##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545585_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1207203
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55791279511399	33.0	31.0	34.0	31.0	34.0
2	32.97554760881144	34.0	31.0	34.0	31.0	34.0
3	33.05072800514909	34.0	33.0	34.0	31.0	34.0
4	36.42650987447844	37.0	37.0	37.0	35.0	37.0
5	36.40049685098529	37.0	37.0	37.0	35.0	37.0
6	36.411007096569506	37.0	37.0	37.0	35.0	37.0
7	36.48060102567671	37.0	37.0	37.0	35.0	37.0
8	36.5329675290734	37.0	37.0	37.0	35.0	37.0
9	38.34590122787965	39.0	39.0	39.0	37.0	39.0
10	38.1189816460032	39.0	39.0	39.0	37.0	39.0
11	37.92861681092575	39.0	38.0	39.0	35.0	39.0
12	37.54097943759252	39.0	37.0	39.0	35.0	39.0
13	37.4875584305208	39.0	37.0	39.0	35.0	39.0
14	38.83801150262218	41.0	38.0	41.0	35.0	41.0
15	38.86047831226397	41.0	38.0	41.0	35.0	41.0
16	38.90349758905503	41.0	38.0	41.0	35.0	41.0
17	38.85589167687622	41.0	38.0	41.0	35.0	41.0
18	38.8600715869659	41.0	38.0	41.0	35.0	41.0
19	38.81990104398349	41.0	38.0	41.0	35.0	41.0
20	38.72944152723279	40.0	38.0	41.0	35.0	41.0
21	38.6803304829428	40.0	38.0	41.0	35.0	41.0
22	38.78644436768298	41.0	38.0	41.0	35.0	41.0
23	38.79316568961475	41.0	38.0	41.0	35.0	41.0
24	38.760464478633665	41.0	38.0	41.0	35.0	41.0
25	38.69762169245769	40.0	37.0	41.0	35.0	41.0
26	38.57802705924356	40.0	37.0	41.0	35.0	41.0
27	38.49522739754623	40.0	37.0	41.0	35.0	41.0
28	38.443840016964835	40.0	37.0	41.0	35.0	41.0
29	38.4362721099931	40.0	36.0	41.0	35.0	41.0
30	38.348211526975994	40.0	36.0	41.0	35.0	41.0
31	38.24943857826728	40.0	36.0	41.0	35.0	41.0
32	38.23135628390585	40.0	36.0	41.0	35.0	41.0
33	38.032483352012875	40.0	35.0	41.0	34.0	41.0
34	37.94554602664175	40.0	35.0	41.0	34.0	41.0
35	37.865629061557996	40.0	35.0	41.0	34.0	41.0
36	37.72842098636269	40.0	35.0	41.0	34.0	41.0
37	37.70887000777831	40.0	35.0	41.0	34.0	41.0
38	37.63427940454091	40.0	35.0	41.0	33.0	41.0
39	37.59249107233829	40.0	35.0	41.0	33.0	41.0
40	37.4431475070887	40.0	35.0	41.0	33.0	41.0
41	37.30795069263413	39.0	35.0	41.0	33.0	41.0
42	37.23505657292104	39.0	35.0	41.0	33.0	41.0
43	37.14750046181131	39.0	35.0	41.0	33.0	41.0
44	37.02583658257973	39.0	35.0	41.0	33.0	41.0
45	36.960994132718355	39.0	35.0	41.0	33.0	41.0
46	36.9428977562183	39.0	35.0	41.0	33.0	41.0
47	36.90950072191669	39.0	35.0	41.0	33.0	41.0
48	36.79465176942072	38.0	35.0	41.0	33.0	41.0
49	36.722933922463746	38.0	35.0	40.0	33.0	41.0
50	36.60560651356897	38.0	35.0	40.0	32.0	41.0
51	36.485886797829366	38.0	35.0	40.0	32.0	41.0
52	36.16421761708677	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	6.0
12	4.0
13	6.0
14	12.0
15	21.0
16	34.0
17	77.0
18	163.0
19	290.0
20	539.0
21	923.0
22	1373.0
23	2004.0
24	2639.0
25	3467.0
26	4563.0
27	5663.0
28	7209.0
29	9183.0
30	11551.0
31	15007.0
32	19402.0
33	27117.0
34	61718.0
35	139131.0
36	69700.0
37	104629.0
38	180474.0
39	539530.0
40	765.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.449569790664867	13.98016737864303	47.242841510499886	12.327421320192213
2	25.10779048759819	12.509412252951657	50.39434129968199	11.988455959768158
3	24.815710365199557	13.932950796179266	45.922682432035046	15.328656406586132
4	21.89060166351475	14.754353658829542	48.18394255150128	15.171102126154427
5	21.92970030723913	16.622390766093194	46.12513388386212	15.322775042805558
6	24.62220521320772	18.528366811547023	44.5910091343378	12.258418840907453
7	86.0012773328098	2.6543174594496532	6.584311006516717	4.76009420122382
8	87.74597147290059	2.496514670689188	6.525828713149322	3.2316851432609095
9	79.8215378855089	4.406715357731881	8.752546174918386	7.019200581840835
10	44.851197354546	20.997462729963395	13.597713060686562	20.553626854804037
11	32.42163911123481	20.90642584552888	25.17289966973243	21.499035373503876
12	27.606707405465357	21.87171503052925	28.993632388256156	21.527945175749235
13	21.808759587244232	26.348012720312987	32.899189282995486	18.94403840944729
14	17.58080455399796	32.45932954109624	30.760443769606272	19.199422135299532
15	16.59132722499861	22.481554469298036	39.26398459911051	21.663133706592845
16	20.3331999671969	21.25085838918558	35.03023103819324	23.385710605424272
17	19.990921162389423	21.090156336589622	28.168501900674535	30.750420600346423
18	21.624117898978053	22.193781824597853	30.730125753497962	25.451974522926136
19	25.920578394851567	25.034646202834153	26.82224944769024	22.222525954624036
20	27.214229918249046	20.685170596825884	26.187062159388276	25.9135373255368
21	24.807509590350588	25.331116639040825	28.091547154869563	21.769826615739028
22	27.625594038450867	21.116332547218654	24.204545548677398	27.05352786565308
23	25.94890834432983	25.35331671640975	22.20587589659734	26.491899042663082
24	26.156661307170374	19.387708612387478	30.239073295874846	24.2165567845673
25	28.271715693218123	22.704963456850255	24.898877819223443	24.12444303070817
26	23.269988560333267	26.408648752529608	24.39109246746405	25.93027021967308
27	22.907911925334844	24.144820713666217	24.969702692919086	27.97756466807985
28	22.120057687066716	25.338903233341863	29.36266725645977	23.178371823131652
29	21.916280857486274	23.21490254745888	28.293998606696636	26.574817988358213
30	23.83285992496705	28.626005733915505	25.72823294839393	21.81290139272351
31	24.412215675408362	30.529248187753012	23.012533931741388	22.04600220509724
32	29.73692079956726	24.327225826973592	23.802127728310815	22.13372564514833
33	25.18515941395109	27.263765911781203	24.369223734533463	23.181850939734243
34	24.879162825142085	25.827139263239076	24.407825361600327	24.885872550018515
35	24.80378196541924	24.48834206011748	27.37261255977661	23.335263414686676
36	25.897218611948446	24.98950052311003	26.90665944335791	22.206621421583613
37	27.53637954842723	26.996453786148646	25.005902072807974	20.461264592616153
38	22.09065086816385	26.138603035280727	29.402511425170413	22.36823467138501
39	24.100172050599607	28.460913367511516	23.242238463622108	24.196676118266772
40	26.819018839416405	22.347939824536553	27.657651612860473	23.175389723186573
41	23.604729279168456	22.750191972683965	27.19393507140058	26.45114367674699
42	23.575985149142273	24.966720592974006	24.247206145113953	27.210088112769764
43	21.7338757441789	22.818780271420795	28.509372491619057	26.937971492781248
44	25.330950966821653	22.915284339087957	26.466054176472394	25.287710517618002
45	21.80942227612092	25.999024190629083	25.750267353543688	26.441286179706314
46	22.12295695090221	25.826973591019904	25.2429790184418	26.807090439636085
47	24.02487402698635	23.3437126978644	29.20130251498712	23.430110760162126
48	22.57449658425302	22.649960280085455	31.229461822079635	23.54608131358189
49	22.015932697317684	22.83915795437884	27.609026816533756	27.53588253176972
50	20.865421971284036	23.767170890065714	30.879562095190288	24.487845043459966
51	23.382397161040853	23.40890471610823	28.084423249445205	25.124274873405717
52	22.905841022595204	21.525791436900008	28.397460907568984	27.170906632935804
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	166.0
1	130.5
2	95.0
3	89.5
4	84.0
5	106.0
6	128.0
7	199.0
8	270.0
9	290.5
10	311.0
11	358.0
12	405.0
13	483.5
14	655.0
15	748.0
16	685.0
17	622.0
18	741.0
19	860.0
20	1020.0
21	1180.0
22	1677.5
23	2175.0
24	3374.5
25	4574.0
26	5116.5
27	5659.0
28	6826.5
29	7994.0
30	10177.0
31	12360.0
32	15476.0
33	18592.0
34	21589.0
35	24586.0
36	29177.5
37	33769.0
38	35519.5
39	42634.5
40	47999.0
41	60845.0
42	73691.0
43	83379.5
44	93068.0
45	107549.0
46	122030.0
47	123335.5
48	124641.0
49	112474.0
50	100307.0
51	86623.5
52	72940.0
53	69437.5
54	65935.0
55	63784.5
56	61634.0
57	60552.5
58	59471.0
59	60794.5
60	62118.0
61	54719.0
62	47320.0
63	43564.0
64	33744.0
65	27680.0
66	24355.0
67	21030.0
68	17574.0
69	14118.0
70	10664.0
71	7210.0
72	6845.5
73	6481.0
74	4797.0
75	3113.0
76	2508.5
77	1904.0
78	1488.0
79	1072.0
80	924.5
81	777.0
82	467.5
83	158.0
84	152.0
85	146.0
86	130.0
87	114.0
88	63.0
89	10.0
90	8.0
91	7.5
92	7.0
93	3.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1207203.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.702751965208094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.62799723601702	11.295736637369709
2	12.68792053149308	4.238463791836003
3	5.6142728047969905	2.8132141837061186
4	2.972901424778425	1.9862254046035113
5	1.8365134748011431	1.5337414525182969
6	1.157585071453412	1.160091379626842
7	0.8441983963017765	0.9870305495998514
8	0.59593249943836	0.7962970180900352
9	0.45325717151540984	0.6813577901046307
>10	3.3181698611431547	11.80898299531632
>50	1.1923249394950126	14.69681909546445
>100	1.6850334975030312	44.12485083470442
>500	0.007442727462469219	0.8338156422522842
>1k	0.005954181969975375	2.585566087564533
>5k	4.961818308312813E-4	0.45780713724297967
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCTT	5524	0.4575866693505566	TruSeq Adapter, Index 21 (95% over 23bp)
GTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	4012	0.3323384716572109	TruSeq Adapter, Index 15 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	3827	0.3170137913838849	TruSeq Adapter, Index 21 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	3789	0.31386601921963414	TruSeq Adapter, Index 15 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	3578	0.2963876000970839	TruSeq Adapter, Index 21 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	2934	0.24304114552399225	TruSeq Adapter, Index 15 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	2891	0.23947919281181376	TruSeq Adapter, Index 21 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	2711	0.22456869308641547	TruSeq Adapter, Index 15 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	2059	0.1705595496366394	TruSeq Adapter, Index 21 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1709	0.1415669112816983	TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	1298	0.10752127024203882	TruSeq Adapter, Index 15 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1218	0.10089438147519514	TruSeq Adapter, Index 21 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.48003525504823963	0.0
2	0.0	0.0	0.0	1.020706542313099	0.0
3	0.0	0.0	0.0	3.5472907207818403	0.0
4	0.0	0.0	0.0	4.057726828047976	0.0
5	0.0	0.0	0.0	4.948463514421353	0.0
6	0.0	0.0	0.0	7.639560206526989	0.0
7	0.0	0.0	0.0	7.961378492266835	0.0
8	0.0	0.0	0.0	8.627794993882553	0.0
9	0.0	0.0	0.0	9.054815138796043	0.0
10	0.0	0.0	0.0	10.374311528384208	0.0
11	0.0	0.0	0.0	11.526230468280811	0.0
12	0.0	0.0	0.0	12.027968784040464	0.0
13	0.0	0.0	0.0	12.25104642715434	0.0
14	0.0	0.0	0.0	12.380436430326963	0.0
15	0.0	0.0	0.0	12.559859443689255	0.0
16	0.0	0.0	0.0	13.043456651449674	0.0
17	0.0	0.0	0.0	13.745492680187176	0.0
18	0.0	0.0	0.0	14.52324091308587	0.0
19	2.4850832875663827E-4	0.0	0.0	14.980330565778912	0.0
20	2.4850832875663827E-4	0.0	0.0	15.551071360823325	0.0
21	4.1418054792773047E-4	0.0	0.0	16.2945254443536	0.0
22	4.1418054792773047E-4	0.0	0.0	16.933688865915673	0.0
23	4.970166575132765E-4	0.0	0.0	17.666871271857342	0.0
24	5.798527670988227E-4	0.0	0.0	18.192383551068048	0.0
25	5.798527670988227E-4	0.0	0.0	18.65212395926783	0.0
26	5.798527670988227E-4	0.0	0.0	19.050565646374306	0.0
27	5.798527670988227E-4	0.0	0.0	19.44768195572741	0.0
28	5.798527670988227E-4	0.0	0.0	19.90212085291372	0.0
29	5.798527670988227E-4	0.0	0.0	20.34289179201841	0.0
30	5.798527670988227E-4	0.0	0.0	20.811909844491773	0.0
31	5.798527670988227E-4	0.0	0.0	21.231971756200075	0.0
32	5.798527670988227E-4	0.0	0.0	21.63770302095008	0.0
33	5.798527670988227E-4	0.0	0.0	22.095372526410223	0.0
34	6.626888766843687E-4	0.0	0.0	22.501517971708154	0.0
35	6.626888766843687E-4	0.0	0.0	23.011871242864704	0.0
36	6.626888766843687E-4	0.0	0.0	23.424974921367824	0.0
37	6.626888766843687E-4	0.0	0.0	23.836918894336744	0.0
38	6.626888766843687E-4	0.0	0.0	24.256069608839606	0.0
39	6.626888766843687E-4	0.0	0.0	24.674226290027445	0.0
40	6.626888766843687E-4	0.0	0.0	25.097850154447926	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	6.311582E-4	46.0	24
AACCGTC	20	6.311582E-4	46.0	14
AGCGTAC	30	1.8614828E-6	46.0	40
GGTACGC	20	6.311582E-4	46.0	34
GTCGCAA	30	1.8614828E-6	46.0	27
ACACGTC	20	6.311582E-4	46.0	38
TACCCCC	25	3.4172594E-5	46.0	37
CGTATTG	20	6.311582E-4	46.0	1
CGTATAG	65	0.0	46.0	1
GGTCGAC	20	6.311582E-4	46.0	13
CCGATTT	20	6.311582E-4	46.0	11
CCGATTG	60	0.0	46.0	44
CACGGGT	20	6.311582E-4	46.0	32
CAACGGC	30	1.8614828E-6	46.0	24
CACGGCA	25	3.4172594E-5	46.0	36
CGACGGT	30	1.8614828E-6	46.0	28
CCCTATG	25	3.4172594E-5	46.0	11
TCCGATA	45	3.092282E-10	46.0	20
CTATCGT	20	6.311582E-4	46.0	21
GCGAAAC	20	6.311582E-4	46.0	24
>>END_MODULE