FastQCFastQC Report
Thu 26 May 2016
SRR1545586_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545586_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1477682
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC65290.4418406666657644TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36490.24694081676571822No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC33660.22778919957067895No Hit
ATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG31530.2133747315051547No Hit
ATGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT26090.17656031541292375No Hit
ATTGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC21420.14495676336315932No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT20810.14082867626458195TruSeq Adapter, Index 21 (95% over 22bp)
ATGTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC20700.14008426711565816No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC20210.13676826272499767TruSeq Adapter, Index 15 (95% over 22bp)
ATATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC19800.13399364680628173No Hit
ATGCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC19500.13196344003648958No Hit
ATAGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC16570.1121350872515196No Hit
ATGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC16250.10996953336374132No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15820.10705957032703924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGT351.2122473E-745.00000433
AGCGTTC650.045.0000047
GTACCTA207.0339406E-445.00000435
CGACGTG207.0339406E-445.0000042
CGACGTC207.0339406E-445.00000441
CGAAACG207.0339406E-445.00000442
CGACGGT351.2122473E-745.00000430
CCGATCA207.0339406E-445.00000418
CGTGCAT207.0339406E-445.00000421
ACCGAAT800.045.0000042
AGGCGTA207.0339406E-445.00000414
TACGGGT207.0339406E-445.0000046
GCGCATT207.0339406E-445.00000431
ACGGGTA207.0339406E-445.0000047
AACGGTA351.2122473E-745.00000421
CTAACCG351.2122473E-745.00000419
CCGTAGA351.2122473E-745.00000425
CGCTCAT406.8175723E-945.00000427
TATTACG351.2122473E-745.00000415
GTATCGC207.0339406E-445.00000411