##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545587_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1093698
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.580453653567986	33.0	31.0	34.0	31.0	34.0
2	32.99369021430047	34.0	31.0	34.0	31.0	34.0
3	33.072625167093655	34.0	33.0	34.0	31.0	34.0
4	36.44576382145711	37.0	37.0	37.0	35.0	37.0
5	36.43128724748514	37.0	37.0	37.0	35.0	37.0
6	36.442824253130205	37.0	37.0	37.0	35.0	37.0
7	36.50871721444128	37.0	37.0	37.0	35.0	37.0
8	36.53957673873409	37.0	37.0	37.0	35.0	37.0
9	38.35057483875805	39.0	39.0	39.0	37.0	39.0
10	38.11788537603616	39.0	39.0	39.0	35.0	39.0
11	37.957861310892035	39.0	38.0	39.0	35.0	39.0
12	37.62270846248233	39.0	37.0	39.0	35.0	39.0
13	37.55474637422762	39.0	37.0	39.0	35.0	39.0
14	38.89442972374458	41.0	38.0	41.0	35.0	41.0
15	38.93019828142687	41.0	38.0	41.0	35.0	41.0
16	38.984538693496745	41.0	38.0	41.0	35.0	41.0
17	38.934501114567276	41.0	38.0	41.0	35.0	41.0
18	38.912186910829135	41.0	38.0	41.0	35.0	41.0
19	38.89379609362	40.0	38.0	41.0	35.0	41.0
20	38.8118987142703	40.0	38.0	41.0	35.0	41.0
21	38.767276707098304	40.0	38.0	41.0	35.0	41.0
22	38.84351438879837	40.0	38.0	41.0	35.0	41.0
23	38.85956360896701	41.0	38.0	41.0	35.0	41.0
24	38.80848369476766	41.0	38.0	41.0	35.0	41.0
25	38.70502551892753	40.0	38.0	41.0	35.0	41.0
26	38.585624185104116	40.0	37.0	41.0	35.0	41.0
27	38.472478691558365	40.0	37.0	41.0	35.0	41.0
28	38.41445719019327	40.0	37.0	41.0	35.0	41.0
29	38.386141329690645	40.0	36.0	41.0	35.0	41.0
30	38.34554419958709	40.0	36.0	41.0	35.0	41.0
31	38.2663102611507	40.0	36.0	41.0	35.0	41.0
32	38.165864799972205	40.0	36.0	41.0	34.0	41.0
33	38.12020228618869	40.0	36.0	41.0	34.0	41.0
34	38.01888089765182	40.0	36.0	41.0	34.0	41.0
35	37.87643938271808	40.0	35.0	41.0	34.0	41.0
36	37.813075455930246	40.0	35.0	41.0	34.0	41.0
37	37.732566942611214	40.0	35.0	41.0	34.0	41.0
38	37.664785891534954	40.0	35.0	41.0	33.0	41.0
39	37.5535623179342	40.0	35.0	41.0	33.0	41.0
40	37.40265228609726	39.0	35.0	41.0	33.0	41.0
41	37.26739282690468	39.0	35.0	41.0	33.0	41.0
42	37.192758878593544	39.0	35.0	41.0	33.0	41.0
43	37.11128574798528	39.0	35.0	41.0	33.0	41.0
44	36.97772236942922	39.0	35.0	41.0	33.0	41.0
45	36.90859451146478	39.0	35.0	41.0	33.0	41.0
46	36.85916587577192	39.0	35.0	41.0	33.0	41.0
47	36.79841784477982	38.0	35.0	41.0	33.0	41.0
48	36.68521474849547	38.0	35.0	40.0	33.0	41.0
49	36.609925226159326	38.0	35.0	40.0	32.0	41.0
50	36.49650634818752	38.0	35.0	40.0	32.0	41.0
51	36.36413982653347	37.0	35.0	40.0	32.0	41.0
52	36.01681359936655	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	1.0
12	7.0
13	8.0
14	19.0
15	32.0
16	41.0
17	71.0
18	149.0
19	287.0
20	547.0
21	907.0
22	1168.0
23	1562.0
24	2275.0
25	3094.0
26	3754.0
27	4779.0
28	6456.0
29	8005.0
30	10200.0
31	13291.0
32	17697.0
33	24947.0
34	57078.0
35	120258.0
36	67182.0
37	99162.0
38	173898.0
39	476166.0
40	652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.638434010119795	13.27605975324084	47.59513137996046	11.490374856678901
2	24.9965712655596	12.945072588593925	50.16339062520001	11.894965520646467
3	25.6515052601358	13.354874928910906	46.07734493434202	14.916274876611277
4	23.32472035241904	14.823653330261187	47.224096597049645	14.62752972027013
5	22.910894963691987	16.91134115633383	44.26532735727778	15.912436522696394
6	25.811878599028248	18.931551488619345	43.55279062410282	11.70377928824959
7	86.94667083600774	2.912229884300785	6.1143021199636465	4.026797159727822
8	87.7050154613065	2.865599095911303	6.279429970613461	3.149955472168734
9	80.44451027614569	5.011255392256364	8.250540825712399	6.293693505885536
10	44.38775603502978	22.05243129273346	13.653860572114057	19.905952100122704
11	34.21959261148873	21.87194271179064	23.56235450736858	20.346110169352052
12	28.866743836049807	22.758019124109214	27.500644602074797	20.874592437766186
13	23.26976916845418	23.14276884478165	33.33068177869942	20.256780208064747
14	17.736248946235616	32.43628497080547	29.80676567023072	20.020700412728193
15	17.463687416453173	23.217103807449586	37.920705715837464	21.39850306025978
16	22.222405088059045	20.474847718474386	35.17634666973881	22.126400523727757
17	22.754544673209605	20.690446540086935	28.086638176169288	28.468370610534173
18	23.144597503149864	21.9217736523245	29.133727957809196	25.799900886716443
19	26.089468939323286	24.764880250306756	26.169655608769517	22.97599520160044
20	28.727765800065463	20.94499578494246	24.94317444120772	25.384063973784354
21	27.132718538389938	24.376381779979482	26.721453271378387	21.76944641025219
22	28.892345053204814	20.722448061530695	24.880725757933178	25.504481127331314
23	28.065608604934816	24.04521174949575	21.96712437985623	25.922055265713205
24	27.614844317169823	19.560975698958945	27.842603716931	24.981576266940234
25	28.70490757046278	21.435624825134543	24.534926460503723	25.324541143898955
26	24.329933857426823	26.306439254711993	24.0346055309601	25.329021356901084
27	23.659639132557615	23.93786950328153	26.69832074302047	25.70417062114039
28	25.326095503511937	23.497162836541715	27.053263332291	24.12347832765535
29	23.683137392589177	24.872131063602566	25.75153287287716	25.6931986709311
30	24.129147168596816	24.244718377468004	27.25276996026325	24.37336449367193
31	29.415524212351123	23.953321666492943	22.98788148099384	23.643272640162092
32	27.687259188551135	24.89910377453374	24.597192277941442	22.816444758973685
33	28.823953230233574	23.461961162953575	22.957708617918293	24.75637698889456
34	25.650316632196457	23.458486712053965	25.677837940638092	25.213358715111482
35	25.476502654297622	22.07903827199099	28.80767817075646	23.63678090295493
36	28.833096522074648	24.380861992981608	22.803552717477768	23.982488767465973
37	26.159049390233868	26.39220333218128	22.947102399382647	24.501644878202207
38	25.42365442745621	24.017964739809344	25.926626911633743	24.631753921100703
39	25.260355235174607	24.710660529689182	24.386896565596718	25.64208766953949
40	28.473307988128347	22.490943569431415	26.81279475687073	22.222953685569507
41	23.826321342820414	22.35973733151199	27.71688345411622	26.097057871551378
42	23.97499126815629	24.25294734012497	24.74193058778566	27.030130803933076
43	23.853111187914763	22.16050500229497	26.797982624088185	27.188401185702087
44	26.920228436003356	22.920312554288294	24.204579326285682	25.954879683422664
45	23.570583470025547	23.03926678114068	25.896911213150247	27.493238535683524
46	23.618037154680728	24.043931688637997	24.808219453633455	27.529811703047823
47	24.98733654080011	22.430049245769858	28.256977703168516	24.325636510261518
48	24.216465605679083	20.52038131184294	30.559990052098478	24.703163030379503
49	23.02317458750039	21.98659959147772	26.428319334953525	28.561906486068366
50	21.679659284372836	23.045849951266252	29.45292027598112	25.82157048837979
51	24.241518225323627	23.66622230268319	26.342738123321062	25.749521348672122
52	24.040914402330444	21.63366852641223	27.20239042221893	27.1230266490384
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	127.0
1	105.5
2	84.0
3	83.0
4	82.0
5	113.5
6	145.0
7	208.0
8	271.0
9	345.0
10	419.0
11	665.0
12	911.0
13	924.0
14	855.5
15	774.0
16	943.5
17	1113.0
18	1197.5
19	1282.0
20	1512.5
21	1743.0
22	2173.5
23	2604.0
24	2694.5
25	2785.0
26	4009.0
27	5233.0
28	6919.5
29	8606.0
30	8899.0
31	9192.0
32	11652.5
33	14113.0
34	16271.5
35	18430.0
36	19363.5
37	20297.0
38	22397.0
39	32347.0
40	40197.0
41	45321.0
42	50445.0
43	54676.5
44	58908.0
45	62355.0
46	65802.0
47	75145.0
48	84488.0
49	95203.0
50	105918.0
51	106724.0
52	107530.0
53	96744.0
54	85958.0
55	78982.0
56	72006.0
57	69280.0
58	66554.0
59	63965.0
60	61376.0
61	58135.0
62	54894.0
63	48288.5
64	33658.5
65	25634.0
66	22884.0
67	20134.0
68	17202.0
69	14270.0
70	11413.5
71	8557.0
72	7498.5
73	6440.0
74	5306.0
75	4172.0
76	3224.0
77	2276.0
78	1849.0
79	1422.0
80	1107.0
81	792.0
82	517.0
83	242.0
84	169.5
85	97.0
86	111.5
87	126.0
88	95.0
89	37.5
90	11.0
91	8.0
92	5.0
93	10.0
94	15.0
95	12.5
96	10.0
97	8.0
98	6.0
99	13.5
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1093698.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.467701270656413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.10113413128117	12.070379684687795
2	12.093794735711505	4.225015873440914
3	5.109499910484272	2.677536542363549
4	2.658683226249269	1.8576433747770904
5	1.626271265449163	1.420361032495918
6	1.0328238578976006	1.0824635168977308
7	0.7071172209782505	0.8646198665569371
8	0.5343152765002894	0.7466607707404191
9	0.4158163788774262	0.6537020660709268
>10	3.506475744946644	14.251623553522554
>50	1.708864436113975	21.97673917017867
>100	1.4963034799056738	35.612598489755925
>500	0.0036648440725471367	0.4429020461204119
>1k	0.005235491532210195	2.117754012391158
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCTT	3677	0.33619884099632624	Illumina Single End Adapter 2 (95% over 24bp)
GTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2920	0.2669841217593888	TruSeq Adapter, Index 22 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	2893	0.2645154329622985	TruSeq Adapter, Index 22 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2730	0.24961186726134635	TruSeq Adapter, Index 22 (95% over 24bp)
TGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2509	0.22940519229257073	Illumina Single End Adapter 2 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2284	0.20883278565015204	Illumina Single End Adapter 2 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	1882	0.1720767524490307	Illumina Single End Adapter 2 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	1775	0.16229343017908052	TruSeq Adapter, Index 22 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	1304	0.11922852560761746	Illumina Single End Adapter 2 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	1183	0.10816514247991675	Illumina Single End Adapter 2 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.35713697931238786	0.0
2	0.0	0.0	0.0	0.8152159005502433	0.0
3	0.0	0.0	0.0	2.766211513598818	0.0
4	0.0	0.0	0.0	3.2199016547529573	0.0
5	0.0	0.0	0.0	3.9783377129701254	0.0
6	0.0	0.0	0.0	6.3663826760220825	0.0
7	0.0	0.0	0.0	6.664088258367483	0.0
8	0.0	0.0	0.0	7.246424515725548	0.0
9	0.0	0.0	0.0	7.650832313856293	0.0
10	0.0	0.0	0.0	8.966826308542212	0.0
11	0.0	0.0	0.0	10.011630267221847	0.0
12	0.0	0.0	0.0	10.454348458166697	0.0
13	0.0	0.0	0.0	10.667387158063743	0.0
14	0.0	0.0	0.0	10.796947603451775	0.0
15	0.0	0.0	0.0	10.992248317177136	0.0
16	0.0	0.0	0.0	11.469070986689196	0.0
17	0.0	0.0	0.0	12.083408765491022	0.0
18	0.0	0.0	0.0	12.800059979994478	0.0
19	0.0	0.0	0.0	13.227966038156785	0.0
20	0.0	0.0	0.0	13.786529736728054	0.0
21	0.0	0.0	0.0	14.500346530760776	0.0
22	0.0	0.0	0.0	15.106546779824047	0.0
23	0.0	0.0	0.0	15.721341723217927	0.0
24	0.0	0.0	0.0	16.190575460501893	0.0
25	0.0	0.0	0.0	16.617567189480095	0.0
26	0.0	0.0	0.0	16.98201880226534	0.0
27	0.0	0.0	0.0	17.33933864741455	0.0
28	0.0	0.0	0.0	17.70735614401782	0.0
29	0.0	0.0	0.0	18.089545742974753	0.0
30	0.0	0.0	0.0	18.530160976796154	0.0
31	0.0	0.0	0.0	18.943894932604795	0.0
32	0.0	0.0	0.0	19.361926235578743	0.0
33	0.0	0.0	0.0	19.774197264692813	0.0
34	0.0	0.0	0.0	20.144226285501116	0.0
35	0.0	0.0	0.0	20.586944476445964	0.0
36	0.0	0.0	0.0	21.002507090622824	0.0
37	0.0	0.0	0.0	21.422001320291344	0.0
38	0.0	0.0	0.0	21.842135580388735	0.0
39	0.0	0.0	0.0	22.25148075611366	0.0
40	0.0	0.0	0.0	22.701056415939316	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAG	25	3.417047E-5	46.0	12
CGGACAA	20	6.311319E-4	46.0	27
AACCGGA	20	6.311319E-4	46.0	13
GATACGA	20	6.311319E-4	46.0	9
ACCGGTA	25	3.417047E-5	46.0	23
AAATCGG	30	1.8613191E-6	46.0	29
GCCCAAT	20	6.311319E-4	46.0	26
CGAACGT	25	3.417047E-5	46.0	36
CGTATTG	25	3.417047E-5	46.0	1
CCGATGT	20	6.311319E-4	46.0	17
CTATATG	20	6.311319E-4	46.0	1
CGCATAC	30	1.8613191E-6	46.0	12
TACGGTA	25	3.417047E-5	46.0	22
TCGATCT	20	6.311319E-4	46.0	12
ATCACGC	25	3.417047E-5	46.0	11
TCGATAG	20	6.311319E-4	46.0	24
AACGGGA	25	3.417047E-5	46.0	4
AACGGCA	20	6.311319E-4	46.0	32
ACCTATC	25	3.417047E-5	46.0	45
AATGCCG	25	3.417047E-5	46.0	38
>>END_MODULE