FastQCFastQC Report
Thu 26 May 2016
SRR1545585_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545585_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1207203
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCTT55240.4575866693505566TruSeq Adapter, Index 21 (95% over 23bp)
GTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC40120.3323384716572109TruSeq Adapter, Index 15 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC38270.3170137913838849TruSeq Adapter, Index 21 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT37890.31386601921963414TruSeq Adapter, Index 15 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC35780.2963876000970839TruSeq Adapter, Index 21 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC29340.24304114552399225TruSeq Adapter, Index 15 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC28910.23947919281181376TruSeq Adapter, Index 21 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC27110.22456869308641547TruSeq Adapter, Index 15 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC20590.1705595496366394TruSeq Adapter, Index 21 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC17090.1415669112816983TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT12980.10752127024203882TruSeq Adapter, Index 15 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC12180.10089438147519514TruSeq Adapter, Index 21 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG206.311582E-446.024
AACCGTC206.311582E-446.014
AGCGTAC301.8614828E-646.040
GGTACGC206.311582E-446.034
GTCGCAA301.8614828E-646.027
ACACGTC206.311582E-446.038
TACCCCC253.4172594E-546.037
CGTATTG206.311582E-446.01
CGTATAG650.046.01
GGTCGAC206.311582E-446.013
CCGATTT206.311582E-446.011
CCGATTG600.046.044
CACGGGT206.311582E-446.032
CAACGGC301.8614828E-646.024
CACGGCA253.4172594E-546.036
CGACGGT301.8614828E-646.028
CCCTATG253.4172594E-546.011
TCCGATA453.092282E-1046.020
CTATCGT206.311582E-446.021
GCGAAAC206.311582E-446.024
Produced by FastQC (version 0.11.5)