##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545590_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1718470
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.027438360867514	31.0	31.0	34.0	31.0	34.0
2	32.71309711545735	34.0	31.0	34.0	31.0	34.0
3	32.62926440380105	34.0	31.0	34.0	31.0	34.0
4	36.054759175312924	37.0	35.0	37.0	35.0	37.0
5	35.81415037795248	37.0	35.0	37.0	35.0	37.0
6	35.80183535354123	37.0	35.0	37.0	35.0	37.0
7	35.791566917083216	37.0	35.0	37.0	35.0	37.0
8	35.76003479839625	37.0	35.0	37.0	33.0	37.0
9	38.01258561394729	39.0	39.0	39.0	35.0	39.0
10	37.995169540346936	39.0	39.0	39.0	35.0	39.0
11	37.79263472740286	39.0	38.0	39.0	35.0	39.0
12	37.27682531554231	39.0	37.0	39.0	34.0	39.0
13	36.93139769678842	39.0	37.0	39.0	33.0	39.0
14	37.71754176680419	40.0	36.0	41.0	33.0	41.0
15	37.61258503203431	40.0	36.0	41.0	33.0	41.0
16	37.66358970479555	40.0	36.0	41.0	33.0	41.0
17	37.782799234202514	40.0	36.0	41.0	33.0	41.0
18	37.87590123772891	40.0	36.0	41.0	33.0	41.0
19	37.86484489109498	40.0	36.0	41.0	33.0	41.0
20	37.80458896576606	40.0	36.0	41.0	33.0	41.0
21	37.79207725476732	40.0	36.0	41.0	33.0	41.0
22	37.7108305643974	40.0	35.0	41.0	33.0	41.0
23	37.67158926254168	40.0	35.0	41.0	33.0	41.0
24	37.6343491594267	40.0	35.0	41.0	33.0	41.0
25	37.57280604258439	40.0	35.0	41.0	33.0	41.0
26	37.5036363742166	39.0	35.0	41.0	33.0	41.0
27	37.40574697259772	39.0	35.0	41.0	32.0	41.0
28	37.32278829423848	39.0	35.0	41.0	32.0	41.0
29	37.29504792053396	39.0	35.0	41.0	32.0	41.0
30	37.28002350928442	39.0	35.0	41.0	32.0	41.0
31	37.24373192432804	39.0	35.0	41.0	32.0	41.0
32	37.09908930618516	39.0	35.0	41.0	32.0	41.0
33	37.01105634663392	39.0	35.0	41.0	31.0	41.0
34	36.957849133240614	39.0	35.0	41.0	31.0	41.0
35	36.82664230390987	39.0	35.0	41.0	31.0	41.0
36	36.80185106519171	39.0	35.0	41.0	31.0	41.0
37	36.6761008338813	39.0	35.0	41.0	31.0	41.0
38	36.544858507858734	39.0	35.0	41.0	30.0	41.0
39	36.52707524716754	39.0	35.0	41.0	30.0	41.0
40	36.484117267103876	38.0	35.0	41.0	30.0	41.0
41	36.46056608494766	38.0	35.0	41.0	30.0	41.0
42	36.313262960656864	38.0	35.0	41.0	30.0	41.0
43	36.22719977654542	38.0	35.0	40.0	30.0	41.0
44	36.164613289728656	38.0	35.0	40.0	30.0	41.0
45	36.16765844035683	38.0	35.0	40.0	30.0	41.0
46	36.12376648995909	38.0	35.0	40.0	30.0	41.0
47	36.02234604037312	38.0	35.0	40.0	30.0	41.0
48	35.99405459507585	38.0	35.0	40.0	30.0	41.0
49	35.95525787473741	38.0	35.0	40.0	30.0	41.0
50	35.84158641116807	38.0	35.0	40.0	30.0	41.0
51	35.715974093234095	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	4.0
10	2.0
11	6.0
12	6.0
13	8.0
14	9.0
15	15.0
16	43.0
17	119.0
18	247.0
19	488.0
20	898.0
21	1552.0
22	2598.0
23	4218.0
24	6462.0
25	9977.0
26	14094.0
27	17694.0
28	20944.0
29	25362.0
30	31459.0
31	39735.0
32	50107.0
33	64825.0
34	102425.0
35	209912.0
36	119986.0
37	161490.0
38	246877.0
39	586562.0
40	341.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.3989188056817985	92.96391557606476	2.329572235767863	3.307593382485583
2	2.841830232707001	1.942716486176657	92.76583239742328	2.449620883693053
3	25.984742241645186	15.775428142475576	44.75376352220289	13.486066093676353
4	24.518670677986815	14.491728106978883	47.51494061578032	13.474660599253987
5	23.279312411621966	13.193247481771575	49.28500352057353	14.242436586032925
6	19.798018004387625	14.444651346837595	51.36825199159718	14.389078657177606
7	21.050352930222815	17.412058400786744	46.928546905095814	14.609041763894629
8	24.568016898752962	17.046908005376878	46.39219771075433	11.992877385115829
9	83.51417249064575	2.6160479961826506	9.52085285166456	4.348926661507038
10	84.8702625009456	2.9253929367402396	8.965882441939632	3.238462120374519
11	77.94142463935944	6.384516459408659	10.043876238747258	5.6301826624846525
12	40.73123185158891	29.48995327238765	14.916000861231213	14.862814014792228
13	29.105017835632857	23.781270548802134	31.084569413489906	16.029142202075104
14	26.745186124866887	25.35726547452094	28.71164466065744	19.18590373995473
15	19.890309403131855	30.390114462283307	33.593254464727345	16.12632166985749
16	14.941779606277677	36.33179514335426	30.607633534481256	18.118791715886807
17	13.642717068089638	26.08349287447555	41.520655001251114	18.753135056183698
18	17.390818576989997	23.26063300494044	38.63692703393134	20.711621384138216
19	17.96761072349241	23.50532741333861	30.661809632987485	27.865252230181497
20	19.457657101956976	23.156761537879625	35.79340925358022	21.59217210658318
21	22.836593015880403	26.284834765809123	30.645166921738525	20.233405296571952
22	24.192217495795678	24.578025802021568	28.862476505263402	22.367280196919353
23	22.040594249535925	26.943793025191017	30.708129906253816	20.307482819019242
24	26.54413519002368	22.50315687792048	26.758220975635304	24.194486956420537
25	22.12962693558805	26.490366430603967	26.615535912759608	24.764470721048372
26	21.86817343334478	23.10793903879614	35.23174684457686	19.792140683282224
27	22.604700693058362	27.044056631771284	29.965550751540615	20.38569192362974
28	19.613900737283746	29.91102550524595	27.141701629938257	23.333372127532048
29	17.306266620889513	28.750691021664622	29.26318178379605	24.67986057364982
30	17.878170698353767	29.33347687186858	31.807654483348557	20.980697946429093
31	17.258724330363638	23.172647762253632	33.110208499421	26.45841940796173
32	17.679621989327714	28.504425448218473	32.53871176104325	21.277240801410557
33	20.839584048601374	31.79613260633005	26.524990252957576	20.839293092111006
34	22.411621966051197	26.364615035467597	27.843663258596308	23.380099739884898
35	21.303543268139684	27.63824797639761	27.207341414164926	23.850867341297782
36	19.04944514597287	28.92264630747118	28.615978166624963	23.411930379930983
37	20.108003049224017	25.54138274162482	32.614185874644306	21.736428334506858
38	21.929274296321726	28.110004829877738	27.937816778878886	22.022904094921643
39	20.24294866945597	30.665766641256464	28.505705656776087	20.58557903251148
40	19.457657101956976	27.325993470936353	31.06879957171205	22.147549855394626
41	19.11566684318027	30.388892445023767	27.18581063387781	23.309630077918147
42	23.402910728729626	26.080699692168036	29.255966062834965	21.260423516267377
43	19.995111930961844	25.55011143633581	30.079605695764254	24.37517093693809
44	19.056253527847446	27.043940249175137	28.315885642461026	25.583920580516388
45	19.11653971265137	24.883821073396682	29.229954552596205	26.76968466135574
46	22.144640290490962	25.36721618649147	28.779379331614752	23.708764191402818
47	17.29986557810145	28.9781608058331	28.94161667064307	24.780356945422383
48	18.868528400263024	28.285102445780257	28.205438558717926	24.64093059523879
49	20.440158978626336	26.304677998452114	31.330311265253396	21.924851757668158
50	19.64532403824332	24.920888930269367	32.90298928698203	22.53079774450529
51	19.15221097837029	24.176447654017817	29.607092355409172	27.06424901220272
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	834.0
1	1047.0
2	1260.0
3	5351.5
4	9443.0
5	6153.0
6	2863.0
7	3073.5
8	3284.0
9	3656.0
10	4028.0
11	4033.5
12	4039.0
13	3997.5
14	3956.0
15	4263.5
16	4571.0
17	4997.5
18	5424.0
19	5224.5
20	5025.0
21	6089.5
22	7154.0
23	7734.0
24	8314.0
25	12140.5
26	19259.0
27	22551.0
28	27078.0
29	31605.0
30	37516.0
31	43427.0
32	51901.5
33	60376.0
34	65567.0
35	70758.0
36	75426.0
37	80094.0
38	96870.5
39	113647.0
40	134909.5
41	156172.0
42	178049.0
43	199926.0
44	210985.0
45	222044.0
46	199669.0
47	177294.0
48	148452.5
49	119611.0
50	102054.5
51	84498.0
52	79046.0
53	73594.0
54	62961.0
55	52328.0
56	47881.0
57	43434.0
58	38524.5
59	33615.0
60	29490.0
61	25365.0
62	19540.5
63	13716.0
64	10315.0
65	6914.0
66	5607.5
67	4301.0
68	3649.5
69	2998.0
70	2374.5
71	1751.0
72	1383.5
73	1016.0
74	810.5
75	454.0
76	303.0
77	228.0
78	153.0
79	166.5
80	180.0
81	96.5
82	13.0
83	10.0
84	7.0
85	4.5
86	2.0
87	3.0
88	4.0
89	2.0
90	0.0
91	3.0
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1718470.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.659740817882668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.22749583807051	10.527651606594699
2	12.395517286498979	3.882211760203165
3	5.776713090758394	2.7138548934163906
4	3.324519535434433	2.0824445707556363
5	2.0921934652819396	1.6381603703591485
6	1.428536603494205	1.34223077757466
7	0.971894444921664	1.0653730576869238
8	0.7435996832920354	0.931566265009033
9	0.5490027503539071	0.7737516700962235
>10	3.1894442255569957	9.836616921581466
>50	0.7059887350140909	8.023399034921676
>100	1.5652585809052644	50.54073563894461
>500	0.02088503229227858	2.0672161078132385
>1k	0.007458940104385209	2.839998443065379
>5k	0.0011188410156577813	1.121912685927571
>10k+	3.729470052192604E-4	0.6128761960501786
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	10494	0.6106594819810646	TruSeq Adapter, Index 15 (95% over 23bp)
ATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG	7770	0.45214638602943313	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6390	0.3718423946882983	No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	5050	0.2938660552700949	TruSeq Adapter, Index 21 (95% over 21bp)
ATGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT	4927	0.2867085256070807	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	4923	0.28647576041478756	TruSeq Adapter, Index 15 (95% over 24bp)
ATATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	4134	0.24056282623496483	No Hit
ATGTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	3672	0.21367844652510665	No Hit
ATTGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	3582	0.20844122969851087	No Hit
ATGCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	3142	0.18283705854626497	No Hit
ATTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	2810	0.163517547585934	No Hit
ATGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	2467	0.14355793234679684	No Hit
ATAGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	2442	0.14210314989496473	No Hit
ATCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT	2236	0.13011574249186778	TruSeq Adapter, Index 15 (95% over 21bp)
GATTGGGGGCGATATCGCTGGGGGATGCATTCTTCATGAATACTTTTGTAT	2111	0.122841830232707	No Hit
ATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	2101	0.12225991725197413	No Hit
ATTCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	1908	0.11102899672382992	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.313418331422719	0.0
2	0.0	0.0	0.0	1.3324061519840322	0.0
3	0.0	0.0	0.0	1.928285044254482	0.0
4	0.0	0.0	0.0	2.5588459501766105	0.0
5	0.0	0.0	0.0	4.6607156365837055	0.0
6	0.0	0.0	0.0	5.1402701240056565	0.0
7	0.0	0.0	0.0	5.892916373285539	0.0
8	0.0	0.0	0.0	8.179368857181098	0.0
9	0.0	0.0	0.0	8.460432826875069	0.0
10	0.0	0.0	0.0	9.07260528260604	0.0
11	0.0	0.0	0.0	9.51090213969403	0.0
12	0.0	0.0	0.0	10.708828201830698	0.0
13	0.0	0.0	0.0	11.789149650561255	0.0
14	0.0	0.0	0.0	12.285812379616752	0.0
15	0.0	0.0	0.0	12.51368950287174	0.0
16	0.0	0.0	0.0	12.645550984305807	0.0
17	5.819129807328612E-5	0.0	0.0	12.829028147130877	0.0
18	5.819129807328612E-5	0.0	0.0	13.271863925468585	0.0
19	1.1638259614657224E-4	0.0	0.0	13.917903716678207	0.0
20	1.1638259614657224E-4	0.0	0.0	14.627372022787712	0.0
21	1.1638259614657224E-4	0.0	0.0	15.103085884536826	0.0
22	1.1638259614657224E-4	0.0	0.0	15.59404586638114	0.0
23	1.7457389421985837E-4	0.0	0.0	16.31579253638411	0.0
24	2.3276519229314447E-4	0.0	0.0	16.981675560236724	0.0
25	3.4914778843971674E-4	0.0	0.0	17.678050824279737	0.0
26	4.0733908651300287E-4	0.0	0.0	18.19321838612254	0.0
27	4.0733908651300287E-4	0.0	0.0	18.63128247801824	0.0
28	4.0733908651300287E-4	0.0	0.0	19.030707547993273	0.0
29	4.0733908651300287E-4	0.0	0.0	19.407554394315873	0.0
30	4.0733908651300287E-4	0.0	0.0	19.82187643659767	0.0
31	4.6553038458628895E-4	0.0	0.0	20.25796202435888	0.0
32	4.6553038458628895E-4	0.0	0.0	20.76963810831728	0.0
33	5.237216826595751E-4	0.0	0.0	21.203919765838215	0.0
34	5.237216826595751E-4	0.0	0.0	21.627145076725228	0.0
35	5.237216826595751E-4	0.0	0.0	22.047693587900866	0.0
36	5.237216826595751E-4	0.0	0.0	22.47010422061485	0.0
37	5.237216826595751E-4	0.0	0.0	23.001798111110464	0.0
38	5.237216826595751E-4	0.0	0.0	23.448183558630642	0.0
39	5.237216826595751E-4	0.0	0.0	23.905159822400158	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGC	90	0.0	45.0	32
TCGCCTC	25	3.8916158E-5	45.0	21
CGGAATA	20	7.0342637E-4	45.0	44
AACCGTA	25	3.8916158E-5	45.0	38
AACCGGT	35	1.2123928E-7	45.0	21
CGGAACG	25	3.8916158E-5	45.0	27
TGCCCCG	40	6.8175723E-9	45.0	2
GTCGATA	55	1.8189894E-12	45.0	10
CGTATCA	25	3.8916158E-5	45.0	20
CGACGAA	45	3.8562575E-10	45.0	21
TAGACGT	40	6.8175723E-9	45.0	28
TCCGAAC	50	2.1827873E-11	45.0	35
TAGCGTC	40	6.8175723E-9	45.0	34
AACCACG	40	6.8175723E-9	45.0	16
TAGCGCG	25	3.8916158E-5	45.0	3
CCGGATA	25	3.8916158E-5	45.0	23
CCGTGTT	35	1.2123928E-7	45.0	37
TGAACGT	25	3.8916158E-5	45.0	14
GCGCCCG	20	7.0342637E-4	45.0	35
TTCGTAA	45	3.8562575E-10	45.0	2
>>END_MODULE