FastQCFastQC Report
Thu 26 May 2016
SRR1545592_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545592_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1098396
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC68640.6249112341996875TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34920.3179181278883026No Hit
ATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG30030.2733986649623633No Hit
ATGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT27150.24717861317776102No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT24330.22150481247200463TruSeq Adapter, Index 16 (95% over 22bp)
ATTGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC22140.20156664809413No Hit
ATGCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC20640.18791037112298295No Hit
ATATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC20270.18454182280343337No Hit
ATGTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC19550.1779868098572828No Hit
ATGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC17460.1589590639441513No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC17440.15877698025120265TruSeq Adapter, Index 13 (95% over 21bp)
ATAGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC17000.15477113900633285No Hit
ATTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC14780.1345598490890353No Hit
ATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC11810.10752042068616419No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA351.2118653E-745.00000438
CGGGTCG253.8906917E-545.0000043
AACGTAA253.8906917E-545.00000429
ACTATGC253.8906917E-545.00000420
TCGTTAC351.2118653E-745.0000042
ACCGGTA253.8906917E-545.0000042
TACCCGC253.8906917E-545.00000442
CGAACAG253.8906917E-545.0000043
TCACCGA253.8906917E-545.00000414
GTCGAGA253.8906917E-545.00000433
GTCGAAC253.8906917E-545.00000445
TTAGCGA700.045.0000042
CGTTGAA351.2118653E-745.00000441
GCTATGC253.8906917E-545.00000434
CCGGATC351.2118653E-745.00000440
CCGGATA253.8906917E-545.00000421
TCTTACG253.8906917E-545.00000415
CTCCCGT253.8906917E-545.00000415
GGTCCGT502.1827873E-1145.00000410
CCGTGCA253.8906917E-545.00000442