SRR1545588_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1545588_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1299302
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	3915	0.3013156294687455	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3160	0.24320750679980482	No Hit
ATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG	2013	0.15492933898354655	Illumina Single End Adapter 2 (95% over 21bp)
ATGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT	1799	0.13845895719393952	No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	1723	0.13260966272660243	Illumina Single End Adapter 2 (95% over 23bp)
ATGCCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC	1686	0.1297619798938199	No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC	1476	0.11359945570775694	No Hit
ATGTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC	1418	0.10913552045636811	No Hit
ATTGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC	1378	0.10605694442092754	TruSeq Adapter, Index 22 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAA	25	3.891087E-5	45.0	29
CCTTACG	25	3.891087E-5	45.0	6
TCGTTAC	20	7.0336263E-4	45.0	2
GTCGCAT	20	7.0336263E-4	45.0	42
AAATCGG	20	7.0336263E-4	45.0	19
CCGATAG	20	7.0336263E-4	45.0	37
CGTTGTA	35	1.2120836E-7	45.0	34
TAGCGAT	25	3.891087E-5	45.0	2
CACGCAT	25	3.891087E-5	45.0	25
TCGATTA	35	1.2120836E-7	45.0	2
CGACACT	20	7.0336263E-4	45.0	27
GCGCAAC	25	3.891087E-5	45.0	32
CGTTATC	25	3.891087E-5	45.0	44
CGTTACA	20	7.0336263E-4	45.0	34
TGCATCG	25	3.891087E-5	45.0	18
GTTACGC	20	7.0336263E-4	45.0	22
AAACGAC	20	7.0336263E-4	45.0	33
CTAACGC	25	3.891087E-5	45.0	23
CCCGTAA	20	7.0336263E-4	45.0	38
CTAACCG	35	1.2120836E-7	45.0	43