FastQCFastQC Report
Thu 26 May 2016
SRR1545591_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545591_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences921350
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTT51710.561241656265263TruSeq Adapter, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT40770.4425028490801541TruSeq Adapter, Index 13 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC40720.44196016714603575TruSeq Adapter, Index 13 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC39850.4325175014923753TruSeq Adapter, Index 16 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC38240.4150431432137624TruSeq Adapter, Index 16 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC30190.3276713518206979TruSeq Adapter, Index 13 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC29940.3249579421501058TruSeq Adapter, Index 16 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC29820.32365550550822164TruSeq Adapter, Index 13 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC24460.2654800021707277TruSeq Adapter, Index 16 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC15250.16551798990611602TruSeq Adapter, Index 16 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC15060.16345579855646605TruSeq Adapter, Index 16 (95% over 21bp)
CTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC14380.15607532425245565TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT11980.13002659141477182TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT10630.11537417919357464TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG750.046.0000041
TAATCAC301.8609971E-646.031
TCACGCC206.310799E-446.016
TCTGCGA253.416624E-546.021
AACGTTC253.416624E-546.039
ATCCTAC253.416624E-546.012
GAAATCG501.6370905E-1146.05
AGCGTAC253.416624E-546.043
AGCCGAC206.310799E-446.044
TCGTTCG206.310799E-446.036
GCCCATG301.8609971E-646.034
GTCGCAG1350.046.040
TACCCGC301.8609971E-646.040
TCGCAAT206.310799E-446.011
AAATCGC501.6370905E-1146.06
CAAACGT301.8609971E-646.029
GCCCACG253.416624E-546.039
ACAAACG301.8609971E-646.014
ACCGGCA206.310799E-446.04
AGATCAA206.310799E-446.024
Produced by FastQC (version 0.11.5)