##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545589_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1441990 Sequences flagged as poor quality 0 Sequence length 52 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.606739991262074 33.0 31.0 34.0 31.0 34.0 2 32.99742023176305 34.0 33.0 34.0 31.0 34.0 3 33.058473359732034 34.0 33.0 34.0 31.0 34.0 4 36.41866448449712 37.0 37.0 37.0 35.0 37.0 5 36.40154855442825 37.0 37.0 37.0 35.0 37.0 6 36.41037108440419 37.0 37.0 37.0 35.0 37.0 7 36.517287914617995 37.0 37.0 37.0 35.0 37.0 8 36.571570537937156 37.0 37.0 37.0 35.0 37.0 9 38.3934860852017 39.0 39.0 39.0 37.0 39.0 10 38.139017607611706 39.0 39.0 39.0 37.0 39.0 11 37.89722328171485 39.0 38.0 39.0 35.0 39.0 12 37.60835234641017 39.0 37.0 39.0 35.0 39.0 13 37.552541973245305 39.0 37.0 39.0 35.0 39.0 14 38.90118724817786 41.0 38.0 41.0 35.0 41.0 15 38.958480988078975 41.0 38.0 41.0 35.0 41.0 16 39.04586508921698 41.0 38.0 41.0 35.0 41.0 17 39.01192241277679 41.0 38.0 41.0 35.0 41.0 18 39.015896088045 41.0 38.0 41.0 35.0 41.0 19 39.00598686537354 41.0 38.0 41.0 35.0 41.0 20 38.90927745684783 41.0 38.0 41.0 35.0 41.0 21 38.88861919985575 41.0 38.0 41.0 35.0 41.0 22 38.95791510343345 41.0 38.0 41.0 35.0 41.0 23 38.95262796551987 41.0 38.0 41.0 35.0 41.0 24 38.944214592334205 41.0 38.0 41.0 35.0 41.0 25 38.86197477097622 41.0 38.0 41.0 35.0 41.0 26 38.75751357499012 40.0 38.0 41.0 35.0 41.0 27 38.72399600552015 40.0 38.0 41.0 35.0 41.0 28 38.73487194779437 40.0 38.0 41.0 35.0 41.0 29 38.750893556820785 40.0 38.0 41.0 35.0 41.0 30 38.68584109459844 40.0 37.0 41.0 35.0 41.0 31 38.60043342880325 40.0 37.0 41.0 35.0 41.0 32 38.57713992468741 40.0 37.0 41.0 35.0 41.0 33 38.435254058627315 40.0 37.0 41.0 35.0 41.0 34 38.43613201201118 40.0 37.0 41.0 35.0 41.0 35 38.37365030270668 40.0 37.0 41.0 35.0 41.0 36 38.23006192830741 40.0 36.0 41.0 35.0 41.0 37 38.22606259405405 40.0 36.0 41.0 34.0 41.0 38 38.17539095278053 40.0 36.0 41.0 34.0 41.0 39 38.14201762841628 40.0 36.0 41.0 34.0 41.0 40 38.00411792037393 40.0 36.0 41.0 34.0 41.0 41 37.91384406271888 40.0 36.0 41.0 34.0 41.0 42 37.8979098329392 40.0 35.0 41.0 34.0 41.0 43 37.844149404642195 40.0 35.0 41.0 34.0 41.0 44 37.77375571259163 40.0 35.0 41.0 34.0 41.0 45 37.683567847211144 40.0 35.0 41.0 34.0 41.0 46 37.704923057718844 40.0 35.0 41.0 34.0 41.0 47 37.683283517916216 40.0 35.0 41.0 34.0 41.0 48 37.597227442631365 40.0 35.0 41.0 34.0 41.0 49 37.58869825726947 40.0 35.0 41.0 34.0 41.0 50 37.51076359752842 39.0 35.0 41.0 34.0 41.0 51 37.39268372180112 39.0 35.0 41.0 33.0 41.0 52 37.051308261499734 39.0 35.0 41.0 33.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 0.0 11 4.0 12 6.0 13 6.0 14 5.0 15 16.0 16 24.0 17 47.0 18 68.0 19 168.0 20 284.0 21 529.0 22 924.0 23 1392.0 24 2032.0 25 2871.0 26 3691.0 27 4926.0 28 6728.0 29 8907.0 30 11645.0 31 15456.0 32 20789.0 33 29226.0 34 62846.0 35 157929.0 36 70621.0 37 98281.0 38 183086.0 39 758553.0 40 927.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.894215632563334 14.198850200070737 46.74560849936546 13.16132566800047 2 22.551196610240016 12.581709997988892 52.31506459822883 12.552028793542258 3 24.003287124043858 13.279773091352922 47.25760927606987 15.459330508533345 4 20.06109612410627 14.361750081484615 51.546682015825354 14.030471778583763 5 21.81471438775581 17.105319731759582 45.993592188572734 15.086373691911872 6 23.80189876490128 16.88451376223136 46.7557333962094 12.557854076657952 7 84.52728521002226 1.8338546037073764 7.602757300674762 6.0361028855956 8 86.47951788847357 1.9702633166665513 8.03868265383255 3.5115361410273307 9 78.33251270813251 4.234842127892704 10.027392700365468 7.405252463609317 10 40.2509726142345 28.56788188544997 13.999056858924124 17.18208864139141 11 27.941594601904313 22.14099959084321 33.16312873182199 16.754277075430483 12 26.313844062718882 25.72250847786739 28.179182934694413 19.78446452471931 13 20.153191076221056 28.152553069022666 35.64268822946068 16.051567625295597 14 14.83248843611953 36.92945166055243 30.404163690455547 17.83389621287249 15 13.08733070270945 25.960374205091576 40.01643562021928 20.935859471979697 16 17.00316923140937 24.79101796822447 36.73118398879326 21.474628811572895 17 17.522243566182844 24.755650177879183 28.872738368504635 28.849367887433335 18 18.290487451369287 24.348296451431704 33.70661377679457 23.654602320404443 19 20.977121894049198 27.449150132802586 30.81928446105729 20.75444351209093 20 23.75564324301833 23.305501425113906 28.04221943286708 24.896635899000685 21 21.059299995145597 28.388199640774207 30.757772245299897 19.7947281187803 22 25.7816628409351 22.366174522708203 26.533956546161903 25.3182060901948 23 22.105839846323484 27.358858244509328 24.647327651370677 25.88797425779652 24 22.052996206631114 21.947100881420813 33.12637396930631 22.873528942641766 25 24.977427027926684 25.48263164099612 27.832578589310607 21.707362741766588 26 19.0214911337804 30.68911712286493 26.436244356756983 23.853147386597687 27 17.315654061401258 26.726884375065012 29.347082850782595 26.610378712751125 28 18.227935006484095 28.158655746572446 30.79522049390079 22.81818875304267 29 16.33402450779825 22.484483248843613 31.029757487916005 30.151734755442135 30 17.848528769270246 28.02252442804735 30.757425502257295 23.37152130042511 31 19.981830664567717 33.09024334426729 24.4861614851698 22.441764505995188 32 23.34787342491973 26.16599282935388 25.07472312568048 25.41141062004591 33 20.242373386777995 27.199425793521453 27.73264724443304 24.825553575267513 34 17.0232109792717 30.667757751440718 27.818431473172488 24.49059979611509 35 20.715400245494074 24.47887988127518 31.963952593291218 22.84176727993953 36 20.711863466459548 26.75940887246097 28.159834672917288 24.36889298816219 37 20.96706634581377 30.703472284828603 26.253025333046693 22.07643603631093 38 17.64235535613978 26.522236631321995 32.92505495877226 22.910353053765974 39 19.88287020020943 30.670947787432645 25.88547770788979 23.560704304468132 40 24.36813015346847 24.471112837120923 30.48481612216451 20.675940887246096 41 19.798126200597785 24.909187997142837 30.623652036421888 24.66903376583749 42 19.166291028370516 27.427652064161336 26.5745254821462 26.831531425321952 43 19.07398803043017 23.47048176478339 29.47322796968079 27.982302235105657 44 25.1889402839132 23.502104730268584 25.731385099757976 25.577569886060235 45 17.562119016081944 26.914472361112075 28.56822862849257 26.955179994313415 46 18.80186409059702 27.210521570884683 27.445752050985096 26.541862287533203 47 20.84570628090347 24.977912468186325 31.490440294315498 22.685940956594706 48 18.210875248788135 23.353629359426904 35.12409933494685 23.31139605683812 49 19.2257227858723 22.620267824326103 28.16156838813029 29.9924410016713 50 17.86683680191957 23.937336597341176 32.868605191436835 25.327221409302425 51 21.021574352110626 23.998502070055963 30.07558998328699 24.904333594546426 52 18.652417839236055 22.364926247754838 31.396680975596226 27.58597493741288 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 414.0 1 317.0 2 220.0 3 186.5 4 153.0 5 226.0 6 299.0 7 522.0 8 745.0 9 835.5 10 926.0 11 1073.5 12 1221.0 13 1321.0 14 1623.0 15 1825.0 16 2152.0 17 2479.0 18 2466.5 19 2454.0 20 2779.0 21 3104.0 22 3590.5 23 4077.0 24 5224.5 25 6372.0 26 7832.5 27 9293.0 28 12755.0 29 16217.0 30 18697.0 31 21177.0 32 27322.5 33 33468.0 34 38790.0 35 44112.0 36 46414.0 37 48716.0 38 52673.5 39 68566.5 40 80502.0 41 99168.0 42 117834.0 43 134904.0 44 151974.0 45 175651.5 46 199329.0 47 195529.0 48 191729.0 49 157663.5 50 123598.0 51 101814.5 52 80031.0 53 70572.0 54 61113.0 55 55897.5 56 50682.0 57 44614.0 58 38546.0 59 34499.5 60 30453.0 61 25528.0 62 20603.0 63 18449.0 64 12955.5 65 9616.0 66 7572.0 67 5528.0 68 4272.5 69 3017.0 70 2538.0 71 2059.0 72 1822.5 73 1586.0 74 1229.5 75 873.0 76 708.0 77 543.0 78 458.0 79 373.0 80 267.0 81 161.0 82 102.5 83 44.0 84 96.0 85 148.0 86 77.0 87 6.0 88 6.0 89 6.0 90 6.0 91 4.5 92 3.0 93 3.0 94 3.0 95 4.0 96 5.0 97 2.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 1441990.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.15646966344701 #Duplication Level Percentage of deduplicated Percentage of total 1 62.42802846009729 10.086165479643682 2 13.317722548167273 4.303347606713375 3 6.508749170014677 3.1547522553698406 4 3.8529354695765643 2.4899934011773084 5 2.526429019024972 2.0409086901364573 6 1.776582491076511 1.7221980673013257 7 1.2621877077949197 1.4274748187395037 8 0.9413210779900223 1.2166740352087224 9 0.7056905457974146 1.0261321105461563 >10 4.258804811891591 13.330948113049516 >50 0.893528706872624 10.479412896064936 >100 1.5061113553237848 41.89787085102241 >500 0.014176176476236415 1.450151015306785 >1k 0.00515497326408597 2.1186613330743236 >5k 0.0021479055267024875 2.3100807118536912 >10k+ 4.2958110534049745E-4 0.9452286147919547 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCTT 13619 0.9444586994361959 TruSeq Adapter, Index 15 (96% over 25bp) GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT 7333 0.5085333462784069 TruSeq Adapter, Index 21 (95% over 22bp) TTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC 6978 0.48391459025374656 TruSeq Adapter, Index 15 (95% over 23bp) GTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC 6720 0.4660226492555427 TruSeq Adapter, Index 21 (95% over 21bp) TGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC 6282 0.4356479587237082 TruSeq Adapter, Index 15 (95% over 23bp) ATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC 5971 0.4140805414739353 TruSeq Adapter, Index 15 (95% over 23bp) GCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC 4919 0.34112580531071646 TruSeq Adapter, Index 21 (95% over 21bp) GGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC 3865 0.2680323719304572 TruSeq Adapter, Index 21 (95% over 21bp) AGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC 3376 0.23412090236409408 TruSeq Adapter, Index 15 (95% over 23bp) CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT 3165 0.2194883459663382 TruSeq Adapter, Index 21 (95% over 22bp) TCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC 3026 0.20984888938203453 TruSeq Adapter, Index 15 (95% over 23bp) ACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC 2727 0.1891136554345037 TruSeq Adapter, Index 15 (95% over 23bp) CTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC 2538 0.17600676842419158 TruSeq Adapter, Index 15 (95% over 23bp) TCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT 2046 0.1418872530322679 TruSeq Adapter, Index 21 (95% over 22bp) CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC 1470 0.10194245452464995 TruSeq Adapter, Index 15 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.9782314717855186 0.0 2 0.0 0.0 0.0 1.9268510877329246 0.0 3 0.0 0.0 0.0 5.649969833355294 0.0 4 0.0 0.0 0.0 6.454760435231867 0.0 5 0.0 0.0 0.0 7.689997850193136 0.0 6 0.0 0.0 0.0 11.49272879839666 0.0 7 0.0 0.0 0.0 11.959791676780005 0.0 8 0.0 0.0 0.0 12.98559629401036 0.0 9 0.0 0.0 0.0 13.652036421889195 0.0 10 0.0 0.0 0.0 15.692064438727037 0.0 11 0.0 0.0 0.0 17.461632882336215 0.0 12 6.934860852017005E-5 0.0 0.0 18.239654921324004 0.0 13 6.934860852017005E-5 0.0 0.0 18.5796711488984 0.0 14 6.934860852017005E-5 0.0 0.0 18.7522798355051 0.0 15 6.934860852017005E-5 0.0 0.0 19.010603402242733 0.0 16 6.934860852017005E-5 0.0 0.0 19.66539296389018 0.0 17 6.934860852017005E-5 0.0 0.0 20.6924458560739 0.0 18 6.934860852017005E-5 0.0 0.0 21.775185680899313 0.0 19 6.934860852017005E-5 0.0 0.0 22.47546792973599 0.0 20 1.386972170403401E-4 0.0 0.0 23.212227546654276 0.0 21 1.386972170403401E-4 0.0 0.0 24.292956261832607 0.0 22 1.386972170403401E-4 0.0 0.0 25.26106283677418 0.0 23 1.386972170403401E-4 0.0 0.0 26.32202719852426 0.0 24 2.0804582556051014E-4 0.0 0.0 27.079105957738957 0.0 25 2.0804582556051014E-4 0.0 0.0 27.71967905463977 0.0 26 2.0804582556051014E-4 0.0 0.0 28.297907752480945 0.0 27 2.0804582556051014E-4 0.0 0.0 28.832030735303295 0.0 28 2.0804582556051014E-4 0.0 0.0 29.394239904576313 0.0 29 2.0804582556051014E-4 0.0 0.0 29.973231437111213 0.0 30 2.0804582556051014E-4 0.0 0.0 30.666440127878833 0.0 31 2.0804582556051014E-4 0.0 0.0 31.229204086020015 0.0 32 2.0804582556051014E-4 0.0 0.0 31.772689130992585 0.0 33 2.0804582556051014E-4 0.0 0.0 32.30383012364857 0.0 34 2.0804582556051014E-4 0.0 0.0 32.8392707300328 0.0 35 2.0804582556051014E-4 0.0 0.0 33.51971927683271 0.0 36 2.0804582556051014E-4 0.0 0.0 34.08726828896178 0.0 37 2.0804582556051014E-4 0.0 0.0 34.651280522056325 0.0 38 2.0804582556051014E-4 0.0 0.0 35.20697092212845 0.0 39 2.0804582556051014E-4 0.0 0.0 35.74005367582299 0.0 40 2.0804582556051014E-4 0.0 0.0 36.2881157289579 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTCGTC 35 1.0198164E-7 46.000004 31 AACGTGC 35 1.0198164E-7 46.000004 12 CGTGGAC 35 1.0198164E-7 46.000004 20 GAAGCGT 35 1.0198164E-7 46.000004 40 ATAGGCG 35 1.0198164E-7 46.000004 12 TCCGAAC 35 1.0198164E-7 46.000004 33 CGCGAAG 35 1.0198164E-7 46.000004 1 CGTTCGA 35 1.0198164E-7 46.000004 33 CCGTAGC 35 1.0198164E-7 46.000004 14 TCGTCGG 35 1.0198164E-7 46.000004 2 TCATCCG 35 1.0198164E-7 46.000004 11 AACTCGC 35 1.0198164E-7 46.000004 26 GCTCGTC 35 1.0198164E-7 46.000004 35 CCGCAAT 35 1.0198164E-7 46.000004 34 ATCTCGC 20 6.3119916E-4 46.0 14 GGTACGT 20 6.3119916E-4 46.0 24 TCGCACT 25 3.4175915E-5 46.0 37 AAATCCG 45 3.110472E-10 46.0 13 TGCCCGC 30 1.8617357E-6 46.0 34 ACACGGT 50 1.6370905E-11 46.0 36 >>END_MODULE