##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545591_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	921350
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.567101535789874	33.0	31.0	34.0	31.0	34.0
2	32.959394366961526	34.0	31.0	34.0	31.0	34.0
3	33.01494980192109	34.0	33.0	34.0	31.0	34.0
4	36.381423997395125	37.0	37.0	37.0	35.0	37.0
5	36.3662223910566	37.0	37.0	37.0	35.0	37.0
6	36.36581212351441	37.0	37.0	37.0	35.0	37.0
7	36.47976447604059	37.0	37.0	37.0	35.0	37.0
8	36.5309209312422	37.0	37.0	37.0	35.0	37.0
9	38.331880392901724	39.0	39.0	39.0	37.0	39.0
10	38.09660932327563	39.0	39.0	39.0	35.0	39.0
11	37.89769577250773	39.0	38.0	39.0	35.0	39.0
12	37.406263634883594	39.0	37.0	39.0	35.0	39.0
13	37.281794106474194	39.0	37.0	39.0	35.0	39.0
14	38.54664351223748	40.0	37.0	41.0	35.0	41.0
15	38.602680848754545	40.0	37.0	41.0	35.0	41.0
16	38.70289792152819	40.0	37.0	41.0	35.0	41.0
17	38.67314918326369	40.0	37.0	41.0	35.0	41.0
18	38.68502849080154	40.0	36.0	41.0	35.0	41.0
19	38.64341781082108	40.0	36.0	41.0	35.0	41.0
20	38.57223422152276	40.0	36.0	41.0	35.0	41.0
21	38.502164215553265	40.0	36.0	41.0	35.0	41.0
22	38.60384870027677	40.0	36.0	41.0	35.0	41.0
23	38.63494546046562	40.0	36.0	41.0	35.0	41.0
24	38.588597167200305	40.0	36.0	41.0	35.0	41.0
25	38.540168231399576	40.0	36.0	41.0	35.0	41.0
26	38.44002170727737	40.0	36.0	41.0	35.0	41.0
27	38.37794866228903	40.0	36.0	41.0	35.0	41.0
28	38.37737124871113	40.0	36.0	41.0	35.0	41.0
29	38.33913170890541	40.0	36.0	41.0	35.0	41.0
30	38.2895436044934	40.0	36.0	41.0	35.0	41.0
31	38.209581592228794	40.0	35.0	41.0	35.0	41.0
32	38.14244206870353	40.0	35.0	41.0	34.0	41.0
33	38.10509361263364	40.0	35.0	41.0	34.0	41.0
34	38.01136810115591	40.0	35.0	41.0	34.0	41.0
35	37.952560916047105	40.0	35.0	41.0	34.0	41.0
36	37.920329950615944	40.0	35.0	41.0	34.0	41.0
37	37.88125685135942	40.0	35.0	41.0	34.0	41.0
38	37.81309165897867	40.0	35.0	41.0	34.0	41.0
39	37.74472893037391	40.0	35.0	41.0	34.0	41.0
40	37.615743202908774	40.0	35.0	41.0	34.0	41.0
41	37.4854398437076	39.0	35.0	41.0	34.0	41.0
42	37.49664731101102	39.0	35.0	41.0	34.0	41.0
43	37.42823031421284	39.0	35.0	41.0	33.0	41.0
44	37.34040049926738	39.0	35.0	41.0	33.0	41.0
45	37.248549411190105	39.0	35.0	41.0	33.0	41.0
46	37.279792695501165	39.0	35.0	41.0	33.0	41.0
47	37.26317686004233	39.0	35.0	41.0	33.0	41.0
48	37.200891083735826	39.0	35.0	41.0	33.0	41.0
49	37.20245509306995	39.0	35.0	41.0	33.0	41.0
50	37.10902480056439	39.0	35.0	41.0	33.0	41.0
51	36.98475714983448	38.0	35.0	41.0	33.0	41.0
52	36.66057849894177	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	3.0
13	2.0
14	7.0
15	15.0
16	29.0
17	40.0
18	101.0
19	164.0
20	320.0
21	459.0
22	771.0
23	1103.0
24	1640.0
25	2230.0
26	2864.0
27	3602.0
28	4840.0
29	6554.0
30	8310.0
31	10890.0
32	14461.0
33	20622.0
34	48600.0
35	129500.0
36	47789.0
37	67594.0
38	120008.0
39	428208.0
40	619.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.477994247571495	14.57535138655234	47.30666956097031	11.639984804905845
2	24.95284093992511	13.21332826830195	49.73690779833939	12.096922993433548
3	25.525587453193683	14.115591251967222	44.748792532696584	15.610028762142509
4	22.53313073207793	14.199055733434635	49.051826124708306	14.21598740977913
5	23.603408042546263	17.200846583817224	44.07673522548434	15.119010148152167
6	23.388180387474904	17.89330873175232	45.53904596515982	13.179464915612959
7	83.38155966787866	2.6123622944592175	8.052097465675367	5.953980571986758
8	85.4477668638411	2.6262549519726486	8.222282520214902	3.7036956639713465
9	77.22483312530525	4.207521571606882	10.315840885656916	8.251804417430945
10	44.366418841916754	22.672708525533185	15.092310196993541	17.86856243555652
11	36.99191403918164	21.426276659250014	25.973300048841374	15.608509252726977
12	32.391490747273025	25.243609920225758	23.399793780865036	18.965105551636185
13	21.661583545883758	30.23009714006621	31.47012535952678	16.638193954523253
14	15.446138818038749	37.981765887013616	28.034514571009932	18.5375807239377
15	14.571769685787162	24.75660715254789	40.90215444727845	19.769468714386498
16	17.963640310414068	23.648016497530797	38.60281109241873	19.785532099636402
17	18.266782438812612	22.405600477560103	27.810278395832206	31.517338687795082
18	19.826016171921637	24.761925435502253	31.066478536929505	24.34557985564661
19	24.385955391545018	26.355890812394854	28.602811092418733	20.655342703641395
20	27.883323384164544	22.272534867314267	25.108265045856626	24.73587670266457
21	22.37640418950453	28.488956422640687	28.48776252238563	20.64687686546915
22	25.60981168936886	22.512183209420957	25.559450805882673	26.318554295327505
23	24.432843110652847	28.476149128995498	21.374179193574648	25.71682856677701
24	24.64514028327997	20.265045856623434	30.983556737396214	24.106257122700388
25	26.88240082487654	23.01481521680143	26.400716340153036	23.70206761816899
26	19.818852770391274	29.375481630216534	25.965051283442776	24.840614315949423
27	18.815325337819505	28.100287621425085	27.781299180550278	25.30308786020513
28	20.05709013946926	24.83290823248494	30.86818255820264	24.241819069843164
29	18.178650892711783	26.632658598795246	29.955608617789114	25.233081890703858
30	20.33678840831389	26.005860964888477	32.28707874314864	21.370271883648996
31	25.413360829217996	25.86465512563087	22.39073099256526	26.33125305258588
32	25.47034243230043	27.788679654854292	23.33890486785695	23.402073044988335
33	25.19324903673957	27.46600097682748	24.966407988278068	22.374341998154883
34	21.622944592174527	24.855483800944267	25.249253812340587	28.27231779454062
35	19.104249199544146	23.302545178271014	29.354425571172737	28.238780051012103
36	23.718131003418897	27.665382319422587	22.880121560753242	25.736365116405274
37	22.860367938351335	28.268301948228142	25.110652846366744	23.76067726705378
38	21.41824496662506	26.311607966570794	29.93932815976556	22.330818907038584
39	21.06810658273186	28.435773593097085	24.541922179410648	25.954197644760406
40	26.127964400065125	23.39545232539209	27.613284853747217	22.863298420795573
41	20.182883811797904	23.43593639767732	27.72475172301514	28.65642806750963
42	19.974059803549142	25.809735713898085	24.620502523470993	29.595701959081783
43	20.586964779942477	21.63629456775384	28.481033266402562	29.29570738590112
44	25.71194442936995	23.93726596841591	25.722580995278665	24.628208606935477
45	19.718565148966192	25.748846800889996	27.194551473381452	27.33803657676236
46	20.219894719704783	26.43479676561567	24.749335214630705	28.595973300048843
47	21.443968090302274	24.847126499158843	29.46632658598795	24.242578824550932
48	19.988712215770338	20.916698323112822	33.89667335974386	25.197916101372986
49	20.583166006403648	20.314646985401854	28.103326640256142	30.998860367938352
50	18.838877733760242	23.814836924078797	31.059423671785968	26.286861670374996
51	21.065935854995388	24.531068540728278	27.814945460465623	26.588050143810715
52	20.16530091713247	20.73348890215445	29.729961469582676	29.371248711130406
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	371.0
1	284.5
2	198.0
3	171.0
4	144.0
5	155.0
6	166.0
7	265.5
8	365.0
9	436.0
10	507.0
11	520.0
12	533.0
13	660.0
14	855.0
15	923.0
16	924.0
17	925.0
18	1241.0
19	1557.0
20	1552.0
21	1547.0
22	1474.5
23	1402.0
24	2314.0
25	3226.0
26	3533.5
27	3841.0
28	5257.5
29	6674.0
30	7717.5
31	8761.0
32	10376.5
33	11992.0
34	14372.5
35	16753.0
36	18991.5
37	21230.0
38	24772.0
39	36881.5
40	45449.0
41	55327.0
42	65205.0
43	68578.5
44	71952.0
45	79957.0
46	87962.0
47	98943.0
48	109924.0
49	110756.0
50	111588.0
51	94818.0
52	78048.0
53	66189.5
54	54331.0
55	49085.5
56	43840.0
57	38852.0
58	33864.0
59	32974.5
60	32085.0
61	29479.0
62	26873.0
63	22114.5
64	15091.0
65	12826.0
66	10224.0
67	7622.0
68	6446.0
69	5270.0
70	3714.0
71	2158.0
72	2306.0
73	2454.0
74	1786.0
75	1118.0
76	854.5
77	591.0
78	413.5
79	236.0
80	166.5
81	97.0
82	109.5
83	122.0
84	138.0
85	154.0
86	80.0
87	6.0
88	4.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	921350.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.860502087624134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.50474189477941	10.875823353785844
2	13.034005554283562	4.395197557162051
3	6.293035315727044	3.183112052349247
4	3.6443524908081373	2.4578245111723525
5	2.302401463779499	1.940982234330155
6	1.5231717682060104	1.5408864466588548
7	1.1002516707793506	1.2985556914461027
8	0.8089978487090803	1.0912087933634307
9	0.6186541309130036	0.9387737339198393
>10	3.716380053604769	12.515011079397523
>50	0.9094643111483086	11.377260059502946
>100	1.5258646976702146	43.01280867472504
>500	0.009661448064606677	1.1046579423019516
>1k	0.008373254989325788	3.706338076368788
>5k	6.440965376404452E-4	0.5615597935158663
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTT	5171	0.561241656265263	TruSeq Adapter, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	4077	0.4425028490801541	TruSeq Adapter, Index 13 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	4072	0.44196016714603575	TruSeq Adapter, Index 13 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	3985	0.4325175014923753	TruSeq Adapter, Index 16 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	3824	0.4150431432137624	TruSeq Adapter, Index 16 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	3019	0.3276713518206979	TruSeq Adapter, Index 13 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	2994	0.3249579421501058	TruSeq Adapter, Index 16 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	2982	0.32365550550822164	TruSeq Adapter, Index 13 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	2446	0.2654800021707277	TruSeq Adapter, Index 16 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	1525	0.16551798990611602	TruSeq Adapter, Index 16 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	1506	0.16345579855646605	TruSeq Adapter, Index 16 (95% over 21bp)
CTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	1438	0.15607532425245565	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	1198	0.13002659141477182	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	1063	0.11537417919357464	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5805611331198784	0.0
2	0.0	0.0	0.0	1.3267487925326966	0.0
3	0.0	0.0	0.0	4.803820480816194	0.0
4	0.0	0.0	0.0	5.508330167688718	0.0
5	0.0	0.0	0.0	6.708742605958648	0.0
6	0.0	0.0	0.0	10.281217778260162	0.0
7	0.0	0.0	0.0	10.745536441091875	0.0
8	0.0	0.0	0.0	11.643892114831496	0.0
9	0.0	0.0	0.0	12.264177565528843	0.0
10	0.0	0.0	0.0	14.062734031584089	0.0
11	0.0	0.0	0.0	15.598198295978726	0.0
12	0.0	0.0	0.0	16.278070223042274	0.0
13	0.0	0.0	0.0	16.60563303847615	0.0
14	0.0	0.0	0.0	16.7951375698703	0.0
15	0.0	0.0	0.0	17.06213708145656	0.0
16	0.0	0.0	0.0	17.705106637000053	0.0
17	0.0	0.0	0.0	18.59836110055896	0.0
18	0.0	0.0	0.0	19.60481901557497	0.0
19	3.2560916047104794E-4	0.0	0.0	20.24550930699517	0.0
20	3.2560916047104794E-4	0.0	0.0	20.911705649318936	0.0
21	3.2560916047104794E-4	0.0	0.0	21.938134259510502	0.0
22	3.2560916047104794E-4	0.0	0.0	22.772996146958267	0.0
23	3.2560916047104794E-4	0.0	0.0	23.65290063493786	0.0
24	3.2560916047104794E-4	0.0	0.0	24.308677484126555	0.0
25	3.2560916047104794E-4	0.0	0.0	24.892928854398438	0.0
26	3.2560916047104794E-4	0.0	0.0	25.40077060834645	0.0
27	3.2560916047104794E-4	0.0	0.0	25.914147718022466	0.0
28	3.2560916047104794E-4	0.0	0.0	26.40115048570033	0.0
29	3.2560916047104794E-4	0.0	0.0	26.897378846258206	0.0
30	3.2560916047104794E-4	0.0	0.0	27.440060780376623	0.0
31	3.2560916047104794E-4	0.0	0.0	27.988060997449395	0.0
32	3.2560916047104794E-4	0.0	0.0	28.505996635372007	0.0
33	3.2560916047104794E-4	0.0	0.0	29.0091713246866	0.0
34	3.2560916047104794E-4	0.0	0.0	29.498887502035057	0.0
35	3.2560916047104794E-4	0.0	0.0	30.085635209203886	0.0
36	3.2560916047104794E-4	0.0	0.0	30.56677701199327	0.0
37	4.3414554729473057E-4	0.0	0.0	31.115971129321103	0.0
38	4.3414554729473057E-4	0.0	0.0	31.630867748412655	0.0
39	4.3414554729473057E-4	0.0	0.0	32.18212405709014	0.0
40	4.3414554729473057E-4	0.0	0.0	32.6961523850871	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	75	0.0	46.000004	1
TAATCAC	30	1.8609971E-6	46.0	31
TCACGCC	20	6.310799E-4	46.0	16
TCTGCGA	25	3.416624E-5	46.0	21
AACGTTC	25	3.416624E-5	46.0	39
ATCCTAC	25	3.416624E-5	46.0	12
GAAATCG	50	1.6370905E-11	46.0	5
AGCGTAC	25	3.416624E-5	46.0	43
AGCCGAC	20	6.310799E-4	46.0	44
TCGTTCG	20	6.310799E-4	46.0	36
GCCCATG	30	1.8609971E-6	46.0	34
GTCGCAG	135	0.0	46.0	40
TACCCGC	30	1.8609971E-6	46.0	40
TCGCAAT	20	6.310799E-4	46.0	11
AAATCGC	50	1.6370905E-11	46.0	6
CAAACGT	30	1.8609971E-6	46.0	29
GCCCACG	25	3.416624E-5	46.0	39
ACAAACG	30	1.8609971E-6	46.0	14
ACCGGCA	20	6.310799E-4	46.0	4
AGATCAA	20	6.310799E-4	46.0	24
>>END_MODULE