FastQCFastQC Report
Thu 26 May 2016
SRR1545583_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545583_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1528056
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCTT49640.32485720418623404Illumina Single End Adapter 1 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC35750.23395739423162504No Hit
GCCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC35260.2307507054715272No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT30580.2001235556812054No Hit
TGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC28020.18337024297538834No Hit
TTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC25900.16949640589088358No Hit
ATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC22280.14580617464281415No Hit
AGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC17750.11616066426884879No Hit
GGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC15750.10307213871742921No Hit
ACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC15290.1000617778406027No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACG351.019871E-746.00000427
TCGTATC351.019871E-746.00000412
TCGTAAT351.019871E-746.00000432
CGTAATC351.019871E-746.00000433
AGCGTAC206.3121103E-446.020
CATGCGT253.417689E-546.018
TAACGTA206.3121103E-446.046
CGATTAG253.417689E-546.01
TTATCGC206.3121103E-446.026
GTATCGA206.3121103E-446.014
TCCCGTA206.3121103E-446.020
TTACGAC206.3121103E-446.037
TTACCGG453.110472E-1046.02
TTCGATC253.417689E-546.021
AATACGC253.417689E-546.035
AATACCC206.3121103E-446.039
ATCGCAA206.3121103E-446.028
GCGTACA253.417689E-546.032
ACGAATC206.3121103E-446.034
ACGCGTT206.3121103E-446.041
Produced by FastQC (version 0.11.5)