FastQCFastQC Report
Thu 26 May 2016
SRR1545587_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545587_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1093698
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCTT36770.33619884099632624Illumina Single End Adapter 2 (95% over 24bp)
GTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC29200.2669841217593888TruSeq Adapter, Index 22 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT28930.2645154329622985TruSeq Adapter, Index 22 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC27300.24961186726134635TruSeq Adapter, Index 22 (95% over 24bp)
TGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC25090.22940519229257073Illumina Single End Adapter 2 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC22840.20883278565015204Illumina Single End Adapter 2 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC18820.1720767524490307Illumina Single End Adapter 2 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC17750.16229343017908052TruSeq Adapter, Index 22 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC13040.11922852560761746Illumina Single End Adapter 2 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC11830.10816514247991675Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAG253.417047E-546.012
CGGACAA206.311319E-446.027
AACCGGA206.311319E-446.013
GATACGA206.311319E-446.09
ACCGGTA253.417047E-546.023
AAATCGG301.8613191E-646.029
GCCCAAT206.311319E-446.026
CGAACGT253.417047E-546.036
CGTATTG253.417047E-546.01
CCGATGT206.311319E-446.017
CTATATG206.311319E-446.01
CGCATAC301.8613191E-646.012
TACGGTA253.417047E-546.022
TCGATCT206.311319E-446.012
ATCACGC253.417047E-546.011
TCGATAG206.311319E-446.024
AACGGGA253.417047E-546.04
AACGGCA206.311319E-446.032
ACCTATC253.417047E-546.045
AATGCCG253.417047E-546.038
Produced by FastQC (version 0.11.5)