FastQCFastQC Report
Thu 26 May 2016
SRR1545590_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545590_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1718470
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC104940.6106594819810646TruSeq Adapter, Index 15 (95% over 23bp)
ATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG77700.45214638602943313TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63900.3718423946882983No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC50500.2938660552700949TruSeq Adapter, Index 21 (95% over 21bp)
ATGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT49270.2867085256070807TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT49230.28647576041478756TruSeq Adapter, Index 15 (95% over 24bp)
ATATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC41340.24056282623496483No Hit
ATGTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC36720.21367844652510665No Hit
ATTGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC35820.20844122969851087No Hit
ATGCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC31420.18283705854626497No Hit
ATTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC28100.163517547585934No Hit
ATGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC24670.14355793234679684No Hit
ATAGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC24420.14210314989496473No Hit
ATCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT22360.13011574249186778TruSeq Adapter, Index 15 (95% over 21bp)
GATTGGGGGCGATATCGCTGGGGGATGCATTCTTCATGAATACTTTTGTAT21110.122841830232707No Hit
ATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC21010.12225991725197413No Hit
ATTCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC19080.11102899672382992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC900.045.032
TCGCCTC253.8916158E-545.021
CGGAATA207.0342637E-445.044
AACCGTA253.8916158E-545.038
AACCGGT351.2123928E-745.021
CGGAACG253.8916158E-545.027
TGCCCCG406.8175723E-945.02
GTCGATA551.8189894E-1245.010
CGTATCA253.8916158E-545.020
CGACGAA453.8562575E-1045.021
TAGACGT406.8175723E-945.028
TCCGAAC502.1827873E-1145.035
TAGCGTC406.8175723E-945.034
AACCACG406.8175723E-945.016
TAGCGCG253.8916158E-545.03
CCGGATA253.8916158E-545.023
CCGTGTT351.2123928E-745.037
TGAACGT253.8916158E-545.014
GCGCCCG207.0342637E-445.035
TTCGTAA453.8562575E-1045.02
Produced by FastQC (version 0.11.5)