FastQCFastQC Report
Thu 26 May 2016
SRR1545589_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545589_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1441990
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCTT136190.9444586994361959TruSeq Adapter, Index 15 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT73330.5085333462784069TruSeq Adapter, Index 21 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC69780.48391459025374656TruSeq Adapter, Index 15 (95% over 23bp)
GTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC67200.4660226492555427TruSeq Adapter, Index 21 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC62820.4356479587237082TruSeq Adapter, Index 15 (95% over 23bp)
ATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC59710.4140805414739353TruSeq Adapter, Index 15 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC49190.34112580531071646TruSeq Adapter, Index 21 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC38650.2680323719304572TruSeq Adapter, Index 21 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC33760.23412090236409408TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT31650.2194883459663382TruSeq Adapter, Index 21 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC30260.20984888938203453TruSeq Adapter, Index 15 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC27270.1891136554345037TruSeq Adapter, Index 15 (95% over 23bp)
CTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC25380.17600676842419158TruSeq Adapter, Index 15 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT20460.1418872530322679TruSeq Adapter, Index 21 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC14700.10194245452464995TruSeq Adapter, Index 15 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTC351.0198164E-746.00000431
AACGTGC351.0198164E-746.00000412
CGTGGAC351.0198164E-746.00000420
GAAGCGT351.0198164E-746.00000440
ATAGGCG351.0198164E-746.00000412
TCCGAAC351.0198164E-746.00000433
CGCGAAG351.0198164E-746.0000041
CGTTCGA351.0198164E-746.00000433
CCGTAGC351.0198164E-746.00000414
TCGTCGG351.0198164E-746.0000042
TCATCCG351.0198164E-746.00000411
AACTCGC351.0198164E-746.00000426
GCTCGTC351.0198164E-746.00000435
CCGCAAT351.0198164E-746.00000434
ATCTCGC206.3119916E-446.014
GGTACGT206.3119916E-446.024
TCGCACT253.4175915E-546.037
AAATCCG453.110472E-1046.013
TGCCCGC301.8617357E-646.034
ACACGGT501.6370905E-1146.036
Produced by FastQC (version 0.11.5)