##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545588_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1299302
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.925689331656535	31.0	31.0	34.0	31.0	34.0
2	32.59933179507151	34.0	31.0	34.0	31.0	34.0
3	32.576070074547715	34.0	31.0	34.0	31.0	34.0
4	36.04283299802509	37.0	35.0	37.0	35.0	37.0
5	35.83093230057369	37.0	35.0	37.0	35.0	37.0
6	35.83441801828982	37.0	35.0	37.0	35.0	37.0
7	35.83196901105363	37.0	35.0	37.0	35.0	37.0
8	35.79984791834385	37.0	35.0	37.0	35.0	37.0
9	37.96296165171761	39.0	38.0	39.0	35.0	39.0
10	37.890739797214195	39.0	38.0	39.0	35.0	39.0
11	37.70744753721613	39.0	38.0	39.0	35.0	39.0
12	37.25440659677273	39.0	37.0	39.0	34.0	39.0
13	37.02769563965883	39.0	37.0	39.0	33.0	39.0
14	37.7878122253333	40.0	36.0	41.0	33.0	41.0
15	37.69166444752644	40.0	36.0	41.0	33.0	41.0
16	37.72084473047836	40.0	36.0	41.0	33.0	41.0
17	37.8059758239424	40.0	36.0	41.0	33.0	41.0
18	37.861931252318556	40.0	36.0	41.0	33.0	41.0
19	37.81516383412016	40.0	36.0	41.0	33.0	41.0
20	37.74889594566929	40.0	36.0	41.0	33.0	41.0
21	37.731035586799685	40.0	36.0	41.0	33.0	41.0
22	37.66077247629881	40.0	35.0	41.0	33.0	41.0
23	37.589015486776745	40.0	35.0	41.0	33.0	41.0
24	37.53369193613186	40.0	35.0	41.0	33.0	41.0
25	37.48468331457968	39.0	35.0	41.0	32.0	41.0
26	37.38730256707063	39.0	35.0	41.0	32.0	41.0
27	37.291025489070286	39.0	35.0	41.0	32.0	41.0
28	37.19391488660835	39.0	35.0	41.0	32.0	41.0
29	37.10184237382841	39.0	35.0	41.0	32.0	41.0
30	37.02228889049659	39.0	35.0	41.0	31.0	41.0
31	36.934600269991115	39.0	35.0	41.0	31.0	41.0
32	36.86156798034637	39.0	35.0	41.0	31.0	41.0
33	36.7452740009636	39.0	35.0	41.0	31.0	41.0
34	36.63438831003108	39.0	35.0	41.0	31.0	41.0
35	36.5659654183554	39.0	35.0	41.0	31.0	41.0
36	36.467748067808714	39.0	35.0	41.0	30.0	41.0
37	36.28311970581127	38.0	35.0	41.0	30.0	41.0
38	36.205929029586656	38.0	35.0	41.0	30.0	41.0
39	36.152277145729016	38.0	35.0	40.0	30.0	41.0
40	36.093858086880495	38.0	35.0	40.0	30.0	41.0
41	36.003899786192896	38.0	35.0	40.0	30.0	41.0
42	35.83966852971827	38.0	35.0	40.0	29.0	41.0
43	35.72525709958116	38.0	35.0	40.0	29.0	41.0
44	35.654504495490656	38.0	35.0	40.0	29.0	41.0
45	35.62448991843313	37.0	35.0	40.0	29.0	41.0
46	35.55015385183737	37.0	34.0	40.0	29.0	41.0
47	35.46443475035057	37.0	34.0	40.0	29.0	41.0
48	35.3653923414264	37.0	34.0	40.0	28.0	41.0
49	35.31962392115151	37.0	34.0	40.0	28.0	41.0
50	35.19239407004684	36.0	34.0	40.0	28.0	41.0
51	35.015903154155076	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	16.0
11	8.0
12	14.0
13	21.0
14	31.0
15	40.0
16	76.0
17	173.0
18	300.0
19	578.0
20	1015.0
21	1750.0
22	2808.0
23	4220.0
24	6070.0
25	8706.0
26	11744.0
27	14512.0
28	17184.0
29	20476.0
30	24737.0
31	31298.0
32	39196.0
33	51171.0
34	82574.0
35	153864.0
36	97021.0
37	136992.0
38	206992.0
39	385514.0
40	192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.1562346552225735	91.25545870013283	3.135760585298876	3.4525460593457105
2	3.208953730541475	2.665123273880899	91.45902954047635	2.6668934551012775
3	28.212763468385333	15.636857328011503	43.5691625195682	12.581216684034965
4	26.800466712126976	15.354859763165146	44.110761008603085	13.733912516104802
5	24.244632887504213	13.54565759153761	47.45193957986673	14.757769941091448
6	23.046989845316947	14.670338381684935	47.76156736463116	14.521104408366952
7	22.74767529027124	17.174606057714065	44.25214461303069	15.825574038984008
8	26.023972871587976	19.305442460644255	43.28508691589792	11.385497751869849
9	83.13071172060076	4.11467080016809	8.858140755574917	3.89647672365624
10	83.48066885142946	4.0995088131935455	8.865144516055544	3.554677819321451
11	77.19891141551388	7.115820648317326	9.751081734654452	5.934186201514351
12	43.74902832443882	22.699803432920138	15.60214638321191	17.94902185942914
13	34.43487349361426	23.003658887618123	23.053223961788714	19.5082436569789
14	28.51538749266914	22.780539089449565	28.66277432036586	20.041299097515434
15	22.50623796469181	24.722196995001934	32.36091378293884	20.41065125736742
16	17.689343970839726	32.118629848949666	30.55055714529801	19.641469034912593
17	17.45491040574093	23.537407007762628	39.01617945635426	19.991503130142185
18	22.35115469690649	19.70619609605773	36.62882070527098	21.313828501764796
19	22.85557938031343	19.912460690432248	29.386316653095278	27.845643276159045
20	23.368547112218714	21.643236137556933	31.071760068098104	23.91645668212625
21	26.980101623794926	23.8121699189257	26.871120032140333	22.336608425139037
22	28.56926257328935	21.656166156905783	26.159353252746477	23.61521801705839
23	27.10693895645508	23.856578378236932	27.19006050941198	21.84642215589601
24	28.8391767271966	21.026520393257304	25.52147229820319	24.612830581342905
25	27.663853361266277	23.63053393283471	24.022898448551608	24.682714257347406
26	27.077923377321056	20.36100921879594	29.654768483385695	22.906298920497314
27	27.413488165184074	21.64061934792681	26.745898951898788	24.199993534990323
28	23.84149335566327	26.80723957940494	24.75136650293773	24.599900561994055
29	23.257025695334878	24.975640767119575	27.73181292724863	24.035520610296913
30	24.165051696988073	24.189757269672484	28.45427775836565	23.19091327497379
31	23.132189437097765	24.759601693832533	27.88320190379142	24.225006965278283
32	24.01258521883288	24.81724803009616	27.823862350708307	23.346304400362655
33	28.163583216219166	24.186062978429955	24.465289824844415	23.185063980506456
34	27.325286961768704	24.666628697562228	26.027667162830504	21.98041717783856
35	27.854032395855622	24.44435550780342	23.842570857275675	23.85904123906528
36	25.358538661527497	24.359925560031463	26.25417339463804	24.027362383803
37	25.363233489981546	23.24517317759843	28.40325036057822	22.988342971841806
38	27.862190622349537	25.63437907430297	23.768454139222445	22.734976164125044
39	25.485837780592963	27.512387420322604	24.16612919860048	22.835645600483954
40	25.34522382017422	25.560339320650627	25.16612765931246	23.928309199862696
41	24.53686671766841	25.620987268548806	24.803240509134906	25.03890550464788
42	26.582195671214237	23.921998118990043	26.659698822906453	22.836107386889267
43	23.275420187146636	23.389635358061483	27.945850926112637	25.389093528679247
44	23.698801356420603	24.61198397293316	25.660316077401557	26.02889859324468
45	23.24078620674793	23.297201112597378	27.056142451870315	26.40587022878438
46	24.561726219154593	24.609598076505694	25.880126406332018	24.9485492980077
47	23.155278757363572	25.130108319697808	25.941620962639938	25.772991960298686
48	23.229318511015915	25.20868897300243	25.229623290043424	26.332369225938233
49	24.048450629645764	23.919843115765236	28.199525591432938	23.832180663156063
50	24.00065573669555	22.159975125105632	29.45712390191041	24.38224523628841
51	22.44351197796971	23.70295743406845	27.469518249029097	26.38401233893275
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	609.0
1	1295.0
2	1981.0
3	4006.0
4	6031.0
5	4255.5
6	2480.0
7	2490.0
8	2500.0
9	2561.0
10	2622.0
11	2804.0
12	2986.0
13	2989.5
14	2993.0
15	2997.0
16	3001.0
17	3020.0
18	3039.0
19	3751.0
20	4463.0
21	4330.0
22	4197.0
23	4792.5
24	5388.0
25	7028.5
26	10945.5
27	13222.0
28	13934.0
29	14646.0
30	17386.0
31	20126.0
32	21955.5
33	23785.0
34	26233.0
35	28681.0
36	34346.5
37	40012.0
38	45835.0
39	51658.0
40	61339.0
41	71020.0
42	76937.0
43	82854.0
44	84243.5
45	85633.0
46	97991.5
47	110350.0
48	113573.5
49	116797.0
50	108956.0
51	101115.0
52	92142.0
53	83169.0
54	81187.0
55	79205.0
56	79976.0
57	80747.0
58	77339.0
59	73931.0
60	65749.5
61	57568.0
62	48583.5
63	39599.0
64	33381.5
65	27164.0
66	22246.5
67	17329.0
68	14567.5
69	11806.0
70	9546.5
71	7287.0
72	6025.5
73	4764.0
74	3912.0
75	2390.5
76	1721.0
77	1165.0
78	609.0
79	376.0
80	143.0
81	179.5
82	216.0
83	139.5
84	63.0
85	45.0
86	27.0
87	15.5
88	4.0
89	8.5
90	13.0
91	12.5
92	12.0
93	7.5
94	3.0
95	3.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1299302.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.801860851920587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.2819544799026	12.312732021281017
2	10.95452542447228	3.681128237616191
3	4.305185818794567	2.1700539920714434
4	2.1734396200870623	1.4607132026701586
5	1.2131361320638379	1.0191472242686879
6	0.8012674134087088	0.8077670151162872
7	0.5515756456839604	0.6487248073663108
8	0.39758800033969616	0.5344174606480744
9	0.3058261110168297	0.4624602985969911
>10	2.837074591693245	11.20869743483854
>50	1.4474956971915547	17.85902353360973
>100	1.7180685202740926	45.243224954543706
>500	0.006890649145484329	0.7966913771157081
>1k	0.005971895926086418	1.7952184402571454
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	3915	0.3013156294687455	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3160	0.24320750679980482	No Hit
ATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG	2013	0.15492933898354655	Illumina Single End Adapter 2 (95% over 21bp)
ATGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT	1799	0.13845895719393952	No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	1723	0.13260966272660243	Illumina Single End Adapter 2 (95% over 23bp)
ATGCCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC	1686	0.1297619798938199	No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC	1476	0.11359945570775694	No Hit
ATGTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC	1418	0.10913552045636811	No Hit
ATTGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC	1378	0.10605694442092754	TruSeq Adapter, Index 22 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.146617183687857	0.0
2	0.0	0.0	0.0	0.5995526829020504	0.0
3	0.0	0.0	0.0	0.8346019632079378	0.0
4	0.0	0.0	0.0	1.1306840134164344	0.0
5	0.0	0.0	0.0	2.1916382796301397	0.0
6	0.0	0.0	0.0	2.448468485386769	0.0
7	0.0	0.0	0.0	2.869463758233267	0.0
8	0.0	0.0	0.0	4.174703032859181	0.0
9	0.0	0.0	0.0	4.334712022301205	0.0
10	0.0	0.0	0.0	4.653883392775506	0.0
11	0.0	0.0	0.0	4.907865915699352	0.0
12	0.0	0.0	0.0	5.628329672393331	0.0
13	0.0	0.0	0.0	6.225958245273231	0.0
14	0.0	0.0	0.0	6.475938619351005	0.0
15	0.0	0.0	0.0	6.619400262602536	0.0
16	0.0	0.0	0.0	6.735924365543961	0.0
17	0.0	0.0	0.0	6.865609381036895	0.0
18	7.696440088601418E-5	0.0	0.0	7.160690894033873	0.0
19	1.5392880177202836E-4	0.0	0.0	7.525579118634467	0.0
20	1.5392880177202836E-4	0.0	0.0	7.955887083988172	0.0
21	2.3089320265804256E-4	0.0	0.0	8.218412655410367	0.0
22	3.078576035440567E-4	0.0	0.0	8.550744938436175	0.0
23	3.078576035440567E-4	0.0	0.0	8.97912879376773	0.0
24	3.848220044300709E-4	0.0	0.0	9.365105264211092	0.0
25	3.848220044300709E-4	0.0	0.0	9.767398187642288	0.0
26	3.848220044300709E-4	0.0	0.0	10.061479163427748	0.0
27	4.617864053160851E-4	0.0	0.0	10.338551006617399	0.0
28	4.617864053160851E-4	0.0	0.0	10.583990481042898	0.0
29	4.617864053160851E-4	0.0	0.0	10.822580123789542	0.0
30	4.617864053160851E-4	0.0	0.0	11.084874802009079	0.0
31	5.387508062020993E-4	0.0	0.0	11.359175926766833	0.0
32	5.387508062020993E-4	0.0	0.0	11.66433977627988	0.0
33	5.387508062020993E-4	0.0	0.0	11.956650570844962	0.0
34	5.387508062020993E-4	0.0	0.0	12.265200853996992	0.0
35	6.157152070881134E-4	0.0	0.0	12.565669875055992	0.0
36	6.157152070881134E-4	0.0	0.0	12.836122779769445	0.0
37	6.157152070881134E-4	0.0	0.0	13.17107185242538	0.0
38	6.157152070881134E-4	0.0	0.0	13.48423999963057	0.0
39	6.157152070881134E-4	0.0	0.0	13.804642800519048	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAA	25	3.891087E-5	45.0	29
CCTTACG	25	3.891087E-5	45.0	6
TCGTTAC	20	7.0336263E-4	45.0	2
GTCGCAT	20	7.0336263E-4	45.0	42
AAATCGG	20	7.0336263E-4	45.0	19
CCGATAG	20	7.0336263E-4	45.0	37
CGTTGTA	35	1.2120836E-7	45.0	34
TAGCGAT	25	3.891087E-5	45.0	2
CACGCAT	25	3.891087E-5	45.0	25
TCGATTA	35	1.2120836E-7	45.0	2
CGACACT	20	7.0336263E-4	45.0	27
GCGCAAC	25	3.891087E-5	45.0	32
CGTTATC	25	3.891087E-5	45.0	44
CGTTACA	20	7.0336263E-4	45.0	34
TGCATCG	25	3.891087E-5	45.0	18
GTTACGC	20	7.0336263E-4	45.0	22
AAACGAC	20	7.0336263E-4	45.0	33
CTAACGC	25	3.891087E-5	45.0	23
CCCGTAA	20	7.0336263E-4	45.0	38
CTAACCG	35	1.2120836E-7	45.0	43
>>END_MODULE