##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545586_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1477682
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.932729775418526	31.0	31.0	34.0	31.0	34.0
2	32.59523767630654	34.0	31.0	34.0	31.0	34.0
3	32.56006096034194	34.0	31.0	34.0	31.0	34.0
4	36.026318247092405	37.0	35.0	37.0	35.0	37.0
5	35.79987236766774	37.0	35.0	37.0	35.0	37.0
6	35.80239117753346	37.0	35.0	37.0	35.0	37.0
7	35.78744953244338	37.0	35.0	37.0	35.0	37.0
8	35.75934673360033	37.0	35.0	37.0	33.0	37.0
9	37.92129632762665	39.0	38.0	39.0	35.0	39.0
10	37.8848527626377	39.0	38.0	39.0	35.0	39.0
11	37.69520505765111	39.0	38.0	39.0	35.0	39.0
12	37.247635147480985	39.0	37.0	39.0	34.0	39.0
13	36.983736013567196	39.0	37.0	39.0	33.0	39.0
14	37.68201683447454	40.0	36.0	41.0	33.0	41.0
15	37.59003628656233	40.0	36.0	41.0	33.0	41.0
16	37.6319898327245	40.0	36.0	41.0	33.0	41.0
17	37.70445061928074	40.0	35.0	41.0	33.0	41.0
18	37.75327370841629	40.0	35.0	41.0	33.0	41.0
19	37.719162174270245	40.0	35.0	41.0	33.0	41.0
20	37.696482057709304	40.0	36.0	41.0	33.0	41.0
21	37.665531555503826	40.0	36.0	41.0	33.0	41.0
22	37.571731265590294	40.0	35.0	41.0	33.0	41.0
23	37.51768783811402	39.0	35.0	41.0	32.0	41.0
24	37.4795152136928	39.0	35.0	41.0	32.0	41.0
25	37.403152369725014	39.0	35.0	41.0	32.0	41.0
26	37.336155546321876	39.0	35.0	41.0	32.0	41.0
27	37.267879015918176	39.0	35.0	41.0	32.0	41.0
28	37.17432302755261	39.0	35.0	41.0	32.0	41.0
29	37.12300955144612	39.0	35.0	41.0	32.0	41.0
30	37.05465519644957	39.0	35.0	41.0	31.0	41.0
31	36.99355409350591	39.0	35.0	41.0	31.0	41.0
32	36.83562363214819	39.0	35.0	41.0	31.0	41.0
33	36.71450894035388	39.0	35.0	41.0	31.0	41.0
34	36.664559763196685	39.0	35.0	41.0	31.0	41.0
35	36.48139586189721	39.0	35.0	41.0	30.0	41.0
36	36.40122502676489	38.0	35.0	41.0	30.0	41.0
37	36.27459967706178	38.0	35.0	41.0	30.0	41.0
38	36.14878844027335	38.0	35.0	41.0	30.0	41.0
39	36.128937078478316	38.0	35.0	40.0	30.0	41.0
40	36.06758219968843	38.0	35.0	40.0	30.0	41.0
41	36.03817262442122	38.0	35.0	40.0	30.0	41.0
42	35.898118133671524	38.0	35.0	40.0	29.0	41.0
43	35.77950059620405	38.0	35.0	40.0	29.0	41.0
44	35.6833229341631	38.0	35.0	40.0	29.0	41.0
45	35.644702987516936	37.0	35.0	40.0	29.0	41.0
46	35.58135918282824	37.0	34.0	40.0	29.0	41.0
47	35.49822424581202	37.0	34.0	40.0	29.0	41.0
48	35.45531988614601	37.0	34.0	40.0	29.0	41.0
49	35.42083208701195	37.0	34.0	40.0	29.0	41.0
50	35.30244463964507	37.0	34.0	40.0	28.0	41.0
51	35.11521694112806	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	11.0
11	18.0
12	10.0
13	19.0
14	16.0
15	36.0
16	78.0
17	149.0
18	324.0
19	639.0
20	1251.0
21	2067.0
22	3248.0
23	4875.0
24	7070.0
25	10009.0
26	13616.0
27	16742.0
28	19936.0
29	24144.0
30	29031.0
31	36209.0
32	44686.0
33	57965.0
34	92943.0
35	181105.0
36	107408.0
37	150363.0
38	226878.0
39	446588.0
40	245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.3268199788587802	91.09876143852331	3.346119124412424	3.2282994582054867
2	3.4883012718568676	2.248183303308831	91.13902720612418	3.124488218710115
3	26.74770349777557	16.0158274919773	43.43898078206272	13.797488228184413
4	26.728145839226574	15.112114785183822	44.227039376537036	13.93269999905257
5	23.42127737903013	14.093966090133058	47.55001414377383	14.934742387062979
6	21.84488949584552	15.037132481819498	47.72549168224287	15.392486340092118
7	21.66034370047141	16.886177134187193	46.205002158786534	15.248477006554861
8	25.69754520932109	18.797684481505495	43.53230261991416	11.972467689259258
9	82.36291705522568	4.209363042928045	8.950775606659619	4.47694429518665
10	83.66786629328908	4.094182645521838	8.423260214308627	3.8146908468804517
11	76.39749282998642	6.843353306056378	10.076795954745338	6.68235790921186
12	43.92535065054592	21.921428291066682	15.412314692877088	18.740906365510305
13	32.7251059429566	22.0736261252421	24.482669478277465	20.71859845352383
14	27.073551684327207	22.20004033344116	29.66091486530932	21.065493116922315
15	21.442773208308687	27.76652892841626	31.54115702837282	19.24954083490223
16	17.793747233843277	31.74877950736356	31.464347538915682	18.993125719877487
17	16.80943531828905	22.85660920279194	40.36775165428015	19.96620382463886
18	20.508066011496386	20.187970077459156	36.50338841509878	22.800575495945676
19	19.882965347077384	20.308158318230852	29.753153926216875	30.055722408474892
20	22.220207054021095	21.54787024542493	32.65262756127502	23.57929513927895
21	26.580617480621676	23.988043435597103	27.21011692637523	22.221222157405993
22	26.886434293711364	21.433840298521602	27.442507927957436	24.237217479809594
23	25.142689699136888	24.503445260888338	28.323482318929244	22.03038272104553
24	27.911079650425464	21.574262933432227	24.3924606241397	26.12219679200261
25	25.843787770305116	25.191008620257943	23.746178135755866	25.21902547368108
26	26.264040571652085	20.310932934149566	31.07299134725875	22.3520351469396
27	25.68874764665199	23.618951844848894	27.42017565348972	23.272124855009398
28	22.945329238631857	26.49555181696738	25.92127399535218	24.637844949048578
29	21.408327366781215	26.154003364729355	26.406967128245455	26.030702140243978
30	21.115774571254168	25.874579239646962	30.57667346560356	22.43297272349531
31	22.011501798086464	23.91637713662344	29.185372766265	24.886748299025093
32	22.664416295251616	27.83948102501079	27.885566718685073	21.610535961052513
33	25.395653462652994	28.731350858980488	24.217389127024624	21.6556065513419
34	28.484410042214765	24.509400534079727	24.984062876857134	22.022126546848376
35	25.25211784402869	26.4573162561363	24.393002012611646	23.897563887223367
36	24.366541651045353	25.42136941507036	25.45283762000214	24.759251313882146
37	23.651638173842546	25.112845659620948	28.0884520485463	23.147064117990205
38	26.171463142949563	26.401756264202987	26.034221165311617	21.39255942753583
39	25.777264661814925	27.62725674400852	25.97568353678261	20.619795057393944
40	22.88855112263667	26.773351776633948	27.939096503848592	22.399000596880793
41	23.126152988261346	28.651090018014703	23.847079412214537	24.375677581509418
42	25.685702336497297	23.280381029206552	27.073890052122174	23.960026582173974
43	22.60432217486577	23.765668120745868	27.227982745949397	26.402026958438963
44	23.12520555843544	25.033870616276033	25.183226160973742	26.65769766431478
45	21.21667584771284	23.60771803405604	28.346897370340844	26.82870874789028
46	22.38397706678433	24.694893759279736	28.45937082538733	24.461758348548603
47	21.34139821693707	27.38931651058888	26.263769877416117	25.005515395057937
48	21.395943105485483	26.6575623171968	25.873158094908106	26.07333648240961
49	22.88983692025754	24.26381318849387	29.07980201423581	23.76654787701278
50	22.642896103491818	23.427909387811454	30.153172333424916	23.77602217527181
51	21.44629223337633	24.07162028095355	28.629299132018932	25.852788353651192
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	464.0
1	876.5
2	1289.0
3	3709.5
4	6130.0
5	4191.0
6	2252.0
7	2374.0
8	2496.0
9	2524.0
10	2552.0
11	2721.5
12	2891.0
13	2844.0
14	2797.0
15	2804.0
16	2811.0
17	2780.5
18	2750.0
19	2610.5
20	2471.0
21	3439.5
22	4408.0
23	5157.5
24	5907.0
25	7724.0
26	12472.5
27	15404.0
28	16496.5
29	17589.0
30	21111.5
31	24634.0
32	28512.0
33	32390.0
34	39320.5
35	46251.0
36	53056.5
37	59862.0
38	64223.5
39	68585.0
40	81734.0
41	94883.0
42	108269.5
43	121656.0
44	133362.5
45	145069.0
46	141054.0
47	137039.0
48	121114.0
49	105189.0
50	95703.0
51	86217.0
52	85937.0
53	85657.0
54	78779.0
55	71901.0
56	74303.5
57	76706.0
58	74591.5
59	72477.0
60	63882.5
61	55288.0
62	46941.0
63	38594.0
64	32929.0
65	27264.0
66	22290.5
67	17317.0
68	14808.0
69	12299.0
70	9876.0
71	7453.0
72	5914.0
73	4375.0
74	3484.0
75	2014.5
76	1436.0
77	869.5
78	303.0
79	282.0
80	261.0
81	232.0
82	203.0
83	111.0
84	19.0
85	12.0
86	5.0
87	4.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1477682.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.008771110483778
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.58319328655442	11.459589566819686
2	11.594917974046863	3.71240775782701
3	4.871979494332885	2.3398321373923694
4	2.6082296573164334	1.6701820635021725
5	1.5299264440030915	1.2246121128960934
6	0.9470785789093072	0.909693851604081
7	0.6614758432991992	0.741259075934378
8	0.4618844223470315	0.5915361597481316
9	0.3402550580790659	0.49023588095749177
>10	2.642994407158166	9.1740101890643
>50	0.9425652606648145	11.162624055920443
>100	1.7972426786088025	52.521951105248576
>500	0.009340736812991648	0.9808732178628361
>1k	0.008491578920901499	2.5774175331826448
>5k	4.2457894604507496E-4	0.44377529203980715
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	6529	0.4418406666657644	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3649	0.24694081676571822	No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC	3366	0.22778919957067895	No Hit
ATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	3153	0.2133747315051547	No Hit
ATGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT	2609	0.17656031541292375	No Hit
ATTGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	2142	0.14495676336315932	No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	2081	0.14082867626458195	TruSeq Adapter, Index 21 (95% over 22bp)
ATGTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	2070	0.14008426711565816	No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	2021	0.13676826272499767	TruSeq Adapter, Index 15 (95% over 22bp)
ATATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	1980	0.13399364680628173	No Hit
ATGCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	1950	0.13196344003648958	No Hit
ATAGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	1657	0.1121350872515196	No Hit
ATGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	1625	0.10996953336374132	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1582	0.10705957032703924	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.154092693827224	0.0
2	0.0	0.0	0.0	0.8072778852283509	0.0
3	0.0	0.0	0.0	1.130757497215233	0.0
4	0.0	0.0	0.0	1.4923373229152146	0.0
5	0.0	0.0	0.0	2.877885769739362	0.0
6	0.0	0.0	0.0	3.1829581736801287	0.0
7	0.0	0.0	0.0	3.690645213246152	0.0
8	0.0	0.0	0.0	5.216954662775888	0.0
9	0.0	0.0	0.0	5.399334904262216	0.0
10	0.0	0.0	0.0	5.774584788878798	0.0
11	0.0	0.0	0.0	6.053264504812267	0.0
12	0.0	0.0	0.0	6.807757014026022	0.0
13	0.0	0.0	0.0	7.482936112099897	0.0
14	0.0	0.0	0.0	7.7692629401995825	0.0
15	1.3534711798614317E-4	0.0	0.0	7.919430567605208	0.0
16	1.3534711798614317E-4	0.0	0.0	8.022159030156692	0.0
17	1.3534711798614317E-4	0.0	0.0	8.151347854274466	0.0
18	1.3534711798614317E-4	0.0	0.0	8.467654069008082	0.0
19	1.3534711798614317E-4	0.0	0.0	8.90780289669902	0.0
20	1.3534711798614317E-4	0.0	0.0	9.392616273325384	0.0
21	3.3836779496535793E-4	0.0	0.0	9.693019201695629	0.0
22	4.7371491295150104E-4	0.0	0.0	10.059133155848146	0.0
23	5.413884719445727E-4	0.0	0.0	10.532509701004681	0.0
24	6.090620309376442E-4	0.0	0.0	10.948363721017106	0.0
25	6.767355899307159E-4	0.0	0.0	11.431485258668644	0.0
26	6.767355899307159E-4	0.0	0.0	11.778785963421088	0.0
27	6.767355899307159E-4	0.0	0.0	12.097460752719462	0.0
28	6.767355899307159E-4	0.0	0.0	12.38385525437814	0.0
29	7.444091489237874E-4	0.0	0.0	12.661046152013762	0.0
30	7.444091489237874E-4	0.0	0.0	12.984390416882658	0.0
31	7.444091489237874E-4	0.0	0.0	13.299343160436413	0.0
32	8.12082707916859E-4	0.0	0.0	13.63459797168809	0.0
33	8.12082707916859E-4	0.0	0.0	13.939399681392885	0.0
34	8.797562669099306E-4	0.0	0.0	14.251374788350944	0.0
35	8.797562669099306E-4	0.0	0.0	14.597660389718492	0.0
36	9.474298259030021E-4	0.0	0.0	14.916064484780893	0.0
37	9.474298259030021E-4	0.0	0.0	15.301939118159387	0.0
38	9.474298259030021E-4	0.0	0.0	15.629208449449882	0.0
39	0.0010151033848960736	0.0	0.0	15.94686813536336	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	35	1.2122473E-7	45.000004	33
AGCGTTC	65	0.0	45.000004	7
GTACCTA	20	7.0339406E-4	45.000004	35
CGACGTG	20	7.0339406E-4	45.000004	2
CGACGTC	20	7.0339406E-4	45.000004	41
CGAAACG	20	7.0339406E-4	45.000004	42
CGACGGT	35	1.2122473E-7	45.000004	30
CCGATCA	20	7.0339406E-4	45.000004	18
CGTGCAT	20	7.0339406E-4	45.000004	21
ACCGAAT	80	0.0	45.000004	2
AGGCGTA	20	7.0339406E-4	45.000004	14
TACGGGT	20	7.0339406E-4	45.000004	6
GCGCATT	20	7.0339406E-4	45.000004	31
ACGGGTA	20	7.0339406E-4	45.000004	7
AACGGTA	35	1.2122473E-7	45.000004	21
CTAACCG	35	1.2122473E-7	45.000004	19
CCGTAGA	35	1.2122473E-7	45.000004	25
CGCTCAT	40	6.8175723E-9	45.000004	27
TATTACG	35	1.2122473E-7	45.000004	15
GTATCGC	20	7.0339406E-4	45.000004	11
>>END_MODULE