13	37.40429353610401	39.0	37.0	39.0	35.0	39.0
14	38.72184148607143	41.0	38.0	41.0	35.0	41.0
15	38.740612190368275	41.0	38.0	41.0	35.0	41.0
16	38.80742356556699	41.0	38.0	41.0	35.0	41.0
17	38.74731620482364	40.0	38.0	41.0	35.0	41.0
18	38.76813332249062	40.0	37.0	41.0	35.0	41.0
19	38.736352254763275	40.0	37.0	41.0	35.0	41.0
20	38.66424701371097	40.0	37.0	41.0	35.0	41.0
21	38.606674882107825	40.0	37.0	41.0	35.0	41.0
22	38.66984382023376	40.0	37.0	41.0	35.0	41.0
23	38.67634766150357	40.0	37.0	41.0	35.0	41.0
24	38.636042254414754	40.0	37.0	41.0	35.0	41.0
25	38.55653239275766	40.0	37.0	41.0	35.0	41.0
26	38.44359897089536	40.0	36.0	41.0	35.0	41.0
27	38.33902992857213	40.0	36.0	41.0	35.0	41.0
28	38.309655407222856	40.0	36.0	41.0	35.0	41.0
29	38.27365372362338	40.0	36.0	41.0	35.0	41.0
30	38.22619123152084	40.0	36.0	41.0	35.0	41.0
31	38.15820115796613	40.0	36.0	41.0	34.0	41.0
32	38.058303121182526	40.0	35.0	41.0	34.0	41.0
33	38.03804005429698	40.0	35.0	41.0	34.0	41.0
34	37.88821936121519	40.0	35.0	41.0	34.0	41.0
35	37.83513772183255	40.0	35.0	41.0	34.0	41.0
36	37.75701209349875	40.0	35.0	41.0	34.0	41.0
37	37.63805649709436	40.0	35.0	41.0	34.0	41.0
38	37.58840014262339	40.0	35.0	41.0	34.0	41.0
39	37.50920126430814	40.0	35.0	41.0	33.0	41.0
40	37.37542838402153	39.0	35.0	41.0	33.0	41.0
41	37.2361489538717	39.0	35.0	41.0	33.0	41.0
42	37.198917634989556	39.0	35.0	41.0	33.0	41.0
43	37.127613980309754	39.0	35.0	41.0	33.0	41.0
44	37.02638443439011	39.0	35.0	41.0	33.0	41.0
45	36.947209684092755	39.0	35.0	41.0	33.0	41.0
46	36.91903977637796	39.0	35.0	41.0	33.0	41.0
47	36.87225018788577	38.0	35.0	41.0	33.0	41.0
48	36.790417781098114	38.0	35.0	41.0	33.0	41.0
49	36.72643117125442	38.0	35.0	41.0	33.0	41.0
50	36.63571161120648	38.0	35.0	40.0	33.0	41.0
51	36.54051317613185	38.0	35.0	40.0	33.0	41.0
52	36.206576046597455	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	4.0
12	6.0
13	12.0
14	26.0
15	20.0
16	55.0
17	76.0
18	221.0
19	391.0
20	572.0
21	978.0
22	1366.0
23	1872.0
24	2440.0
25	3156.0
26	3941.0
27	4962.0
28	6326.0
29	8125.0
30	10448.0
31	13748.0
32	18393.0
33	25908.0
34	63050.0
35	137440.0
36	68972.0
37	95143.0
38	160393.0
39	489993.0
40	993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.166897074343787	13.524290211799316	47.390554863985	11.918257849871898
2	25.35068286758812	12.693571491763855	49.54474004741603	12.411005593232002
3	26.123851796777746	13.828928778559307	45.73760691169414	14.309612512968808
4	22.717511847303605	13.927496199837181	47.24560803051926	16.109383922339955
5	23.77780418951754	16.73349531871771	44.19234140966604	15.296359082098709
6	25.037197360931017	19.063725669798245	43.79440784035474	12.104669128915999
7	85.58377739311959	2.9740016138963083	6.677473635672292	4.764747357311817
8	86.70251315647198	2.955324740780193	6.754147114780555	3.5880149879672683
9	79.48412510466645	4.710057183402426	9.04586200024843	6.7599557116826965
10	46.04090503301517	21.540332662812737	13.645377116451643	18.77338518772045
11	35.13244941382321	22.681230457422537	23.79522998022396	18.39109014853029
12	31.46445451466492	22.694813637870624	25.57989903764954	20.26083280981492
13	22.590080167573554	26.78102751398308	30.935693470511538	19.693198847931825
14	18.03274440118281	33.262349300421526	29.049418648813123	19.65548764958254
15	18.33595315947458	23.218927804502286	37.45704989406013	20.988069141963
16	21.336316867003685	21.463659183704472	34.92655699439962	22.273466954892225
17	20.925515021478404	22.20331697692021	27.671798189683756	29.19936981191763
18	22.97550291278794	22.129771203836178	29.744394927397007	25.150330955978877
19	25.61653787964766	25.976224072434096	26.060850861146832	22.346387186771413
20	28.965953579480818	21.8428265168704	24.35785961246829	24.833360291180494
21	26.05334436668868	25.611712276067415	26.711592440245173	21.623350916998724
22	28.140507278171917	20.574675768589074	24.469027220872345	26.815789732366664
23	27.61424839883792	25.04318468389169	21.673751665503456	25.66881525176693
24	26.334659182810842	20.15824405222018	28.65130635344329	24.855790411525685
25	28.200916685954187	22.140316041289292	24.691094348592664	24.967672924163853
26	23.188186922435573	27.195582606737435	24.536406944937184	25.07982352588981
27	22.23227059840165	25.429858511515768	26.78361904183173	25.554251848250853
28	24.678226072378692	24.86115219328151	28.568556188345095	21.892065545994704
29	22.500538412251313	24.63300837957125	28.04417393262564	24.822279275551796
30	22.714920319454958	25.17079508968027	30.86777756827112	21.246507022593654
31	27.299779898858922	24.68081760022734	25.311273771682824	22.708128729230914
32	26.767444333534996	28.107442957344347	23.108653826390867	22.01645888272979
33	24.797078901299425	24.398698516841804	25.25390270689552	25.55031987496325
34	20.95813252715966	23.86019690249868	28.306722512602423	26.87494805773924
35	23.534736749920242	25.34201465374954	27.65294259050911	23.470306005821108
36	25.435130930242327	26.13475408634792	22.82412194121521	25.60599304219454
37	24.641676593409834	27.977598475824173	25.002435142547437	22.37828978821856
38	22.770414760627723	25.416007241979894	28.997945543957226	22.815632453435157
39	22.210376656232043	27.061269973754076	25.264358181319373	25.463995188694504
40	24.849981814623547	23.263162503987825	27.62452514720325	24.26233053418538
41	21.07921943181199	23.11607095781976	27.2924521304593	28.51225747990896
42	22.20742767626634	25.049976274115732	23.831868822222084	28.910727227395842
43	22.272841413687377	22.08348115467757	28.069106217790214	27.574571213844834
44	26.30418638983192	22.569437307813637	25.982211395394767	25.14416490695968
45	21.335780688828102	24.975626233768324	27.20192738181516	26.486665695588414
46	21.947470624138205	25.659253407635713	25.779446681995406	26.61382928623068
47	23.246272891456986	24.67885161358354	28.702779458299187	23.372096036660288
48	22.539590056039554	20.740801639990313	32.60311823354313	24.116490070427005
49	21.97865832135124	21.368576920567882	27.681538759873497	28.971225998207377
50	19.614201930062706	23.981551896238802	30.935604107482277	25.46864206621622
51	22.401792264914913	23.75644642552351	27.398347320136796	26.44341398942478
52	21.292528982664464	22.450763204951425	29.07452966003623	27.18217815234788
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	213.0
1	182.0
2	151.0
3	122.5
4	94.0
5	132.5
6	171.0
7	224.5
8	278.0
9	302.0
10	326.0
11	539.5
12	753.0
13	998.0
14	1249.5
15	1256.0
16	1098.5
17	941.0
18	970.5
19	1000.0
20	1051.5
21	1103.0
22	1081.0
23	1059.0
24	2342.5
25	3626.0
26	4449.5
27	5273.0
28	6724.5
29	8176.0
30	9721.0
31	11266.0
32	14542.0
33	17818.0
34	19203.5
35	20589.0
36	22805.5
37	25022.0
38	27007.0
39	36423.5
40	43855.0
41	57371.5
42	70888.0
43	81206.5
44	91525.0
45	103864.0
46	116203.0
47	116051.5
48	115900.0
49	101014.5
50	86129.0
51	77027.5
52	67926.0
53	63569.5
54	59213.0
55	59354.5
56	59496.0
57	60035.0
58	60574.0
59	57806.5
60	55039.0
61	50464.0
62	45889.0
63	39491.5
64	29920.5
65	26747.0
66	23219.5
67	19692.0
68	15631.0
69	11570.0
70	10550.0
71	9530.0
72	7894.5
73	6259.0
74	5613.5
75	4968.0
76	3720.5
77	2473.0
78	1821.5
79	1170.0
80	914.5
81	659.0
82	636.5
83	614.0
84	380.5
85	147.0
86	92.5
87	38.0
88	37.0
89	33.0
90	30.0
91	19.0
92	8.0
93	7.0
94	6.0
95	4.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1119031.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.454965887083738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.40260338521955	11.255625052915063
2	12.257250748398953	4.0338528586867275
3	5.306505499858446	2.619551009393789
4	2.9225952741534886	1.9236482215179116
5	1.7601137654647043	1.4481305984054107
6	1.1912978465625026	1.1761659255925376
7	0.8418096497459874	0.9696364348991691
8	0.6125933751801607	0.8064162472994426
9	0.46058221293243695	0.682097814180071
>10	3.262028715379648	11.708542286808873
>50	1.2769441864490592	15.508288615915161
>100	1.6926175390041611	44.214343560996014
>500	0.005440750687895086	0.573512312806239
>1k	0.00761705096305312	3.080189060583618
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTT	4839	0.43242769860709845	TruSeq Adapter, Index 10 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	3624	0.3238516180516894	Illumina PCR Primer Index 2 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	3446	0.3079449988427488	TruSeq Adapter, Index 10 (95% over 23bp)
GTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	3406	0.3043704776722003	RNA PCR Primer, Index 10 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	3170	0.2832808027659645	Illumina PCR Primer Index 2 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	3071	0.27443386286885707	TruSeq Adapter, Index 10 (95% over 23bp)
ATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	2402	0.2146499962914343	TruSeq Adapter, Index 10 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	2387	0.21330955085247863	RNA PCR Primer, Index 10 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1615	0.14432129226089357	TruSeq Adapter, Index 10 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1455	0.13002320757869978	TruSeq Adapter, Index 10 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1307	0.11679747924767053	TruSeq Adapter, Index 10 (95% over 23bp)
TTTCCTGGGGTTTTATCCATTCACTCATAGCAGGTGATTAGACGATCTTGAT	1300	0.11617193804282454	No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	1225	0.10946971084804621	RNA PCR Primer, Index 10 (95% over 24bp)
CTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1158	0.10348238788737757	TruSeq Adapter, Index 10 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4456534269381277	0.0
2	0.0	0.0	0.0	1.008729874328772	0.0
3	0.0	0.0	0.0	3.4272508983218515	0.0
4	0.0	0.0	0.0	3.946986276519596	0.0
5	0.0	0.0	0.0	4.788339197037437	0.0
6	0.0	0.0	0.0	7.360743357422627	0.0
7	0.0	0.0	0.0	7.70899108246331	0.0
8	0.0	0.0	0.0	8.391099084833217	0.0
9	0.0	0.0	0.0	8.821292707708723	0.0
10	0.0	0.0	0.0	10.172014895029717	0.0
11	0.0	0.0	0.0	11.304065749742412	0.0
12	0.0	0.0	0.0	11.796277314926932	0.0
13	0.0	0.0	0.0	12.032999979446503	0.0
14	0.0	0.0	0.0	12.161414652498456	0.0
15	0.0	0.0	0.0	12.367932613126893	0.0
16	0.0	0.0	0.0	12.860144178311414	0.0
17	0.0	0.0	0.0	13.50775805138553	0.0
18	0.0	0.0	0.0	14.278514178785038	0.0
19	0.0	0.0	0.0	14.734712443176283	0.0
20	8.93630292637112E-5	0.0	0.0	15.228085727741233	0.0
21	8.93630292637112E-5	0.0	0.0	15.956841231386798	0.0
22	8.93630292637112E-5	0.0	0.0	16.560220404975375	0.0
23	8.93630292637112E-5	0.0	0.0	17.195055364864782	0.0
24	8.93630292637112E-5	0.0	0.0	17.69691813720978	0.0
25	8.93630292637112E-5	0.0	0.0	18.155886655508205	0.0
26	1.787260585274224E-4	0.0	0.0	18.555250033287727	0.0
27	1.787260585274224E-4	0.0	0.0	18.919315014508086	0.0
28	1.787260585274224E-4	0.0	0.0	19.290797127157337	0.0
29	1.787260585274224E-4	0.0	0.0	19.686585983766314	0.0
30	1.787260585274224E-4	0.0	0.0	20.117226421788136	0.0
31	1.787260585274224E-4	0.0	0.0	20.52356011585023	0.0
32	1.787260585274224E-4	0.0	0.0	20.94151100371661	0.0
33	1.787260585274224E-4	0.0	0.0	21.3439127244911	0.0
34	1.787260585274224E-4	0.0	0.0	21.728799291529903	0.0
35	1.787260585274224E-4	0.0	0.0	22.177580424492263	0.0
36	1.787260585274224E-4	0.0	0.0	22.593297236627045	0.0
37	1.787260585274224E-4	0.0	0.0	23.0189333450101	0.0
38	1.787260585274224E-4	0.0	0.0	23.425445765130725	0.0
39	1.787260585274224E-4	0.0	0.0	23.862073526113218	0.0
40	1.787260585274224E-4	0.0	0.0	24.303616253705215	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTG	35	1.01954356E-7	46.000004	1
GGTCGAA	35	1.01954356E-7	46.000004	9
ACGTTAT	35	1.01954356E-7	46.000004	38
GACGTTA	35	1.01954356E-7	46.000004	20
TAAGTCG	35	1.01954356E-7	46.000004	29
TACGGCA	35	1.01954356E-7	46.000004	27
TAACGCG	35	1.01954356E-7	46.000004	1
TTATCCG	35	1.01954356E-7	46.000004	26
TGCGAAT	35	1.01954356E-7	46.000004	4
ACTACGC	35	1.01954356E-7	46.000004	45
CGGGATA	55	1.8189894E-12	46.000004	6
GACGGTA	35	1.01954356E-7	46.000004	9
GAACGTT	35	1.01954356E-7	46.000004	23
TTGCGAA	35	1.01954356E-7	46.000004	3
CGAATCT	35	1.01954356E-7	46.000004	6
CGCACGG	35	1.01954356E-7	46.000004	1
TGTCGTA	35	1.01954356E-7	46.000004	21
AAAGCGT	75	0.0	46.000004	27
TACGTTT	35	1.01954356E-7	46.000004	43
TAGTATG	35	1.01954356E-7	46.000004	1
>>END_MODULE