##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545592_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1098396
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90405463967458	31.0	31.0	34.0	31.0	34.0
2	32.604921175969324	34.0	31.0	34.0	31.0	34.0
3	32.5445540588276	34.0	31.0	34.0	31.0	34.0
4	36.00602605981813	37.0	35.0	37.0	35.0	37.0
5	35.753896591029104	37.0	35.0	37.0	35.0	37.0
6	35.7449335212437	37.0	35.0	37.0	35.0	37.0
7	35.73704110357285	37.0	35.0	37.0	33.0	37.0
8	35.698596863062136	37.0	35.0	37.0	33.0	37.0
9	37.911478191836096	39.0	38.0	39.0	35.0	39.0
10	37.87157546094487	39.0	38.0	39.0	35.0	39.0
11	37.6952228522318	39.0	38.0	39.0	35.0	39.0
12	37.215670850949934	39.0	37.0	39.0	33.0	39.0
13	36.92241778010845	39.0	37.0	39.0	33.0	39.0
14	37.41367138991766	39.0	35.0	41.0	33.0	41.0
15	37.197670967483496	39.0	35.0	41.0	32.0	41.0
16	37.25058448865437	39.0	35.0	41.0	32.0	41.0
17	37.3920389367769	39.0	35.0	41.0	32.0	41.0
18	37.513126413424665	39.0	35.0	41.0	33.0	41.0
19	37.48972319636998	39.0	35.0	41.0	33.0	41.0
20	37.448807169727495	39.0	35.0	41.0	33.0	41.0
21	37.4076435092626	39.0	35.0	41.0	32.0	41.0
22	37.33630220794686	39.0	35.0	41.0	32.0	41.0
23	37.23994078638305	39.0	35.0	41.0	32.0	41.0
24	37.22943728855531	39.0	35.0	41.0	32.0	41.0
25	37.21215026274677	39.0	35.0	41.0	32.0	41.0
26	37.10379043623611	39.0	35.0	41.0	32.0	41.0
27	37.0385425657049	39.0	35.0	41.0	32.0	41.0
28	36.931144141093014	39.0	35.0	41.0	32.0	41.0
29	36.87612937410552	39.0	35.0	41.0	31.0	41.0
30	36.850210670832745	39.0	35.0	41.0	31.0	41.0
31	36.77354706317212	38.0	35.0	41.0	31.0	41.0
32	36.68141271454011	38.0	35.0	41.0	31.0	41.0
33	36.57404888582988	38.0	35.0	41.0	31.0	41.0
34	36.47422696368159	38.0	35.0	41.0	31.0	41.0
35	36.381580959872394	38.0	35.0	41.0	30.0	41.0
36	36.29212870403752	38.0	35.0	41.0	30.0	41.0
37	36.16744598487249	38.0	35.0	41.0	30.0	41.0
38	36.11737752140394	38.0	35.0	40.0	30.0	41.0
39	36.089878331676374	38.0	35.0	40.0	30.0	41.0
40	36.032919821266645	38.0	35.0	40.0	30.0	41.0
41	35.97884642697169	38.0	35.0	40.0	30.0	41.0
42	35.84387597915506	38.0	35.0	40.0	30.0	41.0
43	35.71768014450162	38.0	35.0	40.0	29.0	41.0
44	35.69175870997345	38.0	35.0	40.0	29.0	41.0
45	35.69715020812166	38.0	35.0	40.0	30.0	41.0
46	35.64172029031424	37.0	35.0	40.0	29.0	41.0
47	35.53974887017068	37.0	34.0	40.0	29.0	41.0
48	35.508196497438085	37.0	34.0	40.0	29.0	41.0
49	35.47662591633618	37.0	34.0	40.0	29.0	41.0
50	35.39870228952036	37.0	34.0	40.0	29.0	41.0
51	35.2503951216137	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	5.0
12	2.0
13	6.0
14	16.0
15	18.0
16	50.0
17	99.0
18	247.0
19	483.0
20	897.0
21	1444.0
22	2215.0
23	3632.0
24	5394.0
25	7793.0
26	10709.0
27	12933.0
28	15148.0
29	17914.0
30	21699.0
31	27451.0
32	34697.0
33	44770.0
34	73785.0
35	160109.0
36	75610.0
37	101754.0
38	152763.0
39	326593.0
40	154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.6317430143591198	91.66211457434295	2.9810742209549197	3.7250681903430096
2	3.477252284239928	2.209039362852742	91.28847883641237	3.025229516494962
3	26.224512835079516	16.709456334509596	44.58619659940495	12.47983423100594
4	26.258835611200332	15.470194720301238	44.51809729824217	13.752872370256263
5	24.764383701324476	14.189326982254125	46.43762358930659	14.608665727114811
6	21.99379822941817	14.243223755366916	49.221865338184045	14.54111267703087
7	22.889649998725414	17.466378246097037	44.91713371133908	14.72683804383847
8	24.21631178554911	18.306603447208474	44.69171409946868	12.785370667773735
9	80.71533399611798	3.984446410948328	10.293100120539405	5.007119472394291
10	82.11992760352368	3.930003386756689	10.003678090597562	3.9463909191220656
11	75.32920731685113	6.606269505715607	11.215171941631251	6.8493512358020245
12	44.68934701146034	23.21758272972589	16.51144031842796	15.581629940385799
13	37.409640967374244	23.362703433005947	24.413599466858944	14.81405613276086
14	32.404433373755914	25.175346596309527	24.57701958128034	17.84320044865422
15	21.273566181959875	32.376119359502404	29.67299589583356	16.677318562704162
16	15.671670326548895	37.2400300073926	28.5769431061293	18.511356559929208
17	14.743680785436217	24.99663145168045	42.266996602318294	17.99269116056504
18	18.043219385358285	22.279032334422194	40.4844882901977	19.19325999002181
19	18.17167943073354	21.392831000841227	29.966423767020274	30.46906580140496
20	20.355409160266426	23.909591804777147	33.16144632718983	22.5735527077666
21	25.76821110055026	25.188183496662408	28.83859737289648	20.20500802989086
22	28.46396017465468	23.337302757839616	26.33203325576568	21.86670381174003
23	22.99871813080164	27.601975972235877	28.572755181191482	20.826550715771
24	25.603698483971172	23.101868542857037	25.980884853914255	25.313548119257533
25	24.166602937374133	27.715778280328767	23.683534899981428	24.43408388231567
26	24.147484149614527	21.07773517019363	33.24793608134043	21.526844598851415
27	24.54324305623837	23.457751120725128	28.726342776193647	23.27266304684285
28	20.016642449535503	30.342153467419763	26.963772628450943	22.677431454593787
29	19.031660712529906	29.805097615067787	28.615544849034407	22.547696823367893
30	20.37680399418789	25.835855192480672	31.945127258293	21.842213555038438
31	17.9453494003984	26.39594463199065	31.54645501258198	24.11225095502897
32	19.992243234680387	27.958860010415187	31.35772526484073	20.69117149006369
33	25.02194108500031	26.011383872483147	24.517660297379088	24.449014745137454
34	26.385383777799625	26.759565766809057	25.952479797814267	20.902570657577048
35	26.481797093215924	27.32866834911999	24.767752249644026	21.421782308020056
36	22.097859059938312	25.63492583731186	26.350150583214067	25.91706451953576
37	20.013364943062427	24.379185648891657	29.69703094330278	25.910418464743135
38	24.794973761739847	27.827759751492177	24.006824496811717	23.370441989956266
39	22.154122921059436	29.734449142203722	26.30836237568236	21.80306556105448
40	22.397477776685275	27.743272917964013	28.257750392390356	21.601498912960356
41	20.740607212699246	29.130568574539602	25.911419925054354	24.2174042877068
42	25.671524659594535	24.4694081187477	26.88738851925899	22.97167870239877
43	20.243518730949496	24.34103911521892	27.869730042716835	27.54571211111475
44	19.710377677995915	26.279957319582376	26.069377528687287	27.940287473734426
45	20.521196362696152	23.17661389881245	28.405784434757592	27.896405303733808
46	22.91932964067604	25.48488887432219	28.13447973226414	23.46130175273763
47	19.456644051872	28.434371574550525	26.835676750461584	25.273307623115887
48	20.408304473068	28.24054348340671	25.512110386418012	25.83904165710727
49	21.369069078911433	26.1866394269462	28.746280940571523	23.698010553570846
50	21.198183533079145	22.959752220510634	31.533709154075577	24.30835509233464
51	21.184345172415046	22.164865858943404	29.109355824311084	27.541433144330462
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	681.0
1	896.5
2	1112.0
3	3287.5
4	5463.0
5	3695.0
6	1927.0
7	2000.0
8	2073.0
9	2256.5
10	2440.0
11	2556.5
12	2673.0
13	2664.0
14	2655.0
15	2865.5
16	3076.0
17	3355.0
18	3634.0
19	3588.0
20	3542.0
21	3882.0
22	4222.0
23	4818.0
24	5414.0
25	6412.0
26	8128.0
27	8846.0
28	11351.0
29	13856.0
30	15397.0
31	16938.0
32	20585.0
33	24232.0
34	29209.0
35	34186.0
36	38269.0
37	42352.0
38	52009.5
39	61667.0
40	73920.5
41	86174.0
42	91704.0
43	97234.0
44	105672.0
45	114110.0
46	120444.0
47	126778.0
48	114805.5
49	102833.0
50	88752.5
51	74672.0
52	62611.5
53	50551.0
54	47765.0
55	44979.0
56	44288.5
57	43598.0
58	40240.5
59	36883.0
60	32555.5
61	28228.0
62	22761.5
63	17295.0
64	13242.5
65	9190.0
66	8177.0
67	7164.0
68	6102.5
69	5041.0
70	3574.5
71	2108.0
72	1839.5
73	1571.0
74	1282.0
75	603.0
76	213.0
77	183.5
78	154.0
79	89.5
80	25.0
81	109.5
82	194.0
83	99.5
84	5.0
85	4.0
86	3.0
87	1.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1098396.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.716655865857366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.71671544356285	11.654303401679437
2	12.122741379451533	4.053033915821606
3	5.426961730714295	2.721619549485857
4	2.974076940801634	1.988664829518511
5	1.8375757470506973	1.5359060695446136
6	1.145961347328629	1.1493984887320163
7	0.7870869770104979	0.9210223491827738
8	0.5563156085075525	0.743978926418063
9	0.4319672394189765	0.6498942918022302
>10	2.7077956430538297	9.272358563051297
>50	0.7199857317744445	8.86250028235102
>100	1.5514954493362358	51.733909817140635
>500	0.01257378271132256	1.3626812719524601
>1k	0.008200293072601669	2.7234434032228063
>5k	5.466862048401113E-4	0.6272848400966862
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	6864	0.6249112341996875	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3492	0.3179181278883026	No Hit
ATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	3003	0.2733986649623633	No Hit
ATGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT	2715	0.24717861317776102	No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	2433	0.22150481247200463	TruSeq Adapter, Index 16 (95% over 22bp)
ATTGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	2214	0.20156664809413	No Hit
ATGCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	2064	0.18791037112298295	No Hit
ATATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	2027	0.18454182280343337	No Hit
ATGTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	1955	0.1779868098572828	No Hit
ATGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	1746	0.1589590639441513	No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	1744	0.15877698025120265	TruSeq Adapter, Index 13 (95% over 21bp)
ATAGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	1700	0.15477113900633285	No Hit
ATTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	1478	0.1345598490890353	No Hit
ATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	1181	0.10752042068616419	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.104184647431345E-5	0.0	0.0	0.23862067960917557	0.0
2	9.104184647431345E-5	0.0	0.0	1.1124403220696362	0.0
3	9.104184647431345E-5	0.0	0.0	1.5141169487143071	0.0
4	9.104184647431345E-5	0.0	0.0	2.0153023135554027	0.0
5	9.104184647431345E-5	0.0	0.0	3.914344189163107	0.0
6	9.104184647431345E-5	0.0	0.0	4.329495009085976	0.0
7	9.104184647431345E-5	0.0	0.0	5.028332222622806	0.0
8	9.104184647431345E-5	0.0	0.0	7.057199771302882	0.0
9	9.104184647431345E-5	0.0	0.0	7.317761535912367	0.0
10	9.104184647431345E-5	0.0	0.0	7.835425474965313	0.0
11	9.104184647431345E-5	0.0	0.0	8.23446188806223	0.0
12	9.104184647431345E-5	0.0	0.0	9.251945564259156	0.0
13	9.104184647431345E-5	0.0	0.0	10.170011544106133	0.0
14	9.104184647431345E-5	0.0	0.0	10.5688658735101	0.0
15	9.104184647431345E-5	0.0	0.0	10.77571294869974	0.0
16	1.820836929486269E-4	0.0	0.0	10.909999672249352	0.0
17	1.820836929486269E-4	0.0	0.0	11.087076063641893	0.0
18	2.731255394229404E-4	0.0	0.0	11.509328147589757	0.0
19	2.731255394229404E-4	0.0	0.0	12.055124017203267	0.0
20	2.731255394229404E-4	0.0	0.0	12.677303996008725	0.0
21	4.552092323715673E-4	0.0	0.0	13.077706036802756	0.0
22	4.552092323715673E-4	0.0	0.0	13.493221024111522	0.0
23	4.552092323715673E-4	0.0	0.0	14.136886878684919	0.0
24	4.552092323715673E-4	0.0	0.0	14.683502124916696	0.0
25	7.283347717945076E-4	0.0	0.0	15.25852242724846	0.0
26	8.19376618268821E-4	0.0	0.0	15.690333905076129	0.0
27	9.104184647431346E-4	0.0	0.0	16.077808003670807	0.0
28	0.0010014603112174479	0.0	0.0	16.42249243442256	0.0
29	0.0010014603112174479	0.0	0.0	16.772366250423346	0.0
30	0.0010014603112174479	0.0	0.0	17.1131358817767	0.0
31	0.0010014603112174479	0.0	0.0	17.481127025225874	0.0
32	0.0010014603112174479	0.0	0.0	17.87825155954683	0.0
33	0.0010014603112174479	0.0	0.0	18.285026529594063	0.0
34	0.0010925021576917615	0.0	0.0	18.663942694620154	0.0
35	0.0010925021576917615	0.0	0.0	19.02892945713568	0.0
36	0.0010925021576917615	0.0	0.0	19.396374349506008	0.0
37	0.0010925021576917615	0.0	0.0	19.850308996026932	0.0
38	0.0010925021576917615	0.0	0.0	20.235689132152704	0.0
39	0.0013656276971147018	0.0	0.0	20.657212881328775	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	35	1.2118653E-7	45.000004	38
CGGGTCG	25	3.8906917E-5	45.000004	3
AACGTAA	25	3.8906917E-5	45.000004	29
ACTATGC	25	3.8906917E-5	45.000004	20
TCGTTAC	35	1.2118653E-7	45.000004	2
ACCGGTA	25	3.8906917E-5	45.000004	2
TACCCGC	25	3.8906917E-5	45.000004	42
CGAACAG	25	3.8906917E-5	45.000004	3
TCACCGA	25	3.8906917E-5	45.000004	14
GTCGAGA	25	3.8906917E-5	45.000004	33
GTCGAAC	25	3.8906917E-5	45.000004	45
TTAGCGA	70	0.0	45.000004	2
CGTTGAA	35	1.2118653E-7	45.000004	41
GCTATGC	25	3.8906917E-5	45.000004	34
CCGGATC	35	1.2118653E-7	45.000004	40
CCGGATA	25	3.8906917E-5	45.000004	21
TCTTACG	25	3.8906917E-5	45.000004	15
CTCCCGT	25	3.8906917E-5	45.000004	15
GGTCCGT	50	2.1827873E-11	45.000004	10
CCGTGCA	25	3.8906917E-5	45.000004	42
>>END_MODULE